eChapters

1. Introduction

Pteridophytes consist of ferns and their allies and are one of the oldest groups of plants on the earth, and they are commonly known as vascular cryptogams. Pteridophytes have a long history of evolution and were the dominant group of plants during the carboniferous period. The ferns, which constitute major element of the pteridophytes, preferentially grow in shade, moist habitats with moderate temperature but also occur throughout from high altitude in arctic-alpine to rain forests of tropic regions of the world. The most favoured habitat of these plants, however, is tropical mesic environment, where 65% of the living species of fernsthrive vigorously and grow luxuriantly (Page, 1979). Ferns are distinct in having rhizomes, stipes, fronds and are homosporous, whereas the fern allies exhibit scale like leaves and are heterosporous (mega and micro). In ferns, the spore matures into gametophyte that bears both male and female gametangia in a definite pattern. In fern allies, both the mega and microspore mature into female and male gametophyte that bears female and male gametangia, respectively. Pteridophytes are unique in having two different phases-the gametophyte and sporophyte. There has been a wide range of variation in spatial distribution, habitat, growth pattern and morphology of pteridophytes. Fern and fern allies, are comprised of six distantly related classes viz. Lycopodiopsida, Selaginellopsida, Isoetopsida, Equisetopsida, Psiloptopsida and Polypodiopsida (Copeland 1947; Pichi Sermoli 1987).

There are about 12000 species of the pteridophytes belonging to 300 genera in the world, whereas India is represented by about 1200 species (Chandra, 2000). Beddome (1892) during the first quarter of the twentieth century provided a comprehensive account on ferns of British India, and subsequently, Clarke (1880) and Hope (1899, 1900, 1902, 1903, 1904) made significant contributions in taxonomic studies of pteridophytes mainly the ferns of north western India. Several workers (Nayar and Kaur 1974; Dixit 1984; Chandra and Kaur 1987, 1994; Manickam et al. 1992; Khullar 1994, 2000; Chandra 2000; Singh 2003; Singh and Panigrahi 2005a, 2005b) have significantly contributed towards the taxonomy of Indian pteridophytes.

The word PULSATILLA is derived from PULSATUS meaning to beat or to strike as it pulsates from the blowing winds.

The weathercock among remedies. The disposition and mental state are the chief guiding symptoms to the selection of Pulsatilla. It is pre-eminently a female remedy, especially for mild, gentle, yielding disposition. Sad crying readily; weeps when talking; changeable, contradictory. The patient seeks the open air; always feels better there, even though he is chilly. Mucous membranes are all affected. Discharges thick, bland, and yellowish green. Often indicated after abuse of Iron tonics, and after badly- managed measeals. Symptoms ever changing. Thirstless, peevish and chilly. When first serious impairment of health is referred to age of puberty. Great sensitiveness.

Pulsatilla cures chronic colds, fatty dyspepsia, and painful and scanty menstruation. One of the leading indications for the use of Pulsatilla is a thick, bland, yellowish or green discharge from mucous membranes in the body. Such discharges are to be found in nasal catarrh, in expectoration from the ears, the eyes, the lungs, ulcers, leucorrhoea and in gonorrhoea. In most such cases Pulsatilla leads to a cure. In the vagina, however, this discharge is excoriating and not bland.

1. Introduction

Thermal processing treatments are the conventional methods that have been broadly employed in the food industry to guarantee the microbiological safety of food materials at a minimal cost. However, these thermal treatments cause undesirable changes in flavor, nutritive value, texture, or color of the finished product that impact food quality (Pan et al., 2017). Growing the modern consumer’s demand towards the minimally processed and “fresh-like” taste foods has challenged the food industry to develop novel non-thermal preservation techniques as an alternative to thermal processing. 

Inula racemosa is an Asian plant in the daisy family native to the temperate and alpine western Himalayas of Xinjiang, Afghanistan, Kashmir, Nepal, Pakistan. Inula racemosa Hook. f., is a critically endangered perennial herb distributed throughout the Himalaya; commercially useful in pharmaceutical products.

Morphological characteristics

The species is a perennial herb that grows up to 1.5 metres tall & has a scented root and rootstock. Large number of stems ascend from the rootstock’s base. Leaves are leathery, rough above and densely hairy below, 25-50 cm long and 10-12 cm wide, and elliptic-lanceolate in shape, 25-50 cm long and 10-12 cm wide.

Floral biology

Flower heads are yellow with bisexual florets and arise in terminal racemes. Flowering takes place from January to July, while fruiting takes place from October to November. Fruits (achenes) are slender and about 0.5 cm long.

26.1. Origin

• Native to Dalmatia (Yugoslavia), Herzegovina and Montenegro.

26.1. Economic importance

• Natural insecticide from dried flower heads.

• Seed or achene contains Pyrethrin I, II, Cinerin I, II, Jasmolin I, II collectively called Pyrethrins.

26.2. Varieties and types

• KKL-1 released from TNAU.

• Hansa and Jhelum released from CIMAP, Lucknow.

• Sel-2 - developed at the Kodaikanal Centre of CIMAP.

• Two types

? Dalmatian pyrethrum: C. cinerariaefolium, grown on the Easte Coast of the Adriatic Sea.

? Japanese pyrethrum also derived from C. cinerariaefolium.

Scientific name: Chrysanthemum cinerariaefolium L.
Family: Asteraceae
Pyrethrum (Chrysanthemum cinerariaefolium L.) is a hearty perennial flower that thrives in interesting and challenging environments. It has been used for centuries as a natural insecticide. The pyrethrum daisy was first documented 2000 years ago, during the Chou Dynasty in China.
Origin and distribution
Pyrethrum originated in China over 2000 years ago and was later introduced to East Africa, where it is currently grown in Kenya, Uganda, Tanzania, and Rwanda. It is also grown in Tasmania, Australia, Ecuador, and Papua New Guinea.
Botany
Pyrethrum is a white flower-headed, tufted perennial herbaceous plant. It possesses deeply lobed leaves and has a numerous and fibrous shallow root system that extends up to 30 cm. The plant features numerous fairly rigid stems that grow up to 50-80 cm in height, accompanied by blue-green, deeply divided leaves. Notably, these leaves are covered on both sides by a dense, woolly material.

For qualitative and quantitative analysis of phytochemicals, chromatography can be used. Gas chromatography is a powerful means of performing qualitative and quantitative measurements of complex mixtures of volatile substances, which are vaporizable without decomposition.Gas Chromatography-Mass Spectrometry (GCMS) system is a widely used instrument for qualitative and quantitative analysis of solid, liquid, and gaseous samples. Samples are converted into gaseous ions and then separated on the basis of their mass-tocharge ratio. 

The different qualitative chemical tests can be performed for establishing profile of given extract for its chemical composition. The following tests may be performed on extracts to detect various phytoconstituents present in them.

1. Introduction:- Overview of Good Laboratory Practices (GLP)
1.1 Definition and Scope of GLP
GLP refers to a set of standards that govern the organizational processes, personnel responsibilities, and conditions under which laboratory studies are conducted. It aims to regulate research environments and ensure the generation of high-quality, traceable, and reproducible results. The scope of GLP encompasses a wide range of scientific fields including pharmaceuticals, agrochemicals, veterinary products, and environmental safety assessments. Good Laboratory Practices (GLP) regulations were developed by the Organization for Economic Co-operation and Development (OECD) and well adopted by regulatory agencies world-wide. The primary objective of GLP is to promote high standards of accuracy and integrity in non-clinical research to protect human health and the environment.
1.2 Principles of GLP
The fundamental principles of GLP include:
• Study Planning and Protocol Development: Every study must have a clear plan and defined objectives, which should be outlined in a comprehensive protocol.
• Personnel Responsibilities: The roles of study directors, scientists, quality assurance personnel, and support staff are clearly defined.
• Test Systems and Facilities: Laboratories must maintain appropriate facilities and control systems for conducting studies. These include properly calibrated equipment, controlled environments, and standardized procedures.
• Data Integrity and Reporting: Data must be meticulously recorded, verified, and archived, ensuring it is traceable and reproducible.
• Study Documentation and Archiving: All records, raw data, and reports must be stored and accessible for a period defined by regulations, ensuring transparency and reproducibility.

Essential oils are products of secondary metabolism and are secreted by specialized cells located in specified parts of most plants. By virtue of certain characteristic properties, they have wide spread use in perfumery, flavouring and medicine. Essential oils are variable mixtures of terpenoids. Since these oils are produced by plants, composition varies with species and variety of plant besides geography, climate and cultivation practices. Since, chemical components are highly reactive, physical and chemical environment to which oil is subjected to during the processes of its extraction, processing and storage have a great influence on final composition. Entire chain of activities from cultivation, extraction, processing, storage to packing has to be perfectly standardized and regulated to assure end product consistency. Being a valuable item of trade, this is important from the point of view of delivering desired activity as well as safety to consumer.

56.1. Aim

To determine quality characteristics of dried and dehydrated fruits and vegetables.

56.2. Theory

The quality of dried/dehydrated products can be measured by estimating moisture contents, preservatives level, acid insoluble ash contents and most importantly enzyme reactions.

56.3. Estimation of Acid insoluble ash

1. To total ash add 25 ml of dilute HCl (10% w/w)

2. Cover with a watch glass and boil gently over a low flame for 5 minutes

3. Filter and wash the filter paper thoroughly with hot water

4. Return the paper to the original dish, ignite, cool and weigh the insoluble ash

Despite its existence and continued use over many centuries, and its popularity and extensive use during the last decade, traditional medicine has not been officially recognized in most countries. Consequently, education, training and research in this area have not been accorded due attention and support. The quantity and quality of the safety and efficacy data on traditional medicine are far from sufficient to meet the criteria needed to support its use worldwide. The reasons for the lack of research data are due to not only to health care policies, but also to a lack of adequate or accepted research methodology for evaluating traditional medicine235.

The statement from General Guidelines for Methodologies on Research and Evaluation of Traditional Medicines (World Health Organization, 2000) highlights the need for consensuous in addressing the problems related to the quality of botanical drugs, which ultimately is a critical determinant of safety and efficacy of medicine. Further, the distribution and widespread sale of adulterated, misbranded, spurious botanical drug products and marked increase in misleading health claims of these products demands proper regulations on botanical medicine to protect public health236. Considering these two issues development of parameters for quality control standardization and regulatory norms for controlling the growing business of botanicals is a big task. Various regulatory authorities, research organizations and botanical drug manufacturers are constantly contributing to developing guiding principles addressing issues related to quality, safety and efficacy237. In this chapter we have highlighted problems associated with quality control of the botanicals and tried to guide a way towards a globally acceptable quality standard.

Quality Control is very important in biofertilizer production, which determines the efficiency of microbial strains. Generally, the quality control based on BIS are voluntary in nature. However, some of the biofertilizers are brought under the ambit of Fertilizer Control Order 1985 (FCO) during 2006 (Yadav and Chandra, 2014). At present, biofertilizers are under the FCO with specific quality standards. Biofertilizer production and its sales can be regulated and is a mandatory requirement for registration of manufacturing unit with the State Fertilizer Controller (who is generally the Commissioner or Director of Agriculture Department) under the statutory provision of FCO. Moreover, some officers of the Agriculture Department have been declared as Fertilizer Inspectors at the district level, who are authorized to inspect production and storage facilities and draw samples for quality analysis. National Centre of Organic Farming (NCOF), Ghaziabad under the Govt. of India and its six Regional Centre’s located at Bhubaneswar, Bangalore, Jabalpur, Nagpur, Hissar and Imphal have been declared as testing laboratories. Under the provisions of the FCO act, State Governments can also develop their own quality control laboratories and notify them under the FCO (1985). Peat, lignite, peat soil, humus, wood charcoal or similar material favouring the growth of microbial strain could also be used. The FCO specifications for different biofertilizers are presented below:

Preservation methods are generally believed to be responsible for a depletion of naturally occurring antioxidants in food. Processed fruit and vegetables are expected to have a lower health protecting capacity than fresh ones. This is because, up to now, only selected and barely stable antioxidants of nutritional interest (e.g. ascorbic acid) have been commonly assessed as indicators of processing damage. On the basis of these supposed negative effects, in the past few years, the main tool for minimizing eventual processing damage was the strategy of ‘reconstitution’, achieved by the addition or the enrichment of the product with natural antioxidants.

It is well known that food processing can have many effects not all of which result in a loss of quality and health properties. Blanching also represents a useful tool in preventing enzymatic oxidations, which are the main cause of loss in naturally occurring antioxidants in raw material of plant origin. In fact, fruit and vegetables subjected to blanching retain most of their original antioxidant properties.

Lycopene appears to be a relatively stable compound during food processing but it has been reported that heating tomato pulp to produce paste, ketchup, juice and other products, can cause degradation of lycopene and other carotenoids. Other antioxidants naturally present in tomato, such as vitamin C, vitamin E, polyphenolic flavonoids and non-lycopene carotenoids, may play a role in preventing the degradation of lycopene. Thermal processes might break down cell walls and weaken the bonding forces between lycopene and the tissue matrix. Such disruptions in the cell wall fraction may enhance the release of phytochemicals from the matrix. This may make lycopene more accessible or more stable and thus increase the nutritional quality and antioxidant activity of product. An increase in carotenoids by thermal processing has also been attributed to enzymatic degradation which causes weakening in protein-carotenoid aggregates. Destruction of vitamin C is destroyed mainly due to oxidation reactions and the heat applied in the presence of air. The degree of loss of ascorbic acid is closely correlated to the drying temperature used for the production of the end product. The increase in the amounts of some phenolic substances having antioxidant activity was associated with two concomitant events: firstly, the thermally induced extraction of antioxidant molecules previously complexed or polymerized and secondly, the retention of active compounds caused by the inactivation of the enzymes involved in their catabolism.

“Quality Control” constitutes the very backbone of the success in any industry, in any field of activity, may it be agriculture or spices, since quality is never an accident, but, the result of a conscientious effort, as rightly visualized by John Ruskin. The quality of spices is evaluated by the following three types of techniques:
1. Physical or sensory evaluation
2. Proximate physico-chemical composition and determination of chemical constituents
3. Nutritional assessment
In the following table (Table 61) presentation of the five proximate physicochemical compositions of 5 minerals and 5 vitamins in as many as 35 spices is presented. Since most of the spices are used basically in small quantities in both vegetarian and non vegetarian food as “Food Additives”, they contribute directly very little to human nutrition, but, indirectly spices can play a very valuable role in healthy human nutrition by not only helping to increase the appeal and appreciation of the food dishes, but, also in various other ways as agents with nutraceutical and pharmaceutical properties. For instance, Turmeric has been found to have immense pharmaceutical and nutraceutical properties. Most recently, it has been found to be very beneficial during the covid pandemic. Excepting in most restrictive diets, spices are regularly used in most food preparations and they can make a difference which persuades a patient to stay faithful to an otherwise unappealing diet. The analysis confirm that even the highest calorie spice, Poppyseeds, will add no more than 2 or 3 calories per serving in normal usage. This should be good news, in particular, to weight – watchers. Considering the amounts used, the others typically contribute no more than a calorie per serving, and, usually less than that. Having this definitive data at last, nutritionists and all those interested in good nutition may utilize spices to their full potential in making wholesome foods as excitingly delicious as they are good for us.

1. INTRODUCTION

Quality, i.e. the degree of excellence or superiority, is a combination of attributes, properties, or characteristics that each commodity value in of human food. The relative importance of each quality component depends upon the commodity and its intended use (e.g. fresh or processed) and varies among producers, handlers, and consumers. Appearance of quality, firmness and shelf-life are important from the point of view of wholesale and retail marketers. Consumers judge quality of fresh fruits and vegetables on the basis of appearance and firmness, flavour (eating) quality of the product. Consumers are also concerned about the nutritional quality and safety of fresh fruit and vegetables.

19.1. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. It is also defined as the combination of attributes or characteristics of the products that has significance in determining the degree of acceptability of the product to a user. Industry defines quality as the measure of purity, strength, flavour, colour, size, maturity, workmanship or any other distinctive attribute or characteristics of the product. According to USDA Marketing Workshop Report (1951) quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user . The quality standards of fresh and processed fruit or vegetable products vary with their intended use. For marketing purposes; size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind. While for processing, physico chemical attributes of raw material such as presence of soluble solids, development of uniform colour, flavour, juiciness, uniform maturity, tenderness in some vegetables etc are taken into consideration. During processing of fruit or vegetables into value added products; colour, flavour and texture etc also become important.

1. Introduction

The interest in the uses of medicinal plants has grown considerably from the mid of 20^th century with the introduction of phytochemical analysis of natural products from various plants parts (Matos, 1996). Most studies survey specific local communities to examine patterns of use of plant part for treating various aliments along with the influence of economic and social variables on plant use (Albuquerque, 2006). Medicinal plants are the base for the development of new drug, which is the basic mode of survival of human and livestock from many diseases to maintain their healthcare (Uddin et al., 2014). Number of studies has been carried out globally on ethnomedicinal uses of plants among various indigenous communities (Alexiades and Sheldon, 1996; Basualdo et al., 1995; Martin, 1995). Particularly from the region Karbi Anglong, Assam (Teron and Borthakur, 2012, 2013; Terangpiet al., 2014; Teronpi et al., 2015) have also conducted many notable work on ethnomedicinal. While in all these studies qualitative approaches have not been adapted to document ethnobotanical information. The quantitative approaches on ethnobotany have grown steadily in the last two decades. Today researchers have developed and applied quantitative methods to the various ethnobotanical data to test different hypotheses about the relation between plant species and humans (Reyes-García et al., 2006). Quantitative ethnobotanical indices were applied to determine how important these plants are to ethnic and indigenous cultures whether, as food, veterinary medicine, human medicine or economic value from various non-timber forest products (Pieroni, 2001; Upadhyay et al., 2011; Reyes-García et al., 2006). Therefore, the aim of this study was to document the various uses of the medicinal plant Geophila repens (L.) I.M. Johnst by the Karbi tribe and to apply quantitative methods to determine the specific importance of these plants for treating particular ailment. G. repens is a very rare plant with high medicinal potential (Roy et al., 2011) and is global distribution is reported from India (Assam, Meghalaya, Andaman Islands and Western Peninsula); Bangladesh; Bhutan; Sri Lanka; Nepal; Malaysia; South China; Fiji; Tropical America; and Africa.

1. The botanical name of West Indian birch is
   1. Bursera simaruba
   2. Bursera serratea
   3. Bursera gumifera
   4. Bursera javanica
2. The following genus is commonly known as khus
   1. Cymbopogan
   2. Chrysopogan
   3. Ocimum
   4. Apium
3. Ceylon citronella indigenous to
   1. India
   2. China
   3. Central Africa
   4. Ceylon

1. Atropa belladonna contains which alkaloids is
   1. 1-hyoscyamine
   2. N-methyl-puridine
   3. Atropine
   4. Pyridine
2. Withania somnifera is commonly known in english
   1. Belladonna
   2. Liquorice
   3. Blue cherry
   4. Winter cherry
3. Botanical name of black nightshade is
   1. Solanum tarvum
   2. Solanum xanthocarpum
   3. Solanum nigrum
   4. Solanum viarum

1. Mostly cultivated arecanut species is
   1. Areca catechu
   2. Areca triandra
   3. Both a and b
   4. None of these
2. Wonder nut is commonly known as
   1. Cashew nut
   2. Cocoa
   3. Coconut
   4. Arecanut
3. The following inflorescence type of cashew is
   1. Racemose
   2. Auxiliary
   3. Cymose
   4. Terminal panicle

1. Which one of the following is the botanical name of dill
   1. Cuminum cyminum
   2. Trychospremum ammi
   3. Apium graveolens
   4. Anethum graveolens
2. The normally in cardamom could be seen in
   1. Throughout year
   2. 4 month
   3. 6 month
   4. 2 month
3. The hortus malabaricus is written by
   1. Ravindran
   2. Subramniyan
   3. Hendrick Van Rheede
   4. Venugopaln Nambiyar

1. Which round of plucking done in tea plantation
   1. 10 – 15
   2. 20 – 25
   3. 45 – 50
   4. 30 – 40
2. The method of oil extraction from fresh coconut was developed by
   1. CFTRI
   2. IARI
   3. IIHR
   4. CPCRI
3. The fresh arecanut fruits are preserved in thick layer of made and this product referred to as
   1. Bura tamul
   2. Chali
   3. Clay preserving
   4. Mud preservation

1. The average dry yield of gymnema is
   1. 2 – 3 kg/plant
   2. 5 – 6 kg/plant
   3. 20 – 25 kg/plant
   4. 10 – 12 kg/plant
2. Isabgol will be ready for harvest in
   1. 80 – 90 days
   2. 60 – 70days
   3. 140 – 150 days
   4. 110 – 130 days
3. The liquorice uprooting in the month of
   1. November – December
   2. February – March
   3. August – September
   4. June – July

1. Ginger rhizomes peeled and soaked in lime water is called as
   1. Garbled
   2. Bleached ginger
   3. Non garbled
   4. Unbleached ginger
2. Harvesting period of cardamom is
   1. October to November
   2. June to July
   3. December to January
   4. March to April
3. Which of following month is flowering period in black pepper
   1. July – August
   2. May – June
   3. November – December
   4. January – February

1. The economic part of bursera is
   1. Root
   2. Leaves
   3. Stem and root
   4. Berries
2. Oil yield of thyme is
   1. 62 kg /ha
   2. 21 kg /ha
   3. 35 kg /ha
   4. 11 kg /ha
3. The oil yield of anise is
   1. 15 kg/ha
   2. 5 kg/ha
   3. 25 kg/ha
   4. 50 kg/ha

1. The leaf roller is an important pest of
   1. Lemongrass
   2. Citronella
   3. Mint
   4. Bursera
2. The following aromatic plants is susceptible to powdery mildew
   1. Sacred basil
   2. Thyme
   3. Geranium
   4. Rosemary
3. Meloidogyne hapla is reported to affect
   1. Thyme
   2. Geranium
   3. Sage
   4. Lavender

1. The major disease of isabgol is
   1. Powdery mildew
   2. Leaf spot
   3. leaf blight
   4. Downey mildew
2. The damping off is a series disease in
   1. Ipecac
   2. Aloe
   3. Indian penny wart
   4. Glory lilly
3. The following medicinal plants is affected by termites causes a lot of damage to young plants
   1. Gymnema
   2. Glory lilly
   3. Henbane
   4. Liquorice

1. The Following disease of cocoa is characterized by formation of water soaked lesions on pod either at tip or stalk
   1. Collectotrichum pod rot
   2. Withches broom
   3. Charcoal pod rot
   4. Blight
2. The following species of coffee is affected by white stem borer grown under inadequate shade
   1. Robusta
   2. Arabica
   3. Tree coffee
   4. both a and B
3. The following disease is major in arecanut
   1. Koleroga
   2. Fruit rot
   3. Mohali
   4. All of these

1. Mostly cultivated arecanut species is
   1. Areca catechu
   2. Areca triandra
   3. Both a and b
   4. None of these
2. Wonder nut is commonly known as
   1. Cashew nut
   2. Cocoa
   3. Coconut
   4. Arecanut
3. The following inflorescence type of cashew is
   1. Racemose
   2. Auxiliary
   3. Cymose
   4. Terminal panicle

1. Seed rate of celery is
   1. 0.5 kg/ha
   2. 2.5 kg/ha
   3. 0.25 kg/ha
   4. 1.5 kg/ha
2. Davana seedlings will be ready for transplanting
   1. 10 – 12 weeks after sowing
   2. 6 – 8 weeks after sowing
   3. 4 – 5 weeks after sowing
   4. 2 – 3 weeks after sowing
3. Bursera planting is done at a spacing of
   1. 2 m x 2 m
   2. 4 m x 4 m
   3. 6 m x 6 m
   4. 8 m x 8 m

1. Liquorice is propagated by
   1. Seeds and stem cuttings
   2. Root cuttings and stem cuttings
   3. Seeds, root cuttings and stem cuttings
   4. Seeds alone
2. Belladonna plants will be ready for planting in main field when they attain a height of
   1. 15 – 20 cm after 8 – 12 weeks
   2. 15 – 20 cm after 2 – 4 weeks
   3. 4 – 8 cm after 8 – 12 weeks
   4. 40 – 45 cm after 8 – 12 weeks
3. The cinchona seeds germinate in ___ days after sowing
   1. 60 – 65
   2. 5 – 10
   3. 20 – 40
   4. 10 – 15

1. The following in meters for High Density Planting (HDP) which enhances the productivity of cashew
   1. 4 x 4
   2. 8 x 8
   3. 3 x 2
   4. 2 x2
2. Which of the following month for seed sowing of cocoa
   1. May – June
   2. December – January
   3. July – August
   4. February – March
3. The following week for seed germination in tea
   1. 2 – 3
   2. 8 – 10
   3. 4 – 6
   4. 1 – 2

1. Ginger is generally planted in the month of
   1. January – February
   2. August – September
   3. March – June
   4. October – December
2. Which one of the following slope is preferred for planting of clove
   1. North – West
   2. North – East
   3. South – East
   4. South – North
3. Ginger seedlings will be ready for transplanting
   1. 10 – 15 days
   2. 10 – 15 days
   3. 30 – 40 days
   4. 60 – 70 days

1. Pick odd one out
   1. Nima
   2. Pragati
   3. Jeva 2
   4. Praman
2. The following type of vetiver is high in oil yield but its oil quality is inferior
   1. East Indian Type
   2. North Indian Type
   3. West Indian Type
   4. South Indian Type
3. Vaishnavi is a variety of
   1. Vetiver
   2. Lemongrass
   3. Palmarosa
   4. Patchouli

1. K-8 is a variety of
   1. Aloe
   2. Coleus
   3. Ashwagandha
   4. Buckwheat
2. The following variety is aloe vera
   1. CIM – Sheel
   2. CIM - Sheetel
   3. CIM – Jeevan
   4. CIM - Mithi
3. Haryana Mulhatti -1 is a variety of
   1. Fox Glove
   2. Long pepper
   3. Liquorice
   4. Safed musli

1. The following hybrid of cocoa is a cross between CCRP 1 x CCRP 7
   1. CCRP 6
   2. CCRP 9
   3. CCRP 10
   4. CCRP 8
2. VHC-3 hybrid of coconut is developed by
   1. Aliyar
   2. Veppankulam
   3. Pattukottai
   4. TNAU, Coimbatore
3. Which one of the following varieties of coffee is multiple crosses
   1. Selection 2
   2. Selection 1
   3. Selection 4
   4. Selection 3

1. Pick the odd one out
   1. Athira
   2. Karthika
   3. Suguna
   4. Aswathy
2. Sreekara is variety of
   1. Turmeric
   2. Black Pepper
   3. Ginger
   4. Cinnamon
3. Find the odd one out
   1. HM107
   2. ML-150
   3. CO4
   4. Pusa early bunching

It is a constitutional disease of infancy and childhood, characterized by impairment of the general health, and arrested growth, abnormal proliferation of the developing bone.

Heredity is a frequent predisposing cause. Poor nutrition, poor housing facilities, want of cleanliness, want of sun light are both predisposing and exciting causes. Age plays a great part. Climate also has its influence, more prevalent in moist than in dry climates. Diathesis influences are second only to heredity.

Hyperemia of all the structures of the bone, periosteum, osseous tissue, cartilage and marrow, is the first step in the development of the rachitic lesion. As a result, we have proliferation of cartilage cells, retarded and irregular development of bone, softening of matrix, undue vascularity of the cancellous tissues, and weakness and deformity of the bones in various parts of the body. The weight of the bones is lessened. The vascularity of the bones is increased. In some cases the blue discoloration is due to the increased vascularity may be seen even through the thick scalp. Outside of the bones they may find blurring of striation of muscles, excessive fat in connective tissue between the muscle fibre and rare cases of fatty degeneration of the muscle fibres themselves. The liver is occasionally enlarged the spleen more rarely so.

The Ramayana written by sage Valmiki is one of the great epics of the Sanskrit language. It is most religiously regarded and popular book in Hinduism. The book is believed to be complied between 300 B.C.E. – 200 AD. However, the original story could be as old 500 B.C.E. (Goldman, 1984). There are many other versions of the epic in various regional languages found in India and South Asia (Raghunathan, 1981; Arya,1998; Dutt, 2004; Mukherjee, 2015; Sharma and Arya, 2022).

As per literature references, the name ‘Ramanaya’ is composed of two words, ‘Rama’ and ‘Aya?a’. Rama, the name of the main character of the Epic, has two contextual meanings. In the Atharvaveda, it means 'dark’, dark-coloured, ‘black' and is related to the word ‘ratri’ (night) which means 'darkness of night'. The other meaning, which can be found in the Mahabharata, is 'pleasing’, pleasant, charming, lovely, beautiful. The word ‘Ayana’ means travel or journey. Thus, Ramaya?a means ‘Rama's Journey’ from his kingdom to various places, forests during his life time (Anonymous, 1960; Tulsidas, 1966; Murthy, 2003; Kishore, 2007; Rajagopalachari, 2013).

Ramayana contains over 24,000 couplet verses, in its full form, which are divided into following seven ‘Ka??as’ (booklets) - Balaka??a, Ayodhyaka??a, Ara?yaka??a, Ki?kindhaka??a, Sundaraka??a, Yuddhaka??a, Uttaraka??a and about 500 sargas (chapters). It is regarded as one of the longest epic poems written in history (Keshavadas, 1988; Debroy, 2017; Gupta, 2021).

morally right man in the world? Narada replied that such a man is Rama, son of King Dasharatha of Ayodhya. Valmiki wanted to know more about Rama and proceeded to take bath when he saw two birds in playful mood and was happy. Suddenly, a hunter hiding in the bush shot one of the two birds. Valmiki became painful and chanted some dialogues in curse which ultimately created a new form of sloka. This was the beginning of the main narrative (Goldman, 1984; Sachithanantham, 2004).

The rambutan is native to Malaysia and commonly cultivated throughout the archipelago and southeast Asia. Though a close relative of the lychee and an equally desirable fruit, this member of the Sapindaceae is not nearly as well-known. Botanically, it is Nephelium lappaceum L. (syns. Euphoria nephelium DC.; Dimocarpus crinita Lour.). In the vernacular, it is generally called rambutan (in French, ramboutan or ramboutanier; in Dutch, ramboetan); occasionally in India, ramboostan.

ABSTRACT

Random Amplified Polymorphic DNA (RAPD) markers were used for characterizing eleven banana (Musa AAB Plantain Subgroup) clones using 40 decamer primers. Eight primers, which produced reproducible banding patterns, yielded 42 scorable bands with an average of 5.25 bands per primer. The amplification products ranged in size from 400 to 1500 bp. A genetic similarity matrix was constructed using the Jaccard’s coeffecient method. The pair wise similarity coefficient values varied between 0.3333 and 0.9355. Based on the coefficients, distances between the clones were computed using SYSTAT software package. In the dendrogram constructed by the nearest neighbour (single- link) method (Krzanowski, 1988), all the eleven clones were found grouped under five clusters. Attu Nendran, Changanasseri Nendran, Changazhikodan, Kaliethan, Koonoor Ethan and Quintal Banana formed the largest cluster. Manjeri Nendran and Myndoli formed the second cluster. Padalamurian, Mysore Ethan and Zanzibar formed three separate clusters. Zanzibar, belonging to the Horn Plantain group was different from the rest of the clones.

ABSTRACT

There is a wide variability in the contents of the commercially important chemical compounds of Centella asiatica depending on the source / ecotypes of the plants. The glycosides found in Indian plants include brahmoside, brahminoside and minor amounts of asiaticosides, which vary in their relative contents depending on the ecotypes. An RAPD marker assisted study of ten accessions, collected from different geographical locations of Kerala, viz., Parumala (Kottayam District), Kottayam, Nagercoil (Tamil Nadu), Mananthala (Trivandrum District), Vlathankara (Trivandrum District), Ponmudi (High range, Trivandrum District), Angamaly (Ernakulam District), Punalur (Kollam district), Pattambi (Palakkad district) and Calicut, was conducted. DNA amplification of the accessions was studied using forty decamer primers, involving 43 cycles. Based on the number of bands four primers were selected. The least similarity coefficient of 0.71 was shown by the Pattambi accession, which formed a separate cluster with all other accessions. Nagercoil and Ponmudi accessions showed the highest similarity coefficient of 0.88 with each other.

ABSTRACT

Hevea brasiliensis, the main source of natural rubber (NR) is commercially propagated through bud grafting. Since Hevea brasiliensis is a cross-pollinated tree crop, seeds obtained are highly heterozygous. Earlier, plantations were grown from unselected seeds. But due to poor yield and large tree - to - tree variations in growth and yield in such plantations bud grafting with desirable scions is now being adopted for commercial planting. Genetically divergent seedlings grown from open pollinated seeds are used as rootstocks to graft the selected scion. This can lead to stock - scion interactions. Large intraclonal variations observed in growth and yield of bud-grafted clones of Hevea are attributed to the genetic heterogeneity of the rootstocks. Monoclonal and polyclonal seed gardens have been established in Kanya Kumari District of Tamil Nadu for producing good quality seeds. Monoclonal seeds are collected from monoclonal seed gardens where only one clone is planted in a vast area without any interference from pollen grains from other clones. Polyclonal seeds are collected from polyclonal seed gardens where different clones are planted in an area and the seeds are fertilized by pollen grains from all the clones. It is a general assumption that seedlings grown from polyclonal seeds may be highly heterogeneous than seedlings grown from monoclonal seeds. The objective of the present study was to find out the reliability of this assumption by studying the genetic variations existing among the polyclonal and monoclonal seedlings. RAPD analysis of leaf samples from polyclonal and monoclonal viz, RRIM 600, PB 86 and GT1 seedlings was carried out using ten Operon arbitrary decamer primers that gave informative amplification. The results showed that there was no significant difference in the genetic variations among the polyclonal and monoclonal seedlings.

ABSTRACT

Vitex trifolia L. (Verbenaceae) is known for its medicinal property. Micropropagation can provide the opportunity to obtain large number of homogeneous plants and there are no reports of micropropagation of this species. So, we attempted for in vitro propagation of this species and described here is an efficient and rapid propagation method. This report describes in vitro shoot induction and plant regeneration from mature nodal explants of Vitex trifolia L. on Murashige and Skoog (MS) medium fortified with benzylaminopurine (BAP), kinetin (KN), thidiazuron (TDZ), adenine (ADE), and 2- isopentenyladenine (2 - iP) (0.25-10.0 mM). Multiple shoots differentiated directly without callus mediation within 3 weeks when explants were cultured on medium supplemented with cytokinins. The maximum number of shoots (9 shoots per explant) was developed on a medium supplemented with 5.0 mM BAP. Shoot cultures was established repeatedly by subculturing the original nodal explant on the same medium. Rooting of shoots was achieved on half strength MS medium supplemented with 0.5 mM naphthaleneacetic acid (NAA). Rooted plantlets were transferred to pots containing autoclaved soil and vermiculite mixture (1:1) that showed 90 % survival when transferred to outdoor. Plants transferred to the field have established themselves in the soil and are growing well. The regenerated plants did not show any immediately detectable phenotypic variation. Natural stands of Vitex trifolia are fast disappearing in India because of indiscriminate collection and over exploitation and the outlined procedure here offers a potential system for mass multiplication and conservation of Vitex trifolia.

ABSTRACT

Cashew (Anacardium occidentale L.) is a woody perennial tree of tropical origin, belonging to the family Anacardiaceae. Cashew is being cultivated in India, Brazil and Africa countries. It is well known for its food, noon food and medicinal uses. In vitro clonal propagation of cashew, which is an effective method for large-scale multiplication of trees was tried. Cashew plantlets were regenerated from shoot explants of 2 - 3 year old grafted plants, which were regularly sprayed with Bavistin 0.1 per cent at weekly intervals. Among the different surface sterilization treatments used, a combination of antioxidants, antibiotics and fungicides was found to be better with around 40 per cent contamination free cultures being obtained. MS medium containing full strength macro elements supplemented with Kinetin (5 mg l ^-1), NAA (1mg l ^-1) and Brassinolide (0.1 mg l ^-1) was found to be best for shoot proliferation and shoot elongation. Micro shoots were rooted in vitro at a frequency of 70-80 per cent, when cultured for 4-8 days in quarter MS liquid medium supplemented with IBA (1mg l ^-1) after the pulse treatment with IBA (100 mg l ^-1) for two minutes. The average number of roots ranged from 3 - 5 with an average length of 1.63 cm. About 50 - 60 per cent of the rooted microshoots survived wearing and produced healthy and vigorously growing plants, which were transferred successfully to the field.

ABSTRACT

Asimple and reproducible protocol for the rapid propagation of Clitoria ternatea L., a flowering garden climber cum medicinally valuable plant, was developed through somatic embryogenesis from the leaf explants. Leaf segments excised from 12 days old in vitro raised seedlings on inducing callus by culturing in MS medium containing 0.5 mgl^-12,4-D and 0.1 mgl^-1 BAP and sub-culturing callus to MS medium containing 1.0 mgl^-1 BAP, produced numerous somatic embryos within 30 - 45 days. Embryos germinated and produced plantlets with intact shoot and root when they were cultured in MS medium devoid of any hormones and supplemented with GA₂ 0.1 to 1.0 mgl^-1. Elongation of plantlets could be achieved on hormone free MS medium in about 3 - 4 weeks. Well- grown plants on transfer to pots, containing sterile sand, in a growth chamber and three weeks later to the greenhouse gave about 40 per cent survival.

The genus Dioscorea comprises of approximately 600 species and sustains livelihood around the globe. Air yam, aerial yam, bitter yam, cheeky yam, potato yam, and parsnip yam are all names for Dioscorea bulbifera L., a traditional medicinal plant native to Asia, Northern Australia, America, and tropical Africa. It’s been used in traditional medicine for a long time and has a lot of potential.

Morphological characteristics

It is a perennial herbaceous climber that can reach a height of 10-20 metres and has multiple fibrous roots. The leaves are alternating, broadly cordate, and bright green in colour, with rounded basal lobes arising from one of them. Aerial tubers (bulbils) are circular, smooth-surfaced tubers that grow freely in leaf axils. Annual stems emerge from a tuberous, woody rootstock. These stems either creep along the ground or climb up the surrounding vegetation. Dioscoreaceae subfamilies are distinguished by the presence of bisexual and unisexual blooms. Seeds are trilocular, dark brown, and somewhat winged (1.2-1.6 by 0.5 cm).

Floral characteristics

Flowers are grouped in spikes that are hanging, axillary, solitary, or fascicled. The seeds are winged at the base, while the capsules are oblong and winged. The fruit has three valves and the perianth is tubular. From September to November, the plant blooms and bears fruit.

37.1. Aim

To prepare ready to serve drink from fruits.

37.2. Theory

Ready to serve (RTS) drink is a type of fruit beverage which contains at least 10% of fruit juice and 10 degree Brix total soluble solids (TSS) with 0.3% acidity. It is not diluted before serving, hence it is known as ready to serve beverage. Mango drink, guava drink, pineapple drink are the commercial products available in the market.

Sweetened fruit juice is categorized as ready to serve beverage in which the minimum juice content shall be 85% with a minimum TSS of 10%, while unsweetened juice is a natural juice having 100% fruit juice with natural total soluble solids. These are preserved by heat processing method. Sweetened apple, pear juices are commercially manufactured.

There are several fungicidal molecules available in the market, some of which are specific for certain diseases/pathogens while others are broad spectrum and can be used against a range of diseases. However, for proper control measures a knowledge of disease specific fungicide are very important. Ready-Reckoner for disease specific fungicide (Table 4) makes available the option for selection of alternative fungicide in the management of specific disease.