eChapters

Abstract

Toxic plants are a grave threat to existence and bodily functions of humans and animals resulting in death or sickness through accident, unawareness or bad intention resulting in huge economic loss. They have always been part of our daily life and some of them are so prevalent that we do not even doubt their poisonous nature. The study aimed at systematically documenting the precious traditional knowledge regarding the ethnotoxic plants of district Udhampur. Ethnotoxic data was collected by interviewing 125 infomants between the age group 24-95 years. A total of 89 plant species belonging to 69 genera and 43 families were observed to be toxic to either humans or animals or both. Majority of the taxa were growing in wild. Leaves were observed to be the most toxic plant parts. Frequently encountered toxic plant families include Fabaceae, Solanaceae, Asteraceae, Euphorbiaceae, Apocynaceae and Ranunculaceae. The route /mode of entry of toxic chemicals was mainly observed to be ingestion. A total of 62 ailments/ conditions/diseases were reported to be caused in humans or animals or both by the ingestion, contact or inhalation of toxic plants in the present study. These include nausea, vomiting, headache, diarrhoea, hematochezia, abdominal spasms, anorexia, dysuria, deep yellow or no urine, bloat, etc. The present study may serve as a base line data for further research and will go a long way in safeguarding people from being poisoned by stuff that may have healed them.

Abstract

India harbors a wide variety of medicinal plants used traditionally in various remedies to treat livestock ailments. The therapeutic value of these plants attributes due to the concentration of several bioactive compounds present in them. Indigenous tribal communities are the treasurer of age-old traditional knowledge which inhabits Asian country since centuries. District Poonch exhibits a rich repository of traditional knowledge as major part of the population comprised of nomadic tribes such as Gujjars and Bakerwals which are still practicing the primitive traditional ways to treat diseases. In the present investigation, the traditional herbal plants and associated remedies for treatment of various veterinary disorders are presented. A total of 34 plant species belonging to 30 genera and 21 families have been reported which are being used by indigenous people for ethnoveterinary purposes. The majority of ethnoveterinary plants recorded from the district belong to families such as Ranunculaceae and Araceae (4 species each), Acoraceae, Amaryllidaceae, Apiaceae, Fagaceae, Lamiaceae, Primulaceae, Rutaceae (2 species each) and rest of the families members were represented by one species each. The frequently used plant parts are roots (30.23%), followed by tubers (18.6%), leaves (16.28%), fruits and whole plant (13.95% each), stems (4.65%) and seeds (2.33%). It has been observed that numerous formulations are made from these parts which are used for the treatment of various cattle infections such as abdominal colic, allergic infection, anestrous, chest diseases, constipation, cough, digestive troubles, FMD, fracture, galactagogue, gaseous bloat, induce puberty, internal injury, lung disease, pneumonia, quick discharge, removal of Leech, sexual stimulant, skin infection, snake-bite, stomachache and weakness with snake bite being dominant. The present study infers that the documentation of ethnoveterinary knowledge from the district requires utmost attention and may give leads or serves for further biochemical studies for the discovery of novel plant based drugs which will certainly helps in commercialization and betterment of tribals through benefit resource sharing.

4.1. Fruit ripening

• Genetically programmed stage of development overlapping with senescence.

• Ripe when attains full flavour and aroma

• Optimum ripening condition for fruits• Conditions for controlled ripening of fruits at RH of 85-90%

5.1.1. Climacteric fruits

• Apple, apricot, avocado, banana, ber, cherimoya, fig, guava, kiwifruit, mango, melons, pear, peach, plum, persimmon, papaya, tomato, sapota and passion fruit

5.1.2. Non climacteric fruits

• Carambola, cherries, citrus, grape, litchi, loquat, olive, pineapple, pomegranate and strawberry

Difference between climacteric and non-climacteric fruits

Plant hormone ethylene is involved in a number of physiological activities within the plant system, ranging from seed germination to fruit development. Ethylene acts as a key player in accelerating fruit ripening by regulating the activities of different enzymes, and is responsible for shortening the shelf life and associated post-harvest losses during the storage of fruit crops. However, all fruit crops have different ethylene production capacities and vary in their response to ethylene. Hence, effective management of post-harvest ethylene is imperative for addressing the global challenge of reducing fruit losses after harvesting. In recent periods, numerous technologies and compounds have been developed for the suppression of ethylene and for enhancing in-situ oxidation of ethylene or inhibiting its production at a receptor level. Among these emerging techniques, the use of sachets, fumigating agents, catalysis and composite films embedded with nanoparticles has been found to be trending, but the use of some of compounds has been limited due to health risks or consumer unacceptance. However, most of these technologies are very costly and are not affordable for marginal farmers and developing nations. In order to address these issues, long term breeding approaches for shelf-life improvement, targeting the tfs, ERFs have been involved. This manuscript fills the knowledge gap by encompassing all the recent progress made in enhancing the shelf life of fruits by managing ethylene during storage.

ABSTRACT

Soft rot of ginger caused by the fungus Pythium aphanidermatum is a major disease affecting ginger cultivation. To combat invading pathogen in the event of infection plants are known to produce antimicrobial pathogenesis related proteins (PR proteins) prominent among which are antifungal hydrolases like glucanases and chitinases. Since Pythium lacks chitin in its cell wall, increase in level of glucanase in the plant may enhance the resistance of ginger to soft rot disease. Signal molecule like ethylene plays a prominent role in the defense response of plants. The present study was undertaken to investigate the role of ethylene in enhancing the level of glucanase in the ginger system. Detached leaves of Rio de Janeiro variety of ginger were used in different experiments. Leaves were treated with ethephon at different concentrations and different time intervals. Increase in the glucanase activity by ethephon treatment was quantified by enzyme assay using crude protein extract. Glucanase levels showed an initial increase with increasing concentration of ethephon but decreased at higher concentration (5 mg/ml). A longer exposure of detached leaves to ethephon (48 h) resulted in 3.7 fold increase in glucanase activity compared to control. The level of enhancement of the glucanase transcript also followed a similar trend as revealed by RT PCR analysis. Two acidic isoforms of glucanase were detected using native PAGE and their levels were found to be enhanced by treatment with ethephon. An increase in the levels of glucanase may contribute to the plant’s ability to resist infection by Pythium.

12.1. Origin and distribution

• Native of Australia and Malaysia.

• Introduced by British people in Nilgiri hills of Tamil Nadu for fuel and timber.

• Commercially grown in Seychelles, Java, South Africa, Guatemala, Congo and Brazil.

• Eucalyptus tereticornis - Commercial species

• Major growing states - UP, Tamil Nadu, Kerala and Karnataka.

12.2. Economic importance

• Known as spotted gum tree.

• Source of citronellol, hydroxycitronellal and menthol.

• Contains 0.5-4% essential oil; rich in citronella.

• Essential oil - Therapeutic and ayurvedic benefits.

• Used in soaps, mouth wash liquids, massage oils, air fresheners and bug repellents

Description and Distribution
The genus Anethum comprises 3 species, 2 of which are recognized for commercial use, and, yield Dill oil used in medicine.These 2 species are:
1. Anethum graveolens (European Dill)
2. Anethum sowa (Indian Dill)
European Dill is indigenous to Europe and is cultivated in England, Germany, Romania, Turkey ,USA and former Soviet Union. Until recently, it was not grown in India. However, efforts have been made to acclimatize it in Jammu (Kashmir, India) according to the Regional Research Laboratory (RRL, Jammu) and the Indian Agricultural Research Institute, New Delhi, India, Bhubaneshwar (Odisha State) and its exports also have started since 1988-89. The trends in exports are given in the following table (Table 24)
Export Trends Overview of Top 10 Exporting Countries in 2023
In 2023, India was the top exporting country of Dill, followed by Turkiye, South Africa, China, United States, Spain, Germany, Saudi Arabia, Netherlands, United Arab Emirates. This ranking is based on available export data and may not include countries with missing records.

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

1. INTRODUCTION

Food is constantly being rated for its quality either consciously or subconsciously by all people. Consumers choose food on the basis of its quality and their individual likes or dislikes. The attractiveness of food is clearly a quality which is important both to the manufacturer and to the consumer. When consumers make a selection they basically look for food that is attractive in terms of colour, flavour and texture apart from other psychological and social factors, which they may associate with food. The nutritional quality, shelf-life/keeping quality and cost factor are other criteria which may affect their selection.

The distinctive and attractive colour of food is not only pleasing but is also an indicator of good quality and freshness. The senses of taste and odour help us in detecting and appreciating different flavours and deciding on whether we like the food. The feel and texture of food entering the mouth should be firm, juicy, crisp and are extremely important characteristics that dictate the acceptance of a food. Today’s consumers are discerning, demanding, and more knowledgeable about food. They expect safe products having good value and high sensory quality. Therefore, knowing consumer’s preferences and perceptions of the sensory characteristics of food and beverage products is vital to food manufacturers. Without appropriate evaluation, there is a high risk of market failure.

ABSTRACT

An In vitro study was conducted to evaluate the fungicidal efficiency of four selected fungal antagonists against leaf spot pathogens of ivy gourd by dual culture method. The antagonists evaluated were Trichoderma viride, Trichoderma harzianum, Aspergillus niger and Chaetomium globosum. The major leaf spot pathogens of ivy gourd used for the study were Cercospora cocciniae, Colletotrichum gloeosporioides and Alternaria alternata, causing severe infection on the foliage. Among the four antagonists tested, T. viride and T. harzianum were found to be the most effective recording the highest AI value of 1500 against all the three pathogens followed by A. niger. C. globosum was least inhibitory on C. gloeosporioides and A. alternata, but was effective against C. cocciniae. A complete overgrowth of the antagonists on all these pathogens were observed in the case of T. viride, T. harzianum and A.niger. C. globosum showed aversion to the hyphal growth of C. gloeosporioides and A. alternata, whereas in C. cocciniae, a homogenous growth was observed.

5.1 Introduction

Quality is one of the major positioning tools of the commodity for marketability, profitability and for consumer satisfaction. The relative importance of each quality component depends upon the commodity and its intended use and varies among growers, handlers and consumers. To growers a given commodity must have high yield and good appearance and must withstand long distance transportation to market whereas, appearance of quality and longevity is important from the point of view of wholesale and retail marketers. Terms commonly used in measurement of keeping quality of cut flowers are longevity, shelf-life, vase-life, and lasting quality. In addition to these, several criteria have been used for evaluation of post harvest flower quality like:

Epididymal Total Sperm Count

The epididymal sperm is obtained as described above, incubated at 35°C, which is the optimum temperature of rat epididymal sperm. The epididymal f luid is then diluted to a volume of 5 mL of pre-warmed (32°C) the spermatozoa are counted by hemocytometer using the Improved Neubaur (Deep 1/10mm. LABART, Germany) chamber as described by Pant and Srivastava (2003).

Total viable cell/ml = (Average number of sperm per chamber) x 103 x (Dilution Factor)

Liveability and abnormal sperm

Per cent live spermatozoa is estimated by differential staining technique using Eosin-Nigrosin stain (NE) (Campbell et al., 1953). These slides are also used for estimating the per cent abnormal sperm morphologically on the basis of observable abnormalities of head, neck, mid-piece and trail region of the spermatozoa.

The percent live spermatozoa are determined adopting the differential staining technique using Eosin-Nigrosin stain.

• A drop of epididymal semen is taken on a clean, grease free pre warmed glass slide.

• 4-5 drops of Eosin-Nigrosine stain (1% Eosin and 5% Nigrosine in 3% sodium citrate dehydrate solution) is placed near the semen drop.

• Epididymal semen and stain are mixed gently using a blunt fine glass rod.

• After 30 seconds to 1 minute a thin smear is made on a clean, grease free glass slide.

• The smear is examined under oil immersion objective.

Probably since the beginning of human life on the planet, they have used local fruits and vegetables. For a long time, they have enjoyed the fruit of the wild trees without knowing how to successfully produce them. Such native fruit trees have many advantages. These trees have an extraordinary genetic diversity that can be exploited in breeding programs and genetic advancement of domestic varieties (Khadivi et al., 2020). Many indigenous fruits are still highly valuable, and the harvesting and selling of these forest fruits contribute significantly to the local economy of families in the area. The product collected from forest trees is completely healthy and in terms of quality, they may even be equal to “organic fruits”. In a most recently published book chapter entitled organic pomegranate production in Iran, compiled by Alizadeh and Varasteh (2024), wild pomegranates were considered as noble source of healthy food. Given the multifunctional, ubiquitous nature of wild edible fruits the present chapter was compiled to introduce wild fruit trees of northern Iran with a focus on providing more detailed information about wild pomegranates.
Geographical Aspects of Northern Iran Northern Iran
is a geographical region encompassing a relatively large and fertile area, located along the southern border of the Caspian Sea and the Alborz Mountains. Upon closer examination of the map of Iran (Fig. 1), one can identify three northern provinces: Golestan, Mazandaran, and Gilan. These provinces all share borders with the Caspian Sea and the Alborz Mountains. The Alborz mountain range extends from the western border with the Republic of Azerbaijan to the eastern border with Turkmenistan (Fig.1).

1. Introduction

Foods available in nature and, in some cases, the products made from it thereof contain quite an amount of water. This water not only adds volume to the food products but also provides a sight for attack by the micro organisms. Also, removal of water becomes essential if this water interferes with the end product chracteristics. There are several processes in food industries which are designed to get rid of this excess water, such processes are called concentration processes. The processes which can remove water from food substances are,- evaporation, freeze concentration and membrane based processes. All these process have their limitations in rendering solid content to the final product (Fig. 1). Perhaps, these limitations can be overcome by combinig the processes.

The objectives that are fulfilled by the concentration processes can be enumerated as follows:

1. INTRODUCTION

Exotic means anything of foreign origin and something not of native growth. Exotic vegetables are those species introduced to a country or location. Broccoli, Brussels sprout, Knol- Khol, Red Cabbage, Lettuce, Asparagus, Baby Sweet Corn, Chive, Garden beet, Kale, Swiss chard, Parsley, Parsnip, Chinese cabbage and Leek are important exotic vegetables. These generally require cool temperature.

Due to increasing foreign culture and increasing living standards, the demand of exotic vegetables is continuously increasing in cosmopolitan cities and five star hotels. These are good in taste and have high nutritive value. Most of the exotic vegetables can be used in salad and soups. These crops are very remunerative.

In urban areas only few part time grower devote time to the production of exotic vegetable. Exotic vegetable farming is profitable venture that require little capital and become source of livelihood to the farmer. The increase in their output level and profit will contributing to food security in the country. About 50% world population living in the cities. The rapid urbanization poses new challenges for food security. It is hard for most of the cities in the developing countries to provide sufficient employment for rapid increasing population. Some district of Maharashtra like Pune, Nasik & Satara now growing exotic vegetables like asparagus and broccoli. Cherry tomato, leek, lettuce, red cabbage & baby corn are also important as exotic vegetables.

In HP cultivation of Exotic Vegetable like asparagus, broccoli, lettuce, leek, chinese cabbage, snow pea, and parsley start picking up in Theog, Matyana, Saproon Valley, Sirmour, Katrain, Manali, Narkanda & Sanj Valley. These vegetables are beneficial for health as they are rich in phyto-chemicals and neutraceuticals. India is endowed with a wide range of tropical, sub-tropical and temperate agroclimatic conditions, therefore almost all kinds of vegetable crops grown around the world can be cultivated in one or the other corner. There is a lot of potential for exotic vegetables for domestic and export market. Important one are:

ABSTRACT

Tinospora cordifolia, a versatile medicinal species with antipyretic and immunomodulatory properties is one of the indigenous sources of the therapeutically valuable alkaloid, berberine. However, the plant contains only meagre amounts of this alkaloid. Hence upgradation of the content of berberine in Tinospora, through biotechnological interventions assumes importance. Four week old leaf, petiole and stem derived calli of two ecotypes of Tinospora viz., Vellanikkara ecotype and Madurai ecotype, subcultured on to Murashige and Skoog (MS) medium, supplemented with various growth regulators, were incubated in light and screened for the presence of berberine employing thin layer chromatography. MS medium with NAA @ 2 mg l^-1 supplemented with BA or kinetin, each at 2 mg l^-1, was identified as the basal production medium for in vitro production of berberine, yielding 7.55 μg and 7.36 μg berberine respectively, per gram of calli. Calli produced from stem segments registered maximum amount of berberine compared to leaf and petiole derived callus cultures. Increasing levels of sucrose in basal medium did not have a favourable influence on the expression of berberine in the test calli. Reducing nitrate and phosphate content of the basal medium to half their strength resulted in enhanced berberine production in in vitro cultures of both the experimental ecotypes. Favourable influence of precursor feeding on upgrading the content of berberine in leaf and stem derived calli of both the ecotypes of the experimental species was also confirmed in the study.

ABSTRACT

The Plant Growth Promoting Rhizobacteria (PGPR) is a group of rhizosphere-colonizing bacteria producing substances which increase the growth of plants and/or protect plants against pathogens. The use of PGPR has become rather common practice and is on the rise in many regions of the world. Inoculation with PGPR also aims at reducing the application of potent ially pollut ing chemical fertilisers and pesticides. Biocont rol microorganisms have unquestionable potential for managing plant diseases, insect pests and nematodes besides increasing crop productivity. Interest in biological control has recently been intensified because of imminent bans on effective pesticides, widespread development of pesticide resistance in insect pests and plant pathogens and a general need for more sustainable pest control strategies. Active and long-term colonization of the root surface of the plant and effective expression of disease-suppressive traits are generally considered prerequisites for successful suppression of horticultural crop diseases by rhizobacteria. The important mechanism of biocontrol by PGPR involve competition and production of antibiotics such as 2,4-diacetylphloroglucinol (DAPG), pyoluteorin (PLT), pyrrolnitrin, phenazine-1-carboxylic acid (PCA), 2^-hydroxy phenazines and phenazine-1-carboxamide (PCN). Siderophores, including pyochelin and pyoverdine which chelate iron and other metals also contribute to disease suppression by conferring a competitive advantage to biocontrol agents for the limited supply of essential trace minerals in natural habitats. Induced systemic resistance (ISR) is a state of increased defensive capacity developed by the plant when appropriately stimulated, through activation of latent resistance mechanisms induced by diverse agents including rhizobacteria. Microplants are first grown in vitro under aseptic conditions devoid of microorganisms. They are then transferred to a post vitrum substrate for acclimatization. By creating a beneficial rhizosphere in microplants at an early stage, the plants could be better protected against biotic and abiotic stress that occurs in the greenhouse or in the field. The beneficial rhizosphere can be created by biotization of microplants with PGPR. The search for PGPR to control postharvest decay of fruit crops has been actively pursued and promising strategies have been practiced nowadays. Biological control of postharvest diseases of perishable horticultural crops with PGPR is complex and involves a number of mechanisms to suppress the development of the disease. PGPR, being a potential candidate in disease management through multiple mode of action, it becomes highly imperative to know its different role in the management of plant pathogens.

ABSTRACT

In Kerala, the commercial cultivation of tomato is limited due to the bacterial wilt disease caused by the soil borne pathogen Ralstonia solanacearum. The release of bacterial wilt resistant varieties like Sakthi, Mukthi and Anagha has increased the area under tomato. But, these varieties are susceptible to tomato leaf curl virus diseases caused by Germini group virus and transmitted by white fly (Bemisia tabaci). The disease incidence varies from 10.77% to 90.5% and the yield loss is tremendous. Unless the tomato variety resistant to ToLCV disease is resistant to bacterial wilt also, it cannot be recommended for cultivation in the State.

The somaclonal variations have been well exploited in developing disease resistant varieties in many crops. Therefore an experiment was formulated to study the somaclonal variations and to screen the somaclones for resistance to tomato leaf curl virus disease in L. esculentum var. Sakthi and L. pimpinellifolium.

The somaclones of Sakthi and L. pimpinellifolium along with the parental lines were planted in earthen pots containing potting mixture with bacterial wilt sick soil. These were screened for resistance to ToLCV disease by artificial inoculation of the vector Bemisia tabaci inside the cages. The observations were recorded on ex vitro survival, biometric characters and reaction to ToLCV and other major diseases.

The ex vitro survival was 46.2% in Sakthi and 36.6% in L. pimpinellifolium. The somaclones of Sakthi showed wide variation for plant height and spread compared to that of L. pimpinellifolium. Some of the somaclones exhibited ‘gigantism’ with potato leaved plants. The yield of the somaclones of Sakthi were higher than that of parental line.

Abstract

Andrographis paniculata (Burm.f) Nees (Family: Acanthaceae) is one of the most popular and valuable medicinal plants with high trade value. It is out mentioned in various systems of medicine since ancient times in Indian, Malay, Chinese and Thai. Andrographis paniculata is native to Southeast Asian countries i.e., India, China, Taiwan and Sri Lanka. In India it is distributed from Uttar Pradesh, West Bengal, Assam to Mizoram and also cultivated throughout the country. The plant being used as Ayurvedic medicine in various diseases. The principal chemical constituents are lactones, diterpenoid and flavonoids. Andrographolide, neoandrographolide are the principle secondary metabolites of Andrographis paniculata with more than 80 compounds reported till now. Modern studies revealed that Andrographis paniculata has an extensive range of pharmacological activities such as hepatoprotective, anti-inflammatory, antimalarial, antimicrobial, antidiarrhoeal, anti-human immunodeficiency virus (HIV), anti-oxidant and anti-pyretic. Therefore, such plants need value addition for product development.

ABSTRACT

Agrotechnology of banana has been introduced for the first time by CSIR- Indian Institute of Integrative Medicine Jammu for commercial cultivation of banana in Himalayan Shivalik range. The aim of the present investigation is to study the potential of Tissue Culture (TC) banana production in a diverse environment of Shivalik range, to make the state self-sufficient in banana production, employment generation and generate revenue for the farmers and to studies on the post harvest handling and marketing of TC banana. Initially, the sapling of this high quality tissue culture variety {BHIM Grand naine (G-9)} Banana was brought from Agro-division of Cadilla Pharmaceuticals Limited Ahmedabad, Gujarat. The field experimental trial was conducted during 20016- 2017 at CSIR-IIIM Research Station, Chatha Farm, Jammu in which four treatment combination arranged in Randomized Complete Block Design (RCBD). Significant differences were observed among the treatments for all the parameters studied. Among the date of planting, banana planted at 10th August reported significantly highest pseudostem height, pseudostem girth, more average number of leaves/plant, first flower emergence (days), first finger ripening (days), more number of finger per hand, more number of hand/ branch, higher weight of finger/hand (kg), higher weight of single finger (g), more finger diameter (cm), higher length of finger (cm), more weight of finger/ bunch (kg), more percentage of plant harvested at a time, higher yield (64.22 tonnes/ha), gross return (Rs. 12,84,400/ha), net return (Rs. 9,58,730.5/ha) and B:C ratio (3.94) as compared to other treatments. On an average the yield of per plant was 20-30 kg and 50- 64 tonnes per hectare. In terms of economy as per market analysis, price of banana in Jammu is approximately Rs. 20 per kg. Thus, on an average it gave Rs. 250-300 per banana plant. On the basis of market demand, approximately Rs 6.17- 9.50 lakh net return can be obtained by cultivation of one hectare of land which is alternative business for the farmers of the Jammu and Kashmir. This may leads to revolution of banana cultivation in Shivalik range of Himalaya.