eChapters

ABSTRACT

Techniques could be standardized for the induction, initiation, maturation and germination of somatic embryoids from the nucellar tissue isolated from 30-45 day old fruits of mono embryonic mango variety Neelum and poly embryonic variety Vellari Manga. The treatment half strength Murashige and Skoog basal medium containing 2,4-D 5.0 mgl^-1, GA3 5.0 mgl^-1, glutamine 400 mgl^-1, coconut water 200 mll^-1, activated charcoal 500 mgl^-1, sucrose 60 gl^-1and agar gl^-1was found to be the best in induction of embryogenic callus. The treatment 2,4-D 4.0 mgl^-1 + BA 1.0 mgl^-1+ GA3 5.0 mgl^-1in half strength MS basal medium supplemented with glutamine 400 mgl^-1, casein hydrolysate 500 mgl^-1, sucrose 60.0 gl^-1, coconut water 200 mll^-1and agar 6.0 gl^-1was the best in initiating somatic embryos from the induced nucellus of the three varieties. In the variety Neelum BA (8.0 and 16.0 mgl^-1) and kinetin (8.0 to 32.0 mgl^-1) were as effective as 2,4-D in inducing somatic embryogenesis. Polyembryonic variety Vellari Manga showed better response to induction treatments than the mono embryonic varieties. B5 major salts with MS minor salts in combination with 40 gl^-1sucrose, 10 gl^-1PVP, 200 mll^-1coconut water, 5.0 mgl^-1 ABA, 100 mgl^-1 casein hydrolysate and 6.0 gl^-1agar were the best suited for the maturation of the somatic embryos. The positive influence of cobalt chloride, casein hydrolysate and coconut water on the germination of embryoids of both mono and poly embryonic mango varieties could be well established. A basal medium consisting of B5 major salts and MS minor salts supported the highest percentage of germination of somatic embryoids in Neelum and Vellari Manga. However, abnormalities in germination were observed in most of the cases. Excellent germination of somatic embryos was observed in a liquid medium consisting of half strength B5 macro salts and full strength MS micro salts and supplemented with GA3 1.0 mgl^-1+ glutamine 400 mgl^-1 + sucrose 30gl^-1.

The pharynx, or back of the throat, frequently becomes inflamed when a person has a cold or an attack of influenza. This inflammation may also involve the tonsils and adenoids if these have not already been removed.

This is due to many causes:

i) Inflammation of thefauces, soft palate and uvula.

Loose Smut
Sphacelotheca cruenta
Diagnostic Symptoms
• Affected plants can be detected before the ears come out.
• Shorter than the healthy plants with thinner stalks and marked tillering.
• Ears come out much earlier than the healthy.
• Glumes are hypertrophied and the ear head gives a loose appearance than healthy.
• Sorus is covered by a thin membrane which ruptures very early, exposing the spores even as the head emerges from the sheath(Fig-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Eumycota

Soursop, Annona muricata L., also called as Bullock’s heart, is a larger fruit than custard apple. The flesh is spinny and has a white fibrous flesh in which large black seeds are embedded. It is completely tropical in habitat. It resembles small jackfruit with spines on the surface. Soursop is native to the West Indies. It is very popular in Cuba and Mexico. It is found in India and many other parts of Polynesia.

Uses

Soursop is used for the preparation of sherbet and other refreshing drinks. In Indonesia, the tender fruit is used as a vegetable. Seeds have insecticidal properties and leaves contain an essential oil. Excellent jelly can be prepared from soursop fruit. Unripe fruit powder is prescribed for chronic dysentery. Buds and flowers are considered to be an excellent remedy for cough. Soursop is used as an astringent and also as a blood complement. It has the use as an antidysentric and vermifuge. A black dye is produced from the dye unripe fruit. Seed oil is used as an insecticide and for making agriculture implement. Essential oils extracted from soursop (pulp) have industrial applications and the oils are also thought to improve the flavour of processed fruit products.

Soybean, a source of 40 per cent good quality protein, 20 per cent oil and other nutrients has great potential in meeting nutritional requirements and extending health benefits through soy based food products to Indian population. Soy foods are nutritious, economical and good for health. Regular food use of about 10 per cent processed soybean flour with cereal flours or 25-30 g properly processed soybean per day in a diet entitles the consumer to derive maximum nutritional and health benefits at a relatively low-price. Soy food products like fullfatsoyflour, soy-based bakery products and dairy analogs (soy milk, soy paneer (tofu), curd (dahi), soyshrikhand, soy amrakhand), being the products of acceptable type to Indian population, have good scope. Different organizations have given input in R&D work on soybean processing. SPU Centre at ICAR-CIAE, Bhopal has been involved in development of number of soybased food products, technology and equipment for preparation of those. To make available the properly processed soy food products to our population, the centre is actively engaged in capacity building of upcoming entrepreneurs and entreprise development for soybean processing for food uses. Around 500 cottage scale soy food enterprises have been established in different states of the country.

1. Rhizoctonia aerial blight / Web blight
Symptoms
• The soybean Rhizoctonia aerial blight is a damaging foliar disease that quickly defoliates plants in warm, humid climates.
• Rhizoctonia solani causes soybean aerial blight, which manifests as leaf and pod spots, leaf blight, defoliation, lesions on the stem and petiole, a mycelium that resembles a cobweb, and sclerotia that form on diseased leaves.
• The plant’s lower or central sections are where infection starts, and it progressively spreads upward.
• The leaves first have wet patches that eventually become green to reddish brown in color before turning tan, brown, or black.
• Small spots at first eventually combine to cover a significant portion of the leaf.
• Affected leaves droop and stick to the pods and stem underneath them during the aerial blight phase, infecting the pods and seeds in the process.

Among many factors influencing fruit bud formation, the relative proportion of carbohydrate and nitrogen in a tree appears to be the only factor that could be controlled by an orchardist. When under normal course tree does not flower; special practices such as ringing, girdling, stripping, notching, bending, water stress (with holding irrigation), root pruning and root exposure etc. are performed with the aim to control the translocation of food like carbohydrates, starch and sugars. Accumulation of food in the branches leads to fruit bud formation resulting into fruitfulness. Before performing the special horticultural practices, the orchardist should know natural growth habit of the plant, time of new vegetative growth and flowering, when it ceases to grow vegetative and mature its woods and when it takes rest

ABSTRACT

Colchicine, one of the most widely used alkaloids is synthesized in Gloriosa species, a plant of great medicinal importance. The seeds, tubers and leaves of six different species of Gloriosa were phytochemically investigated for alkaloids and were found to contain colchicine and colchicoside. These alkaloids were isolated and quantified by HPLC analysis. This is the first report of alkaloids in Gloriosa plantii, Gloriosa lutea, and Gloriosa vuchuria. The colchicine and colchicoside content of the Gloriosa species was found to be organ dependent as there was statistically significant differences (P<0.05) between different organs but not among different species under study. The total alkaloids are organ as well as species dependent as there were statistically significant differences (P<0.05) between all the species as well as organs of different species of Gloriosa under study and its organs.

The screwpines are tropical shrubby plants grown for the highly scented flowers or the ornamental foliage. The ornamental foliage types are generally cultivated in the green house and protected from direct sun. The variegated types loose the colour in a rich soil and hence should be grown in a rather poor soil containing just sufficient nutrients and moisture. Some species are good for potting and indoor decoration. The common screwpine is used as a hedge also (Randhawa and Mukhopadhyay, 1986)

The screwpine (Pandanus), is highly polymorphic with numerous forms and varieties and selected in various countries for specific uses as maintained by vegetative propagation, e.g. P. leram (Plate 14), the Nicober-bread fruit, is grown in the Andaman Islands for its edible fruit providing a starchy food that is enjoyed locally; another species, P. utilis (Plate 15), on the remote Island of Mauritius, provides fibre from the leaves, used in making bags and a species from Polynesia, P.veitchii (Plate 16) is most commonly grown in green houses and outdoors as an ornamental, where the winter weather is mild and in the species the prop roots and branching stems are particularly well developed attracting attention from many people who might not otherwise notice the stranger from the tropics (Lorus and Milne, 1967). However, these three species give a striking foliage effect and especially useful in seaside gardens as P. utilis with green leaves; P. veitchii with banded white and P. leram with even longer leaves (Thomas, 1965). An unarmed form, forma laevis warb., is cultivated for the fragrant bracts of the inflorescence, while forma samak, a group of prickly Pandanus, is preferred for the tough leaves suitable for matting; another form with longitudinal yellow bands on leaves (forma variegatus) is grown for ornamental purposes (Deshaprabhu, 1966; Ambasta, 1986). In the Pacific Islands, some of the forms (e.g pulposus) are cultivated for their edible fruits (Purseglove, 1975; Ambasta, 1986). Burkill (1966) states that the common wild armed form of the sea-coasts was var. littoralis and that flower growers had selected various races for cultivation, which included the prickly var. samak and thronless var. laevis, grown in India, Malay and Indonesia, are used for making mats.

Description of important spices and condiments

Spices: It is defined as vegetable products (non-leafy parts) or mixture thereof, used for flavouring, seasoning and imparting aroma in food. It may be whole form or in ground form e.g. Pepper, Cardamom, Clove, Cinnamon, etc.

Introduction

Spices hold a special place in world trade and were a catalyst in many historical events, discoveries, wars and inventions. It has even been cited as an important element in the spread of knowledge and culture. Spices are non-leafy parts of plants used as a flavoring or seasoning. Asia is known as ‘Land of Spices’ as it is the place of origin, production, consumption and export of most spices. Out of the world’s 70 plant species grown as spices, 13 are considered major spices produced in Asia. Spices had many uses, from adding flavour to food to medicinal properties, but only grew in a few places. In the last decade, the annual average growth in spice production was 4.3% and trade in spices grew at an average of 5.8% /year. More sophisticated consumers, health-conscious eating habits (but also the rise in the amount of processed foods that use herbs and spices as inputs), rising incomes, increased world trade and globalisation are driving this new era of profitability. Spices are ideal commodities for international trade. They are normally exported in raw or dried form and are usually easy to store and transport. Consequently they do not need expensive infrastructure such as cold chain management or advanced storage facilities and they are not particularly time sensitive. In addition, they are recognised as high value products that can be produced relatively easily on small farms and do not require investments in advanced technology and machinery.

Morphological characteristics

Nardostachys species is a perennial rooted herb with a strong woody main root that grows 10-60 cm tall. The rootstock is strong and lengthy, with petioles of withered leaves clinging to it. The stems are pubescent above and glabrate below. Leaves are both Cauline and radical. Cauline leaves are sessile, occur in two to three pairs, are 2.0 cm 0.6 cm in size, decrease in size from base to top, and are oblong or sub-ovate in shape. Radical leaves are large (15-22 cm 1.5-2.5 cm), six to eight in number, longitudinally nerved, slightly pubescent, and narrow down into the petiole, whereas cauline leaves are sessile, occur in two to three pairs, are longitudinally nerved, The jatamansi rhizome is dark brown, tapered, and densely coated with the remains of old leaf bases, giving it the appearance of a heavy beard, and hence the name jatamansi. The fibrous rhizome may be 6-9 cm long with about 20-30 cm-long yellowish taproot. A single root may bear 30-50 rhizomes.

Floral characteristics

Flowering shoots are 30–40 cm tall and produce 40–50 blooms in three to seven condensed cymes at the summit. Flowers are campanulate and range in colour from pinkish red to bluish white. The hairy corolla tube is 6 mm long. The 4-mm-long hairs on the fruit are capped by the sharp, often dentate, calyx teeth. Flowering takes place between June and August, depending on elevation, while fruits takes place between September and October. Seeds are gathered in October when they turn pale brown in colour.

Spine gourd or teasle gourd (Momordica dioica : Roxb Syn.Momordica roxburghii G. Don, M. wallichi, M J Roem) is a rhizomatous, perennial and distinctly dioecious species of family Cucurbitaceae and  grows widely in many parts of India, mostly confined to  Eastern and South Eastern states. Vernacular names are kartoli,kakrol,meethakarela, padora, bhatt karela, palupagal, tumba, tumpai, golkandra, kankad, kankera, bara-karela, kankoda, kankodan, kantola and phalguni. In India it is mainly grown in Orissa, Bihar, and West Bengal .It is  found in nature in the Punjab, Uttar Pradesh, Rajasthan, Madhya Pradesh, Kerala and Maharashtra especially in areas with the least human interference. This is  a delicacy for its typical taste, attributed to high seed protein content. It also contains high amount of carotene (162 µg/100 g of edible portion) (Gopalan et al., 1982) and its tuberous roots have medicinal value too. It is a cheap source of vitamins and minerals (Bhuiya et al., 1977). This vegetable is gaining popularity  because of its high demand in  market. Certain limitations like lack of awareness among  farmers, non-availability of quality planting materials of standard varieties, unpredictable sex ratio in seedling progenies are arresting the expected spread of this nutritious vegetable (Ali et al., 1991).

18.1. Introduction

Spoiled food is defined as the food that has been damaged or has undergone changes so as to render it undesirable/unacceptable/unfit for human consumption. Spoilage of processed products is mainly caused by the action of micro-organisms and due to the physical or chemical changes. The reasons for microbial spoilage include under- processing, inadequate cooling, contamination from leakage through can seams and pre-process spoilage. In under-processed cans (receiving low heat treatment) large number of micro-organisms is expected to be involved in spoilage.

18.2. Causes of food spoilage

Food spoilage or deterioration may be described as loss of weight, softening, souring, rotting, wilting, moulding or a combination of one or more forms. The major causes of food deterioration are: biotic and abiotic factors.

Squash: It is a type of fruit beverage containing at list 25% fruit juice or pulp and 40-50% TSS commercially. It’s also containing about 1% citric acid and 350ppm sulphurdioxide or 600ppm sodium benzoate. Generally it is diluted before serving. Mango, orange, pineapple, lemon, lime, guava, litchi etc are commercially used for squash making using KMS as preservative. The coloured fruit like jamun, passion fruit, peach, phalasa, plum, mulberry, strawberry and grapefruits are also used for making squash but due to bleaching effect KMS is not used as preservative. In such types of fruits sodium benzoate used as preservative.

1. Introduction
Good Laboratory Practice (GLP) is a systematic quality system essential for
ensuring the reliability, consistency, and integrity of laboratory data, particularly when the findings are used for regulatory decision-making. GLP ensures that laboratory studies are conducted in a scientific manner, maintaining high standards of ethical practice, precision, and reproducibility, with a primary focus on ensuring public and environmental safety. Introduced by the U.S. Food and Drug Administration (FDA) in 1978, GLP gained global recognition through the Organisation for Economic Cooperation and Development (OECD), which established guidelines for its implementation. GLP principles are now widely adopted in regulatory testing for pharmaceuticals, chemicals, food safety, and environmental testing. The OECD’s guidance on GLP has been instrumental in developing standards across the globe, ensuring that test results are scientifically valid and can be accepted internationally. In Asia, particularly in India and other developing countries, GLP compliance has gained increasing importance as pharmaceutical, environmental, and chemical industries expand. Regulatory bodies in these regions have gradually aligned their standards with international norms to ensure their competitiveness in global markets. This chapter discusses the key SOPs necessary for GLP compliance and provides a regional perspective, focusing on India and other Asian nations, where GLP frameworks are evolving to meet international standards.
2. Objective of GLP
The overarching objective of GLP is to ensure that laboratory data is accurate, reliable, and reproducible across different studies. The scientific and regulatory communities rely on GLP to ensure that findings from preclinical studies and safety evaluations can be trusted and utilized for regulatory approval.
The key objectives of GLP include
• Accuracy and Integrity of Data: GLP ensures that every aspect of data collection, analysis, and reporting is conducted with integrity and accuracy, preventing errors or falsification that could affect regulatory decisions.

ABSTRACT

The present investigation was undertaken to standardize the DNA extraction methods, to optimize the PCR conditions and generate RAPD’s to study the genetic variability in chrysanthemum. Total genomic DNA was extracted from freshly emerged leaves of chrysanthemum cultivars (Flirt, Nagpur Red, Snow Ball) using different DNA extraction methods (CTAB method, SDS potassium acetate method, SDS miniprep method and urea phenol method). The highest amount of DNA (349μg/g of leaves) was extracted using CTAB method, while it was lowest (130 μg/g of leaves) in SDS miniprep method. The absorbance ratio was high for DNA extracted by CTAB method (1.82) followed by SDS- potassium acetate (1.65) and SDS miniprep (1.60) methods. For standardization of PCR ampl ification conditions, optimization was carried out to determine the optimal concentrations of template DNA, MgCl₂, Taq DNA polymerase and annealing temperature. In general, either no products or products with inconsistent bands were obtained when the concentration of template DNA was 25 ng or MgCl₂ was 1.0 mM with high annealing temperature of 45°C.

ABSTRACT

Asuccessful micro grafting technique in Cashew (Anacardium occidentale L.) was developed using in vitro germinated seedling as root stock and shoots from stage II cultures as scions. Grafting success was dependent on the method of grafting, size of the scion and age of the root stock. Among the methods tried, side and cleft grafting were found to be suitable. Due to the ease of operation and faster union, side grafting was preferred. High levels of graft union were obtained when 2 - 3 cm long shoots from stage II cultures were grafted on to 8 - 9 days old in vitro raised root stocks in full strength MS (Murashige and Skoog) medium supplemented with Kinetin (2.5 mg l^-1), NAA (0.5 mg l^-1) and Brassinolide (1mg l^-1). The very high growth of grafted scion was obtained, when the micro grafts were transferred into MS medium containing full strength macroelements supplemented with Kinetin (5mg l^-1), NAA (1mg l^-1) and Brassinolide (1mg l^-1). The micro grafts after a process of hardening could be successfully transplanted into soil. Micro grafting techniques standardized could be used for rejuvenation of shoot explants of mature tree.

Description and Distribution
Star Anise is the dried, star-shaped fruit derived from Illicium verum which is an evergreen tree attaining a height of 8-15 meter and a diameter of 25 cm. Leaves are entire, 10 -15 cm long, and 2.5-5.0 cm broad, elliptic to oblanceolate; flowers are solitary, white to red colored, fruits are star-shaped, reddish brown in color, consisting of 6-8 capsules (follicles), arranged in a whorl, radiating from the top of a slender short central stem; each follicle is 12-17 mm long, boat-shaped, hard and wrinkled, containing a seed; seeds are brown, compressed-ovoid, smooth, shiny and brittle. The carpels have a pleasant anise-like odor and an aromatic, refreshing, sweet, anise-like taste; the seeds have the same taste, but, to much lesser extent. It is available, whole, and is not usually ground. The plant is indigenous to tropical and sub tropical East Asia. It is extensively cultivated in a limited area with particular ecological factor, in Kwangsi in south-east China, mainland Tonkin in Indo-China, and is mostly imported from China and Indo-China. No import statistics are, however, available for Star Anise. It is now produced to some extent in Arunachal Pradesh in North- East India.
Area and Production
Star Anise is a recent introduction to India, from China, which is the major producer, supplier and exporter of the crop. No organized commercial-scale cultivation has yet been started in the country, but, it is reported to be gradually picking up. According to the latest report by the Deputy Director of The Spices Board, located in Guwahati, in North-East India, about 12.5 tonnes of Star

Star gooseberry also known as Otaheite gooseberry   (Phyllanthus acidus Skeels) is an important underexploited fruit of family Euphorbiaceae. Phyllanthus distichus Muell. Arg., Phyllanthus acidissimus (Blanco) Muell. Arg., Cicca acida Merr., Cicca disticha L. are the synonyms of star gooseberry. Food value of star gooseberry / 100g of edible portion is given below.

West Bengal

In West Bengal, mangroves are present in Sundarbans, the large deltaic complex of three major rivers, the Ganges, Brahmaputra and Meghna, and 62% of total area is shared by Bangladesh and 38% by India. The Sundarban Biosphere Reserve (SBR), at the confluence of the Bay of Bengal covering an area of 9,630 km², lies between 21°40′N and 22°40′N latitudes and 88°03′E and 89°07′E longitudes, spreading in districts of South and North 24-Parganas in West Bengal, India. The major portion of SBR is designated as Reserve Forest, covering nearly 4260 km² and the area under mangrove vegetation is 2112 km² (FSI 2019). Compared to 2017 assessment there has been a decrease of 2 km² area of mangroves. The mangroves and their associates in the SBR exhibit great generic and species diversity and it is abode to rare and highly endangered flora and fauna, as well as, the only mangrove-tiger (Royal Bengal Tiger - Panthera Tigris tigris) kingdom in the world. Recognizing importance and uniqueness, the United Nations Educational, Scientific and Cultural Organization (UNESCO) declared the Indian part of Sundarbans as a ‘World Heritage Site’ in 1987, and also designated the ‘Sundarban Biosphere Reserve’ under the UNESCO Man and the Biosphere (MAB) program in 2001. Recently, Sundarbans has been declared as a Ramasar Site, considering ecological significance. Totally 33 true mangrove species belonging to 21 genera and 13 families have been identified in Indian Sundarbans. Among the mangrove species listed, Rhizophora apiculata, Bruguiera parviflora, Ceriops decandra, Kandelia candel, Aglaia cucullata, Excoecaria indica, Xylocarpus moluccensis, Xylocarpus granatum, Heritiera fomes, Scyphiphora hydrophyllacea, Brownlowia tersa, Nypa fruticans, Acanthus volubilis, Dolichandrone spathacea and Phoenix paludosa are under serious threat in Sundarbans (Kathiresan 2008; Gopal and Chauhan 2006). Particularly Heritiera fomes (locally called Sundari, from which Sundarbans derives its name), Nypa fruticans and Phoenix paludosa are declining rapidly due to the reduction of freshwater input (Gopal and Chauhan 2006).

Coconut is a tall un-branched palm growing to a height of 15-30 m. it has a stout trunkraising from the swollen base which is known as bole. It is surrounded by a mass of fibrous. The stem is terminating into a radiating crown of leaves. Leaves are known as fronds. Palm is monoecious produces one inflorescence is enclosed in a strong tough double sheath called as spathe. Fruit is known as drupe, large in size, one seeded, round, ovoid I shape and the fruit has smooth thin green skin known as exocarp below which there is a thick fibrous hook known as mesocarp. Tall varieties live up to 80 . 100 years and dwarf varieties are live up to 40 . 60 years. Tall varieties begin flowering 5-7 years after planting and dwarf varieties . 3 years after planting

1. The botanical fruit type of coconut is
   1. Drupe
   2. Hesperidium
   3. Berry
   4. Capsule
2. Palm inflorescence are ____
   1. Monoecious
   2. Male
   3. Dioecious
   4. Female

1. Introduction

Biological systematics (biosystematics) deals with the grouping of biological organisms in discrete taxonomic units based on their character states. These character states are determined through morphological, anatomical, ecological, biochemical and molecular biological analyses of specific parameters. If these character states are converted into numerical parameters, we can apply several statistical and numerical methods to achieve appropriate biosystematic inferences. In fact this particular application of statistical and numerical methods has given rise to what we may describe as Numerical Taxonomy, a concept first developed by Robert R. Sokal and Peter H. A. Sneath nearly a half century ago. In recent years the application of numerical methods to taxonomy has received a great deal of attention with the development of the computer and computer technology to the extent that even classical taxonomists are now resorting to the use of such numerical taxonomy methods with the help of computers. In this article the various methods commonly used, their application and specific limitations or otherwise are discussed in the context of their usefulness for classification and taxonomic disposition of the plants.

Introduction

Horticulture is the science of cultivation, management, postharvest handling, value addition and marketing of fruits, vegetables, ornamental, aromatic and medicinal plants, spices and plantation crops. Looking to its important role in nutritional security, poverty alleviation and employment generation, it has rightly been emerged in form of horticulture industry and as one of the most significant sectors in the world. The production of fruits, vegetables and flowers has acquired much importance in recent times due to their increasing demand. Changing lifestyles of people across the globe, attempts to overcome the stressful lifestyle, inclination towards healthy and balanced eating habits have made people include fruits and vegetables in their daily diet. In the case of flowers, the demand for fresh flowers has steadily increased not only for decoration but also for many other purposes like essential oils, cosmetics, aroma therapy, dry flowers, pot-pourries, natural dyes, medicines, etc. Rising incomes and growing consumer interest in a variety of fresh fruits and vegetables year-round is stimulating international trade in horticulture.

1. Introduction

The global population is very cautious about gaining weight and diabetics. Calorie less and sugar free substances are gaining momentum in food sector. The miracle plant stevia is very fit in this scenario as it is both zero calorie and sugar free and one more additional benefit that it is completely natural. Stevia rebaudiana Bert. belonging to the family asteraceae has been used in many countries for thousands of years as a source of natural sweetener eg., in Paraguay and Brazil Central America, Thailand, Korea and China (Megeji et al., 2005, Sivaram and Mukundan, 2003; Jain et al., 2009). It is also known as sweet herb, sweet leaf, honey leaf, candy leaf, honey yerba (Carakostas et al., 2008) and meeti tulsi. It possesses an extensive root system and brittle stems producing small, elliptic leaves. In natural conditions S. rebaudiana grows in a form of a shrub and reaches even 1 m of height. Leaves are the major economic part. They are sessile and oppositely arranged, lanceolate to oblancoelate in shape, and are serrated above the middle. Trichome structures on the leaf surface are of two different sizes, one large and one small (Shaffert and Chetobar 1994). The white florets are, borne in small corymbs of 2–6 in number. Corymbs are arranged in panicles. Dr. Moises Santiago Bertoni discovered this plant in 1888 at Paraguay. In 1905, the plant was scientifically named as S. rebaudiana after a Paraguayan chemist Dr. Rebaudi. (Gupta et al., 2013). The active components of Stevia i.e., steviol glycosides (SGs) includes rebaudioside A (Reb A), rebaudioside C and stevioside (ST) that are 250-300 times sweeter than sugar (Chalapathi & Thimmegowda 1997). Stevia is a short-day plant and its flowering is induced when days are shorter. Long photoperiods stimulates leaf growth and steviol glycoside content. The plant can survive only mild winters of its native regions. It cannot survive in waterlogged areas. Stevia extracts can be used in a wide variety of food and beverage applications. Individual usages and use levels may vary depending on the country. They act synergistically when used in combination with other sweeteners. The higher market demand favors the cultivation and commercialization of this particular species.

15.1. Origin and distribution

• Also known as Cheeni tulsi or Mou tulsi.

• Originated from Seirra Amambay in North eastern Paraguay and adjacent areas in Bolivia and Brazil.

• Widely distributed in U SA and Brazil.

15.2. Economic importance

• Economic part - leaves

• Candy leaf

• Major constituents-Stevioside (3 to 10%), rebaudiside-A (13%), rebaudiside- B and C

The stone fruit crops namely peach, Prunus persica, plum, Prunus domestica, apricot, Prunus armeniaca, almond, Prunus dulcis and walnut, Juglans regia are grown in wide range of climatic conditions ranging from subtropical to temperate regions. Peach and plum are also cultivated in mid and low hills in addition to temperate regions. Peach is the major stone fruit crop followed by plum, apricot and cherry. Walnut and almond on the other hand are major nut crops. A number of insect pests (~80) infest stone and nut fruits in various stages of the tree growth and development and cause direct and indirect loss out. Only few pests are of economic concern, the details of which are given hereunder

Peach leaf curling aphid, Brachycaudus helichrysi (Kalt.), Aphididae: Hemiptera

It is a polyphagous pest reported infesting 175 plant species of 115 genera of 49 families. It is a regular and key pest of stone fruits viz. peach, nectarine, plum, apricot and almond and

7.1. Storage

• Helpful in checking market glut, providing wide selection of fruits, vegetables and flowers to consumer through most part of year i.e. especially during off season.

• Helps in orderly marketing and increases profit to producers/farmers.

• Done to maintain freshness, quality, reduce spoilage and extend their usefulness.

• Huge post-harvest losses due to lack of proper storage facilities.

• Basic principle of storage - to reduce rate of physiological processes like respiration, transpiration, ripening and other biochemical changes.

• Proper storage aims to control disease infection and preserve best quality for consumers.

• Chemical used to extend the shelf life and quality of produce

Introduction

India is bestowed with huge production of fruits, vegetables, cereals, millets, sugarcane along with meat production, milk production, fish production and poultry. Despite such a huge production in India, owing to perishable nature of these produce around 25-30 per cent of the produce is being lost as post harvest loss. The one important reason for this may be the production of the crops are season specific and during the peak season the production is more while demand for consumption is less. It may be possible to cut down losses to great extent, if these produces may be transported from the produce states to those who are not producing due to agro-climatic reasons. So for making transportation of the perishable a successful venture in decreasing post harvest loss, there must be proper storage facilities both at producing site as well as marketing place. The aim of the storages must be to keep the produce in saleable condition during entire storage periods. Some products are stored for short period whereas fresh fruits, vegetables require cold storage. Foods before keeping into the storage room need some pre-storage treatments for better results.

Abstract

The history of the development of the tea plantation industry in the Darjeeling district dates back to the early fifties of the nineteenth century when the English entrepreneurs took lease of extensive land on the mountain slopes of the Darjeeling Himalaya and started tea plantations. During the formative years of the introduction of the plantation industry the region was sparsely populated so, laborers from various parts of India and her neighboring countries were encouraged to settle in the fringe areas of the Tea gardens. Over the last 160 years many changes have taken place in their lifestyle. The main purpose of this paper is to reconstruct the history of Darjeeling tea and to explore the life of people in the Darjeeling region and also to learn how the local populace adjusted themselves with the given environment with the changing scenario of the area.

Fragaria is a genus of flowering plants in the rose family, Rosaceae, commonly known as strawberries for their edible fruits. It is a low perennial creeping herb which is widely grown in temperate and subtropical regions of the world.

There are more than 20 described species and many hybrids and cultivars. Cultivated strawberries are based on two species, Fragaria chiloensis (L) Duch., native to the west coast of North America and Fragaria virginia Duch., native to eastern coast of North America. Hybrids of these two species are ancestors to all the modern strawberries. The most common strawberries grown commercially are cultivars of the garden strawberry, a hybrid known as Fragaria × ananassa. Strawberries have a taste that varies by cultivar, and ranges from quite sweet to rather tart. Strawberries are an important commercial fruit crop, widely grown in all temperate regions of the world.

The major producers of strawberries are USA, Mexico, Poland, Japan, Italy, Israel, Australia, New Zealand and Turkey. In India it was introduced during 1960’s and is grown to a limited extent in Jammu & Kashmir and Himachal Pradesh and some parts of southern India (Karnataka and Ooty).

Cultivars/Varieites: Tioga, Tufts, Surecrop, Fresno, Toro, Heidi, Hood, Northwest, Puget Beauty, Olympus, Ozark Beauty.

STRAWBERRY

White grubs and cut worms

These insects cut the roots and stems of young plants.
 
Hairy caterpillars

Defoliate plants during June-July

1) Leaf Spot
(Mycosphaerella fragariae teleomorph, Ramularia brunnea Peck anamorph). Disease Symptoms
• Older leaves usually show purple spots (3-6 mm in diameter) on the upper surface, sometimes with a slightly darker halo.
• As the spots mature, they become white to gray and surrounded by a brownish halo.
• A typical lesions, uniformly brown without darker border or lighter centers, may form on young leaves.
• Later on, the whole leaf is covered by numerous lesions and become chlorotic, withered and die.
• Fruits are generally not directly infected, but the loss of foliage can reduce their quality and yield (Fig-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Ascomycota
Class : Dothideomycetes

It is known by different names such as Ramularia leaf spot, rust, bird’s eye spot, grey spots, and white spot. It mostly attacks leaves but can also attack fruit stalks and also fruits.

Plants grow in the open in nature, hence are subjected to biotic and abiotic stress.

Biotic stress is due to living components such as:

Insects and pests

Nematodes, mites, snails, slugs etc.

Vertebrates like rats, birds, jackal, bats etc.

The plant experiences biotic stress when root, stem, bark, shoots, leaves, buds, flowers and fruits are damaged by the influence and activity of biotic factors.

Biotic factors Diseases

Like the pests, diseases also cause stress to plants. Diseases are caused by fungus, bacteria and virus. Sometimes the exposed part of insect eaten plant part becomes the entry pathway for fungus, bacteria and virus.

Stress and depression can ruin your life and affect your health. Seeking support, being realistic and planning ahead can help ward off stress and depression.

For some people, the holidays bring unwelcome guests — stress and depression. And it's no wonder. In an effort to pull off a perfect Hallmark holiday, you might find yourself facing a dizzying array of demands — work, parties, shopping, baking, cleaning, caring for kids on school break or elderly parents, and scores of other chores. So much for peace and joy, right?

Holiday stress and depression are often the result of three main trigger points. Understanding these trigger points can help you plan ahead on how to accommodate them.

Here are the three issues that commonly trigger holiday stress or depression:

Strobilanthes (of Acanthaceae) is a morphologically diverse and species rich genus extensively distributed in South and Southeast Asia (Carine,1997 and Moylan et al 2004). Approximately 74 species are found along the Western Ghats of Southern India - Sri Lanka biodiversity hotspot (Carine, 1997). The mass flowering of Strobilanthes with blue coloured flower makes them promising plants for gardens.  The genus is subjected to several systematic revisions recently (Bennett and Scotland 2003, Carine et al. 2000, 2004 Carine and Scotland 1998, 2000, 2002 and Venu 2007). The most distinguishing character of the genera (Strobilanthes) is its monocarpic nature, i.e., flowers after a long period of vegetative growth ranging 1-15 years and in a few cases flowers after 35 years. Bremekamp (1944) used the term ‘plietesials’ for such monocarpic plants that take several years to reach maturity before flowering.

2.1. Structure of fruits and vegetables

2.1.1. Fruit

• Fruit is a part of a flowering plant that derives from specific tissues of the flower, mainly one or more ovaries (Fig. 1). Plant product with aromatic flavors, which are either naturally sweet or normally sweetened before eating.

2.1.2. Vegetables

• It exhibits wide verity of plant structure like edible seeds, roots, stems, leaves, bulbs (Fig. 2), tubers and non-sweet fruits of herbaceous plants.

• Vegetables are grouped into 3 main categories

? Seeds and pods-contain natural wax coating. Eg. Many legumes

? Bulbs, roots and tubers with no coating, but tuber has suberisation

? Flowers, buds, stems and leaves having low shelf life

1. Introduction

The heritage of medicinal plants used in India has an ancient history. The epic of Ramayana witnessed Sanjivani Booti as life saving drug. Two important compendia on medicinal plants i.e., Charaka Samhita and Sushruta Samhita were published between 1000 BC and 600 BC. In these Ayurvedic literatures, uses of more than 1,200 plant drugs along with their action and specific therapeutic applications are mentioned. Charaka made 50 vargas (groups) of drugs while Sushruta made 38 ganas (groups) of drugs (Singh and Gautam, 1997). India, with its diverse agro-climate, harbors large plant-diversity. Eastern Himalayas comprising Darjeeling, Sikkim and adjacent Nepal as well as Bhutan hills are very rich in florastic diversity, several of which are endangered but economically useful medicinal plants with high demand in national as well as in international market (Manandhar, 1999). A great many of these wild Himalayan medicinal plants have found their way into modern bio-pharmaceuticals and bio-cosmetics in recent times (Rai et. al., 2000). One such species is Nepalese Kutki (Picrorhiza scrophulariiflora, Pennell) belongs to family Scrophulariaceae.

P. scrophulariiflora is better known by the name Nepali Kutki. The rhizome of this plant has been found to contain the chemicals like Kutkin, D-mannitol and vanillic acid (Yang, 1996). It is said to have effects on fever, malnutrition due to digestive disturbance, jaundice, diarrhoea and dysentery (Zhang et al., 1994). In India it is valued for their demonstrated value as an antibiotic. They are also used as an adulterant of, or as substitute for, Gentiana kurrooa. Properties that these two species share include the ability to stimulate appetite and gastric secretions (Jain, 1968). In Nepal rhizome of this species is also used as a cathartic, in case of dyspepsia, as a purgative as well as in the treatment of scorpion bites (Anon., 1993). Some of the important active photochemicals are listed in Table 1.

ABSTRACT

The present experiment was conducted in order to develop a protocol for rapid in vitro propagation of pomegranate Cv. Mridula. Shoot tip and nodal segment explants selected from mature plant were cultured in MS medium supplemented with NAA and BAP in different concentration and combinations. To control phenolic browning, sub- culturing of cultures was under taken at an interval of first and third day after inoculation. The MS medium supplemented with NAA 0.4 mg/l + BAP 1.0 mg/l showed highest percentage of shoot differentiation with 77.77 per cent and 81.25 per cent response from shoot tip and nodal segment explant respectively. In the same media, earlier shoot initiation as well as more number of multiple shoots/culture were obtained. The shoots when elongated up to 2.5 - 3.0 cm length were transferred for rooting in the ½ strength MS media supplemented with auxins. There was no significant difference for root induction among the type of auxin used either NAA, IBA or both in combination. The concentration of auxins 0.5 mg/l was found to be optimum. Rooted plantlets after hardening in green house gave 49 per cent survival.

ABSTRACT

Studies on the impact of bio-fertilizers with levels of NPK on bulb yield, dry matter production, nutrient uptake and cost: benefit ratio in onion (cv. Bellary Red) were conducted in medium black soils during kharif and rabi seasons of 1999 and 2000 both under irrigated and rainfed conditions at the Agricultural Research Station, Hiriyur located under Central Dry Zone of Karnataka. The treatments were; T₁-Control, T₂-Azotobacter, T₃-Azotobacter+75% N+PK, T₄-Azotobacter +100%N+PK, T₅-Azospirillum, T₆-Azospirillum+75% N+PK, T₇-Azospirillum+100%N+PK, T₈-VAM, T₉-VAM 75% P+NK, T₁₀- VAM+100%P+NK, T₁₁ ? recommended NPK(125:50:125 kgha-1) and T₁₂-75% recommended NPK. The experiments were laid out in RBD in three replications.

The bio-fertilizers Azospirillum brasilence (500g ha-1) and Azotobacter vinelandii(500g ha-1) were applied to seedlings at transplanting separately by dipping the roots in the slurry for 15 minutes. The VAM fungi - Glomus mossae was applied to soil @ 1 kilogram per square meter area of nursery bed. The recommended dose of FYM @ 25 t ha-1 was applied to all the treatments as basal dose.

The results of the investigations revealed that the plants provided with Azospirillum + 100% N+PK (T₇) produced highest bulb yield (250.29;339.02;232.98 q/ha), highest total dry matter production (7053.96;8992.78;6287.03 kg/ha), maximum uptake of N, P a nd K ( 1 5 7 . 8 8 : 7 3 . 5 6 ; 7 0 . 4 6 N PK k g / ha ) , ( 2 0 6 . 8 6 : 9 6 . 2 5 : 9 1 . 1 0 N PK k g / ha ) , (131.81:50.25:71.24 NPK kg/ha) and also the maximum cost:benefit ratio of 4.00 (1:400), 5.42 (1:5.42), 3.94 (1:3.94) under irrigated condition during kharif and rabi seasons and under rainfed situation during kharif season respectively.

Thus, among all the treatments, application of Azospirillum + 100% N + PK (Azospirillum + 125:50:125 NPK kg/ha) was found to be most remunerative considering the nutrient uptake, bulb yield and total dry matter production and cost:benefit ratio, which gave the highest returns. This level can be followed for Bellary Red onion irrespective of seasons both under irrigated and rainfed conditions in the Central Dry Zone of Karnataka.

ABSTRACT

Basic understanding of plant cell proliferation and differentiation is essential for applying modern techniques of genetic transformation. The lack of understanding about the physiological and molecular mechanisms underlying in vitro morphogenesis, together with the non-availability of reliable morphogenetic markers, is one of the most serious constraints for application of gene transfer technology to plant improvement. Plant tissue culture has therefore remained an empirical science. In recent years, concerted efforts are being made to understand the molecular control of cell differentiation. In light of this, present work was carried out to study the role of CKI1 (cytokinin independent 1) gene, one of the genes implicated in shoot regeneration, by transforming the tomato cotyledons through Agrobacterium mediated transformation with two different gene constructs one with CKI 1 gene and another without CKI1 gene. In both the constructs, nptII gene was used as a selectable marker. Transformation of tomato cotyledon explants with CKI 1 gene resulted in higher regeneration of putative transformant s (8.0%) as compared to transformation of explants without CKI1 gene (2.1%). The results show that CKI1 gene has a role in increasing the regeneration frequency in tomato.

Introduction

Musa species and cultivars are identified mainly on the basis of morphological characters. Somatic mutations and changes brought about by environment are major obstacles to the accurate identification of clones (Kaemmer et al., 1992). Isoenzymatic markers provide an additional tool for the characterization of germplasm and have been successfully employed in banana to provide better understanding of somaclonal variants and to detect genetic differences at clonal level.

Being located within the center of diversity of cultivated banana, the South Indian state of Kerala accounts for a large share of variability, especially the AA, AB, AAB and ABB genomic groups.Nendran, the French plantain cultivar indigenous to Kerala (Jacob, 1952) is represented by many ecotypes/clones. Other prominent cultivar groups include Mysore (AAB), Rasthali (AAB), Neypoovan (AB), Kunnan (AB), Monthan (ABB) and Karpooravalli (ABB). The variability has been collected and conserved in the field at the Banana Research Station, Kannara. These have been characterized morphologically employing per INIBAP/IPGRI Musa descriptors, facilitating the identification of distinct cultivars, clones and synonyms.This project was initiated to survey the isozyme polymorphism in the cultivar groups and to select suitable system for clonal identification.Results of the study employing three enzymatic systems -esterase, diaphorase and peroxidase are presented

ABSTRACT

In vitro culture and cryopreservation techniques are complementary to ex situ conservation of plant genetic resources that are vegetatively propagated or having recalcitrant seeds. An in vitro germplasm conservation study has been conducted on Banana passionfruit (Passiflora mollissima), a plant used in rural medicine. Micropropagated plants survived up to 12 weeks in Murashige and Skoog medium supplemented with 0.5% sucrose. Cryopreservation experiments were carried out on the explants (apical and/or axillary buds), pre-treated with vitrification solutions, dimethyl sulphoxide (DMSO) and PVS2 (Plant vitrification solution-2) with or without desiccation treatment and frozen in liquid nitrogen (LN). The survival and regeneration of apical buds were higher than axillary buds when pre-treated with 15% DMSO. Treatment with 20 % PVS2 for 1 h followed by 100 % PVS2 for 20 min gave maximum shoot recovery (40%) from cryopreserved apical buds. Desiccation alone was not successful but combination of pre-treatment of PVS2 (20% for 1h and 100% for 20min) with 3 h desiccation gave 30% plant survival from frozen apical buds. Regenerated shoots were rooted on MS medium with 1.0 mgl-1 naphthalene acetic acid (NAA) and 0.2 % activated charcoal.

Introduction
A study protocol tool is a very important comprehensive document that deliberately outlines the whole research rationale design and plan for the successful execution of the epidemiological/ an experimental/ a clinical study. It serves as a blueprint for how the study will be conducted, ensuring consistency, transparency, and adherence to ethical guidelines. The document contains the background information available in literatures on the basis of which the researchers plan to conduct the study with objectives that will answer research related questions with ensuring adherence to ethical standards.
Basic Elements of a Study Protocol
The study protocol includes different components including background Information available in literature for research plan, objectives or purpose of conducting the research, explanation of the study design, selection and exclusion of subjects, treatment of subjects, assessment of outcome safety and efficacy. The purpose of conducting research includes questions like why the study needs to be done and what will be its relevance to scientific society or environment. To achieve these objectives the researchers, design their research work for proper execution of research objectives. The study which is to be designed has structured framework for researchers to answer their research questions and obtain reliable and valid results. The study design not only helps the researcher in learning technical skills but also discovering the creative voice (planning and execution). The study design includes different methods, processes and necessary resources for accomplishment of research problems. The study design also incorporates the methods of data collection and statistical analysis which meets the requirements of desired outcome. The implementation of an appropriate study design guarantees a methodical and exacting research process, resulting in more precise findings and the appropriate type of analysis to address the research-related queries. This makes the conclusions more reliable and legitimate. By ensuring that the data are reliable, repeatable, and validated, a well-designed study contributes to the advancement of knowledge. Additionally, it helps to control biases and confounding variables that could affect the results and helps to clarify the methodology of the study, which

Red Rot
Diagnostic Symptoms
• Practically all above ground parts are affected, but stalks are the main targets of stack.
• Spindle leaves (third or fourth)starts drying.
• Further stalks becomes discolored and hollow.
• Diagnostic symptom is th formation of red streaks, interrupted by white patches , inside the stalk.This can be seen by splitting the stalks longitudinally.
• White patches are characteristics of disease, not found in other stalk rots.
• After splitting opened the diseased stalk, a sour smell due to conversion of glucose to alcohol.
• Red longitudinal lesions are also seen on the mid ribs of the leaves on which black color fruiting body (Acervuli) are formed (Fig.1).

The disease was fi rst reported in Java in 1893 and is prevalent in all sugarcane growing countries. In India the disease occurs in an epiphytotic form in Uttar Pradesh and Bihar during 1939-1940 and 1946-1947 and cause severe damage that threatened the existence of sugar industries.

This is a great Hahnemannian anti- psoric. Its action is centrifugal - from within outward - having an elective affinity for the skin, where it produces heat and burning, with itching; made worse by heat of bed. Inertia and relaxation of fibre; hence feebleness of tone chracterizes its symptoms. Ebullitions of heat, dislike of water, dry and hard hair and skin, red orifices, sinking feeling at stomach about 11.00am, cat-nap sleep; always indicate sulphur homoeopathically. Standing is the worst position for sulphur patients it is always uncomfortable. Dirty, filthy people, prone to skin affections. Aversion to being washed. When carefully selected remedies fail to act, especially in acute diseases, it frequently arouses the reactionary powers of the organism. Complaints that relapse. General offensive character of discharge and exhalations. Very red lips and face, flushing easily. Often use in beginning the treatment of chronic cases and in finishing acute ones.