eChapters

7.1. Origin and distribution

• Native of South East Asia, Central America.

• Mexico - largest producer of diosgenin.

• In India, it is grown in North eastern states and Goa

• Dioscorea alata and Dioscorea esculenta - edible tubers.

• Dioscorea floribunda, Dioscorea villosa, Dioscorea composita - widely grown

• Major producing countries - Mexico, Guatamala, Costa Rica, India and China.

Introduction

Acute lung injury (ALI) is the manifestation of an inflammatory response of lung resulting from severe sepsis, bacterial or viral pneumonia, trauma, and burns in animals (Ali et al., 2020).It is distinguwashed by disruption to the alveolar capillary membranes, atelectasis of the pulmonary airspaces, and neutrophil and protein-rich fluid infiltration into the alveolar space (Li et al., 2019). Although advances in treatment, the disability and fatality rates of ALI remain high, that is around 30-40% (Ali et al., 2020). At the moment, new therapeutic alternatives are urgently required to treat ALI. As a result, this is a very active area of research to understand the molecular principles underlying pulmonary inflammation and ALI (Reiss et al., 2012). The use of animal models of inflammatory lung has allowed for the probing of pathways of inflammation responsible for injury. Specially, rodent models may serve a link between in vitro research to large animals and clinical studies by allowing for high quantities of samples under physiological circumstances at low expenses (Domscheit et al., 2020).LPS, found in the outer membrane of gram-negative bacteria, consists of a polar lipid head group (lipid A) and a chain of repeating disaccharides (Gao et al., 2023). LPS induced lung injury model, closely mimic sepsis and inflammatory lung diseases, is a well-establwashed model in this field of research (Reiss et al., 2012). To mimic acute lung injury, LPS can be administered either directly through intratracheal or intranasal route (direct pulmonary insult) or indirectly through intraperitoneally and intravenous routes (indirect extra pulmonary insult) (Perl et al., 2011). This chapter focuses mostly on rodent model of acute lung injury caused by lipopolysaccharide (LPS), both direct and indirect. Additionally, it also includes the characteristics and underlying molecular mechanism of LPS induced lung injury along with applications in drug development.

ABSTRACT

In vitro propagation of Plumbago rosea was attempted. One year old plants were used as the source of explants. Nodal explants were inoculated on half strength MS medium supplemented with 2,4-D 1.0 mg l^-1. Induction of callus was observed three weeks after inoculation. The calli were sub-cultured on half strength MS medium supplemented with BA (0.5, 2.0, 3.0 and 4.0 mg l^-1) and IAA (0.0 and 1.0 mg l^-1). Eight treatments with six replications were tried. Benzyl adenine 2.0 mg l^-1 promoted the proliferation of shoots (23.2). Rooting of micro shoots was obtained on MS medium supplemented with IBA 1.5 mg l^-1. The plantlets were then transferred to pots containing sand/soil mixture (1:1) and kept in a mist chamber. The survival rate was found to be 90 per cent.

1. DISEASES OF MANGO

Mango is considered to be the king of fruit. India is the largest producer and exporter of mango in the world. Mango possess unique nutritional and medicinal qualities apart from being a rich source of vitamins A & C, besides its attractive form and appearance, delicious taste and appetizing flavor, the ripe mango fruit according to nutritional experts is also highly invigorating, fattening, laxative and diuretic. Every part of mango from root to tip is used in a variety of ways. This crop is affected by many fungal, bacterial and other non parasitic diseases.

1. Mango Malformation Disease (MMD)

Mango Malformation Disease is a fungal disease of mango caused by several species of Fusarium, some yet to be described. Mango is the only known host of the disease. The disease spreads on a tree very slowly, but if left unchecked, can severely reduce yields. The main method of spreading MMD to new areas is through infected vegetative planting material. There is no evidence that the disease can spread on fruit or the seeds, or that it affects human health. It usually associated with the bud mite, Aceria mangiferae but the mites have been shown to spread the disease within a tree and not between the trees. Mango malformation, also known as bunchy top, is a very serious threat to the mango industry, particularly in northern India. The Etiology of the disease still remains obscure and diverse claims have been made about its causes, e.g., physiological, viral, fungal, acarological and nutritional.

Importance of fruits in human diet is well recognized since time immemorial. They are important part of a healthy diet as natural sources of energy, essential vitamins, minerals and antioxidants ever since the beginning of humancivilization. Our country is bestowed with varied climatic conditions, which had made it possible to grow diverse group of fruits from its temperate to tropical, to subtropical and even to arid niches. The cultivation of fruit crops is, presently, one of the most important and profitable proposition. Growing fruit crops translate into realization of more yield/ income from a unit area of land than any of the agronomic crops. However, there is need to considerably increase the yield of fruit crops, particularly under changing environmental conditions as under such conditions diseases are getting more prevalent. Therefore by adoption of proper protection practices, yield of fruit crops can be improved.

Plant diseases caused by fungi, bacteria, viruses and phytoplasmas reduce crop yields (Khoury and Makkouk, 2010).Adoption and support for using participatory approaches help to farmers in disease management of fruit crops, reducing costs and improving production efficiency. Fungal, bacterial and viral diseases pose serious threat in the cultivation of fruit crops in India. To reduce the losses caused by plant disease, a complete knowledge of disease management strategies of fruit crops are essential which are as follows:

Introduction
Vegetable crops are highly susceptible to biotic stresses indicated by disease causing micro-organism (fungi, bacteria, virus and phytoplasma). Indiscriminate use of chemicals for the control of diseases, insect pest have
several disadvantages, particularly increased cost of cultivation, residual toxicity in food chain and development of resistance against pathogen. Management of diseases involves use of resistant varieties and integration of cultural and chemical control measures. Of the biotic stresses much of the breeding efforts have been directed towards developing resistance against diseases. In fact, breeding for resistance, has been one of the most important objective of vegetable breeder in the last half of the century. Genes that contribute to pathogen tolerance/resistance can be obtained from local germplasm resources, or through exotic lines, wild species or genera, or lines from other breedingprograms. In the biotechnological approaches for vegetable breeding also,  most of the achievement has been to generate transgenic plants resistance to disease. Often breeding for resistance has assumed greater importance in a number of vegetable crops.
Major Diseases of Tomato
Early blight: The casual organism is Alternaria solani. The symptoms appears as small, black spots with enlarged and concentric rings in a bull’s eye pattern in the center of the leaves. Tissue surrounding the spots may turn yellow. On fruits, dark brown concentric rings are seen that affects the market quality.

Introduction

The apple (Malus domestica), a valuable and economically significant crop, is grown in India’s northwestern Himalayan region. After Orange, Banana, and Grape, it is the fourth most widely grown fruit worldwide. The tree is native to Central Asia, where its wild ancestor Malus sieversii is still found today. Apples have been cultivated in Asia and Europe for thousands of years. The world’s largest apple grower is China and in India it is grown in the hills of Uttarakhand, Himachal Pradesh, and Jammu & Kashmir in India. The average summer temperature during the active period is about 21-24°C growing season. Apple does this best in areas where trees are dormant. The color development will be better in winter when plenty of sunlight hits. It is possible even at an altitude of 1500 -2700 meters from sea level. 1000-1250 mm well distributed rainfall during the growing season which is most suitable for optimal growth and fertility of apple trees.

Scab

Ventruia inaequalis (Spilocaea pomi Fr?)

Diagnostic Symptoms

• Symptoms generally noticed on leaves and fruits.

Introduction

The banana (Musa parasidiaca) belongs to the Monocotyledons class, the Zingiberales order, and the Musaceae family. It is indigenous to Southeast Asia. They thrive in tropical and subtropical climates and are widely grown in countries such as India, China, the Philippines, Ecuador, Brazil, Uganda, Colombia, and Indonesia. A banana’s average length is 7 to 9 inches, and its diameter is 2 to 3 inches. Bananas are frequently planted during the rainy or monsoon season, when the soil is moist and the young plants can absorb enough water. In tropical and subtropical climates where they are commonly grown, the planting season typically runs from April to June. Planting may take place in September or October in areas with a dry season, following the rainy season.

Introduction The betel vine, scientifically identified as Piper betle L., belongs to the Piperaceae family, which encompasses plants such as pepper and kava. Paan leaves, widely cultivated in countries like Thailand, Philippines, India, Malaysia, Taiwan, Sri Lanka, and various Southeast Asian nations, are commonly used as a popular mouth freshener after meals. This traditional practice is predominantly observed in South Asia and among specific Asian diaspora communities worldwide, where betel leaves are commonly chewed as part of a betel quid or paan, often combined with areca nut or tobacco.

In the cultural traditions of Odisha, offering a bundle of betel leaves symbolizes respect and marks the beginning of auspicious events. Taxonomically, betelvine falls under the genus Piper in the Division Magnoliophyta, Class Magnoliopsida, Order Piperales, and Family Piperaceae.

Introduction

Brinjal (Solanum melongena), is a self-pollinated plant also known as eggplant, is an easily cultivated plant belonging to the Solanaceae family.Brinjal ishigh productive vegetable and easily grown everywhere.It is also known as poor’s man vegetable. Brinjal is cultivated widelyand grown as a vegetable crop in any environment. Brinjal grown large amount in India, Pakistan, Chinaand United States. The origin of the brinjal is India. In Brinjaleconomic parts are fruit. It is a high nutritional valuable food.The brinjal fruit and whole plant use as a medicine in some area. It is cultivated everypart of India except high attitude, all the year around. Depending upon consumer’s preference and yield, different types of cultivars are cultivated.Somewild spp of brinjal are fond in northeast states of India and Malaysia. Solanum insanum, a wild cultivar of the plant, mainlyobserved in the dry and hilly region of west Bengal.Some yellow color brinjal varieties are found in Malaya.

1. Introduction

Eggplant or brinjal (Solanum melongena L. family. Solanaceae) is the major vegetable crop of wet and hot climatic zone of south Asia (Srinivasan and Huang, 2008). After China, India is the second largest brinjal producer country in the world (Majumdar and Das, 2020). It is nutritious vegetable and consumed in throughout the world. Brinjal is considered as Indian native crop and it has been cultivated for last 4000 year. India is known to be the Origin Centre of brinjal cultivation, from where it distributed to throughout the world (Choudhury and Malda, 1968). In India, brijnal crop grow almost throughout the year. Brinjal fruit contains moisture 92.7g, carbohydrates 4.0g, proteins 1.4g, minerals 0.3g, vitamins 124 I.U. and vitamin 12.0mg per 100 g of edible part (Kalra et al., 1988). It is used as vegetable purpose as well as pickle (Singh et al., 1963).

RICE (Oryza sativa)
Fungal diseases
1. Blast
Symptoms: All above ground parts (leaf, leaf sheath, culm) of the plant are affected. There are three type of blasts (leaf blast, node blast, neck blast)
Leaf blast: Eye or spindle shaped spots on leaves (margin is dark brown and centre becomes ashy) and leaf sheath are observed. Several spots appear on the leaves cause blightening.

Introduction

Chili peppers, also spelled chile or chilli, are varieties of the berry-fruit of plants from the genus Capsicum, which are members of the nightshade family Solanaceae, cultivated for their pungency. Chili peppers are widely used in many cuisines as a spice to add "heat" to dishes.

Botanical Name : Capsicum annum

Family : Solanaceae

Chromosome Number (2n) : 24

Diseases of Chilli

 1. Powdery Mildew

The disease was first reported from Morocco in 1937.

Anthracnose or Fruit Rot and Die Back

Colletotrichum capsici

Diagnostic Symptoms

Dieback symptoms

• Small, circular to irregular, brownish black scattered spots appear on leaves .

1. Introduction

Chirata (Swertia chirayita) belongs to the family Gentiniaceae, a potential medicinal plant used as bitter tonic in treatment of fever and for curing various skin diseases, is a perennial herb and distributed throughout the eastern Himalayan region of India. The plant is a native of temperate Himalayas, found at an altitude of 1200-3000 m (4000-10000 ft), from Kashmir to Bhutan and in the Khasi hills at 1200-1500 m (4000 to 5000 ft). It can be grown in sub-temperate regions between 1500 and 2100 m altitudes. It is used in traditional medicine to treat numerous ailments such as liver disorders, malaria, and diabetes and are reported to have a wide spectrum of pharmacological properties. Its medicinal usage is well-documented in Indian pharmaceutical codex, the British, and the American pharmacopeia’s and in different traditional medicine such as the Ayurveda, Unani, Siddha, Horopathy and other conventional medical systems. This ethno medicinal herb is known mostly for its bitter taste caused by the presence of different bioactive compounds that are directly associated with welfare of human health. The increasing high usage of Swertia chirayita, mostly whole plant, as well as the illegal overharvesting combined with habitat destruction resulted in a drastic reduction of its populations and has brought this plant to the verge of extinction. The increasing national and international demand for Swertia chirayita has led to unscrupulous collection from the wild and adulteration of supplies. The bitterness, antihelmintic, hypoglycemic and antipyretic properties are attributed to amarogentin (most bitter compound isolated till date) 1, swerchirin, swertiamarin and other active principles of the herb. Due to the high demand, this plant is being cultivated in many places of Darjeeling district both for domestic use within India and for exporting purpose. During our roving survey (2011-2014), it was observed that this high value medicinal plant facing a number of diseases (like seedling blight, leaf spot, Leaf blight etc.), ultimately, cultivation of Swertia chirayita is getting hampered. The quality of plant is affected due to the occurrences of diseases as whole plants part being used as medicinal purpose and also affects the marketing scenario; the demand of good quality chirata is increasing at a rate of 10% annually. To harvest the benefit of megabiodiversity zone of eastern Himalaya needs urgent attention to combat the upcoming epidemic of diseases of chirata. Here, we are trying to throw some light related to present status of diseases of chirata and standard management practices.

Introduction

Chrysanthemum, commonly known as “mums” or “chrysanths,” is a genus of plants that bloom and belong to the Asteraceae family. These plants are native to Asia and northeastern Europe, with China being the primary centre of diversity for the genus. Chrysanthemums are well-known for their attractive and colorful f lowers, and they are popular ornamental plants in gardens, floral arrangements, and as cut flowers.

Chrysanthemum morifolium is a herbaceous perennial flowering crop. It is the second most popular cut flower worldwide, right behind roses, and a significant decorative plant for commerce (Silva et. al., 2013). Certain species’ flowers, such as those of Chrysanthemum morifolium and Chrysanthemum indicum, are rich in nutrients and biologically active substances and are used to make medicinal tea and cosmetics, respectively (Lin et. al., 2010).

Gummosis

Phytophthora spp

Diagnostic Symptoms

• Dark water soaked large patches appear on the basal portions of the stem near the ground level.

Introduction

The innermost layer of the pericarp is the endocarp. The segments are also called “liths”, and the space inside each lith is a locule filled with juice vesicles, or “pulp”. From the endocarp, stringlike “hairs” extend into the locules, which provide nourishment to the fruit as it develops. Citrus fruits are notable for their fragrance, partly due to flavonoids and limonoids (which in turn are terpenes) contained in the rind, and most are juice-laden. The juice contains a high quantity of citric acid giving them their characteristic sharp flavour. The genus is commercially important as many species are cultivated for their fruit which is eaten fresh, pressed for juiceor preserved in marmalades and pickles.

Coconut

 Kingdom: Plantae

 Phylum: Magnoliophyta

 Class: Liliopsida

 Subclass: Arecidae

 Order: Arecales

 Family: Arecaceae

 Genus: Cocos

 Species: Cocos nucifera Linnaeus

 1. Bud Rot

The fungus Phytophthora palmivora is responsible for the ailment known as “bud rot.” Butler first mentioned bud rot in India in 1906. Due to a number of factors, the disease has recently spread to practically every region of the state in a very serious manner.