eChapters

ABSTRACT

Immature zygotic embryos were isolated from field grown papaya fruits harvested at 90-105 d post-anthesis. The embryos were placed on half-strength Murashige Skoog (MS) medium supplemented with 2, 4-D (2.0 mg l^-1) and cultured under dark for a period of 2 months. The frequency of callus formation was around 66%. After 2 months, calli were transferred onto a half-strength MS basal medium and cultured for 3 months with frequent subculturing (once in 3 wk) on the same medium. The frequency of somatic embryo formation was around 36.6%. The somatic embryos on MS medium supplemented with BAP (0.4 mg l^-1) and NAA (0.02 mg l^-1) germinated and produced shoots. The emerging shoots of 2-3 cm height with 2-3 trilobed leaves were placed onto an MS basal liquid medium with IBA (1.0 mg l^-1) for root induction. The plants developed with shoots and roots were planted in a pot-mixture (sand:soil:FYM) containing VAM.

ABSTRACT

Shoot organogenesis from several explant types excised from in vitro grown plants of local elite brinjal (Solanum melongena L.) cv. Co2 was studied in response to the supplementation of thidiazuron (TDZ), BAP, IAA and NAA. Cotyledons and leaves were found to be the most responsive explants. High shoot bud induction rate (>50 shoot buds/explant) was achieved in the presence of 0.044 mg/l TDZ. The organogenic calli were subcultured in the same shoot bud induction medium for shoot development. Among different growth hormones, TDZ @0.044 mg/l induced more number of shoots per explant compared to BAP, IAA and NAA combinations. Efficient rooting was observed on half strength MS medium supplemented with 0.5 mg/l IAA. Higher percentage of rooting was achieved only when the well developed shoots were directly transferred to soil.

ABSTRACT

Papaya (Carica papaya L.) is an important crop in the tropics and subtropics for export of fruits and above all, for local consumption. Cultivation of papaya faces with problems due to its heterozygosity, dioecious nature and susceptibility to a large number of viral diseases. Crossing between two hermaphrodites will produce the highest ratio of fruit bearing female trees, which is of homozygous dominant andromonoecious types which are always letha l due to po st fertilization barriers leading to their abortion. The gynodioecious varieties of papaya have the M1M1 forms while selfing and hence for explant selection , gynodioecious varieties like Sunrise solo and CO7 were prefered Embryo culture is one of the earliest forms of in vitro culture applied to solve practical problems and is probably the tissue culture techniques that has proven of greatest value to breeders. Ovule culture is practised in a case when small (or) young embryos that abort at early stages of development and are difficult to isolate. This necessiates the development of a protocol for initiation, multiplication and regeneration of ovular callus of papaya.

ABSTRACT

The present experiment was conducted in order to develop a protocol for rapid in vitro propagation of pomegranate Cv. Mridula. Shoot tip and nodal segment explants selected from mature plant were cultured in MS medium supplemented with NAA and BAP in different concentration and combinations. To control phenolic browning, sub- culturing of cultures was under taken at an interval of first and third day after inoculation. The MS medium supplemented with NAA 0.4 mg/l + BAP 1.0 mg/l showed highest percentage of shoot differentiation with 77.77 per cent and 81.25 per cent response from shoot tip and nodal segment explant respectively. In the same media, earlier shoot initiation as well as more number of multiple shoots/culture were obtained. The shoots when elongated up to 2.5 - 3.0 cm length were transferred for rooting in the ½ strength MS media supplemented with auxins. There was no significant difference for root induction among the type of auxin used either NAA, IBA or both in combination. The concentration of auxins 0.5 mg/l was found to be optimum. Rooted plantlets after hardening in green house gave 49 per cent survival.

ABSTRACT

Direct shoot organogenesis from several explant types excised from in vitro grown plants of local elite tomato cv. PKM1 was studied in response to zeatin, BAP, IAA and NAA. Cotyledonary leaves were found to be the most responsive explants. High shoot bud induction rate (>25 shoot buds/cotyledonary explant) was obtained in the presence of 2 mg/l zeatin. The organogenic calli were subcultured onto the same shoot bud induction medium for shoot development. Among different growth regulators tested, zeatin @ 2 mg/ l induced more number of shoots per explant compared to BAP, IAA and NAA combinations. Rooting was efficiently induced on half strength MS basal medium supplemented with 0.5 mg/l IAA. The effects of various growth hormones on tomato regeneration on rooting were discussed.

ABSTRACT

Vanilla (Vanilla planifolia Andrews) is a high value spice crop having immense potential for cultivation in India as a source for natural vanillin. Though the crop was introduced way back in 1835, its cultivation gained momentum only in the recent past due to various reasons. Lack of sufficient good quality planting materials is one of the constraints for expanding the area under the crop. Conventionally vanilla is propagated by vine cuttings, but this method is inadequate to have large number of planting materials in a short span of time. So, micropropagation was attempted and proved to be highly successful. In the present study, performance of vanilla plants raised from tissue culture plantlets and vegetative cuttings was assessed on large-scale in planters’ field covering a total area of 111 ha in Kerala(68ha), Karnataka(28ha) and Tamilnadu(15ha) with the financial assistance of the Department of Biotechnology, New Delhi under the project entitled “Tissue culture Vanilla : Product Plan“. Disease free elite accessions of vanilla identified by the Indian Cardamom Research Institute (Spices Board), Myladumpara from its germplasm repository were used as mother plant source for tissue culture multiplication and also for raising the nursery for producing vegetative cuttings. The field planting for the demonstration plots was carried out during 1996-1999 on a cluster approach basis having 400 plants in each unit or plot of 0.25 ha. Accordingly a total of 444 evaluation plots were laid out under the project covering the aforesaid three states. Out of the 400 plants in a plot, 80% were nursery hardened grown up tissue culture plants and the balance( 20% )vegetative cuttings of comparable length. Observations on growth and yield attributes such as vine length, number of leaves per vine, internodal length, number of beans per vine, bean size, yield etc. revealed that performance of tissue culture plants is on par with that of conventional plants raised from vegetative cuttings of comparable length. It is also observed that if good management practices are adopted, tissue culture plants perform better at the full bearing stage of the plant. This proves that tissue culture plants of vanilla can be popularized as a cost effective and faster source of planting materials compared to conventional vegetative cuttings. As the demonstration plots were distributed under varied agroclimatic situations, the influence of different weather parameters on yield was also ascertained. Further, the project could convince the farmers about the comparable performance of tissue culture plants and that is quite evident from the ever increasing request for tissue culture vanilla plants received by the Spices Board.

ABSTRACT

Dissected shoot tips from healthy suckers of banana var. Meitei Hei (local variety) were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators viz., NAA and BAP. After 15 days of incubation 50% of the shoot tips turned green in the medium with 5.0 mgl^-1 BAP. A maximum of 85.7% of the explants produced phenolic exudates in MS + 5.0 mgl^-1 BAP. After one month the explants were subcultured in fresh medium with the respective concentrations and combinations of plant growth regulators. After 75 days of incubation an average of 2.20 microshoots were induced in 78.57% of the explants in MS + 5.0 mgl^-1 BAP. The regeneration of shoots, leaves and roots was more proportionate in MS + 0.1 mgl^-l NAA + 0.5 mgl^-1 BAP. Under the treatment the microshoots had an average height of 1.19 cm with 4.13 leaves and 2.40 roots. The multiple microshoots were separated and subcultured on MS + 0.1 mgl^-l NAA + 0.5 mgl^-1 BAP for proper development. After 120 days the completely regenerated plantlets were hardened under green house environment.

ABSTRACT

Vanilla planifolia Andrews - the high value, exotic spice crop has recently gained wide acceptance in the southern and northeastern states of the country. Being an orchid, germination of naked embryo is limited and this favours genetic uniformity. An attempt was made to induce genetic variability in Vanilla through biotechnology tools for its exploitation in crop improvement programmes. In vitro embryo culture and mutagenesis were applied for the purpose. Plantlets were multiplied, hardened, planted out and evaluated for morphological, biochemical and molecular markers. Great interclonal variability was observed in the in vitro derived plantlets. The intraclonal variability was very low in tissue culture derived regenerants. Isozyme and RAPD assay confirmed genotypic variation. Six superior clones were identified and distributed to farmers for further evaluation.

ABSTRACT

Astudy was undertaken to evaluate the performance of tissue culture derived plants vis a vis open pollinated seedlings of cardamom (Elettaria cardamomum Maton). A total of 102.5 ha (units) covering 50 ha in Kerala, 37.5 ha in Karnataka and 15 ha in Tamil Nadu was selected for the present study out of which each demonstration plot (unit) had an area of one hectare in which 80% of the plants are tissue cultured and 20% are open pollinated. Elite cardamom clones were subjected to in vitro multiplication and their respective open pollinated seedlings were compared. The planting was initiated in 1989 and completed with 1991 in all the zones identified. Plant growth characters such as number of tillers, number of bearing tillers and panicles per clump were recorded and compared. The yield was recorded in 1993-94 and 94-95 seasons after its stabilisation. Results indicated that tissue culture plants performed better compared to open pollinated seedlings with regard to growth attributes and yield irrespective of the seasons and zones. In Kerala region tissue culture plants exhibited a yield increase of 24.3 and 36% over open pollinated seedlings for the 1989 and 1990 plantings respectively. Similar trend was observed in Tamil Nadu for 1989 and 1990 planting where the percentage increase in yield became 130 and 21 respectively. In Kerala, the yield performance of tissue culture plants and open pollinated seedlings was on par during 1990 planting but in the subsequent year (1991) tissue culture plants showed 24% increase in yield compared to their respective open pollinated ones. Further, the study enabled to identify cardamom clones suited to in various zones of the cardamom tract. Accordingly 14 clones with a yield potential of over 750 kg/ha under moderate management were identified and this indicated that cardamom clones are highly location specific in their performance.

ABSTRACT

Recognizing the need to apply plant tissue culture tools for generating planting materials of important spice crops especially vanilla, the Spices Board started Biotech Production Unit at its head quarters building at Kochi with an annual production potential of 8 lakhs plantlets. Complete tissue culture protocols were developed and scaled up for large scale production of vanilla and other spices viz. large cardamom, small cardamom and tree spices. The laboratory is equipped with all modern facilities and is established in an area of about 2000 sft. Department of Biotechnology, Govt. of India also participated in the venture by sanctioning a project entitled “Regional centre for large scale production of tissue culture plantlets of vanilla and other spices” with a financial out lay of Rs 95 lakhs equally shared by DBT, New Delhi and Spices Board, Cochin.

At present there is a stock of over 3.00 lakh vanilla cultures in the lab and already 1.25 lakh plant lets were sent for hardening. The Spices Board, in coll aboration with “Kudumbashree”, a poverty eradication mission of Govt. of Kerala, extend short term training in tissue culture techniques to undergraduates from ‘Below Poverty Line’ (BPL) families and engage them as long term trainees in the Biotech Production Unit. The Board also coordinates and extends support to Kudumbashree members for, establishing and maintaining hardening facilities for TC plantlets thus providing means for empowering women from BPL families.

ABSTRACT

Jasmine is one of the economically most important flower crops cultivated for its fragrant flowers and valuable essential oil. In vitro studies to explore the possibility of regeneration of plantlets from different explants and to undertake biochemical analysis to detect the flavour compounds if present in the in vitro cultures were taken up with local varieties of Jasminum grandiflorum.

Among the different explants tried, leaf bits were found ideal for callus induction, followed by petals and shoot tips. MS medium with 2, 4-D at 1.25 ppm was the suitable medium for callus induction in leaf explants. Miller medium with NAA at 1.25 ppm and BAP at 1.25 ppm was the suitable medium for callus induction in petal explants collected from 18 days old buds of local varieties of Jasminum grandiflorum. Among the different media combinations tried, MS medium supplemented with 0.2 ppm NAA and 0.3 ppm GA₂ is the best medium for callus induction in shoot apex explants of 10mm size. Direct regeneration of plants was obtained in 10 per cent of shoot apex explants cultured in MS medium supplemented with 0.2 ppm GA₁ and 3 ppm BAP in 60 days. A maximum of 95 per cent embryogenicity is induced in leaf callus transferred to MS basal medium supplemented with 1.5 per cent sucrose from MS medium with 2, 4-D at 1.0 ppm.

The peroxidase assay indicated that embryogenic calli had six isoperoxidases while the non-embryogenic had only two. Biochemical analysis of the callus samples showed high Phenylalanine ammonia lyase and phenol indicating the initiation of phenolic pathway, which branches out and ultimately leads to the synthesis of flavour compounds in callus.

ABSTRACT

Donor-recipient protoplast fusion vis-à-vis cybridization is an effective technique to transfer plasmon-coded traits in cultivated tetraploid (2n=4x=48) potato (Solanum tuberosum L.) from its diploid (2n=2x=24) cross-incompatible wild relatives. However, the method, although attractive, is difficult in terms of not only inactivation of nuclear and plasmonic genomes of the participating donor and recipient protoplasts, but also post- fusion culture and regeneration of putative cybrids. Here, we report an improved method for donor-recipient protoplast fusion and subsequent post-fusion culture and regeneration of electrofused protoplasts in potato. In the present experiment, Solanum verrucosum Schlechtd. (2n=2x=24) accessions CPC 1339 (CGN No. 17774) and HAW 2246 (CGN No. 17766) containing sensitive [Tr^s] plasmon for tetrad-type cytoplasmic male sterility were used as donors and S. tuberosum subsp. tuberosum cvs Kufri Ashoka, Kufri Chipsona-1 and Professor Broekema as recipients. For nuclear inactivation, the in vitro-cultured donor plants were exposed to 1000 Gy of unfiltered γ-rays before protoplast preparation using a revised protoplast isolation medium (PIM) containing 2.0 % Cellulase ‘Onozuka’ RS and 0.25 % Macerozyme R-10. The recipient protoplasts were treated with antimetabolite iodoacetate (1.0 mM ) for 45 min at 10 °C for chondriome inactivation. The protoplasts were aligned in an amplitude of 300 Vcm^-1 for 0.5-1.0 s before being electrofused by one DC pulse of 600-800 Vcm^-1 for 40 μs using a Cell Electrofusion Processor FPH 01001 (Bioelectronics Corporation, Troy, Michigan). The fused protoplasts were immobilized in alginate-plates, and cultured on modified VKM medium containing 0.3 M glucose at about 400 mOsmol (pH 5.6). After 8-10 weeks, the developing protocalluses were dissociated from alginate-plates and cultured for regeneration on MS medium supplemented with varying concentrations of zeatin/ zeatin riboside and gibberellic acid. The protocalluses derived from electrofusion between donor S. verrucosum accession CPC 1339 and recipient S. tuberosum cv. Kufri Chipsona-1 could be successfully regenerated into plantlets. Morphological as well as molecular-genetic characterization of regenerated microplants, both for nuclear and chondriome genomes, confirmed the putative cybridity as well as established the extent and pattern of mitochondrial recombination following donor-recipient protoplast fusion. The results have been discussed in relation to donor-recipient protoplast fusion approach and its potential application in potato genetic improvement.

ABSTRACT

The investigation was carried out to develop protocol for micropropagation of acid lime (Citrus aurantifolia S) var. Sai-Sharbati. Shoot tip and nodal segment explants were obtained from axenically grown seedlings having 21 to 30 days age. Direct regeneration of shoots, roots and whole plant without intervention of callus was obtained from both the explants on MS basal media. In both the explants 100 per cent shoot proliferation was induced in nutrient medium MS + BA 0.25 mg/l + ME 200 mg/l. The shoot proliferation rate was more (5.0) in nodal segment as compare to the shoot tip explant (4.33). Regenerated shoots of more than 2.5 cm length with minimum of 4 leaves were transferred for rooting. Half strength MS medium supplemented with IBA 1.0 mg/l was best for root induction and root initiation within 15.66 days. More than 75 per cent plantlets were survived after hardening period of four weeks.

ABSTRACT

Immature seeds of Garcinia indica were excised from the green fruits and cultured on Woody Plant Medium (WPM) with a combination of auxins and cytokinins. Somatic embryos were obtained on the media supplemented with BAP (0.5-5.0 mg/l) alone or in combination with NAA 90.5 mg/l) with 80 per cent frequency within a period of 2-3 weeks. Subculture of primary embryos on maturation medium containing BAP (3.0 mg/l) supplemented with IAA (0.5-1.0 mg/l) and / or Kinetin (1.0 mg/l) gave rise to clusters of secondary somatic embryos. In the subsequent subculture on hormone free half strength WPM the embryo clusters germinated with repetitive secondary embryogenesis. About 70 per cent somatic embryos germinated into complete plantlets, which were successfully transferred to the green house conditions.

ABSTRACT

The totipotency of triploid endosperm tissue is exploited in many vegetatively propagated crops. Hevea brasiliensis, the most important commercial source of natural rubber, is a highly heterozygous perennial tree with a long breeding cycle. Conventional method of triploid production by crossing a diploid with an artificially induced tetraploid is a lengthy and laborious process. Hence in vitro regeneration of plants from endosperm tissue provides an easy and direct approach for the production of large number of triploids. Hevea fruits at different developmental stages were collected and sterilized using 0.1% HgCl₂ for 15 minutes. Seeds were dissected out and the embryos and inner integuments were removed. Endosperm is cut into small pieces and cultured over MS medium supplemented with various levels of sucrose, phytagel and growth regulators viz BA, Kin thidiazuron, 2,4-D, IBA, NAA, zeatin & GA. Organic supplements viz. yeast extract, casein hydrolysate, coconut milk and banana powder were used for callus and embryo induction. Optimum callus induction was obtained in media supplemented with 2,4-D (2.0 mg ^-1) and Kinetin (3.0 mg^-1). On further subculture of the primary calli to medium containing zeatin (1.0 mg ^-1), embryogenic calli could be obtained. Embryos were induced in MS medium supplemented with ABA (0.3 mg ^-1) , Kin (0.3 mg ^-1) and GA (0.5 mg ^-1). Maturation, germination and bipolar differentiation of the embryos were achieved in MS medium devoid of growth regulators. Root tips from the germinating embryos were subjected to cytological analysis and found to be triploids (3n=54). Regeneration from Hevea endosperm is being reported for the first time. The results indicates that like many other crops, Hevea endosperm responds well under in vitro conditions and endosperm culture could be utilized as a viable technique for triploid plant production.

ABSTRACT

Microbial contamination is perceived as the most serious threat faced by plant tissue culture research laboratories and commercial production units. A culture is often viewed as contaminated when obvious microbial growth incited by fungi, yeast or bacteria is noticed on the culture medium. On the other hand, there can be situations when microbes may be present in a covert or latent form with serious side effects on the cultures. The present investigations were taken up with the objectives of elucidating the cause of poor growth, lack of regeneration response or modification of rooting observed with visibly clean cultures of different horticultural crops. Apparently clean cultures of grape, banana, watermelon, eggplant, gerbera and chrysanthemum of recent origin or after long-term in vitro culturing were used in this study. Indexing the medium of such cultures using fungal/ yeast and bacteriological media brought out the covert association of one or more bacterial types with varying proportions of incidence in such cultures. Indexing of tissue revealed bacterial presence in different parts of in vitro plantlets. In some instances, bacteria showed endophytic colonization while being not detectable in the tissue culture medium subsequent to a disinfection or antibiotic treatment. Such bacterial association appeared to be the cause of in vitro decline observed with long-term micropropagated cultures, lack of reproducibility of tissue culture protocols, failure of in vitro rooting etc. besides being a threat to in vitro gene banks and germplasm exchange.

ABSTRACT

Techniques could be standardized for the induction, initiation, maturation and germination of somatic embryoids from the nucellar tissue isolated from 30-45 day old fruits of mono embryonic mango variety Neelum and poly embryonic variety Vellari Manga. The treatment half strength Murashige and Skoog basal medium containing 2,4-D 5.0 mgl^-1, GA3 5.0 mgl^-1, glutamine 400 mgl^-1, coconut water 200 mll^-1, activated charcoal 500 mgl^-1, sucrose 60 gl^-1and agar gl^-1was found to be the best in induction of embryogenic callus. The treatment 2,4-D 4.0 mgl^-1 + BA 1.0 mgl^-1+ GA3 5.0 mgl^-1in half strength MS basal medium supplemented with glutamine 400 mgl^-1, casein hydrolysate 500 mgl^-1, sucrose 60.0 gl^-1, coconut water 200 mll^-1and agar 6.0 gl^-1was the best in initiating somatic embryos from the induced nucellus of the three varieties. In the variety Neelum BA (8.0 and 16.0 mgl^-1) and kinetin (8.0 to 32.0 mgl^-1) were as effective as 2,4-D in inducing somatic embryogenesis. Polyembryonic variety Vellari Manga showed better response to induction treatments than the mono embryonic varieties. B5 major salts with MS minor salts in combination with 40 gl^-1sucrose, 10 gl^-1PVP, 200 mll^-1coconut water, 5.0 mgl^-1 ABA, 100 mgl^-1 casein hydrolysate and 6.0 gl^-1agar were the best suited for the maturation of the somatic embryos. The positive influence of cobalt chloride, casein hydrolysate and coconut water on the germination of embryoids of both mono and poly embryonic mango varieties could be well established. A basal medium consisting of B5 major salts and MS minor salts supported the highest percentage of germination of somatic embryoids in Neelum and Vellari Manga. However, abnormalities in germination were observed in most of the cases. Excellent germination of somatic embryos was observed in a liquid medium consisting of half strength B5 macro salts and full strength MS micro salts and supplemented with GA3 1.0 mgl^-1+ glutamine 400 mgl^-1 + sucrose 30gl^-1.

Waste is not actually waste but is the by-product of various activities of human life. The unclean handling of these wastes creates problem for public health departments and local bodies. The classification of waste depends on the agencies that classify these wastes according to the area of interest and method of disposal viz. medical field, agricultural field, local bodies, industries etc.

In the present paper, the emphasis is given to the classification based on agricultural activities which includes a) Agricultural marketing waste b) Meat and fish marketing waste.

ABSTRACT

Commercial propagation using tissue culture techniques started in seventies for orchids in USA. Since then, the science and technology of tissue culture have advanced particularly after the development of the most widely used medium by Murashighe and Skoog (1972). The science has led to the development of the fascinating area of gene transfer. Today, micropropagation protocols are available for almost all horticultural crops. But, there are certain grey areas where research need to be intensified to solve the problems confronting certain difficult to propagate species belonging to plantation and spice sectors. The major area that needs to be addressed is recalcitrance.

ABSTRACT

The Plant Growth Promoting Rhizobacteria (PGPR) is a group of rhizosphere-colonizing bacteria producing substances which increase the growth of plants and/or protect plants against pathogens. The use of PGPR has become rather common practice and is on the rise in many regions of the world. Inoculation with PGPR also aims at reducing the application of potent ially pollut ing chemical fertilisers and pesticides. Biocont rol microorganisms have unquestionable potential for managing plant diseases, insect pests and nematodes besides increasing crop productivity. Interest in biological control has recently been intensified because of imminent bans on effective pesticides, widespread development of pesticide resistance in insect pests and plant pathogens and a general need for more sustainable pest control strategies. Active and long-term colonization of the root surface of the plant and effective expression of disease-suppressive traits are generally considered prerequisites for successful suppression of horticultural crop diseases by rhizobacteria. The important mechanism of biocontrol by PGPR involve competition and production of antibiotics such as 2,4-diacetylphloroglucinol (DAPG), pyoluteorin (PLT), pyrrolnitrin, phenazine-1-carboxylic acid (PCA), 2^-hydroxy phenazines and phenazine-1-carboxamide (PCN). Siderophores, including pyochelin and pyoverdine which chelate iron and other metals also contribute to disease suppression by conferring a competitive advantage to biocontrol agents for the limited supply of essential trace minerals in natural habitats. Induced systemic resistance (ISR) is a state of increased defensive capacity developed by the plant when appropriately stimulated, through activation of latent resistance mechanisms induced by diverse agents including rhizobacteria. Microplants are first grown in vitro under aseptic conditions devoid of microorganisms. They are then transferred to a post vitrum substrate for acclimatization. By creating a beneficial rhizosphere in microplants at an early stage, the plants could be better protected against biotic and abiotic stress that occurs in the greenhouse or in the field. The beneficial rhizosphere can be created by biotization of microplants with PGPR. The search for PGPR to control postharvest decay of fruit crops has been actively pursued and promising strategies have been practiced nowadays. Biological control of postharvest diseases of perishable horticultural crops with PGPR is complex and involves a number of mechanisms to suppress the development of the disease. PGPR, being a potential candidate in disease management through multiple mode of action, it becomes highly imperative to know its different role in the management of plant pathogens.

Introduction

The common mycorrhizal association in most of the plants is the arbuscular type occurring in the majority of agricultural and horticultural crops. Arbuscular mycorrhizae (AM) are formed by non-septate Zygomycetous fungi belonging to the genera Glomus, Gigaspora, Acaulospora, Sclerocystis, Entrophospora, Scutellospora, Archaeospora and Paraglomus in the order Glomales. These fungi are obligate symbionts and have not been cultured on nutrient media. These endophytes are not host specific although evidence is growing that certain endophytes may form preferential association with certain host plants. Several investigations indicted that even in unsterile soils plants do respond to inoculation with efficient strains of arbuscular mycorrhizae. Numerous experiments have been conducted in different parts of the world confirming this growth improvement. The increased growth of mycorrhizal plants is favoured in soils with low to moderate fertility. More specifically, soils favouring mycorrhizal growth stimulation usually have one or more essential nutrients, especially phosphorus, in limiting concentrations.

The mechanism of improved plant growth caused by mycorrhizal inoculation has been investigated by many workers. Greater soil exploration by mycorrhizal roots as means of increasing phosphate uptake is well established. They also improved the uptake other diffusion-limited elements like Zn, Cu, etc. The other beneficial effects are their role in the biological control of root pathogens, biological nitrogen fixation, hormone production and greater ability to withstand water stress (Bagyaraj, 1984).

Introduction

Increasing production of cereal crops, oil seed crops, and horticultural crops is becoming more important than ever before owing to increasing population and changing food habits of the people. The modern tools of biotechnology are being exploited to the fullest throughout the world for developing transgenic crop plants with improved traits. Already about 20 countries in the world are growing transgenic crops, predominately with traits such herbicide tolerance and resistance to insect-pests. However, there is a constant fear of pollen flow from theses transgenics to the neighbouring non-transgenic fields or to their weedy or wild relatives. It has become a matter of great concern to all biotechnologists to develop alternative strategies to prevent the pollen flow.

The best alternative strategy available today to circumvent the nuclear- transformation associated bottlenecks in developing tenable crop transgenics is the chloroplast transformation technology. This technology allows introduction and insertion of foreign genes into the chloroplast genome, called plastome. Chloroplasts are the site for photosynthesis in plants mostly present in leaves. In general called plastids, they are also present in other parts of the plant. For example, chromoplasts in coloured leaves and fruits, amyloplasts in starch storing organs, leucoplats in roots, and etioplasts in dark-grown seedlings. The plastids arise from proplastids - the undifferentiated form of plastids present in most plant cells. Just like nucleus, these plastids (and also mitochondria) contain their own genetic material on the chloroplast genome.

ABSTRACT

Of the total production of the crop yield, only 63 per cent is available for human consumption. The remaining is lost in more or less equal proportions to weeds, pests and diseases. Prior to genetic engineering, the exchange of DNA material was possible only between individual organisms of the same species. In the field of agriculture for instance, farmers have continuously modified naturally occurring species through seed selection and controlled breeding. With the advent of the recombinant DNA technology, it has however been possible to identify specific genes associated with desirable traits in one organism and transfer those genes within and beyond the boundaries of species into other organisms.

1. Genes for herbicide resistance

Modern agriculture has developed a range of effective herbicides to tackle the effects of weeds on crop yield. Some of the most useful of these are broad-spectrum herbicides because they are active against a wide range of weeds. However, these can only be used at times when the crop is not itself vulnerable to herbicide action. The development of herbicide-resistant crops therefore offers the opportunity to spray crops at the most effective time to kill weed species without damaging the crop plants. By definition, herbicides are much more toxic to plants than to animals. Therefore, it is not surprising that they generally affect ‘plant-specific’ biological processes. The herbicidal activity of many herbicides has been found to result from the specific inhibition of a single enzyme/protein. The following are four distinct strategies for engineering herbicide resistance: i. Overexpression of the target protein, ii. Mutation of the target protein, iii. Detoxification of the herbicide, using a single gene from a foreign source and iv. Enhanced plant detoxification.

ABSTRACT

The coastal ecosystem happens to be one of the most productive ecosystems. This ecosystem has been under different kinds of threats such as climate change and consequent rise in sea level, increase in population and depletion of natural resources. All this has given rise to an increase in the level of abiotic stresses such as salinity, alkalinity, and drought affecting the agricultural productivity in the coastal region. Salinization is posing an increasing problem in coastal and agricultural areas reducing plant productivity and yield. Salinity is one of the major abiotic stresses decreasing the plant productivity.

Salinity is a significant factor limiting agricultural productivity and affecting about 9x 10⁸ hectares worldwide. Poor quality of water for irrigation and current unsustainable irrigation practices has significantly resulted in salinization and other forms of soil damage. About one third of all irrigated land is affected by salt due to secondary salinization. It is estimated that 50% of all arable land will be salinized by the year 2050. The problem of secondary salinization is also becoming more serious as it represents loss of highly productive lands. Added to this is the problem of coastal salinity where intrusion of seawater into arable land due to erosion of coastal habitat has rendered it unsuitable for cultivation. Salt-affected soils (~11 million ha in South and Southeast Asia) are found along coastlines or in inland areas where evaporation is greater than precipitation.

ABSTRACT

The process of developing new crop varieties involves many steps and takes several years. In recent times, biotechnological advances have helped in reducing these considerably. Apart from the techniques of haploid culture and genetic engineering, one of the tools which is making it easier for scientists to select plant traits and develop new varieties is the use of molecular markers. Molecular markers can be used for (a) characterization of germplasm (b) varietal identification and clonal fidelity testing (c) assessment of genetic diversity (d) validation of genetic relationships and (e) marker- assisted selection. These have widespread applications in management of genetic resources as well as in breeding programmes. Marker-assisted selection has practically revolutionized breeding programmes. Traditionally, plant breeders have selected plants based on their visible or measurable traits called the phenotype. But this process can be difficult especially in the case of multigenic or quantitative traits. Molecular markers can be used to trace linkages with traits of importance. In the area of horticulture, this is a very useful tool in tree breeding, where the breeder has to wait for several years for phenotypic expression of the desired trait. A number of DNA marker technologies are now available and rapid advances are being made in the development of more sensitive marker technologies. The techniques involved, the various applications of DNA marker technologies in horticultural crops and future perspectives in the national context have been discussed.

ABSTRACT

Genetic improvement of crop plants through conventional plant breeding has made tremendous contributions to the breakthrough in the global agricultural production. Recently, an array tools and techniques in the field of molecular biology has become available for supplementing the conventional genetic approaches. Consequently, new integrated approaches are being designed. One of the approaches employs molecular markers for genome mapping, gene tagging and marker assisted selection (MAS). With the complete sequencing of whole plant-genomes and a large number of random cDNAs in many different crop species, newer opportunities are emerging. This presentation is an attempt to briefly describe the current applications of molecular markers and to highlight their future prospects.

Molecular markers

Molecular markers are heritable differences in nucleotide sequences of DNA at corresponding position on homologous chromosomes of two different individuals, which follow a simple Mendelian pattern of inheritance. These differences are detected employing two basic techniques: (a) Southern blot hybridization and (b) Polymerase chain reaction (PCR). Restriction Fragment Length Polymorphism (RFLP) is the first molecular marker system to be conceived and developed. It is based on the southern blot technique. Markers such as random amplified polymorphic DNA (RAPD), sequence tagged site (STS) and amplified fragment length polymorphism (AFLP) are generated by enzymatic amplification of DNA by extension of oligo nucleotide primers and therefore, are based on PCR. Single/simple nucleotide polymorphism (SNP) markers are based on DNA sequence differences as revealed by sequencing and assayed by various techniques.

Importance

Ajwain is an important seed spice and is also useful for its medicinal values. It is a traditional potential herb for curing many ailments in human and animals. It is usually used as a digestive aid, relieves abdominal discomfort due to indigestion and antiseptic. The seeds of ajwain is bitter, pungent and contain 2-4.4% brown coloured oil known as ajwain oil. The main component of this oil is Thymol, which is used as gastrointestinal ailment, lack of appetite and bronchial problems. Thymol is also used in toothpaste and perfumery (Joshi, 2000). Seeds are also used for curing abdominal pains, abdominal tumors and piles (Krishnamoorty and Madalgeri, 1999). In southern parts of India dry ajwain seeds are powdered and soaked in milk, which is then filtered and fed to babies as it relieves colic in babies. It can be used as digestive mixture in large animals.

Importance

Ginger is a perennial herbaceous monocotyledon, usually grown as annual, and is known to human as spice as well as medicinal crop. The word Zingiber is thought to come from the Sanskrit word singabera meaning ‘shaped like a horn’. It probably got its name because the rhizomes look like deer’s antlers. It first appeared in the writings of Confucius in the 5^th century B.C.

The economic part is the underground rhizomes, which is pungent and aromatic. It is valued for its medicinal properties and is useful against gastritis, dyspepsia, cold and cough. It is widely used in food, beverages, confectionery, etc. Baby ginger or young ginger results from early harvesting and is a gourmet quality product with a tender, low-fiber texture and is mostly used for pickling. Rhizome contains proteins, fibre, starch, essential oil, Zingiberol, Glutamic acid, Aspartic acid, Glucose, Fructose, and Sucrose. Most ginger varieties have about 3-6% crude fibre, 1.5-3.0% volatile oil and 4-10% oleoresin. The major pungent principle in ginger oleoresin is gingerol. Among the gingerols 6-gingerol, 8gingerol and 10-gingerol are very important in contributing the pungency. Gingerol (Hydroxy methoxy phenyl decanone) on storage gets converted to another compound called shogaol. The age of ginger oleoresin can be assessed based on the shogaol content. Chemically, gingerol is a relative of capsaicin, the compound that gives chilli its spiciness. Cooking ginger transforms gingerol into zingerone, which is less pungent and has a spicy-sweet aroma.

1. EXPERIMENT 1 : INDUSTRIAL USES OF IMPORTANT CHEMICALS

1. INTRODUCTION

Food is constantly being rated for its quality either consciously or subconsciously by all people. Consumers choose food on the basis of its quality and their individual likes or dislikes. The attractiveness of food is clearly a quality which is important both to the manufacturer and to the consumer. When consumers make a selection they basically look for food that is attractive in terms of colour, flavour and texture apart from other psychological and social factors, which they may associate with food. The nutritional quality, shelf-life/keeping quality and cost factor are other criteria which may affect their selection.

The distinctive and attractive colour of food is not only pleasing but is also an indicator of good quality and freshness. The senses of taste and odour help us in detecting and appreciating different flavours and deciding on whether we like the food. The feel and texture of food entering the mouth should be firm, juicy, crisp and are extremely important characteristics that dictate the acceptance of a food. Today’s consumers are discerning, demanding, and more knowledgeable about food. They expect safe products having good value and high sensory quality. Therefore, knowing consumer’s preferences and perceptions of the sensory characteristics of food and beverage products is vital to food manufacturers. Without appropriate evaluation, there is a high risk of market failure.

1. INTRODUCTION

India is a vast country having a geographical area of 328 million hectares with 15 distinct agro-climatic zones. Fruits and vegetables are one of the most important and fast growing sub-sectors as they form an indispensable part of healthy diet. It holds a unique position for growing a wide range of vegetables in its diverse agro-climatic conditions. World production of vegetables amounts to 486 million tons, while that of fruits is 392 million tons. India produces 159.5m MT tons of vegetables from an area of 9.07 million hectare annually (NHB, 2013). Productivity of vegetables in India presently is 16.7 tons per hectare. India produces 36 % green peas and 10% onion of world’s production. Onion, potatoes and green vegetables like okra, bitter gourd and green chillies also have good export potential. Being rich in vitamins, minerals, dietary fibre and natural antioxidants most vegetables are classified as the protective foods. However, owing to the presence of high moisture content and mostly being non acidic, the vegetables are highly perishable and needs to be handled properly and immediately after harvest. Processing of vegetables into different value added products is one of the most effective alternatives to reduce post harvest losses.

Vegetables are highly beneficial for the maintenance of health and disease prevention. An intake of about 280 grams of vegetables per day per person has been considered essential. Vegetable unlike cereals deteriorated quickly when stored for long periods because of the relatively high moisture contents. Huge amounts of losses take place between harvesting and consumption. Various causes are attributed to the deterioration process and it becomes essential to adopt integrated, low cost system of post harvest technology right from the pre-harvest stage.

Lipids are a class of compounds that are defined by how they are isolated, rather than by their composition. Lipids are molecules that can be extracted from plants and animals by low polarity solvents such as ether, chloroform, or even acetone. They are not appreciably soluble in water. Fats and the fatty acids from which they are made belong to this group as do oils, waxes and steroids. Hydrolyzable lipids are those that contain a functional group that will react with water. The functional group is usually an ester and the list of compounds includes neutral fats, waxes, phospholipids, and glycolipids. Non- hydrolyzable lipids lack such functional groups and include steroids and fat-soluble vitamins (e.g. A, D, E, and K).

Fats and oils are composed of triacylglycerols. These are compounds prepared by the union of glycerol (1,2, 3-tri- hydroxypropane) and 3 fatty acids to form a triester. Fats and oils belong to a group of biological substances called lipids. Lipids are biological chemicals that do not dissolve in water. They serve a variety of functions in organisms, such as regulatory messengers (hormones), structural components of membranes, and as energy storehouses. Fats and oils generally function in the latter capacity. Fats differ from oils only in that they are solid at room temperature, while oils are liquid. Fats and oils share a common molecular structure. The fats and oils contain three ester functional groups. Fats and oils are esters of the tri- alcohol, glycerol (or glycerine). Therefore, fats and oils are commonly called triglycerides, although a more accurate name is triacylglycerols. Triglyceride molecules contain mostly carbon and hydrogen atoms, with only six oxygen atoms per molecule. This means that fats and oils are highly reduced (un-oxidized). Oils are particularly common in seeds, where the stored energy helps seedlings during germination, until they can exploit solar energy through photosynthesis. Fatty acids contain an even number of carbon atoms, from 4 to 36, bonded in an unbranched chain. Most of the bonds between carbon atoms are single bonds. If all of these bonds are single bonds, the fatty acid is said to be saturated, because the number of atoms attached to each carbon atom is the maximum of four. If some of the bonds between carbon atoms are double bonds, then the fatty acid is unsaturated. When there is only one double bond, it is usually between the 9th and 10th carbon atom in the chain, where the carbon atom attached to the oxygen atoms is counted as the first carbon atom. If there is a second double bond, it usually occurs between the 12th and 13th carbon atoms, while a third is usually between the 15th and 16th.

1. INTRODUCTION

Proteins are polymers of amino acids. Twenty different types of amino acids occur naturally in proteins. Proteins differ from each other according to the type, number and sequence of amino acids that make up the polypeptide backbone. Proteins are the important constituents of foods for a number of reasons. They are a major source of energy, as well as containing essential amino-acids, like lysine, tryptophan, methionine, leucine, isoleucine and valine which are essential to human health, but which the body cannot synthesize. Proteins are also the major structural components of many natural foods, often determining their overall texture, e.g., tenderness of meat or fish products. Isolated proteins are often used in foods as ingredients because of their unique functional properties, i.e., their ability to provide desirable appearance, texture or stability.

Proteins are complex organic molecules made up of carbon, hydrogen, oxygen, and nitrogen. Most proteins also contain sulphur and phosphorus and also play an important role in food preparation. The final quality of many food products are influenced by how the proteins are treated while food is being prepared.

Food is composed of three main constituents viz; carbohydrates, proteins and fats and their derivatives. In addition to these constituents, inorganic mineral elements and diverse organic compounds like vitamins, pigments, flavouring compounds, enzymes, and acids are also present. Carbohydrates, proteins, fats and vitamins are organic in nature. The percentage of water is large in certain foods. The variation in structure, texture, colour, flavour and nutritive value is because of the varying proportions and arrangements of these constituents.

Carbohydrates are important in foods as a major source of energy, to impart crucial textural properties, and as dietary fiber which influences physiological processes. Digestible carbohydrates, which are converted into monosaccharides, which are absorbed, provide metabolic energy. Carbohydrates account for more than 70% of the caloric value of the human diet. It is recommended that all persons should limit calories from fat to not more than 30% and that most of the carbohydrate calories should come from starch. Non-digestible polysaccharides (all other than starch) comprise the major portion of dietary fiber.

1. TERMS RELATED WITH BROWNING

1. INTRODUCTION

Quality, i.e. the degree of excellence or superiority, is a combination of attributes, properties, or characteristics that each commodity value in of human food. The relative importance of each quality component depends upon the commodity and its intended use (e.g. fresh or processed) and varies among producers, handlers, and consumers. Appearance of quality, firmness and shelf-life are important from the point of view of wholesale and retail marketers. Consumers judge quality of fresh fruits and vegetables on the basis of appearance and firmness, flavour (eating) quality of the product. Consumers are also concerned about the nutritional quality and safety of fresh fruit and vegetables.

1. FRUIT CROPS

1.1 Physiological Disorders

A wide variety of physiological disorders are found in apple fruit, but the susceptibility varies with variety, pre-harvest factors and post harvest conditions. Disorders can be considered in three categories:

1. Disorders that develop only on the tree: Most important is watercore in which intercellular air spaces in the core and cortical tissues become filled with liquid, Presence of watercore in fruit at harvest creates problems in certain varieties such as ‘Delicious’ because fruit with moderate or severe watercore can develop breakdown during storage.
2. Disorders that develop on the tree and during storage: Bitter pit is a disorder characterized by development of discrete pitting of the cortical flesh. Rapid cooling, CA storage and application of postharvest calcium drenches may be able to reduce its occurrence.
3. Disorders that develop during storage: Senescent breakdown disorders, chilling disorders and disorders associated with inappropriate atmospheres during storage are common disorders. The most common disorders associated with temperature and atmospheres are superficial scald, soft scald, low temperature breakdown, brown core, internal browning, low O2 injury, and high CO2 injury.

I. POST HARVEST DISEASES

A. Post-Harvest Diseases of Fruits

1. INTRODUCTION

Grading and packing of fruits ensure the safe transportation from the farm to the storage and consumer centre, while reducing the damage during transportation. The properly graded fruits should be packed in such a way that they do not collide with each other during transportation. Throughout the entire handling system, packaging can be both an aid and hindrance to obtaining maximum storage life and quality. Before the fruit is packed in a suitable container/package it is graded according to the standard grades accepted in the market. Packaging is one of the important components after grading during the handling of fruits. Packaging is meant to protect the commodity by immobilizing and cushioning it, but temperature management can be made more difficult if packing material block ventilation holes. Packing material can act as vapour barriers and helps in maintaining higher relative humidity within the package. Packaging material such as trays, cups, wraps, liners and pads may be added to help immobilize the produce. A wide variety of containers such as wooden boxes, plastic crates, corrugated fiberboard boxes are important package used in the transportation and distribution of fruits in most of the developing countries.

1. INTRODUCTION

Harvesting is the process of gathering the crops from the fields at maturity. The harvesting of a commodity means the end of the growing season for a particular crop. Harvesting is the most labour-intensive activity of the growing season. Harvesting in general includes an immediate post-harvest handling, the actions taken immediately after removing the crop are pre-cooling, sorting, cleaning, packaging and up to the point of on-farm processing or shipping to the wholesaler or consumer.

Harvest timing is a critical decision that balances the likely weather conditions with the degree of crop maturity. Weather conditions such as frost, rain and unseasonably warm or cold periods can affect yield and quality. These conditions can be avoided by harvesting the commodities earlier than the harvest date, but this may result in poor yield and quality. On the other hand, delayed harvesting also results in a better yield, but increases the risk of weather problems. Thus, proper harvesting timings of horticultural commodities often play an important role.

Assessment of harvesting time is a critical time for growers of fruit and vegetables to decide the accurate period of crop harvesting. Fresh produce is normally ready for harvest when it has developed to the ideal condition for consumption and the condition is usually referred to as harvest maturity. Harvest maturity thus refers to the time when the “fruit” is ready to harvest and the time required to reach market. The harvesting should be done earlier than its ideal maturity for proper marketing.

Most growers decide harvesting time for different commodities by looking and sampling. Judgments are based on colour, size and shape (Sight); texture, hardness or softness (Touch); odour/aroma (Smell); sweetness, sourness, bitterness (Taste) etc. Experience is the best guide for this kind of assessment. Harvest maturity can readily be observed in some fruit crops but it can be more difficult in case of some fruits like avocados remain unripe off the tree after maturity.

1. MATURITY INDICES

Maturity indices (right time of harvesting) are quality factors that give clues as to when a product should be harvested to allow marketing flexibility and ensure the attainment of minimum acceptable quality to the user. It also refers to the set of criteria used to determine the stage of maturity for harvesting the horticultural crops. Commodities harvested either pre-mature or too late are more susceptible to postharvest physiological disorders, diseases and have poor shelf life than harvested at right stage of maturity. The pickers/ handlers are trained in methods of identifying produce that is ready for harvest. However, optimum harvesting stages depends upon type of produce and their final use. Horticultural crops may be harvested at two main maturity levels i.e., physiological maturity and commercial or horticultural maturity. Therefore, the proper stage/maturity indices for harvesting of all horticultural crops should be identified for their implementation during harvesting. Horticultural maturity in relation to developmental stages of the plants is depicted in Fig 3.1.

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

I. INTRODUCTION

Horticultural crops include fruits, vegetables, flowers, plantation crops, spices, and medicinal and aromatic plants have emerged as a major economic activity in India. It contributes 30% GDP from 11.73 % of its arable land area. Horticulture crops cover over 23.4 million hectares of area, which is approximately 12.7% of the total gross cropped area of the country and contributes 18-20% of the gross value of India’s agricultural output (NHB, 2013). India is the world’s second largest fruit and vegetable producing country accounting for 77.7 million metric tonnes of fruit and 159.5m M tonnes of vegetables (NHB, 2013). Besides, about 5.66 million tonnes of loose flowers, spices, mushroom and aromatic plants are also produced. The country produces 41% of world mangoes, 23% banana, 24% cashew nut, 36% green peas, 10% onion and 30% cauliflower and accounts for 12% of world fruit and 13.25% of vegetable production (NHB, 2013). Owing to wide diversity of agro climatic conditions, almost all types of temperate (apple, peach, plum, apricot, cherries and nuts), subtropical (mango, guava, papaya, etc), tropical (banana, pineapple etc), arid fruit (ber and aonla), plantation crops (coconut, cashewnut and arecanut) and diverse variety of vegetables are grown in India. The export of fresh fruit and vegetables from India in 2006-07 was estimated at 9.84 Lakh tonnes valued at Rs 2411.70 crores. Mango, grapes, apple, onion, potato, green vegetables like okra, bitter gourd and green chillies were the major items of export. Similarly, India exported 5.97 Lakh tonnes of processed foods comprising of mango pulp, juices, concentrates, dried and processed vegetables, pickle and chutney, alcoholic and non-alcoholic beverages worth Rs 2818.1 crores (APEDA, 2012) (Table 1 to 3).

Importance

It is one of the most valuable primary products in world trade, in many years second in value only to oil as a source of foreign exchange to producing countries. Its cultivation, processing, trading, transportation and marketing provide employment for hundreds of millions of people worldwide. Coffee is crucial to the economies and politics of many developing countries; for many of the world’s Least Developed Countries, exports of coffee account for more than 50 percent of their foreign exchange earnings. Coffee is a traded commodity on major futures and commodity exchanges, most importantly in London and New York.

Importance

It helps to cope with fever, cough and cold, stress, high blood pressure, excessive fats in body. It lowers the cholesterol level, cleanse the body by eliminating toxic substances, improve the digestive system, improve blood circulation, cleanses other organs of our body including kidney, pancreas, liver, bladder etc.and aid to deal with menstrual problems. The oil is distilled from leaves and flowering tops of Lemon grass. The oil has strong lemon-like odour, due to high percentage (over 75%) of citral in the oil. The characteristic smell of oil makes its use in scenting of soaps, detergents, insect repellent preparations. However, the major use of oil is as a source of citral, which goes in perfumery, cosmetics, beverages and is a starting material for manufacture of ion ones, which produces vitamin – A. The Citral rich oil has germicidal, medicinal and flavouring properties. An allied species called West Indian lemon grass (C.citratus) has low citral content in the oil and has meager trade in the country.

Spices are non-leafy parts (e.g. bud, fruit, seed, bark, rhizome and bulb) of plants used as a flavoring or seasoning, although many can also be used as a herbal medicine. They are those plants, the products of which are made use as food adjuncts to add aroma and flavour or they are derived vegetable substances which are used for seasoning the food imparting distinctive flavour and fragrance that gives zest or piquancy for enjoyment. The term ‘spice’ originated from the Latin word ‘species’, meaning of specific kind. A closely related term, ‘herb’, is used to distinguish plant parts finding the same uses but derived from leafy or soft flowering parts. For example Black pepper, Ginger, Turmeric, Chilli, Cinnamon, Nutmeg etc. Spices are generally composed of fiber, carbohydrate, fat, sugar, protein, gum, ash, volatile (essential oils), and other nonvolatile components. All of these components impart each spice’s particular flavour, colour, nutritional, health, or preservative effects. The flavour components (volatile and nonvolatile) are protected within a matrix of carbohydrate, protein, fiber, and other cell components. When the spice is ground, cut, or crushed, this cell matrix breaks down and releases the volatile components (Raghavan, 2007).

1. INTRODUCTION

Landscaping is a branch of ornamental horticulture having aesthetic and beautifying value which freshens up the atmosphere, enhances the look of the environment and improves beauty of land. In the area of landscaping, it is beneficial to know about ground covers (grasses etc), hedging plants, and focus plants that will complement one another.

A garden by definition is a piece of land devoted to growing flowers, fruits, vegetables, shrubbery and turf. Gardens brighten their surroundings and they add to the beauty of the landscape. The types of flowers that can be grown in a garden depend on the size of the garden, its location and the amount of sunshine and shade it gets.

1. INTRODUCTION AND IMPORTANCE

Flower arrangement is a very old art. In India, the flowers have been earlier arranged in temples and during festivities. The first rules of ikebana were laid down in Japan more than a thousand years ago. Flowers have been widely used for interior decoration, but they were cut from the garden and simply massed into water-filled containers to brighten the home. The beauty of the display relied upon the blooms themselves rather than the aesthetic appeal of the design. Even at homes and venues of festivals/functions/on auspicious occasions, flowers are used in a big way in the form of garlands, wall hangings, and floor decorations. Flower arrangements grace table tops, window sills, corners, fire places, and so on to suit the occasion.

Flower arrangement may be defined as the art of organizing and grouping together plant materials (flowers, foliage, fruits, twigs etc) to achieve harmony of form, colour and texture, thereby adding cheer, life and beauty to the surroundings.

Various types of arrangements are chosen, as appropriate to the area and occasion. Medium sized ‘round’ arrangements are often provided at the guest relations executives’ desk in the lobby and on coffee tables in the lounges. In most five-star hotels, one can see huge, spectacular arrangements in the loggies. Table arrangements for conferences must be low so that guests may see over them. At informal banquets, large arrangements may be seen. At wedding banquets, wall arrangements using gerberas are very popular, now a days. On special occasions and festivals, a large amount of flowers is required for making up various types of arrangements. The extent to which flowers are used in hotel interiors depends on the degree of luxury provided, the number of special functions held.

The housekeeper is responsible for all flower arrangements and their placement. Simple arrangements such as a bud in a vase can be done. Alternatively, flower arrangements may be provided on contract, in which case the arrangements are brought in and taken away at agreed times and little or no floral work is carried out on the premises. Arrangements like running water, containers, buckets, vases, scissors and flower pots are important.

1. INTRODUCTION

Annuals are the group of plants that complete their life cycle in one season or in one year during which they grow, flower and produce seed; and after this they wither out. A true annual (like zinnia) is a plant that will grow from seeds, flower, produce seeds and die in one season. Zinnias provide a succession of flowers for summer colour in any sunny location with good soil drainage

The term annual when applied to herbaceous ornamentals refers to plants that are grown for only one season. A true annual completes its life cycle (bears seed) in one season and has an extended bloom span lasting (in most cases) throughout the summer. Some plants that we use like annuals in the garden actually are herbaceous perennial plants that bloom the first season but are unable to survive the winter climate.

Some varieties will self-sow or naturally reseed themselves. This may be undesirable in many flowers because the parents of this seed are unknown and hybrid characteristics get lost. Plants will scatter everywhere instead of growing in their designated spot. Examples: Alyssum, Petunias and Impatiens. Impatiens flowers are currently the most popular annuals and are unsurpassed for abundant flowers in a shady garden. Some perennials, which are plants that live from year to year are classed with annuals because they are not winter-hardy and must be set out every year. Examples are begonias and snapdragons.

Typically, the annuals we think are as loving the heat of summer, but there are some popular annual plants that prefer the cool of spring and fall. Examples: pansies, snapdragons, ornamental cabbage and kale. These plants actually grow more lush and vigorous when the weather is cool not hot and dry; therefore, they are often offered for sale in early spring.

1. INTRODUCTION

Medicinal plants constitute considerably a large component of natural vegetation. Several of these species are in great demand for domestic consumption as well as for commercial use by the herbal industry. About 800 species are estimated to be in trade with a turnover of Rs.4000 crores per year. This high demand by the herbal industry has put enormous pressure on the wild populations leading to destructive collection of the produce as maximum species of medicinal plants are wild.

A medicinal plant is as any plant which contains substances that can be used for therapeutic purposes or which are precursor for chemo- pharmaceutical synthesis. The medicinal plants are useful as a drug or therapeutic agent, having different medicinal values in form of active ingredient. Medicinal plants may therefore be defined as a group of plants that possess some special properties or virtues which make them important as drugs, therapeutic agents and for medicinal purposes.

Medicines from India are used by about 60 per cent of the world’s population. These are not only used for primary health care not just in rural areas in developing countries, but also in developed countries as well where modern medicines are predominantly used. While the traditional medicines are derived from medicinal plants, the herbal drugs are prepared from medicinal plants only. Use of plants as a source of medicine has been an ancient practice and is an important component of the health care system in India. There are about 45,000 medicinal plant species in India, with concentrated spots in the region of Eastern Himalayas, Western Himalayas, Western Ghats and Andaman & Nicobar Island. India is the largest producer of medicinal herbs and is called the botanical garden of the world. In rural India, 70 per cent of the population depends on the traditional type of medicine, the Ayurveda. Ayurveda and Kabiraji (herbal medicine) are two important forms of alternative medicine that is widely available in India. It is estimated that about 80,000 species of plants are utilized in some form or other by the different systems of Indian medicine. Many herbs and spices are used in Indian cooking, such as onion, garlic, ginger, turmeric, clove, cardamom, cinnamom, cumin, coriander, fenugreek, fennel, ajowan (ajwain), anise, amchur, bay leaf, hing (asafoetida) etc. Ayurvedic medicine uses all of these either in diet or as a medicine.