eChapters

Purpose-Multiplication of plants by cutting includes stem, root and leaf cuttings. The stem cuttings are of four different types i.e. hard, semi hard, soft wood and herbaceous wood cutting consist in producing new individuals after the piece of stem has been detached from the mother plant and planted in conditions suitable for regeneration to the missing organs. Stem cutting have to develop a new root system as they already have buds to give rise to shoots. Many cells of the cutting are capable of becoming meristematic and thus are able to produce new roots. The success in the stem cutting multiplication depends upon factors such as condition of the mother plants, part of the tree from where the cuttings are made, time of the year, care while planting and after care. Cuttings can be made during monsoon (July-Sept.) and spring (Feb. -March) season when active cell division is taking place.

Purpose: In propagation of fruit plants by any vegetative methods such as cuttings, budding and in to the selection of ‘mother plants” and “bud-wood”. Use of bud-wood or any other propagation material from high yielding, true to-type, disease free trees (mother plant) will ensure uniform behaviour of trees in the orchard. If possible, propagation material from a single source is used.

Bud-wood used for budding purpose from high yielding, true-to-type and disease free trees certified for the above character by a certifying agency is referred as ‘certified bud’

Procedure: By adopting the flow sheet given below, clean scion from healthy, productive plants, can be selected for vegetative propagation.

Purpose: It is important that the plants occupy the same position where the pegs were placed in the field. At the time of planting the tree the peg is removed first followed by pit making to receive the plant. The plant may be with the ball of earth or without the ball of earth. While planting the tree in the pit there is every possibility that plant may not occupy the same position where the pegs were originally placed. In order to overcome this problem planting board is used. Use of a planting board ensures the planting of the plant in the correct position.

Materials and equipments: Spade, khurpi, planting board, wooden pegs and chain.

Problem- Prepare a layout plan for establishing on ideal mango orchard near Varanasi city in 2ha area under high density planting. Calculate the number of plants needed for planting and amount of DAP and sulphate of potash heeded or planting. Also mention the name of cultivars for this region under HDP (200:100:200 NPK g/plant)

Problem-Prepare of a layout plan of orchard for 10 ha. area which is situated on the highway. The dimension of the orchard is 3:2. Calculate the areas under each crop which will you grow and also write their suitable variety along with their suitable diagram. Also fulfill the following information about orchard

Purpose-There are several planting plans or systems which can be adopted for planting orchard. The following points need to be considered before choosing any system of planting.

1. It should accommodate maximum number of plants per unit area.
2. It should allow sufficient space for the development of each tree.
3. Orchard operation such as ploughing, dusting are easy to perform.

Purpose- Burnt porous clay or cement or plastic pots are commonly used for various purposes in horticulture. The pots are used in propagation or plants, cultivation of ornamental plants and flowering annuals. Growing of plants in pots in referred as pot-culture. In pot-culture, operations such as potting, shifting and filling of pots are very important. Potting refers to planting of plants in pots containing soil mixture, while shifting means removal of plants from pots and planting them again in the same or different pot. Plants growing for more than one year in the same pot need shifting. The time for potting and shifting of plants is monsoon season (July to Sept.) and during spring (Feb-March).

Materials and Equipments-Flower pots, crocks (broken pieces of pots), potting mixture, khurpi and watering can (with fine nozzle).

Sickle: Used for cutting grass, fodder and harvesting crop. Sickle made of steel is better and lasts longer.

A. Research journal

1. Haryana Journal of Horticultural Science (Quarterly)-Society of Haryana, Department of Vegetable Crops, CCS, HAU, Hisar (Haryana).
2. Hort. Science (Annualy)-American Society for Horticultural Sciences 600, Camerun Street, Alexandria, USA
3. Horticulture Research (Half yearly)-ScottishAcademic Press, 25, Perth Street, Edinburgh.
4. Indian Journal of Horticulture (Quarterly)-Horticulture Society of India, Division of Fruits and Horticultural Technology, IARI, Pusa, (New Delhi).
5. Journal of Applied Horticulture (Half yearly)-Managing Editor, Journal of Applied Horticulture, A-859, Indiranagar, Lucknow (UP).
6. Progressive Horticulture (Quarterly)- Hill Horticulture Development Board, Horticultural Experiment and Training Centre, Chaubattia, Ranikhet, (Uttarakhand).
7. Scientia Horticulture (Annualy)-Elsevier Science P.O. Box 945, New York,

A. ICAR Agricultural Institutes

• Central Agricultural Research Institute (CARI), Post Box No. 181. Andaman Nicobar & Lakshwadeep Group of Islands, Port Blair.
• Central Arid Zone Research Institute (CAZRI), Jodhpur, (Rajasthan). Website: www.icar.org.in (Rajasthan).
• Central Avian Research Institute (CARI), Izatnagar, (Uttar Pradesh)
• Central Inland Fisheries Research Institute (CIFRI), Barrackpore (West Bengal). Web site: www.cifri.com.
• Central Institute for Cotton Research (CICR), P.O. No. 225, G.P.O., Panjari, Wardha Road, Nagpur (Maharashtra).
• Central Institute for Freshwater Aquaculture (CIFA), P.O. Kausalyaganga, Bhubaneshwar (Orissa).

1. Agriculture universities in India

A. State agriculture universities in India

1. Acharya NG Ranga Agricultural University, Guntur, Hyderabad, Telangana
2. Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh
3. Assam Agricultural University, Jorhat, Assam
4. Bihar Agricultural University, Sabour, Bhagalpur, Bihar
5. Bihar Animal SciencesUniversity, Patna, Bihar

Introduction

“The World Packaging Organisation’s (WPO) slogan, “Better Quality of Life Through Better Packaging”, sumps up the important place that packaging occupies in a modern economy.

As the economics of many countries are increasing, the consumers have started using more processed food. As a matter of fact global food processing and packaging business has reached to multi trillion dollars. In today’s world of global markets and stiff competition in every product along with increasing consumer demand, it becomes imperative for companies to explore ways to improve their productivity in terms of maintaining safety, using sustainable packaging materials, implementing flexible and standardized technology, and adopting proven management. Towards this end, companies have been striving to improve the efficiencies of their operations through improvements in the processes. Reducing wastes and shortening lead times becomes all the more imperative in the food industry due to the perishable nature of the product. Moreover, recent advances in technology such as Radio Frequency Identification (RFID) and nano-materials afford companies greater opportunities to streamline the processing, packaging, and manufacturing schemes.

Introduction

Microencapsulation is a process of entrapping solid particles, liquid droplets or gases in thin polymeric coatings. The outer film (wall) protects the encapsulated material (core) and allows for its controlled release. Application of this technique is wide spread in several industrial fields, including the manufacture of foods, pharmaceuticals, cosmetics and pesticides. Common food ingredients subjected to microencapsulation include probiotic bacteria, acidulates, flavours, colours, sweeteners, minerals and vitamins. A number of coating materials are employed in production of microcapsules, such as gums, starch, and starch derivatives, cellulosic materials, lipids, proteins and inorganic materials.

Actually the development of the formation of micro-encapsulates was partially inspired by the nature. Eggs, seeds, grains, nuts, fruits etc. are typical examples of the food encapsulation and natures own way of conserving and protecting natural products against its own deteriorating elements. Taking clue from this, man adopted this technology of protection of some of the foodstuffs as sauces, puddings etc. Microencapsulation can improve the convenience of food. The shell provides a barrier between reactive components (for instance, delaying the release of leavening agents for fluffier bread products or protecting oxygen-sensitive materials during processing and storage). Microcapsules can help fragile and sensitive materials survive processing and packaging conditions and stabilize the shelf-life of the active ingredient.Karel (1994) described various methods of encapsulation of food ingredients.Inaddition, microencapsulation can simplify the food manufacturing process by converting liquids to solid powder, decreasing production costs by allowing batch processing using low-cost, powder-handling equipment. To improve food safety, the technology can be used to indicate product tampering, thermal spoilage, and freeze-thaw cycles.

Preservation methods are generally believed to be responsible for a depletion of naturally occurring antioxidants in food. Processed fruit and vegetables are expected to have a lower health protecting capacity than fresh ones. This is because, up to now, only selected and barely stable antioxidants of nutritional interest (e.g. ascorbic acid) have been commonly assessed as indicators of processing damage. On the basis of these supposed negative effects, in the past few years, the main tool for minimizing eventual processing damage was the strategy of ‘reconstitution’, achieved by the addition or the enrichment of the product with natural antioxidants.

It is well known that food processing can have many effects not all of which result in a loss of quality and health properties. Blanching also represents a useful tool in preventing enzymatic oxidations, which are the main cause of loss in naturally occurring antioxidants in raw material of plant origin. In fact, fruit and vegetables subjected to blanching retain most of their original antioxidant properties.

Lycopene appears to be a relatively stable compound during food processing but it has been reported that heating tomato pulp to produce paste, ketchup, juice and other products, can cause degradation of lycopene and other carotenoids. Other antioxidants naturally present in tomato, such as vitamin C, vitamin E, polyphenolic flavonoids and non-lycopene carotenoids, may play a role in preventing the degradation of lycopene. Thermal processes might break down cell walls and weaken the bonding forces between lycopene and the tissue matrix. Such disruptions in the cell wall fraction may enhance the release of phytochemicals from the matrix. This may make lycopene more accessible or more stable and thus increase the nutritional quality and antioxidant activity of product. An increase in carotenoids by thermal processing has also been attributed to enzymatic degradation which causes weakening in protein-carotenoid aggregates. Destruction of vitamin C is destroyed mainly due to oxidation reactions and the heat applied in the presence of air. The degree of loss of ascorbic acid is closely correlated to the drying temperature used for the production of the end product. The increase in the amounts of some phenolic substances having antioxidant activity was associated with two concomitant events: firstly, the thermally induced extraction of antioxidant molecules previously complexed or polymerized and secondly, the retention of active compounds caused by the inactivation of the enzymes involved in their catabolism.

Introduction

Fruits, vegetables and some non food crops are rich source of natural antioxidants, food colorants and other bioactive natural products. Nutraceuticals and natural food colorants exhibit anti-oxidant, anti-carcinogenic, anti-inflammatory, anti-bacterial and anti-proliferative properties. They tend to enhance ability of the body to fight, cure and prevent diseases. Natural anti-oxidants are particularly important because they scavenge harmful superoxide, hydroxy, and/or peroxide free radicals, and protect the body against a variety of cancers, heart and other life style related diseases. Vegetable like orange carrot, red carrot, black carrot, beet root, tomato, chilli, and fruits like pomegranate, cherries, berries, watermelon and jamun are rich source of natural antioxidants and food colorants. While tomato and red carrot are rich in lycopenes, and carotenes; black carrot is a major source of stable acylated anthocyanins. The dark red colour of beet root is associated with anthocyanin, betacyanin and betaxanthins. Similarly, the red colour of chilli is attributed to the carotenoid pigment capsanthin. Capsaicinoids in chilli are responsible for the hot flavour and anti-inflammatory properties. Antioxidant and other beneficial health effects of pomegranate, cherries, berries, etc. have been attributed to anthocyanins and related polyphenols. Jamun, an under-utilized tropical fruits is rich in anthocyanins and flavonoides. Like anthocyanins, flavonoides and carotenes, lycopene helps to prevent various types of cancer particularly the prostate cancer.

Microalgae represent a promising alternative to renewable source of feedstock for natural food colorants and nutraceuticals and functional food production. With over 40,000 identified species, microalgae are one of the most diverse groups of organisms on earth. They naturally produce large quantities of many biomaterials. Out of the diverse compounds produced by microalgae, the algal pigments such as phycobillins, carotenoids and xanthophylls have gained commercial importance owing to their unique and multiple applications as food and cosmetic colourants, antioxidants and several beneficial effects of nutraceutical and pharmaceutical value. Microalgal research over the years has geared up significantly from outdoor mass culture in algal ponds, refinement of the cultivation process and life cycle assessment, downstream processing of biomass (i.e., harvesting, dewatering, and drying), to extraction, to systems/process scale-up analysis and development of an economic feasibility model for commercialization of algal biomaterials with nutraceutical properties. Among the various microalgae, Spirulina and Haematococcus are the major source of naturally occurring biopigments which are gaining importance as natural pigments in food, drug and cosmetic industries as an alternative to currently used synthetic colour. Improvement in the cultivation methodologies and use of closed photobioreactors of different configurations, fermentation reactors, open reactors and tubular reactors have opened up new vistas for the development of microalgal biotechnology and bioprospecting. The main constraints in non-exploitation for mass cultivation of microalgae are their slow growth rate, low biomass yield and inadequate culture methodologies and these issues remain to be addressed.

Introduction

Functional food or medicinal food is any healthy food claimed to have a health-promoting or disease-preventing property beyond the basic function of supplying nutrients. The general category of functional foods includes processed food or foods fortified with health-promoting additives, like “vitamin-enriched” products. Fermented foods with live cultures are considered as functional foods with probiotic benefits. Functional foods are an emerging field in food science due to their increasing popularity with health-conscious consumers. The term was first used in Japan in the 1980s where there is a government approval process for functional foods called Foods for Specified Health Use (FOSHU) (Benkouider, 2005).

Distinguishing functional foods from dietary supplements and natural or herbal medicine is especially difficult, and cultural perceptions on this vary. Further complications are the large number of terms often used interchangeably and in a confusing manner such as health foods, nutraceuticals, designer foods, pharmafoods, and so forth. Much of the basic fortification (such as iodization of table salt) is no longer considered “Functional food” but new specialized extracts and ingredients for functional food fortification have emerged, including dietary fibres, plant sterols and probiotics. There is growing research on the potential of biotechnology to develop functional foods for improved health effects of the staple foods in developing countries—for example, high-iron rice, high-vitamin A rice, improved oil content in oil crops, and improved protein.

Introduction

Plants contain a variety of substances of nutraceuticals importance which are useful medicines, food additives, cosmetics and health supplements etc. However, low availability under in vivo condition, decreased plant resources, increases in labour cost and other problems in obtaining these high-value added substances from natural plants have pointed toward the use of in vitro techniques for production of the products. Because in vitro cultures are not affected as the production affected under in vivo conditios.This has led scientists and biotechnologists to consider plant cell, tissue and organ cultures as an alternative way to produce the corresponding secondary metabolites.Therefore, studies on the production of useful metabolite by in vitro techniques have been carried out on an increasing scale since the end of the 1950’s. The large scale cultivation of tobacco and various vegetable cells was examined in the late 1950’s and early 1960’s in the U.S., Canada, and Europe. At present, several firms are manufacturing a plant pigment, shikonin, and ginseng cell biomass on a commercial scale and several other products including anti-oxidant products.

Research efforts on in vitro derived extracts is well documented on each of these major product categories: flavors (onion and garlic, peppermint and spearmint, fruit flavors, chocolate aroma, seaweed flavors, vanilla, celery, coffee, spice, sweeteners, and so on); edible colors for foods and medicines (mainly betalains and anthocyanins): non-food pigments for cosmetics and textiles (shikonin, berberine, and various other products); several examples of fragrances and essential oils; and bioactive natural insecticides and phytoalexins useful in current integrated pest management programs. Of course, intensive activity has centered on production of natural drugs of chemoprotective compounds from plant cell culture. Some of the most prominent pharmaceutical products in this latter category include ajmalicine (a drug for circulatory problems) from Catharanthus roseus and taxol (a phytochemical effective in treatment of ovarian cancer) from Taxus species.

Introduction

From centuries, mankind is totally dependent on plants as a source of carbohydrates, proteins and fats for their food & shelter. In addition, plants are valuable source of a wide range of metabolites, which are used as pharmaceuticals, agrochemicals, flavors, fragrances, colors, biopesticides and food additives. In 1985, 3500 new chemical structures have been identified and 2600 derived from the higher plants. Worldwide, 121 clinically useful prescription drugs are derived from plants. Even today, 75% of the world’s population relies on plants for traditional medicine. In the US, where chemical synthesis dominates the pharmaceutical industry, 25% of the pharmaceuticals are based on plant-derived chemicals.

Biotechnology offers an opportunity to exploit the cell, tissue, organ or entire organism by growing them in-vitro and to genetically manipulate them to get desired compounds. Since the world population is increasing rapidly, there is extreme pressure on the available cultivable land to produce food and fulfill the needs. Therefore, for other uses such as production of pharmaceuticals and chemicals from plants, the available land should be used effectively. Chemical engineers are currently developing improved and appropriate bioreactors for the improvement of production systems by adopting techniques of growth and metabolite production coupled with downstream processing of the products. The improvements in molecular biological research have given a new dimension to in-vitro culture as well as for plant improvement, enhancing the yields of the product and resulting in multiple products or producing novel products from genetically engineered plants. Moreover, the need for safer drugs without side effects has led to the use of natural ingredients with proven safety. These factors have laid emphasis on the use of biotechnological methods to enhance the production of pharmaceuticals and food additives in quality and quantity.

Plant in vitro cultures are able to produce and accumulate many medicinally valuable secondary metabolites. Antioxidants are an important group of medicinal preventive compounds as well as being food additives inhibiting detrimental changes of easily oxidizable nutrients. Many different in vitro approaches have been used for increased biosynthesis and the accumulation of antioxidant compounds in plant cells. Antioxidants are capable of stabilizing, or deactivating, free radicals before they attack cells. Antioxidants are absolutely critical for maintaining optimal cellular and systemic health and well-being. Antioxidants are gaining a lot of importance as a panacea for a large number of life-style diseases like aging, cancer, diabetes, cardiovascular and other degenerative diseases etc. The present lecture summarizes the achievements of in vitro technology for the production of antioxidants compounds. The focus will be on the major chemical classes of antioxidants and on the approaches used to improve the in vitro methods for producing these compounds.

Introduction

The mankind in the past two centuries has witnessed several technology revolutions: industrial, agricultural, medical, info-tech, biotech etc. Nanotechnology is recent one in the series. Nanotechnology is the manipulation or self-assembly of individual atoms, molecules, or molecular clusters into structures to create materials and devices with new or vastly different properties. It uses techniques, processes and materials at the supramolecular level, approximately in a range between 1-100 nanometres (nm), in order to create new properties and to stimulate particular desired functionalities. For the food industry, nanotechnology applications include: release systems for pesticides or fertilizers in agriculture; antibacterial or easy-to-clean surfaces in food processing machines; food additives such as anti-caking in salt, powders and coffee creamers; anti-foaming agents for beer; natural colour additives for beverages; encapsulated nutraceuticals(antioxidants, vitamins) for dietary supplements; and, micelle systems for low-fat foods. It is predicted that in the foreseeable future nanotechnology will transform the entire food industry, changing the way food is produced, processed, packaged, transported, and consumed. The word “nano,” derived from the Greek “nano” signifying “dwarf,” is becoming increasingly too vast in terms of its huge potential in agriculture, health and the environment. In the agricultural sector, nanotech research and development is likely to facilitate next stage of development of genetically modified crops, farm and animal protection and production inputs, food processing, nutraceuticals etc. India is bestowed with a variety of crops, vegetables, fruits, flowers but food sector is handicapped due to inefficiencies in production, processing, storage and packaging. Most of the processing and packaging in industry and agriculture leaves behind a trail of environmental hazards, which if not tackled at this juncture, will add to long term ecological problems.

The importance of air in human life is well known as air is required for running different biological processes in human body. Unfortunately, the same life giving air or oxygen can cause harmful side effects like generation of oxidants or free radicals in our body. Reactive oxygen species, by product of respirative cycle of oxidative phosphorylation, are well known to interfere with cytoplasmic function and bio-molecules and for inflicting damages on cellular bio-molecules, which pre-disposes cells to several anomalies like mutagenic aberration, cell ageing, diseases, cancerous tumour growth and development of various lethal diseases such as cardio-vascular diseases, cancer, atherosclerosis etc. (Gorinstein and others 2001; Apak and others 2004). Free radicals disrupt the proper membrane function, denature proteins and inhibit enzyme function. Antioxidants, naturally present in food, play a vital role in trapping the radicals and; therefore, effective in reducing the risk of heart diseases, immune system decline, different inflammatory reactions etc. (Silici and others 2010).

Free radicals are unstable because they are missing an electron and they try to become stable by attaching to cell membranes in the body, snatching away electrons from them. This, in turn, sets free radicle molecules on an electron snatching spree and starts a chain reaction that ultimately leads to cell death. On the other hand, antioxidants (such as carotenoids, anthocyanins, ascorbic acid, flavonols, flavonoids, polyphenols etc.), present in our foods can prevent or slow down the oxidative damage by donating one of their own electrons, ending the electron-stealing reaction. Epidemiological evidences suggests that intake of antioxidants helps in scavenging free radicals, cause of a broad spectrum damages to biological system (Vertuani and others 2002). This has led consumers to look for a diet, derived from natural sources as well as rich in antioxidants as an strategy to counteract the cellular damages wreak by oxidative stress.

Introduction

Modern life style has taken the fitness out of the men. In ancient times, our life was labour oriented, whereas now we have lots of gadgetry to support our day to day living, thus making our life least labour oriented and we are now more susceptible to diseases diabetes, blood pressure and asthma were, until recently, associated with rich people only, however, these have now become common in urban and even rural areas irrespective of social status and age. The minimum physical exercise, coupled with carbohydrate and fat rich and fiber free diet and lots of tension in day to day life has given way to these diseases.

Since ages, plants have been mainstay medicinal support either in the form of herbal therapy (Like Ayurveda, Unani) or in the form of isolated active substances as being used in allopathic system of medicine. As per a WHO calculation, at least 80% population, in developing world, is dependent on direct use of medicinal plants.

Many of herbal therapies are very simple to use. It is therefore worthwhile to know about some of medicinal plants available commonly around us. The herbal preparations from these plants may not be dramatically effective to palliate disease, as allopathic medicines do, however, with judicious and regular use, we can avoid conversion of some of simple diseases into a major treatment problem.

It is fortunate that side effects of allopathic medicines have compelled us to reconsider the use of herbal remedies and physical exercise in the form of ‘Yoga’, aerobics or whatever you call it. The arid region of Northwest Rajasthan, although vegetated sparsely, is rich in medicinal plants. Some of the species, which are abound in the extreme arid ecosystem, have unique curative and therapeutic properties and some of them are getting a lot of mileage as nutraceuticals as well.

Introduction

Functional food is a natural or processed food that contains biologically active compounds like nutraceauticals and phytochemicals which have health benefit and thus, an important source in the prevention, management and treatment of chronic diseases. For centuries consumption of fruits and vegetables has been attributed to beneficial health effects. Research studies stated that approximately one-third of cancer risk in humans could be attributed to diet. Free radical formation of reactive oxygen species (ROS) and subsequent oxidative stress have been correlated to many human disorders including those of the kidney, eye, lung, liver, nervous system, heart and cardiovascular system. Therefore, foods in our diet that can aid in prevention of these diseases are of major interest to both the scientific community and the general public. Biologically active plant chemicals including traditional nutrients have a beneficial effect on human health. Onion and garlic have received considerable attention for their healthful, functional benefits.

Onion and garlic are the most important vegetable crops grown and consumed almost all over the country. Both the crops belong to the family Alliaceae and have gained the importance of a cash crop in recent years. Maharashtra, Gujarat, Uttar Pradesh, Karnataka, Tamil Nadu, Rajasthan, Andhra Pradesh and Odisha are the major onion growing states whereas Madhya Pradesh, Gujarat, Uttar Pradesh and Odisha are the major garlic growing states. India is a leading producer of onion supporting 20.2% of the world production. Onion is grown in kharif, late kharif and rabi seasons whereas, garlic is grown in rabi season only except in Nilgiri Hills where summer crop is also taken. Onion is normally produced from seeds except multiplier onions where production is taken up vegetatively from bulblets. Garlic is produced vegetatively by planting cloves. Both the crops are rich source of bioactive compounds and have outstanding nutritive value. The nutritive value (per 100g edible portion) of onion and garlic are as follows.

Introduction

The primary role of the diet is to provide enough nutrients to meet metabolic requirements while giving the consumer a feeling of satisfaction and well being. Beyond meeting nutrient needs, diet may modulate various functions in the body and may play detrimental or beneficial roles in some diseases. Now a days concept in nutrition is expanding from the past emphasis on survival, hunger satisfaction and preventing adverse effects to an emphasis on the use of foods to promote a state of well being and better health and to help reduce risk of diseases.

In recent years, the words like functional foods, nutraceuticals, phytochemicals are frequently used/found in media. It is important to understand these words and understand their functions in health care.

Introduction

Cactus pear belongs to Cactaceae family, is widely grown in Mexico and distributed in arid and semi arid tracts of several other parts of the world, such as Australia, Africa, USA and the Mediterranean basin and South East Asia. The future of arid and semi-arid areas of the World largely depends on the development of sustainable agricultural systems based on the use of appropriate crops. Suitable crops are those that successfully cope with water shortage, high temperature, variabilities, poor soils, and easy management to provide food and forage to poor farmers and herders. Opuntias fit most of the above described requirements. In addition, Opuntias play an important ecological role in combating desertification as well as producing fruits and vegetables for human consumption, forage or fodder for livestock, biomass for energy purposes, cochineal for carmine production, and numerous by-products (beverages, vegetarian cheese, drugs and cosmetics). They also provide shelter and food for various wildlife species in arid environments. The fruit known as tuna is a fleshy berry, varying in shape, size, and colour and has a consistent number of hard seeds. The fairly high sugar content and low acidity of the fruit makes it palatable and delicious. Cactus pear was largely treated as underutilized crop by the scientific world until the beginning of 1980, when there was a multiplication of research and symposia, resulting in a large number of publications, including books and book chapters. Recent research finding has, in fact, revealed the high content of some chemical constituents, which can give added value to this crop on a nutritional and bioactive profile. High levels of betalains, taurine, calcium, magnesium, and antioxidants are nutraceutically important. Moreover, some of the constituents show promising in terms of health beneficial quality. Cactus pear products could supplement to nutritional security and human health. The diverse genotypes of Cactus pear have different coloured fruit that show the presence of different antioxidants profiles.

Introduction

Khejri (Prosopis cineraria) grows in dry and arid regions of Arabia and in regions of India mainly Rajasthan, Haryana, Punjab, Gujarat, Western Uttar Pradesh and drier parts of Deccan. The tree is locally known as Jandi or Khejri (India), Jand (Pakistan) and Ghaf (Arabic). It is the State tree of Rajasthan, India. Prosopis cineraria are small to medium size evergreen thorny tree. It is also known as “wonder tree” and “king of desert” as all the parts of tree are useful. The importance of the medicinal value of this tree has been highlighted in our ancient literature. The bark of the tree has abortifacient and laxative properties and is also used as a remedy for rheumatism in the central provinces. The leaves are of high nutritive value and locally called ‘’Loong’’. Leaf paste of P. cineraria is applied on boils and blisters, including mouth ulcers in livestock. The smoke of the leaves is considered good for eye troubles. Leaf extracts of P. cineraria have been reported to show antibacterial, antihyperglycemic, antihyperlipidemic and antioxidative activities . Pods are brown to chocolate in colour on ripening, each containing several seeds embedded in sweet dry yellow pulp. P. cineraria pods are locally called ‘’sangar’’ or ‘’sangri’’. The pod is considered astringent in Punjab. Sangri pods are known to prevent protein and mineral deficiency. Cooked pods of Khejri are used as a functional food in Rajasthan, for the amelioration of numerous illnesses. Pods of khejri have been studied for various phytochemicals like alkaloids, saponins, tannins, flavanoids, flavanols and total phenolics. As this plant is found in water stress (or deficient area) so antioxidant potential of pods has also been investigated. Acetone and methanol extracts of Sangri pods are the most potent bioactive extracts. Nutritional analysis shows it as a good source of proteins and minerals like calcium, sodium and potassium. This study demonstrates that P. cineraria pods may be employed as nutraceutical food with rich nutrition, disease prevention and health promoting effects.

Introduction

Foods and nutrients play a vital role in normal functioning of the body. They are helpful in maintaining the health of the individual and in reducing the risk of various diseases. Nutraceuticals and functional foods are broad terms describing “a food (or part of a food) that provides medical or health benefits, including the prevention and/or treatment of a disease”. Nutraceuticals hold promise in clinical therapy as they have the potential to significantly reduce the risk of side effects associated with chemotherapy along with reducing the global health care cost. Food provides not only essential nutrients needed for life but also other bioactive compounds for health promotion and disease prevention. Consumption of fruit and vegetables, as well as grains, has been strongly associated with reduced risk of cardiovascular disease, cancer, diabetes, Alzheimer disease, cataracts, and age-related functional decline. A change in dietary behavior such as increasing consumption of fruit, vegetables, and grains is a practical strategy for significantly reducing the incidence of chronic diseases.

Functional foods that contain significant amounts of bioactive components may provide desirable health benefits beyond basic nutrition and play important roles in the prevention of chronic diseases. The key question is whether a purified phytochemical has the same health benefit as does the whole food or mixture of foods in which the phytochemical is present. Technological progress in manipulating plant metabolism and metabolites, combined with the explosive growth of the “functional food” industry has led to many attempts to enhance the concentration of these health-promoting compounds in specific plant-based foods . “Functional foods” are foods that, by virtue of physiologically active components, provide a health benefit beyond basic nutrition”. Functional/medicinal food is a category of botanical therapeutic available at the grocery store, which can be decrypted as “food engineered or supplemented to provide health benefits”. Thus, the future of plant-based functional foods seems bright and, as a result, grocery and drug stores might eventually look very similar. Optimization of composition of fruits and vegetables would be a very cost-effective method of disease prevention, since diet-induced health improvements would not carry any added costs for the health sector.

Introduction

Phytosterols, phytostanols and their esters are a group of steroid alcohols and esters that occur naturally in plants as nonsaponifiable fraction of plant oils. Phytosterols (including plant sterols and stanols) cannot be synthesized by humans, and all plant sterols and stanols in the human body therefore originate from the diet. They are known to have several bioactive qualities with possible implications for human health. Their properties for reducing blood cholesterol levels, as well as their other beneficial health effects, have been known for many years. It was recognized in the 1950s that plant sterols lower serum concentrations of cholesterol. Plant sterols might also protect against certain types of cancer such as colon, breast and prostate. Scholarly reviews have all confirmed the health benefits and safety of phytosterols.

People with high blood cholesterol levels are typically advised by health professionals to exercise and consume a diet high in fiber and low in saturated fats and cholesterol. Although these measures can reduce blood cholesterol, sometimes they don’t go far enough. Other cholesterol-lowering interventions may be needed, including cholesterol-lowering medicines or adding phytosterol esters to the diet. The primary phytosterols in the diet are sitosterol, stigmasterol, and campesterol and typical consumption of plant sterols is approximately 160 – 400 mg/day. The enrichment of foods with phytosterols is one of the recent developments in functional foods to enhance the cholesterol-lowering ability of traditional food products.

Introduction

Fiber is that part of plant food which is not digested when eaten. Since it is not absorbed into the body, dietary fiber is not a nutrient. Nevertheless, fiber is a very important component of a healthy diet. Fiber, also called “roughage” helps prevent constipation, hemorrhoids and diverticular disease. Some types of fiber have a cholesterol-lowering effect, which could lead to reduced risk of heart disease.

Dietary fiber has been defined as plant cell wall material resistant to the intestinal secretions of the host. This definition gives the appearance of precision and allows gravimetric and chemical measurements. However, a proportion of other non-fibre dietary constituents (e.g, retrograded starch, protein, and fat) pass along the gut, without absorption or metabolism, to the caecum. Chemical analysis or quantitative measurements of the fibre content of specific diets do not allow prediction of their biological action. The physiological effects of dietary fibre depend predominantly on physical properties unrelated in any simple or direct way to chemical composition. Quantitative measurement of dietary fibre does not allow for diverse effects on nutrient absorption, sterol metabolism, fermentation in the colon, and stool weight. We still do not know whether to restrict the definition of “dietary fibre” to non-starch polysaccharides or to include related materials such as resistant starch, lignin, and the complex assemblies typical of plant tissues, where polysaccharides occur in close association with other molecules. An alternative to defining fibre in terms of chemical composition would be to develop measurements of physical properties relevant to physiological action in the intestine. This approach could be extended to other polymeric constituents, in particular proteins (ingested and secreted) and mucous glycoproteins.

Polysaccharides are the main constituents of dietary fibre and show a wide spectrum of physical, properties that reflect the nature and extent of intermolecular association. Dietary fibre can be likened to a water-laden hydrated mass passing along the gut. Physical properties that influence function are a combination of the rheological and colligative properties of the water-soluble fibre components, the surface characteristics of the water-insoluble components, and the network properties of the swollen hydrated components. Such features include viscosity, water holding, cation exchange, organic acid adsorption, gel filtration, and particle size distribution.

Introduction

The incidence of diabetes mellitus is increasing all over the world and is becoming a problem of significant importance. Diabetes mellitus is a disease of CHO metabolism and WHO has declared India as the diabetic capital of the world. Globally diabetes affects 246 million people, which is about 6% of the total adult population. It is the fourth leading cause of death by disease and every 10 seconds, a person dies from diabetes related causes in the world.

The growing number of diabetic patients and the current and probable future need for health care constraints will mean that high quality diabetic care will become increasingly difficult to be delivered in the future. This requires identification & implementation of cost effective treatment measures that will improve diabetes care in the long term, decrease the associated morbidity and reduce the high direct and indirect costs of its management. This can be possible by incorporation of cost effective and efficient medicinal plants in the management of diabetes. Toxicity and side effects of modern system of medicine have highlighted the importance and relevance of traditional medicinal plants. India has about 45,000 plant species and several thousands have been claimed to possess medicinal properties. Among Indian traditional medicinal plants several potential anti-diabetic plants and herbs are being used as part of our diet since prehistoric time.

In India, medicinal plants have been used as natural medicine since the days of Vedic glory. Historically, in ‘Atharva-Veda’ (about 200 B.C.) description of medicinal plants was made under a separate chapter ‘Ayurveda’. The medicinal values of plants have been tested by trial and error method for a long time by different workers and opened great opportunities for scientific investigations of herbal medicines for cure of diabetes and its complications.

Medicinal plants used to treat hyperglycemic conditions are of considerable interest for ethno-botanical community as they are recognized to contain valuable medicinal properties in different parts of the plant and a number of plants have shown varying degree of hypoglycemic and anti-hyperglycemic activity. The active principles of many plant species are isolated for direct use as drugs, lead compounds or pharmacological agents. Traditional plant medicines or herbal formulations might offer a natural key to unlock diabetic complications if utilized with care.

Introduction

The present trend in the search for new drugs is to look at natural sources, which are safer for humans and ecofriendly. Of late there has been increased awareness about the detrimental effects of synthetic food supplements, food additives and drugs, and the search for natural alternatives in plants has intensified. In this regard, horticultural crops are particularly appealing since they are not only edible, but also have diverse nutraceutical effects. Nutraceuticals (often referred to as phytochemicals or functional foods) are natural, bioactive chemical compounds that have health promoting, disease preventing or medicinal properties (http://foodsci.rutgers.edu),such as antioxidant, anti-inflammatory, hypoglycemic, hypolipidemic, antimicrobial effects, to name just a few. Most of these properties are attributed to the secondary metabolites present in plants, various phytochemicals including flavonoids, terpenoid, lignans, sulfides, polyphenolics, carotenoids, coumarins, saponins, plant sterols, curcumins, phthalides etc. The medicinal value of our horticultural crops is not a new knowledge, it has been exploited by the traditional systems of medicines like Ayurveda, folklore medicines and ‘kitchen remedies’ since time immemorial.

A functional food is similar in appearance to, or may be, a conventional food that is consumed as part of a usual diet, and is demonstrated to have physiological benefits and/or reduce the risk of chronic disease beyond basic nutritional functions, i.e. they contain bioactive compound.

A nutraceutical is a product isolated or purified from foods that is generally sold in medicinal forms not usually associated with foods. A nutraceutical is demonstrated to have a physiological benefit or provide protection against chronic disease.

Introduction

Antioxidants are substances, which retard or prevent deterioration, damage or destruction caused by oxidation. Fortunately, the body has an army of antioxidants for damage limitation. Antioxidant form an integral part of the nutraceutical market. During the last few years’ research has confirmed that many have the common disease and ailments of 21^st century (Coronary Vascular Disease, diabetes, cataracts, high blood pressure, infertilities, respiratory infection, and rheumatoid arthritis) are associated with tissue deficiency and/or low dietary levels of compounds called antioxidants. The oxygen is consumed in the body during metabolism by process called oxidation. During oxidation free radicals are generated. These free radicals at a molecular level burn everything they touch. Antioxidants are power full electron donors and react with free radicals which damage the biomolecules. The formed antioxidant radical is stable and non-reactive. Antioxidants are quite large in number and diverse in nature which oppose the process of oxidation largely by neutralizing free radicals at relatively small concentrations and also have the potential to inhibit the oxidant chain reactions.

Introduction

While Indian agriculture has evolved to provide food to our ever growing population, nutritional security had remained a distant dream. Vegetables, an important component of a healthy diet, are rich source of nutraceuticals such as flavonoids, carotenoids, vitamins, anthocyanins, polyphenolics etc. Such constituents provide health benefits of disease prevention through antioxidant activity and reduced disease risks. Since perishable food commodities and light sensitive nutraceuticals have a specific shelf life, these run the risk of getting spoilt beyond a specific time. Excessive quantities of these must therefore, be processed and converted into value added products with improved shelf life and nutrition value after fortification with nutraceuticals. Nutraceuticals are also referred to by different terminologies like functional foods, fusion foods, dietary supplements, designer foods etc. Nutraceutical products include food supplements, dietary supplements, value-added food products, processed food commodities as well as non-food supplements such as tablets, soft gels, capsules etc. A survey related to nutraceutical rich vegetables has revealed that farmers are growing only the conventional vegetables and there is little awareness about the use of nutraceutical rich vegetables.

Vegetables are rich sources of important phytochemicals, which provide essential nutrients and enhance the body’s ability to prevent and fight disease. There is a multitude of phytochemicals, in unique combinations, in different vegetables, and each functions differently in the body as anti-oxidant, as anti-allergenic, as anti-carcinogenic, as anti-inflammatory, as anti-viral, and/or anti-proliferative. Being of natural origin, some of the phytochemical pigments are increasingly sought after as food colorants. Some of the nutraceutical rich vegetable crops include red and black carrot, beet root, tomato, chilli, and broccoli.

Introduction

Several tropical indigenous fruits have still remained underexploited due to the lack of awareness of their potential, market demand and low and erratic bearing in many cases. Though wild and domesticated diversity is composed of nearly 3000 tropical fruit species, only a few are cultivated on large scale (Vietmeyer, 1990;Pareek et al., 1998). These species (approximately 600 tropical and subtropical) are better known in their areas of diversity and have not yet been utilized to their full potential inspite of their high economic value. There exists a very rich diversity of fruits across the tropical and sub-tropical regions of the world, with more than 500 species of fruits estimated to be found in Southeast Asia alone. The South and Southeast Asia represents above 300 species of native minor fruits (Arora, 1995). These species are being used by local people as minor fruits as well as for medicinal purposes as described in the Indian system of medicine. If properly harnessed, this diversity has great potential as a source of food besides meeting multipurpose needs of the local communities (Pareek et al., 1998).

Introduction

Humans are able to live and work in almost all the environmental extremes of the planet earth and also in space due to their remarkable physiological adaptability and/or by modification of environment itself. Adequate nutrition plays a key role in adaptation and accordingly nutritional needs vary to a great extent. Nutraceutical, a term combining the words “nutrition” and “pharmaceutical”, is a food or food product that provides health and medical benefits, including the prevention and treatment of disease. Such products may range from isolated nutrients, dietary supplements and specific diets to genetically engineered foods, herbal products, and processed foods such as cereals, soups, and beverages. Nutraceutical is a broad umbrella term used to describe any product derived from food sources that provides extra health benefits in addition to the basic nutritional value found in foods. Products typically claim to prevent chronic diseases, improve health, delay the aging process, and increase life expectancy. Phytochemical profile in important crops such as ber, aonla, pomegranate, date palm, karoda, bael, aloe vera, cactus pear of arid region have been indicated that there is wide variation among primary and secondary metabolites. Thus it has become imperative to look into markers linked to high contents of bioactive compounds rather than size and sweetness of the fruits during exploration and evaluation of the plant germplasm.

Introduction

The semiarid and arid ecosystem is not only neglected in India but also the whole world in respect of socio-economic and scientific development. Middlefon and Thomas, 1997) reported the arid and semi arid covers 47% of land surface of the plant and home town to 1/3 of human population. Conventional plant breeding so far only increased the yield of crops grown under arid tropics at about half the rate achieved for crops grown in temperate regions (Turner, 2004, b). The major crop of other ecosystem are considered as under exploited crop of arid ecosystem. Tomato (Lycopersicon esculentum Mill.), is one such crop, which was domesticated in ancient Peru, has become the most popular and widely consumed vegetable in the world today, for its rich flavor and high nutritional value (Vitamins A and C)

Introduction

Vegetables are essentially important functional foods capable of providing additional physiological benefits such as delaying onset of chronic diseases apart from meeting basic nutritional requirements. Vegetables constitute a significant source of carotenoids, phenolics, vitamin C, flavonoids and glucosinolates which boost the immune mechanism and have medicinal properties. The antioxidants in tomato, onions, carrots, garlic, brussel sprouts, peppers and green leafy vegetables and their potential health benefits are critically discussed.

Oxidative damage to biological molecules like DNA, protein and lipids induced by free radicals is considered to be linked with many degenerative diseases such as cardiovascular, cancer, ocular and neurological such as Alzheimer’s and Parkinson’s. Antioxidants from plant sources preserve an adequate function of immune cells against homeostatic disturbances and act as functional foods. Functional foods are foods which besides fulfilling the basic physiological function, have health promoting effect. There is a considerable epidemiological evidence indicating association between diets rich in vegetables and a decreased risk of cardiovascular disease and certain forms of cancer. Diets rich in fruits and vegetables contain high amounts of antioxidant compounds like vitamin C, vitamin E, carotenoids, flavonoids, tannins and other phenolic constituents which boost the immune mechanism.

Introduction

During the last few decades, interest in Ethnobotany has evolved substantially world wide, particularly in India, Africa, Mexico, South America, and South-East Asia. Ethnobiological information on what and how local people make use of plants and animals have been traditionally gathered and passed on from generation to generation. India represents one of the greatest emporia of ethnobotanical wealth. Like other parts of India, in hot arid region also, rural and tribal communities have precious information about utilization of indigenous plants. Besides identification of plants as food, fodder, medicine etc., there also exists similar experience on life support species for exigencies like drought and famines, which is less utilized so far. Several researchers made contribution on etnobotanical aspects of Thar Desert. During 19^th century, King (1869 and 1870) listed wild plants used as food and vegetables during famine by people of Marwar region of Thar Desert. Afterwards, ethnobotanical aspects of edible plants of this region was attempted by several workers

India is emerging as a major stakeholder in the global horticulture scenario accounting for 10.00 % of the world production of fruits. This has been made possible due to concerted efforts of scientists and progressive farming community. In recent years, there is considerable awareness about the nutritional security and food safety. Underutilized fruit trees not only provide nutrition and income security to the people but can also ameliorate the harsh environment of the semi-arid and arid region. Jamun has been named as diabetes fighter for its hypoglycemic (lowering blood sugar) properties.The minor fruits like bael, karonda, khirni, mahua, tamarind, mulberry, pilu, chironji, phalsa and wood apple have tremendous scope for their proper utilization. These fruits are nutritionally rich in bioactive compounds and some of these are of great medicinal value, processing quality and other uses. They have a growing demand in local as well as export market and for processing besides diversification in agriculture in the wake of global changes in weather conditions.

Introduction

While Indian agriculture has evolved to provide food to our ever growing population, nutrition security still remains a distant dream. Fruits an important component of a healthy diet, are rich source of nutraceuticals. Such health components comprising of flavonoides, carotenoides, vitamins, anthocyanins and other polyphenolics provide health benefits of disease prevention through antioxidant activity and reduced disease risks. Of late there has been an explosion of consumer interest in the health enhancing role of physiologically-active specific food components commonly referred to as nutraceuticals and functional foods.

The term ‘nutraceutical’ was first coined by an American doctor Stephen DeFelice in the 1990s. Today, healthcare market is flooded with different new nutraceutical related terminologies like, cosmeceuticals, biopharmaceuticals, herbaceuticals, ayuraceuticals, dermaceuticals, nutri-cosmetics etc. A review of 200 epidemiological studies showed that cancer risk in people consuming diets high in fruits was only one-half that in those consuming few of these foods. Increased acceptance and use of nutraceuticals and functional foods in the western world has resulted in increased export of such commodities from these countries. World-wide market for nutraceuticals and medicinal plant products today has surpassed US $ 15 billion with the top markets being United States (US $ 3 billion), Europe (US$ 2.4 billion), Japan (US$ 2.7 billion) and Asia (US$ 2.7 billion). It is increasing at the rate of 10% per annum. USA holds a significant share (35%) in world nutraceutical market followed by Japan (25%). As per the US estimates, the market for natural food colorants is estimated at US $ 500 million out of which anthocyanin and lutein together account for US $ 200 millions. Other large, emerging international markets in south and southeast Asia and mid-east gulf countries include UAE, Qatar, Oman and Kuwait. At present nutraceuticals and food supplement based functional foods account for trade worth US$ 170 billions and India’s share in this market is less than one per cent.

Introduction

The hot arid regions are spread over about 31.7 million ha area mainly in the States of Rajasthan, Gujarat, Andhra Pradesh, Punjab and Haryana, which inhabit on an average 61 persons per square km making up a population of nearly 20 million people. The Indian arid zone is characterized by high temperature and low and variable precipitation which limit the scope for high horticultural productivity. However, these conditions greatly favour development of high quality production in number of fruits such as date palm, ber, pomegranate, citrus, aonla, bael, grapes, guava and in vegetables such as cucurbitaceous crops, spices and some medicinal plants. The optimized technologies and inputs could increase the existing low productivity. It is now realized that there is a limited scope for quantum jump in fruit and vegetable production in the traditional production areas. The amelioration of the extreme conditions is also considered vital for life support to the inhabitants of this area. The recent awareness regarding the potential of these ecologically fragile lands for production of quality horticultural produce has not only opened up scope for providing economic sustenance for the people of this region, but also for bringing new areas to increase production through horticulture.

Introduction

Underutilized fruits provide nutrition and sustenance of life to the native communities and prevent widespread malnutrition, combat micronutrient deficiencies and other dietary deficiencies, as these are rich in vitamins, minerals, protein, carbohydrates and fats. Vitamins like A, E and minerals like calcium, magnesium and iron are abundantly available in these fruits, which help in building resistance against diseases. Many underutilized fruits contain more vitamin C and pro-vitamin A than the widely available commercial species. Consumption of these fruits by the rural people directly from the trees further maximizes vitamin intake.

A healthy diet depends on a diversity of foods rich in vitamins and minerals. Whether wild, semi-domesticated or fully cultivated, the underutilized and neglected species are important for the well being of local communities. These fruit trees provide the essential supplements that ensure a balanced nutritious diet. Diet diversity is an extremely important element of nutritional well-being, in part because more essential nutrients are consumed and also because it improves the taste of staple foods thus encouraging greater consumption. One of the most common causes for dietary deficiencies appears to be the decreasing diversity of traditional diets. Underutilized fruits are common snack foods that provide vitamins, minerals, proteins, etc. and play important role in diets.

Sugars and sweetners: Sugars are present in all the actively metabolising plant cells in the form of glucose and sucrose. The under-exploited fruits that yield sugar are black maple, sugar maple, sugar palm, wild date palm, honey palm or coquito palm (Jubaea chilensis), talipot palm, nipa palm, toddy palm, palmyra palm (Table 1).

Nature has bestowed our country with diverse agro climatic and topographic conditions ranging from temperate to humid and from sea bed to snow line. The existing climatic conditions with a plenty of sunshine, nutrient rich soil, abundant water resources and reservoir of trained manpower, enable our country to grow various types of vegetables in tropical, subtropical, temperate, alpine and desert vegetation zones in one or the other seasons during the year.

Vegetables, the cheapest and nutrient-rich food source within the economic reach of poor man, play a vital role in human diet since they provide carbohydrates, protein, fat, minerals, vitamins, fibers and phytochemicals (non-nutrient bioactive compounds), which are essential for making the body immune system strong, detoxifying carcinogens, reducing muscular degeneration and protecting the body from infectious ailments. Vegetables besides having medicinal values also play an important role in nutritional security and national economy of the country since they are quick growing and short duration, and their yield per unit area per unit time is 5-10 times higher than the cereal crops.

Computer played a distinctive role in the growth of most advanced, precise and highly sensitive instruments especially in medical and analytical instrumentation technology. Due to the development of different kinds of software the instrumentation analysis has become easy, rapid and result oriented. Auto sampling, development of new methods for detection analysis and the validity of new developed method have been possible with no trouble. Once the standardization of method is over and saved, it can be applied at any time to integrate the unknown samples and their data calculation. Thus, it avoids repetition in calculation and the end result is time saving technique due to the development of computer-organized system in analysis.

The results produced are more reliable and reproducible through various computer based instruments e.g. scanning machines, blood analyzer, gas chromatography (GC), amino acid analyzer (AAA), atomic absorption spectrophotometer (AAS) and high performance liquid chromatography (HPLC) etc.

HPLC is a technology or a system that works on the basis of software. There have been many definitions of computer shielded HPLC, such as high performance liquid chromatography (HPLC), high-pressure liquid chromatography (HPLC), high-speed liquid chromatography (HSLC), high efficiency liquid chromatography (HELC), and simply liquid chromatography (LC).

Chromatography was first employed by Ramsay (1905) to separate mixtures of gases and vapours. The first scientist to recognize chromatography as an efficient method of separation was the Russian botanist Tswett (1906), who demonstrated that, when a plant extract was carried by petroleum ether through a column consisting of a glass tube packed with calcium carbonate powder, a number of dyes were separated, as shown in Figure 1. He named this analysis method “Chromatographie” after “chroma” and “graphos”, which are Greek words meaning “color” and “to draw,” respectively.

Need of Analysis Technique

There are various methods to determine the concentration of a substance present in the matrix of an organic matter, these methods are very expensive, require expertise, need skilled man power, expensive chemicals and sophisticated equipments. But the volumetric analysis requires very less equipment and its level of accuracy is fairly high. It is very beneficial in the preparation of normal and molar solutions which is the back bone of all the research. Scientific venture is useless without the preparation of accurate standard solutions which is to be used in the estimation of various substances by sophisticated techniques like colorimetry, spectrophotometry, atomic absorption spectrophotometer (AAS), inductively coupled plasma emission spectrophotometer (ICP) etc. So the emphasis has been laid on the preparation of quick and accurate preparation of the standards. Volumetric analysis is the simplest technique by which, we can determine the concentration of a substance in solution form by titration of unknown solution against a known concentration solution. One solution is called the standard (known concentration) and other is called unknown solution. In this, reaction takes place either

The noun vegetable means an edible plant or part of a plant, but usually excludes seeds and most sweet fruits. This typically means the leaf, stem, or root of a plant. Vegetables are eaten in a variety of ways, as part of main meals and as snacks. The nutritional content of vegetables varies considerably, though generally they contain little protein or fat (Woodruff,1995 and Whitaker, 2001) and varying proportions of vitamins such as Vitamin A, Vitamin K and Vitamin B6, provitamins, dietary minerals and carbohydrates. They contain a great variety of other phytochemicals, some of which have been claimed to have antioxidant, antibacterial, antifungal, antiviral and anticarcinogenic properties (Gruda, 2005; Steinmetz and Potter,1996). Some vegetables also contain fiber, important for gastrointestinal function. Some contain important nutrients necessary for healthy hair and skin as well. However, vegetables often also contain toxins and antinutrients such as á-solanine, á-chaconine,(Science direct) enzyme inhibitors (of cholinesterase, protease, amylase, etc.), cyanide and cyanide precursors, oxalic acid, and more (Bad Bug Bock > BBB–Clostridium botulinum). Depending on the concentration, such compounds may reduce the edibility, nutritional value, and health benefits of dietary vegetables. Cooking and/or other processing may be necessary to eliminate or reduce them. Diets containing recommended amounts of fruits and vegetables may help to lower the risk of heart diseases and two type of diabetes. These diets may also protect against some cancers and decrease bone loss. The potassium provided by vegetables may help to prevent the formation of kidney stones.

Spices are defined as “A strongly flavored or aromatic substance of vegetable origin, obtained from tropical plants, commonly used as a condiment”. American Spice Trade Association (ASTA) defines spices as “Any dried plant product used primarily for seasoning purposes”. The word “spice” came from the Latin word “species,” meaning specific kind. The name reflects the fact that all plant parts have been cultivated for their aromatic fragrant, pungent or any other desirable properties including the seed (aniseed, caraway, coriander), leaf (cilantro, kari, bay, mint), berry (allspice, juniper, black pepper), bark (cinnamon), kernel (nutmeg), aril (mace), stem (chives), stalk (lemongrass), rhizome (ginger, turmeric, galangal), root (lovage, horse radish), flower (saffron), bulb (garlic, onion), fruit (star anise, cardamom, chile pepper) and flower bud (clove). Spices are distinguished from herbs, which are leafy, green plant parts used for flavoring purpose. Herbs, such as a basil or orengano, may be used fresh and are commonly chopped in to smaller pieces; spices however are dried and usually ground in to a powder (Anonymous, 2007).

In ancient times, spices were as precious as gold, and as significant as medicines, preservatives and perfumes. India is known as the home of spices and produces a wide variety of spices like black pepper, cardamom (small and large), ginger, garlic, turmeric, chilli and a large variety of tree and seed spices. These have nutritionally insignificant quantities as a food additive for the purpose of flavoring. They are good not only for our taste buds but also for our health. They have medicinal properties and also used as preservatives. Spices are well known appetizers, digestives and considered essential in culinary art all over the world. Some of them have antioxidant properties, while others possess strong antimicrobial and antibiotic activities. Spices increase the secretion of saliva rich in ptyalin, which facilitates starch digestion in the stomach, rendering the meals, which are rich in carbohydrates, more digestible. They inhibit thrombus formation and accelerate thromobolysis (Anonymous, 2007a).

India is the world’s second largest producer of fruits, of which mango, banana, citrus, guava, grape, pineapple and apple are the major ones. Apart from these, fruits like papaya, sapota, aonla, phalsa, jackfruit, ber, pomegranate in tropical and sub-tropical parts and peach, pear, almond, walnut, apricot and strawberry in the temperate parts are also grown in a sizeable area. Date palm and fig cultivation is also finding favour in some areas. The major fruit growing states are Maharashtra, Tamil Nadu, Karnataka, Andhra Pradesh, Bihar, Uttar Pradesh and Gujarat.

Production of fruits in India is 44.04 million tones from an area of 3.72 million hectares. One of the bottlenecks in fruit production is the losses incurred by insect pest and diseases which necessitated the use of pesticides to curb the pest problem. Use of pesticides in India is very low 0.350–0.5 kg/hectare as compared to 7.0 kg/hectare in USA, 2.5 kg/hectare in Europe, 12 kg/hectare in Japan and 6.6 kg/hectare in Korea. Despite of low pesticide consumption in India, there is a great problem of pesticide residues vis-a-vis other countries and these residues have entered into food products and underground water because of non-prescribed use of chemical pesticides, wrong advice and supply of pesticides to farmers by vested interests, non-observance of prescribed waiting period, pre-marketing pesticide treatments during storage and transport, use of sub-standard pesticides, effluents from pesticide manufacturing units, continued use of persistent pesticides for public health programme, lack of awareness and lack of aggressive educational programmes for farmers/consumers.

Plant pathogens are known to cause yield reduction of almost 20 per cent in the principal food and cash crops worldwide (Oorke et al., 1994). These losses may be more severe when highly susceptible cultivars are widely grown in a particular region of the globe. The increased world’s population is posing a major global challenge to provide sufficient quatity of food with high quality fruits and vegetables. Nearly, 570 million US $ is now spent annually on research and development by the leading 15 agricultural Companies to combat with the important diseases of major food, fruits and vegetable crops. There are more than 150 active ingredients registered as fungicides worldwide (Mc Dougal, 1996). In the present era of intensive agriculture, fungicides play an important role in our fight against plant diseases and reduce the losses in yield and quality of farm produce. The global fungicide sale has been estimated to be $ 6.0 billion US. The cereal and vine industries of the Western European have become largest fungicide market followed by North America. However, the fungicide use is not intensive in Asia and the new world.

This article presents the historical development of fungicides, mode of their action, development of resistance to fungicides, fungicidal residue and chemical control of various diseases of horticultural and plantation crops.

1. INTRODUCTION

Chemical pesticides are designed to control or eliminate pests such as insects, rodents, weeds, bacteria, and fungus. Although pesticides have played a significant role in increasing food production and controlling various diseases in the agriculture system but indiscriminate use of pesticides has resulted in contamination of soil, water bodies’ etc. raising deep concern about food safety and human health. Excessive use of these harmful chemicals affects kidneys, developing fetus, and liver immuno-suppression and increased incidence of breast cancer. Some of these pesticides are also reported as mutagenic. Other health related problems such as impaired memory and concentration, severe depression, headache, etc have also been reported.

Through field survey (Kadian et al., 2010) it has been found that chlorpyrifos is a widely used pesticide in household application as well as in horticulture crop in National capital region Delhi. This tempted us to understand the nature of chlorpyrifos (CPF) and its metabolites and assess the suitability of available techniques for detoxification of contaminated soil.