eChapters

49.1. Aim

To perform practical on drying/dehydration of different vegetables.

49.2. Theory

Drying refers to the method of removal of moisture content from the food to a level at which the activities of food spoilage and food poisoning micro-organism are inhibited. Therefore, reduction in water activity of the food is the main principal of preservation by drying. In the dehydrator initial temperature is generally kept at 43oC which is then gradually increased to 60-66oC for vegetables. The product shall be prepared from wholesome vegetables free from blight, insect infestation and fungal discoloration. Only edible portion of the vegetables shall be used and it shall be free from stalks, peels, stems, and extraneous matter. The dried/dehydrated vegetable may contain permitted preservative. The finished product shall be of good edible quality and shall reasonably reconstitute to its original shape and quality on boiling for 15 minutes to an hour. The finished product shall be free from visible mould, insect or larvae. Kind of dry vegetable shall be declared on the label. Acid insoluble ash shall not be more than 0.5%.

Demand-driven vegetable breeding emphasizes the development of new cultivars based on specific consumer preferences, market demands, and environmental conditions. This approach contrasts with traditional breeding methods that primarily focus on agronomic traits like yield or disease resistance. The importance of demand-driven breeding lies in its ability to align agricultural production with consumer needs and market trends. By directly responding to consumer preferences such as taste, appearance, nutritional content, and culinary characteristics, breeders can create vegetables that are not only commercially viable but also meet consumer expectations. This approach enhances market acceptance and profitability for growers, distributors, and retailers alike. Breeders prioritize demand-driven breeding to stay competitive in a rapidly evolving market where consumer preferences can shift quickly. By understanding and responding to these preferences, breeders can develop cultivars that offer distinct advantages over existing varieties, leading to increased market share and improved economic outcomes for stakeholders throughout the supply chain. This chapter provides an understanding on demand-driven vegetable breeding, particularly opportunities, and challenges.

Sustainable development and efficient management of water is an increasingly complex challenge in India. Increasing population, growing urbanization and rapid industrialization combined with the need for raising agricultural production generates competing claims for water. Ground water has an important role in meeting the water requirements of agriculture, industrial and domestic sectors in India. About 85 percent of India’s rural domestic water requirements, 50 percent of its urban water requirements and more than 50 percent of its irrigation requirements are being met from ground water resources. Ground water is annually replenishable resource but its availability is non-uniform in space and time. Technically, the dynamic ground water refers to the quantity of ground water available in the zone of water level fluctuation, which is replenished annually. Hence, the sustainable development of ground water resources warrants precise quantitative assessment based on reasonably valid scientific principles. National Water Policy, 2012 has laid emphasis on periodic assessment of ground water resources on scientific basis. The trends in water availability due to various factors including climate change must also be assessed and accounted for during water resources planning. To meet the increasing demands of water, it advocates direct use of rainfall, desalination and avoidance of inadvertent evapo-transpiration for augmenting utilizable water resources. The National Water Policy, 2012 also states that safe water for drinking and sanitation should be considered as pre-emptive needs followed by high priority allocation for other domestic needs (including needs of animals), achieving food security, supporting sustenance agriculture and minimum eco-system needs. Available water, after meeting the above needs should be allocated in a manner to promote its conservation and efficient use.

1. Introduction

The Indian economy is heavily dependent on agriculture (employing 53 % population). Around 84 % of the holdings are less than 2 ha while approximately 67% of the farms have less than 1 ha of operational farmland (Government of India, 2013; Pandey and Suganthi, 2015). A large fraction of the farming population is mostly reliant on monsoon rains for irrigation. The condition of the farmers is found to be deteriorating year after year with the revelation that 27% of farmers did not consider farming as a profitable enterprise and 40% were ready to migrate to other avenues for employment (NSSO, 2005; Pandey and Suganthi, 2015). 

Individuals suffering from the more pronounced types of mental disorder are sometimes said to be insane, but the term insanity is a legal one, not a medical term. An insane person is one who cannot distinguish right from wrong or assume responsibilities for his own actions. In the case of a psychotic patient the whole personality is involved. And he is completely out of touch with real world around. Not so the neurotic patient. Only a small part of his personality is involved. Psychotic patients often require hospitalization; neurotic patients rarely do, unless they have some additional physical problem.

Another distinction between these two major groups is that his friends usually bring the psychotic patient to the doctor, while the neurotic comes of his own accord. He knows he is sick, and he usually wants help. Most often he is suffering from a nervous depression.

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

Isolation of Peripheral Blood Lymphocytes

Chemicals

1. Heparin

2. Sterile PBS (pH 7.2)

3. Histopaque-1077

4. RPMI-1640 Media

Protocol

Collect 5 ml of blood from the animal in 1 ml (20 IU/ml of blood) of diluted heparin and add 2 ml of PBS. Layer over the diluted blood on 5 ml of histopaque-1077 in 15 ml centrifuge tube and centrifuge at 1500 rpm for 40 min. Collect the buffy coat at the interface of plasma and histopaque in separate centrifuge tubes. Wash the cells thrice with PBS and suspended in 2 ml of RPMI-1640 media supplemented with 10% fetal calf serum. Determine the percentage cell viability by 0.1% trypan blue exclusion test, using hemocytometer. Finally dilute the cell suspension to 106 cells/ml in RPMI 1640 medium.

Injudicious and indiscriminate use of several agrochemicals viz. pesticides, herbicides, insecticides, dyes and medicaments in the field of agriculture and veterinary practices threatens the human and animal population for the toxicity of these environmental deterrents. These agrochemicals have been associated with several episodes of accidental poisoning and mortality among human and animal population. The episode of Mass ethion poisoning in Gujarat is one of the commonest example of inclusion of pesticides or agrochemicals in the environmental pollutants. There are modern tools to evaluate and analyze these chemicals in the environmental pollutants of human and animal use. The sensitivity of these modern highly sophisticated tools has now immensely changed the analytical environmental pollutants industry. Gas chromatography and Mass Spectrophotometry i.e. (GC-MS) technique is employed to detect the volatile residues from environmental pollutants. Whereas, High performance liquid chromatography i.e. HPLC is employed to analyse and detect non volatile drug residues. Gas Chromatography/Mass Spectrometry (GC/MS) instrument separates chemical mixtures (the GC component) and identifies the components at a molecular level (the MS component). It is one of the most accurate tools for analyzing environmental samples.

ABSTRACT

Genetic relationships were estimated among twenty five ashgourd (Benincasa hispida (Thunb.) Cogn.) landraces of different types using PCR-based random amplified polymorphic DNA (RAPD) markers. The molecular survey of the ashgourd germplasm by RAPD using three random primers resulted in twenty scorable bands (average of 6.66 bands per primer) of which two were monomorphic and rest, 18 were polymorphic (90.0%). Pair-wise genetic similarities among the landraces determined using Jaccard’s coefficient ranged from 0.14 to 1.00. The dendrogram based on clustering matrix separated the landraces with morphologically distinct smooth textured fruits from waxy textured fruits and the former group showed less divergence than the latter. With in the group of waxy textured fruits, limited variation was detected among landraces with small and medium sized fruits. Further, morphologically similar landraces with large fruits form distinct clusters with in the major clusters. The study demonstrated the usefulness of the RAPD marker analysis in effectively assessing the genetic relationship present among the ashgourd landraces examined.

ABSTRACT

Genetic variability was assessed among twenty five distinct drumstick (Moringa oleifera Lam.) landraces using PCR based random amplified polymorphic DNA (RAPD) markers. Genomic DNA extracted from tender leaves was used for RAPD analysis. Thirty five primers were screened and based on the number of bands three primers were selected. The primers produced 34 bands, of which 58 % (20 bands) were polymorphic. The data were analysed using NTSYS pc 2.1 software. The similarity matrix constructed using Jaccard’s coefficient method was subjected to cluster analysis using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) and dendrogram was generated. The pair wise coefficient values in the genetic similarity matrix among the landraces varied between 0.28 and 1.00. The dendrogram based on the matrix separated the landraces with distinct morphological characters and it was noted that the cluster formation was not fully in agreement with the geographical location. The study showed the use of RAPD markers in detecting genetic variability present among the drumstick landraces studied.

55.1. Aim

To determine dietary fibres in given samples.

55.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre.

55.3. Procedure for estimation of dietary fibre

55.3.1. Acid detergent fibre (ADF)

Reagents: Cetyl tri-methyl ammonium bromide (CTAB) reagent and Acetone

Apparatus: Digestion apparatus vacuum pump, oven, desiccator and sintered glass crucible.

Purpose: The organic acids present in fruits and vegetables influence the flavour, colour, keeping quality and maturity. The maturity standards are determined by the acid and sugar ratio. The total acidity (citric acid) is determined by titrating the juice against standard alkali solution.

Equipments and chemicals: 10 ml., pipette, 25ml. Burette, small glass funnel, dropper, juice extractor, distilled water, 100ml. volumetric flask, burette stand, beaker, N/10 sodium hydroxide, filter paper, phenolphthalein indicator (one gram of phenolphthalein in 100ml. of 95% ethyl alcohol)

High-performance liquid chromatography or high-pressure liquid chromato graphy (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture. It involves the injection of a small volume of liquid sample into a tube packed with tiny particles (1.8- to 5-micron (μm) particle size called the stationary phase) where individual components of the sample are moved down the packed tube (column) with a liquid (mobile phase) forced through the column by high pressure delivered by a pump. These components are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles. These separated components are detected at the exit of this tube (column) by a flow-through device (detector) that measures their amount. An output from this detector is called a “liquid chromatogram”.

Aflatoxins are toxic and carcinogenic compounds produced as metabolites by many Aspergillus species. This fungus invades habitats such as soil, grain, nuts, or decaying vegetation when- ever suitable conditions such as high moisture and temperature are met. B1 is the most toxic of at least 14 different types of naturally occurring aflatoxins. Therefore, many countries and regulatory agencies impose strict limits on B1 as well as other common aflatoxins such as B2, G1, and G2 despite exhibiting lower toxicity. The U.S. FDA set the action level to20 μg/kg (ppb) for human food and to 300 μg/kg (ppb) for animal feed. Aflatoxin B1 is extremely widespread and is present in food and feed products such as cottonseed, corn, and peanuts. It is highly toxic and has been classified as a group 1 carcinogen by the WHO. The aflatoxins B2 , G1 , and G2 are usually found accompanying B1 , in lower concentrations in the contaminated samples. The order of toxicity is AFB1 > AFG1 > AFB2> AFG2.

Principle: Sample extract in oxalic acid is titrated against standard sodium 2, 6 dichlorophenolindophenol dye to a faint pink colour which persists for 5-10 seconds.

(Indophenols Method)

Purpose: The ash content of plants represents the deposition of minerals elements in the plant or in its parts. The value of ash content in the plant tissue indicates the health of the plant. Plant ash is also used both in qualitative and quantitative estimation of mineral elements.

Material and Equipments: Crucible, oven, chemical balance and weight box.

54.1. Aim

To determine crude fibre in given samples.

54.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre (Cellulose + lignin + hemicelluloses + pectic substances). Crude fibre is the organic residue of a sample. A fat free sample is treated with boiling sulphuric acid and subsequently with sodium hydroxide. The residue after subtraction of ash is regarded as crude fibre.

54.3. Reagents/ Apparatus/Glassware

1. 0.255 N Sulphuric acid solution

2. 0.313 N Sodium hydroxide solution

3. 10% Potassium sulphate solution

4. Ethanol

Fructose, a keto-hexose (called as fruit sugar), is usually accompanied by sucrose in fruits and estimated by the methods of Ashwell (1957).

Materials

Resourcinol reagent: Dissolve 1g resorcinol and 0.25 g thiourea in 10 ml glacial acetic acid. This is stable in the dark.

Dilute HCl: Mix five part of conc. HCl with one part of distilled water.

Standard fructose solution: Dissolve 50mg of fructose in 50 ml water. Dilute 5 ml of this stock soln. to 50 ml for a working standard.

Preparation of the material: Common minute material for analysis in Waring blender for 1 to 2 minutes. Do not over heat. Pass it through No.60 sieve and put is in airtight container.

Procedure: Take 2 to 3 g sample in duplicate in pre-dried and pre-weighed 7 cm diameter aluminium dishes. Remove the dish cover and put them in vacuum oven and dry for 6 hours at 88°C and 26-22 inch vacuum. During this process, admit to oven a slow current of air (2 bubbles per second) dried by passing H₂SO₄ replace the cover, cool it in desiccators and weigh again.