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Essential oil is a concentrated hydrophobic oily fragrance liquid extracted from plants, containing easily evapourated chemical compounds at room temperature. Essential oils are extracted from the different plant parts including the flowers, leaves, stem, bark, and roots, and they are extracted from different techniques. The most preferable method of extraction is hydro distillation which is easy to use and cheap. Essential oils are used in every field of life, used in aromatherapy, and act as antifungal, antioxidant, anxiety, pain relievers, and depression.

Introduction
1.1. Definition and Significance of Natural Products
Natural products are chemical compounds or substances derived from living organisms in nature. These diverse sources, including plants, animals, fungi, and microorganisms, produce a wide range of chemical structures and functions. The significance of natural products lies in their diverse biological activities and potential applications in therapeutics, agriculture, and various industries. Natural products have been used for centuries in traditional medicine and continue to serve as the foundation for many modern pharmaceuticals, providing templates for the development of synthetic analogues and novel drugs. Their complexity and unique structural features often translate into high biological activity, making them valuable in the discovery of new therapeutic agents. Furthermore, natural products are generally perceived as safer and more environmentally friendly compared to synthetic chemicals, contributing to their appeal in numerous applications.
1.2. Historical Perspective on the Use of Natural Products
The use of natural substances has a long history, with evidence of their application in ancient civilizations such as Egypt, China, India, and Greece. Traditional medical systems, including Ayurveda, Traditional Chinese Medicine, and Indigenous practices, have utilized plants, animals, and minerals to treat various ailments. These early practices laid the foundation for the systematic investigation of natural products and their medicinal potential. One of the earliest documented uses of natural products in medicine is the Ebers Papyrus, an ancient Egyptian text from around 1550 BCE (Packard, 1931). Similarly, ancient Indian texts, such as the Charaka Samhita and the Sushruta Samhita, describe the use of plant-based medicines in Ayurvedic tradition. The scientific investigation of natural products gained momentum in the 19th century, with the isolation of pure compounds, including morphine, quinine, and penicillin.
1.3. Current Trends and Applications
Natural products are invaluable in various industries, serving as a rich source of bioactive compounds for drug development, sustainable agriculture, and functional food products. Many modern medicines, such as the anticancer drug paclitaxel and the antimalarial artemisinin, are derived from natural sources. These naturally-derived compounds often possess unique structures and mechanisms of action that are difficult to replicate synthetically.

Phenolic compounds constitute a major class of plant secondary metabolites that are widely distributed in the Plant Kingdom. Plant phenolics are biosynthesized through the shikimate/phenylpropanoid pathway leading to different compound classes. They may occur in their natural sources in free forms, as glycosylated or acylated derivatives, and as oligomeric and polymerized structures, such as hydrolyzable and condensed tannins, phlorotannins or lignins. They may also be found linked to plant matrix components such as cell walls, carbohydrates or proteins. It should be indicated that although the terms “plant phenolics” and “polyphenols” are indistinguishably used by some authors, they are not synonymous.

The quality of essential oils is very complex. An individual oil may have hundreds of ingredients, the principle components being a group of complex substances known as terpenes and their compounds or derivatives. Due to this reason a single essence has a wide range of therapeutic actions.
The chemical constituents of plants are of two kinds: products of primary metabolism and products of secondary metabolism. The first are mainly carbohydrates, amino acids and fixed oils produced by light-absorbing (photosynthetic) processes. The latter group of chemicals arises from the primary metabolites. They include glycosides, terpenoids, alkaloid and essential oils. Essential oils lack the water soluble constituents such as tannins,
bitter compounds sugar, mucilages and pectins, playing important role in the medical action of the plant.
The essential oils have been credited with possessing almost all therapeutic properties in a concentrated form. The therapeutic properties of the whole oil are that result of synergism: an interaction of all its chemical constituents working harmoniously together so that the whole essential oil becomes more potent than the sum of its individual parts.
The essential oil lacks certain chemical compounds found in the raw plant material. It is still a highly complex substance, that it cannot be replicated in the laboratory. Even though high-tech processes such as gas liquid chromatography (GLC) can separate out main constituents of essential oils. By looking at the chromatogram, it is impossible to isolate the numerous trace elements including number of unidentified compounds which make up
the whole.

Essential, volatile or ethereal oils are mixtures composed of volatile liquid and solid compounds which vary widely in regard to their composition and boiling points. Plants owe their fragrance to presence of traces of essential oils in different parts. Numerous fragrance materials are present in roots, stems, barks, leaves, flowers, fruits and heartwoods.     
Several processes like distillation, enfleurage, maceration, expression, solvent extraction and fluid extraction are available for extraction of aroma principles. Application of these processes, either singly or in combination depends upon nature of material and essential oil or absolute intended to be recovered.

Introduction

Fruits, vegetables and some non food crops are rich source of natural antioxidants, food colorants and other bioactive natural products. Nutraceuticals and natural food colorants exhibit anti-oxidant, anti-carcinogenic, anti-inflammatory, anti-bacterial and anti-proliferative properties. They tend to enhance ability of the body to fight, cure and prevent diseases. Natural anti-oxidants are particularly important because they scavenge harmful superoxide, hydroxy, and/or peroxide free radicals, and protect the body against a variety of cancers, heart and other life style related diseases. Vegetable like orange carrot, red carrot, black carrot, beet root, tomato, chilli, and fruits like pomegranate, cherries, berries, watermelon and jamun are rich source of natural antioxidants and food colorants. While tomato and red carrot are rich in lycopenes, and carotenes; black carrot is a major source of stable acylated anthocyanins. The dark red colour of beet root is associated with anthocyanin, betacyanin and betaxanthins. Similarly, the red colour of chilli is attributed to the carotenoid pigment capsanthin. Capsaicinoids in chilli are responsible for the hot flavour and anti-inflammatory properties. Antioxidant and other beneficial health effects of pomegranate, cherries, berries, etc. have been attributed to anthocyanins and related polyphenols. Jamun, an under-utilized tropical fruits is rich in anthocyanins and flavonoides. Like anthocyanins, flavonoides and carotenes, lycopene helps to prevent various types of cancer particularly the prostate cancer.

Microalgae represent a promising alternative to renewable source of feedstock for natural food colorants and nutraceuticals and functional food production. With over 40,000 identified species, microalgae are one of the most diverse groups of organisms on earth. They naturally produce large quantities of many biomaterials. Out of the diverse compounds produced by microalgae, the algal pigments such as phycobillins, carotenoids and xanthophylls have gained commercial importance owing to their unique and multiple applications as food and cosmetic colourants, antioxidants and several beneficial effects of nutraceutical and pharmaceutical value. Microalgal research over the years has geared up significantly from outdoor mass culture in algal ponds, refinement of the cultivation process and life cycle assessment, downstream processing of biomass (i.e., harvesting, dewatering, and drying), to extraction, to systems/process scale-up analysis and development of an economic feasibility model for commercialization of algal biomaterials with nutraceutical properties. Among the various microalgae, Spirulina and Haematococcus are the major source of naturally occurring biopigments which are gaining importance as natural pigments in food, drug and cosmetic industries as an alternative to currently used synthetic colour. Improvement in the cultivation methodologies and use of closed photobioreactors of different configurations, fermentation reactors, open reactors and tubular reactors have opened up new vistas for the development of microalgal biotechnology and bioprospecting. The main constraints in non-exploitation for mass cultivation of microalgae are their slow growth rate, low biomass yield and inadequate culture methodologies and these issues remain to be addressed.

ABSTRACT

Zingiber officinale is a herbaceous perennial. The absence of seed set in ginger makes conventional breeding methods inapplicable. Genetic modification through biotechnological means is the only way for introducing novel genes into the cultivated types. Transient expression of the gus reporter gene was used in preliminary experiments. Agrobacteruim tumefaciens strains EHA105/p35SGUS Int. effective in expressing b- glucuronidase activity was used for standardizing the optimum conditions for effecting transformation, such as preculture of explants, bacterial dilution, infection time, co-cultivation period etc. In addition, effects of acetosyringone and explant wounding were evaluated using the same strain. Genetic transformation of bud and regenerated plantlets was confirmed by their ability to grow on MS medium containing kanamycin 100 mg l-1 + cefotaxime 300 mg l-1 by histochemical b-glucuronidase assays.

Benefit of biofertilizers have been extensively studied for various agricultural and horticultural crops. However, the effectiveness of inoculated biofertilizers are influenced by several factors. If the use of biofertilizers have to be popularized in horticultural crops, the major factors affecting their performance particularly in relation to soil, plant, quality of the product and application methods have to be addressed. Factors affecting the performance of biofertilizers are:

4.1 Quality of the product

4.1.1 Production process

The high quality of biofertilizers are determined by the production process (Bashan et al., 2014). At the same time, the population density of mother culture and the quality of the final products are directly related (Stephens and Raskm, 2000). As the inoculum consists of a strain, the relationship among the microorganisms interaction in the rhizosphere have to be understood. In the case of more than one microorganisms, the efficiency depends on the various mode of action by various microorganisms which might overlap with antagonistic acitivities (Vassilev et al., 2001, 2006 b). The relationship of microorganisms with plants is not based on the taxonomic diversity, but their functional diversity (Maherali and Klironomos, 2007).

The efficiency of biofertilizers could be assured by enhanced shelf life of the inoculant and biological traits which are a challenge for developing a formulation (Bashan, et al., 2014). Hence, either single or consortia of microorganisms formulation with or without additives determines the efficacy of the biofertilizers. The formulation determines shelf-life during storage and transport, which enhances the persistence of the strain in soil and get maximum benefits to the host plants after inoculation (Manikandan et al., 2010; Schoebitz et al., 2012). Different kinds of carriers are used in the formulation process which affects the overall quality and efficacy of the biofertilizers (Bashan et al., 2014; Herrmann and Lesueur, 2013). Granular inoculants showed good results under stressed soil conditions (Clayton et al., 2004; Lupwayi et al., 2006). Encapsulation of the microbial strain into polymers allow diverse compositions and structures (Vassilev et al., 2005) but, have limitations for commercial production (Bashan, et al., 2014; John et al., 2011). Use of additives might improve the shelf life of the product (Bashan et al., 2014; Malusà et al., 2012; Herrmann and Lesueur, 2013) with introduction of specific substances to enhance the efficacy of biofertilizers.

1. Introduction

The status of mechanization in India is at a very low level for horticultural crops. The farmers usually go behind the use of traditional methods for different farm operations (Fig 1) and practices for cultivation of horticultural crops, their handling, storage, and processing. This results in less yields, high cost of cultivation and high crop losses from harvesting till processing. The use of different available hand tools, machinery and hi-tech technologies in the production and processing of the crops will lead to increased yields and decrease in losses to a great extent (Mandhar, 1999).

Introduction
Spices have been considered significant in the culinary art from time immemorial. India, “The Homeland of Spices” is one among the largest producers, consumers and exporters of spices as well as spice products. Spices comprise different plant components such as floral parts or fruits or berries or seeds or rhizomes or roots or leaves or kernel or aril or bark or bulbs. The recorded history of the use of spices dates back to 5000 years or so from the Egyptian or at the most from the Indus valley civilization. Spices are used for flavouring, seasoning and imparting aroma in various cuisines. Besides these inherent qualities, some of them are known to be fungistatic, antimicrobial or antibiotic. They are also processed into numerous ‘value added’ products such as oleoresins, essential oils, curry powder etc. Black pepper, cardamom and turmeric are the major spice crops and its production and marketing are the main economy stays of lakhs of people of the country. Billions of rupees are involved in domestic trading and foreign export. Therefore, it is highly imperative for farmers to adopt the advanced practices to sustain spices productivity. Even, additional income could be generated from the intercropping of spices from the interspaces in mango orchards (Shukla and Adak, 2020). Thus the present chapter included all aspects of production to protection and value addition for  the benefits of growers.

Lipids are a class of compounds that are defined by how they are isolated, rather than by their composition. Lipids are molecules that can be extracted from plants and animals by low polarity solvents such as ether, chloroform, or even acetone. They are not appreciably soluble in water. Fats and the fatty acids from which they are made belong to this group as do oils, waxes and steroids. Hydrolyzable lipids are those that contain a functional group that will react with water. The functional group is usually an ester and the list of compounds includes neutral fats, waxes, phospholipids, and glycolipids. Non- hydrolyzable lipids lack such functional groups and include steroids and fat-soluble vitamins (e.g. A, D, E, and K).

Fats and oils are composed of triacylglycerols. These are compounds prepared by the union of glycerol (1,2, 3-tri- hydroxypropane) and 3 fatty acids to form a triester. Fats and oils belong to a group of biological substances called lipids. Lipids are biological chemicals that do not dissolve in water. They serve a variety of functions in organisms, such as regulatory messengers (hormones), structural components of membranes, and as energy storehouses. Fats and oils generally function in the latter capacity. Fats differ from oils only in that they are solid at room temperature, while oils are liquid. Fats and oils share a common molecular structure. The fats and oils contain three ester functional groups. Fats and oils are esters of the tri- alcohol, glycerol (or glycerine). Therefore, fats and oils are commonly called triglycerides, although a more accurate name is triacylglycerols. Triglyceride molecules contain mostly carbon and hydrogen atoms, with only six oxygen atoms per molecule. This means that fats and oils are highly reduced (un-oxidized). Oils are particularly common in seeds, where the stored energy helps seedlings during germination, until they can exploit solar energy through photosynthesis. Fatty acids contain an even number of carbon atoms, from 4 to 36, bonded in an unbranched chain. Most of the bonds between carbon atoms are single bonds. If all of these bonds are single bonds, the fatty acid is said to be saturated, because the number of atoms attached to each carbon atom is the maximum of four. If some of the bonds between carbon atoms are double bonds, then the fatty acid is unsaturated. When there is only one double bond, it is usually between the 9th and 10th carbon atom in the chain, where the carbon atom attached to the oxygen atoms is counted as the first carbon atom. If there is a second double bond, it usually occurs between the 12th and 13th carbon atoms, while a third is usually between the 15th and 16th.

English name : Fennel
Botanical name : Foeniculum vulgare Miller
Family : Apiaceae
Chromosome number : 2n = 22
Centre of origin : Southern Europe, Asia and Africa
Fennel (Foeniculum vulgare Miller), commonly known as Saunf or Badi Saunf, belongs to the Apiaceae family and is primarily cultivated for its seeds, from which fennel oil is extracted. The main component of fennel oil from cultivated F. vulgare is trans-anethole, which typically makes up 50-60% of the oil. Other constituents include d-2 fenchone, methyl chavicol, 2-pinene, camphene, d-2 phellandrene, dipentenes, B-pinene, terpinene, limonene, 1,8-cineole, a-terpinene, linalool, cis-anethole, anisic aldehyde, anisic ketone, and foenicullin. Recently, the presence of a toxic ketonic constituent has been reported. Indian fennel oil contains over 70% anethole and around 6% fenchone. Sweet or Roman fennel fruits are rich in anethole, phellandrene, and d-limonene, with a high concentration of anethole (up to 90%) and the absence of fenchone contributing to its sweet fragrance and flavour. Anisaldehyde and anisic acid have also been identified as present.

1.0 INTRODUCTION

The fennel (Foeniculum vulgare Mill.) is important seed spices belonging to family Apiaceae, and a native of Southern Europe and Mediterranean area. Later it spread to the far East and far north in Europe. To the ancient Greeks, fennel represented success and so it was called “Marathon” in reference to the battle, fought in fennel field where the Greeks defeated the Persians in 490 B.C. Fennel was also a symbol of success to the Romans. During the thirteenth century in England, fennel was considered as a royal spice and was served to kings with fruits, bread and pickled fish seasoned with fennel seeds. Fennel was even described by Longfellow in his poem The Goblet of Life. According to early Sanskrit writings, fennel has been described as Madhurika and was known as far back as 2000 BC for its cultivation in India.

Thrips : Thrips flavus : Thripidae

Infestation of thrips is generally during hot months with high night humidity.  The attack by thrips leads to discolouration, bronzing and drying of affected leaves.

Semilooper : Thysanoplusia orichalcea  : Noctuidae

Bluish green or grey coloured eggs are laid singly on lower surface of leaves.  Larvae feed voraciously on leaves and defoliate plants.

2.1. Origin and distribution

• Native to South Eastern Europe, West Asia and Mediterranean region.

• Cultivated in Central and Southeastern Europe, Western Asia, India, and Northern Africa.

• In India, cultivated in Rajasthan, Madhya Pradesh, Gujarat, Uttar Pradesh, Maharashtra and Punjab.

2.2. Economic importance

• Culinary use in smaller quantities.

• Used as an herb (Dried or fresh leaves), spice (Seeds) and vegetable (Fresh leaves, sprouts, and micro greens).

• Seeds are used both whole and powdered in the preparation of pickles, vegetable dishes, dal and spice mixes such as panch phoron and sambar powder.

• Balance cholesterol.

• Soothe upset stomach and digestive problems.

• Reduce menstrual cramps.

• Seeds are aphrodisiac effect.

• Reduce appetite.

English name : Fennel
Botanical name : Foeniculum vulgare Miller
Family : Apiaceae
Chromosome number : 2n = 22
Centre of origin : Southern Europe, Asia and Africa
Fennel (Foeniculum vulgare Miller), commonly known as Saunf or Badi Saunf, belongs to the Apiaceae family and is primarily cultivated for its seeds, from which fennel oil is extracted. The main component of fennel oil from cultivated F. vulgare is trans-anethole, which typically makes up 50-60% of the oil. Other constituents include d-2 fenchone, methyl chavicol, 2-pinene, camphene, d-2 phellandrene, dipentenes, B-pinene, terpinene, limonene, 1,8-cineole, a-terpinene, linalool, cis-anethole, anisic aldehyde, anisic ketone, and foenicullin. Recently, the presence of a toxic ketonic constituent has been reported. Indian fennel oil contains over 70% anethole and around 6% fenchone. Sweet or Roman fennel fruits are rich in anethole, phellandrene, and d-limonene, with a high concentration of anethole (up to 90%) and the absence of fenchone contributing to its sweet fragrance and flavour. Anisaldehyde and anisic acid have also been identified as present.

1.0 INTRODUCTION

Fenugreek is one of the oldest cultivated spice crops of the world and grown for its medicinal value and forage in India, Western Asia and Nile valley since remote antiquity. In Egypt, it has been cultivated since 1000 BC and is part of the Indian diet for over 3000 years. Fenugreek is used as an important component of holy smoke in Egyptian incense. It is found growing wild in parts of northern India and cultivated all over the subcontinent for its green leaves and seeds. Fenugreek is a native of south-eastern Europe and Western Asia.  India is also said to be a native of fenugreek (Shanmugavelu et al., 2002) and found growing wild in Kashmir, Punjab and upper Gangetic plains.

Introduction

India is the second most populous country in the world. The per capita cultivable land resource is shrinking day by day. To meet the food, fibre, fuel, fodder, and other needs of the growing population, the productivity of agricultural land has necessarily to be increased. It requires increased use of agricultural inputs like quality seeds, fertilizers, water, agro-chemicals, etc. in such a manner that do not create any environmental problems.

Plant needs different chemical elements to grow and thrive. The most important chemical elements include (i) carbon, hydrogen and oxygen, which are basic life building blocks and are available in plenty from air and water (ii) nitrogen, phosphorus and potassium which are the macro nutrients and affect the plant growth significantly (iii) sulfur, calcium, and magnesium as secondary nutrients and (iv) boron, chloride, copper, iron, manganese, molybdenum and zinc as micronutrients in very small quantities.

Fertilizer is a substance of natural or synthetic origin, applied to soils or to plant tissues (usually leaves) for the supply of one or more nutrients essential to the plant. It is classified in two types, inorganic fertilizers and organic fertilizers. Both the fertilizers play major role to the crops if they are properly and appropriately applied. To obtain maximum benefits, it is important to apply fertilizers at an appropriate time, amount and in a precise manner. The method of fertilizer application should be selected appropriately taking into consideration the age and stage of crop plants, their root extensibility, type of orchard management, kind and amount of fertilizers to be applied. The fertilizers should be given over the area of crop plants, where their active roots are spread. In fruit trees, fertilizer should be given in restricted area i.e. in the surrounding area of about 1 to 1.5 m away from the trunk of the trees in active root zone.

When to apply the fertilizers?

Majority of the crops require nutrients at different stages like growth & development, flowering and fruit setting time. In fruit crops, stages like new flushes emergence, floral buds differentiation and fruit development require more nutrients. Time of application of fertilizers may also vary depending on types of fertilizers, need of the crops, soil and climate.