eChapters

1. Sigatoka
Diagnostic Symptoms
• Early symptoms appear on the young leaves.
• Small spindle shaped spots on foliage with greyish centreand yellowish halo running parallel to veins.
• Spots are mostly seen along the edge of the leaf with defined margin and possess dark brown to black margin. Spots coalesce and whole leaf blade dries up.
• On the upper surface of the spots, fructificationsof the fungus appear as black specks unevenly and individual bananas appear undersized and their flesh develops a buff pinkish colour, and store poorly (Fig. 1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Eumycota
Sub- division : Deuteromycotina
 

Pseudostem borer: Odoiporus longicollis: Curculionidae

Distribution : It is widely distributed in all banana growing areas and has got introduced into South India somewhere in the eighties and spread fast in the banana areas of Tamil Nadu, Maharashtra, Kerala and Karnataka.

Biology : Adults of the weevil are reddish brown to black and resemble the adults of the rhizome weevil in size and shape. They live for about four months. The snout is long with the characteristic geniculate antennae.

Banana, Musa paradisiaca is one of the most important tropical fruit crops. India tops in the production of banana in the world and almost throughout the year banana fruit is available form one or another part of our country. Maharashtra, Tamil Nadu and Karnataka are the major states in cultivating and producing banana in India. The major insect pests associated with banana crop are described hereunder:

Banana weevil, Cosmopolites sordidus (Germar) (Curculionidae: Coleoptera)

It is most destructive and specific pest of banana and is distributed throughout the country. The eggs are elongate, oval (about 2 mm long), pearly white and present on upper surface and crevices of rhizome. The grubs are creamy white, stout, fleshy swollen in the middle and has red head capsule (Plate 10a). The full grown grub measures 15 mm in length. Pupae are white and found in the pupal chamber made in the periphery of rhizome. The newly emerged weevil is shiny reddish brown but soon becomes uniformly black (12.5mm in length). Longitudinal striations are found in the elytra and it does

Basella (Basella alba syn Basella rubra) is also known as Poi, Ceylon spinach, Malabar spinach, Indian spinach or vine spinach. It is a popular leaf vegetable grown in almost all parts of India and widely grown in North-East and South India. Besides India, it is widely cultivated in tropical Asia and Africa. It is a climbing perennial plant, but is sometimes cultivated as an annual vegetable. The plant is succulent with tender leaves. It is a fast growing, productive leaf vegetable for home gardens and market growers. 

Composition
Young shoots and leaves of Basella are a rich source of calcium, iron, and Vitamins A and C.

Uses
Fresh tender leaves and stems are consumed as leaf vegetable after cooking. It is preferable to cook the leaves in soups and stews as half of soluble solids may be lost by boiling in water. Red cultivars contain a colouring matter in leaves, stems and ripe fruits reported as a dye for colouring food. Because of its mucilaginous nature of leaves and stems, it is used as poultice. Juice of leaves is prescribed against constipation, especially for children and pregnant women (Burkill, 1935).

Statistics involves procedures for collection, classification, analysis, interpretation and presentation of numerical data (Gupta, 1997). Statistical investigations involves four stages ie. collection of data, compilation of data, analysis of data and drawing inferences. For any statistical analysis, first step is data collection which constitutes the foundation for statistical analysis. The required data may be obtained either through survey or an experiment. Survey is the process of collecting data from a population but have no control over the factors which may affect the population characteristics under study, whereas in experiment, data is collected while control is exercised over the factors which may affect the population characteristics under study. Experiments always and almost provide better information than the survey but surveys are necessary in natural populations. When data is not available, it is collected by any of the two methods i.e. census or survey method. In census method, data are collected for each and every unit of the population whereas in sample method a representative sample is drawn from the population on the basis of which all the conclusions are drawn about the population from that sample.

India is a vast country with varied soil and agro-climatic conditions and therefore is able to grow a wide range of vegetables. In view of their productivity, diversification, nutrition, value addition and export, vegetables are an important component in Indian agriculture. Up to 1939, vegetable seeds were used to be imported from Australia, U.K., U.S.A., but after World War I and II, India started seed production of vegetables. In the recent past, the seed industry has made rapid strides over the past three decades and has contributed significantly to the quantum jump in agricultural production and India emerged as the second largest producer of vegetables, next to China in the world. During 2005-2006, India produced 113.5 million t of vegetables from 7.2 million ha with a productivity of 15.76 t/ha. The seed industry has increased certified and quality seed production and distribution by the organized sector to about 60 lakh quintal from 300q in 1964-65. India can be proud of having launched and managed both crop improvement and seed improvement as simultaneous and parallel programmes playing complimentary and synergistic roles.

Flow Cytometry is a tool used to detect and measure chemicophysical characteristics of a population of cellular particles. Modern day biomedical research needs the molecular assembly of tools to proceed in the advent of biomedical research with real time diagnostic, prognostic and experimental detection of markers. For the same, a naïve tool is flow cytometry. In this technique, a sample having cells or particles is suspended into a fluid and injected into instrument. The ability to perform these measurements in a very rapid time span is one of the key advantages of the flow cytometry process. A single-cell suspension must first be prepared in order to analyse solid Tissues. A flow cytometer is look alike to microscope, except that, instead of producing an image of the cell, it generally offers a cell-by-cell basis high-throughput, automated quantification of specified optical parameters.

They can quantify up to three to six properties or components are quantitated in a single sample, cell by cell, for about 10,000 cells, in less than one minute. Sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. These cells are tagged with fluorescent markers in order to absorb the light and then emitted them in the form of band of wavelengths. Average ten thousand cells can be quickly examined and the data gathered are processed by a computer.

1) Anthracnose
Diagnostic Symptoms
• All the above ground parts are affected. However, the characteristic symptoms appear on pods.
• On cotyledons, spots are sunken dark brown or black with pink spore mass.
• Seedling infection results in collapse of seedling.
• Spots on leaves appear on lower side and are black. Later these may also appear on upper surface. When the infection is severe, the affected plants wither off.
• Black, sunken, circular spots of varying sizes appear on pods with bright red, yellow or orange margins. The centre of these spots later turns grey or pink due to sporulation of the pathogen. The border of these spots appear raised (Fig.1).

Bearing

Bearing is connotation of flowering and fruiting. When a fruit tree flowers and fruits, it is said in bearing condition. Different fruits have different bearing habits, inflorescences, types of flower, mode and medium of pollination, pollen viability and all these account a lot for the bearing of fruit plants.

Bearing habit

If one observes a mango tree, he/she may find it does produce the fruits terminally on a shoot and it citrus he/she finds the fruits always produced laterally in the leaf axils. The manner or patter in which a plant produces its fruit is known as bearing habit or fruiting habit. Bearing habits are specific to the kind of plant. In determining the bearing habit of any plant three points are to be considered.

In general, plants show two distinctive phases; i.e. vegetative growth and reproductive growth. The primary objective of plant species is to reproduce themselves through the reproductive phase. However, some of the plant species do not produce viable seeds, but reproduce themselves in nature by other means. Flowering and fruiting habits vary with plant species and are governed by the kind of plant species and its growth habit, environment etc. In general, flowering and fruiting habits are known as bearing habits and it can be described by the location and type of buds, which produce flowers and fruit. The relative position of fruit with reference to its potential bud-giving rise to flower or inflorescence on the shoot is known as bearing habit. Each plant has its own bearing habit and are either terminally, laterally or adventiously Terminally means the fruit buds are formed on long or short growth, while laterally means the fruit buds are formed in the axils of present or past season’s growth. Adventiously, the fruit buds are formed at any point on the trunk. On the basis of bearing habits, fruit crops are categorised into the following eight groups

By the time a child reaches the age of two he can usually keep himself dry during the day. However, it may be another year or more before he can keep dry all through the night. Even then, the best-trained child may fail to live up to expectations on certain occasions when he is highly excited.

Older boys and girls may also have occasional problems at night, especially when sleeping in a strange bed after a highly exciting evening.

The bladder is emptied unconsciously especially during sleep at night; may be associated with enlarged tonsils, thyroid deficiency, phimosis, intestinal worms, blanitis or vulvitis also sometimes pyelitis, cystitis, oxaluria even minor epilepsy.

There are many causes for this condition, ranging all the way from simple neglect of the calls of nature to degenerative changes in the spinal cord. The condition is found in all classes and at all ages. Nocturnal bedwetting is very common in weak, neurotic children. The next most numerous class is that of women who have born children and who have passed the fiftieth milestone. A laugh, a sneeze, a fright and the urine spurt from the bladder. Probably this is want of proper adjustment of the function of the nervous mechanism of the sphincter and the detrusor and the former fails to inhibit the later.

Scientific name: Atropa belladonna L.
Family: Solanaceae
Belladonna, also known as deadly nightshade, is a legendary Central European nightshade with a rich history. The plant contains hallucinogenic alkaloids, primarily atropine, which is named after the plant.
Origin and distribution
Atropa belladonna is native to temperate southern, Central, and Eastern Europe, as well as North Africa, Turkey, Iran, and the Caucasus. It has been naturalized in some areas of North America and is considered a weed species in regions with disturbed soils.
Botany
Belladonna, is a plant species characterized by its distinctive features. It grows to a height of approximately 1.5 meters, with a purplish-colored stem. The root system is thick, fleshy, whitish, and branched. The plant's leaves are simple, dark greenish in color, ovate, and alternate in nature. The flowers are solitary and bell-shaped, blooming from June to September. The plant produces berries that are highly toxic, and the seeds contain toxic alkaloids.

The word Belladonna is derived from two Latin words Bella means fine or beautiful and Donna means lady. Its generic name ' ATROPA' comes from the Greek fate ATROPOS, 'THE INFLEXIBLE ONE'.

Think of Belladonna if you see a red surface because this medicine is associated with red, blood shot eyes, red, puffed face, red, swollen throat, red skin, which radiates heat. This redness indicates inflammation. Thus a large number of acute inflammatory diseases respond well to Belladonna.

Belladonna problems come on suddenly, with violence, run a regular course and then disappear as suddenly as they come.

When an inflammation is localized, Belladonna is the remedy in the first stage. Such inflammation comes suddenly, is red, painful and throbbing. This can be in the head, throat, breast or anywhere else in the body. This can be in the form of a carbuncle or a boil, on a hand or on a foot. Belladonna controls and aborts the problem in its first stage.

Endophytic Microorganisms

Endophytes (‘endo’, inside; ‘phyte’, plant) include microorganisms that thrive inside the plant tissues without any obvious adverse effects on the host, and they include mainly bacteria and fungi (Hallmann et al., 1997; Bacon et al., 2002; Hardoim, 2015). Endophytic microorganisms share an intimate association with the host adding essential and functional capabilities. Therefore, plant may be considered as a superorganism composed of an amalgam of both selected symbiont microbiota and host cells (Hardoim, 2015) with the new concept of holobiome, i.e., plant together with the associated microorganisms collectively governing the plant activities (Hardoim et al., 2015; Vandenkoornhuys et al., 2015).

Ber (Zizyphus) is egg-shaped fruit of orange or brown colour, with edible acid pulp and hard central stone. The Ber, Indian jujube, Z. mauritiana Lam. (syn. Z. jujuba L.) is adapted to warm climates. It belongs to the family Rhamnaceae. It is often called merely jujube, or Chinese date, which leads to confusion with the hardier species. Other English names are Indian Plum, Indian cherry and Malay jujube. In India, there are 90 or more cultivars differing in the habit of the tree, leaf shape, fruit form, size, color, flavor, keeping quality, and fruiting season (Morton, 1987). Ber (Zizyphus pss.) is one of the hardiest fruit trees cultivated in India. It is an ideal fruit crop for arid and semi-arid regions and even for waste lands. Ber has been recognized as a useful edible fruit since mythology of Ram and Shabari in India and depicted in Ramayana. Researchers mention both Z. mauritiana and Z. jujuba and even the wild Z. nummularia.

Cultivars: Umran, Banarasi Karka, Seo, Gola.

Uses

The Jujube is classed with the raisin, date, and fig as a pectoral fruit, being nutritive and demulcent. It is eaten both fresh and dried. Bark is used for tanning. Leaves are laxative and used in throat trouble. Leaves are also used as a good fooder particularly in arid zones. The tree serves as a host for lac insects. Wood is used for making agricultural implements and tool handles. The preserved products made of ber fruit are murabba., candy, diced ber, dehydrated ber, ber pulp, ber jam etc.

Ber fruit fly:  Carpomyia vesuviana :Tephritidae

Adult is a small fly with black spots on the thorax and dark spots on the wings. They have only one pair of wings typical of diptera. The attack by the insect causes rotting of fruits and fruit drop. The adult female lays eggs singly in cavities made on the fruit by the ovipositor.  One female can lay up to 22 eggs. Egg period lasts for 2-3 days and the emerging maggots feed inside the fruit for about 7-10 days. The fully grown maggot falls to the ground to pupate in soil.  Pupal period is around 5-30 days. There can be 2-3 generations /year.

Ber (Chinese date, Chinese fig or bore) (Ziziphus xylopyrus) is an important minor fruit of India. It is a hardy fruit tree cultivated all over India and also known as poor man’s fruit. It is widely cultivated in Punjab, Haryana, Rajasthan, Uttar Pradesh, Madhya Pradesh, Bihar, Gujarat and Maharashtra. Ber does well even under marginal growing conditions and provides quality yields at low cost (Shukla, 1996). Fruits are eaten fresh but the other forms like dried, candied and squash can also be prepared from ber. Stem bark, root and leaves have medicinal values. Leaves of ber are used as fodder in dry regions. Ber tree is a host plant for rearing the Tachardia laccad, a lac insect (Sharma and Kore, 1990).

Ber, Ziziphus mauritiana is indigenous to India and was called as poor man’s fruit earlier. But now it has become a good source of income to the orchardists, in the arid and semi-arid regions of the country, mainly with the development of new varieties. A number of insect pests (around 150) infest the ber tree but only few are the major ones, the description of which is provided below:

Fruit fly, Carpomya vesuviana Costa (Tephritidae: Diptera)

It is a major pest of ber in all the ber growing regions and proves as a bottleneck in its production and productivity. It is monophagous and develops only in fruits of Ziziphus spp. Most of the cultivated varieties are susceptible to this direct pest. The eggs are small, elongate, spindle shaped and creamy white. Maggots are creamy white (Plate 9a) and slightly smaller than maggots of other fruit flies. Pupae are barrel shaped and brown. Adults are smaller than housefly, brownish yellow with brown longitudinal stripes on the thorax. This fruit fly can be distinguished easily from other species due to distinctive pattern of black marks on the scutum and scutellum (Plate 9a). Wings are hyaline with four yellow cross bands.

Revolutionizing skin cancer detection
The diagnostic process for skin cancer is evolving. Physicians have in the past relied upon physical exams to be followed by scanning and biopsies taking anywhere between 15 days to return results. Although most abnormalities turn out to be non-cancerous, this is distressing to patients and contributes to healthcare costs. Additionally, certain growths that turn out to be cancerous remain undiagnosed since they have not been visible.
Breakthrough startup Damae Medical is set to reverse this process. The startup has developed an advanced probe that is able to take 3D images of the skin to unveil the full extent of abnormalities in real time. This enables doctors to diagnose in an instant, enabling improved personalized treatment. When an operation is required only diseased tissue is removed to prevent unnecessary treatments and improved outcomes in patients.
The Origins in the Institut d’Optique
The beginnings of Damae Medical can be traced to Palaiseau-based Institut d’Optique where in 2013 two photonics students envisaged potential in an emerging imaging tech. Together with the inventor himself in 2014, they cofounded Damae Medical by taking an exclusive license to the core family of patents held by three partner institutions. While this contract was beneficial in launching the company to an early growth spurt, in the long run created difficulty in exercising control. This was resolved by taking an equity-forcontrol purchase in 2019 by Damae to acquire the foundational patent

Scientific name: Piper betle L.
Family: Piperaceae
The betel vine, scientifically known as Piper betle L., is a perennial climber native to Southeast Asia, including India. It belongs to the Piperaceae family. The betel vine has been an integral part of Indian culture, tradition, and medicine for thousands of years.
In Hinduism, the betel vine is considered a sacred plant, often offered to gods and goddesses. The leaves are used in various rituals, including weddings and pujas (prayer ceremonies). In Indian tradition, betel leaves are also offered as a mark of respect and hospitality to guests.
Betel leaves have been used in Ayurvedic medicine for their numerous health benefits. They are rich in antioxidants, anti-inflammatory compounds, and essential oils. The leaves are used to treat various ailments, including digestive issues, respiratory problems, skin conditions, and oral health issues.
In addition to their medicinal properties, betel leaves are also used as a mouth and breathe freshener. The leaves are chewed with betel nuts (areca nuts) and lime, a practice known as "paan" in India. The betel vine is a versatile plant with significant cultural, medicinal, and economic importance in India and other parts of Southeast Asia.

8.1. Origin and distribution

• Originated in South and South East Asia.

• Cultivated mostly from Pakistan to Papua New Guinea.

8.2. Economic importance

• In India and Sri Lanka, sheaf of betel leaves are traditionally offered as a mark of respect and auspicious beginnings like greeting elders at wedding ceremonies, celebrating the New Year, and offering payment to Ayurvedic Physicians and Astrologers (To whom money and/or areca nut placed on top of the sheaf of leaves are offered in thanks for blessings).

• Consumed in Asia as betel quid or paan with arecanut and/or tobacco.

• Used in cooking usually raw for its peppery taste.

• Medicinal use over a 300 years history in China.

Botanical Name : Piper betel
Family : Piperaceae
Origin : Malaysia.
Chromosome No. : 2n=2x=26 
Useable Part : Leaf 
    ·    Betelvine leaves have a strong pungent aromatic flovour and are widely used as masticatory. 

Scale insect: Lepidosaphes sp.: Diaspididae

The mature scale is elongated and oval in shape and dark grey in colour. They infest the stems, leaves and petioles, causing shriveled leaves due to sucking of the sap. The affected parts wither and dry up. In severe infestation leaves dry up and give sticky look and gradually the vine wilts.

1. Vein-Clearing/Yellow Vein Mosaic
Bhendi yellow vein mosaic virus
Diagnostic Symptoms
• Yellowing of the entire network of veins in the leaf blade is the characteristic symptom.
• In severe infections, the younger leaves turn yellow, become reduced in size and the plant is highly stunted.
• The veins of the leaves will be cleared by the virus and intervenal area becomes completely yellow or white.
• In a field, most of the plants may be diseased and the infection may start at any stage of plant growth.
• Infection restricts flowering and fruits, if formed, may be smaller and harder.
• The affected plants produce fruits with yellow or white colour and they are not fit for marketing (Fig.1).
• The virus is spread by whitefly.

Shoot and fruit borer: Earias vitella (=E.fabia), E. insulana: Noctuidae

This is a pest on cotton, bhendi, hibiscus, holly hock and other malvaceous vegetables. The damage is due to the larva that bores into tender terminal shoots in the vegetative stage and flower buds;  flowers and young fruits in the fruit formation stage.  The damaged shoot drops, wither and dry up.  The infested fruits have bore holes, are deformed and become unfit for consumption.  Bore holes are plugged with excreta.

Introduction

Antioxidants are substances, which retard or prevent deterioration, damage or destruction caused by oxidation. Fortunately, the body has an army of antioxidants for damage limitation. Antioxidant form an integral part of the nutraceutical market. During the last few years’ research has confirmed that many have the common disease and ailments of 21^st century (Coronary Vascular Disease, diabetes, cataracts, high blood pressure, infertilities, respiratory infection, and rheumatoid arthritis) are associated with tissue deficiency and/or low dietary levels of compounds called antioxidants. The oxygen is consumed in the body during metabolism by process called oxidation. During oxidation free radicals are generated. These free radicals at a molecular level burn everything they touch. Antioxidants are power full electron donors and react with free radicals which damage the biomolecules. The formed antioxidant radical is stable and non-reactive. Antioxidants are quite large in number and diverse in nature which oppose the process of oxidation largely by neutralizing free radicals at relatively small concentrations and also have the potential to inhibit the oxidant chain reactions.

Introduction

Khejri (Prosopis cineraria) grows in dry and arid regions of Arabia and in regions of India mainly Rajasthan, Haryana, Punjab, Gujarat, Western Uttar Pradesh and drier parts of Deccan. The tree is locally known as Jandi or Khejri (India), Jand (Pakistan) and Ghaf (Arabic). It is the State tree of Rajasthan, India. Prosopis cineraria are small to medium size evergreen thorny tree. It is also known as “wonder tree” and “king of desert” as all the parts of tree are useful. The importance of the medicinal value of this tree has been highlighted in our ancient literature. The bark of the tree has abortifacient and laxative properties and is also used as a remedy for rheumatism in the central provinces. The leaves are of high nutritive value and locally called ‘’Loong’’. Leaf paste of P. cineraria is applied on boils and blisters, including mouth ulcers in livestock. The smoke of the leaves is considered good for eye troubles. Leaf extracts of P. cineraria have been reported to show antibacterial, antihyperglycemic, antihyperlipidemic and antioxidative activities . Pods are brown to chocolate in colour on ripening, each containing several seeds embedded in sweet dry yellow pulp. P. cineraria pods are locally called ‘’sangar’’ or ‘’sangri’’. The pod is considered astringent in Punjab. Sangri pods are known to prevent protein and mineral deficiency. Cooked pods of Khejri are used as a functional food in Rajasthan, for the amelioration of numerous illnesses. Pods of khejri have been studied for various phytochemicals like alkaloids, saponins, tannins, flavanoids, flavanols and total phenolics. As this plant is found in water stress (or deficient area) so antioxidant potential of pods has also been investigated. Acetone and methanol extracts of Sangri pods are the most potent bioactive extracts. Nutritional analysis shows it as a good source of proteins and minerals like calcium, sodium and potassium. This study demonstrates that P. cineraria pods may be employed as nutraceutical food with rich nutrition, disease prevention and health promoting effects.

ABSTRACT

In the present investigation on three different plant species from pollution and control area were carried act for biochemical analysis such as total chlorophyll content, leaf extract pH, relative water content and ascorbic acid test. The results indicate accordance to order of tolerance index of plant species in air pollution are Macrosolen cochinchinensis, Loranthus parasiticus and Viscum album. The changes in pollution tolerance index are biochemically induced due to the surrounding environmental conditions. Thus, it is observed that Macrosolen cochinchinensis is highly tolerant species among the selected three species. Thus, in future this plant species may play an important role in pollution indicator. APTI analysis show the pollution tolerating decreasing order for there species can be Macrosolen cochinchinensis> Loranthus parasiticus>Viscum album and as ascorbic acid result indicate that Macrosolen cochinchinensis show mostly defence mechanism and highly tolerance level against different kind of pollutant. Besides these three hemiparasite plant species have some valuable ethnomedicinal important. In India tribal people used leaf and stem bark in curing abortion, miscarriage, vaginal bleeding during pregnancy, treat circulatory and respiratory system problems.

ABSTRACT

Brinjal is an important solanaceous vegetable, which holds coveted position among the different vegetables. Developing hybrids with high yield and resistance to borer would be highly beneficial. The biochemical parameters are used to study the mechanism of resistance to pests and diseases. With this aim, the biochemical constituents were estimated in five intervarietal hybrids and their parents with susceptible variety. The study on biochemical basis of resistance to shoot and fruit borer showed that among the parents of intervarietal crosses, EP 65 had the highest level of peroxidase, polyphenol oxidase and total phenol content in both shoot and fruit and it also recorded high content of solasodine next to APAU Bagmathi. Among the hybrids, the cross EP 65 x Pusa Uttam had the highest level of peroxidase and polyphenol oxidase activity in both shoot and fruit and high level of total phenols and solasodine content next to EP 104 x APAU Bagmathi . The susceptible check (CO 2) registered the lowest value for all the biochemical constituents .The study revealed that the genotypes with high or moderate level of these biochemical constituents suffered less for shoot and fruit borer infestation.

The native gel electrophoretic study showed that for peroxidase enzyme, the intervarietal hybrids EP 65 x Pusa Uttam, EP 12 x MDU 1 and EP 104 x APAU Bagmathi and the susceptible variety CO 2 did not show difference in the induction of isoform (PO-1) and all were single and thick. For polyphenol oxidase (PPO), the intervarietal hybrids and the variety CO 2 showed single and strong band induction

The evaluation of genotypes based on biochemical constituents and isozyme studies revealed the superiority of the parents EP 65, APAU Bagmathi and MDU 1and the hybrids EP 65 x Pusa Uttam, EP 12 x MDU 1 and EP 104 x APAU Bagmathi. The identified hybrids with high yield and resistance to shoot and fruit borer could be exploited for commercial cultivation.

ABSTRACT

Astudy was undertaken at Department of Soil Science & Agrl. Chemistry, College of Horticulture, Vellanikkara to standardize different amelioration techniques to prepare enriched coirpith compost. Twelve treatments were tried using different combinations of coirpith, Pleurotus spp., Schizophyllus spp., urea, rock phosphate, cowdung slurry, mushroom waste, goat manure, glyricidia and water hyacinth. The results of the various enrichment techniques revealed that the temperature in the compost in general, increased to a peak value of 47oC on the 22nd day of composting and later decreased to a stabilized value. The observed temperature of 40-50 °C found during the thermophilic stage is essential for the rapid degradation of lignocellulose, as the thermophilic microfungi and actinomycetes involved in this process thrive at that temperature. Though the pH show a hike at the initial stage of composting, it decreased and stabilized at the later stage of composting which is conducive for growth and proliferation of microbes. The treatment coirpith with Pleurotus spp., glyricidia +20% cowdung slurry and goat manure was found to be ideal for compost preparation.

ABSTRACT

Detection of disease tolerance with enhanced defense mechanism is a practical approach in crop variety improvement programme. Toxins being responsible for pathogenicity and virulence of the fungi, regenerating plants from toxin treated calli is an effective and easy approach. The response of tomato calli to the crude toxins of the foliar pathogens viz., Alternaria solani and Septoria lycopersici was studied and the changes in phenol metabolism due to toxin treatment were analysed. Crude toxins of A. solani and S. lycopersici extracted under optimum conditions were serially diluted and tomato calli were treated with different dilutions of the toxins to find out the optimum dilution at which minimum survival percentage was obtained, based on a 1-4 score. Minimum score of 1 was obtained at 1:10 dilution for A. solani and at 1:20 for S. lycopersici.

The calli of resistant (LE-625) and susceptible (CO-3) tomato varieties were treated with desired dilutions of the toxins of A. solani and S. lycopersici. Total phenolics and ortho- dihydroxy (OD) phenolics were estimated at 24 h interval to study the extent of necrosis on resistant and susceptible calli due to toxin treatment. The total and OD-phenol contents of calli of susceptible cultivar (CO-3) progressively decreased with increase in time interval consequent to the treatment of toxins of A. solani and S. lycopersici. In the resistant calli of LE-625, an initial reduction of total phenol and OD-phenol content was observed following the application of the toxins of A. solani and S. lycopersici at 24 hours after treatment. Later with lapse of time, the total phenol content and OD-phenol contents progressively increased reaching a maximum at 120 h after treatment. The results clearly indicated the enhancement of biochemical defense in the resistant calli and a fall in defense mechanism in the susceptible calli due to toxin treatment. Such techniques are useful in the preliminary screening of resistant genotypes in crops like tomato.

Anacardium occidentale is a medium-sized tree, spreading, and evergreen, much branched; grows to a height of 12 m. When grown on lateritic, gravelly, coastal sandy areas, it rarely exceeds 6 m and develops a spreading habit and globose shape with crown diameter to 12 m but, if grown inland on loams; it reaches 15 m tall and is much branched, with a smaller (4-6 m) crown diameter. The root system of a mature A. occidentale, when grown from the seed, consists of a very prominent taproot and a well developed and extensive network of lateral and sinker roots. Leaves simple, alternate, coriaceous, glabrous, obovate, rounded at ends, 10-18 x 8-15 cm, with short petiole, pale green or reddish when young and dark green at mature. Rai and Vatsala (1985) studied the dermal morphology and stomatal ontogeny of Spondias pinnata Kurz., Lannea coromandelica (Houtt.) Merr., Anacardium occidentale L., Holigarna arnottiana Hook. F. and Mangifera indica L. are studied. The cuticle is thick in H. arnottiana, A. occidentale and M. indica, but is thin in S. pinnata and L. coromandelica. Based on the shape of epidermal cells, the five species are categorised into three: cells in both upper and lower epidermis sinuous (A. occidentale, H. arnottiana, S. pinnata); upper sinuous and lower polygonal (M. indica) and upper polygonal and lower sinuous (L. coromandelica). Stomatal ontogeny aperigenous (L. coromandelica), paramesoperigenous (A. occidentale) and anisomesogenous (M. indica, H. arnottiana). S. pinnata is peculiar in that it possesses three different types of stomata, aperigenous, hemiparamesoperigenous and paramesoperigenous; whereas, in all the other four species, only one type of stomatal ontogenesis exists. Except S. pinnata, all the other species possess epidermal appendages. Unicellular trichomes occur in A. occidentale and H. arnottiana, and multicellular glandular trichomes in A. occidentale, M. indica and L. coromandelica. The multicellular hairs are found in A. occidentale in specialized pockets in two rows on either side of the major veins - a unique feature which is reported for the first time.

The term biodiversity is abbreviated from two different terms ‘biological’ and ‘diversity’. Biodiversity is also called biological diversity. The term biological diversity was first coined by wildlife scientist and conservationist Raymond F. Dasmann in 1968. The same term i.e. natural heritage was used in the United State. Geneticists describe it as the diversity of genes and organisms. Biologists define biodiversity as the sum of genes, species and ecosystems of a region.

Biodiversity is determined by the wholesome number of different species in a particular area. It helps to strengthen the stability of an ecosystem and leads to a healthy biosphere. Existence of biodiversity is crucial for the functioning of the ecosystem and is the foundation of life on earth as it generates products and services for the existence of all living organisms on the earth.

1. Introduction

Total population of India is estimated to cross 1.64 billion in 2050. Increasing population, decreasing economic status of Farmer, improper diet and nutrition, fixed farm land, degradation of cultivable land and malnutrition in Indian population is major key problem in 2 to 3 decades. More than 600000 children below 5 year age die due to improper nutrition each year in developing countries (White and Broadley, 1998). All the data provide a scary image about future. Almost 60% of the malnutrition children are iron deficient. Iron deficiency is very common among the girls; 30% each are deficient in zinc and iodine. The incomplete diet and malnutrition can lead to several disorder and degrade the human health (Shuman, 1998).

The Distributed Information Sub Center at Kerala Agricultural University, Thrissur was set up in May 1995 as the 23^rd center of the BTISNet of DBT, Government of India. The area of specilisation of the Centre is Agricultural Biotech- nology. The Centre passed through several phases involving Internet connectivity through the ordinary C band connectivity, then the Ku band connectivity etc. Now the commu- nication facilities of the Centre have been strengthened by procuring an ISDN Internet connection and a connection through ASIANET. The Center caters to the needs of the students (UG, M Sc & Ph D) and faculty of all departments of the Colleges in the main campus as well as of the other nearby academic and research centers of the University. Over the years, DISC has come to occupy a position in the KAU in the area of Infor- mation Technology , information sources and services based on Internet, CD-ROMs etc. The Centre has procured databases in the field of Biotechnology and developed several databases in the field of Agriculture and related fields. The center regularly conducts training programmes in Bioinformatics. The research activities in the field of Bioinformatics have been enhanced. The Centre has now been upgraded to DIC to facilitate intensive research on Bioinformatics in an effective manner. During the year 2004, the Centre has also started offering a course on Bioinformatics for the M.Sc Biotechnology students of the College of Horticulture.

Biotechnology research in KAU spread over various research stations and cam- puses includes Plant Biotechnology, Animal Biotechnology and Fisheries Biotechnol- ogy. Thrust areas for Plant Biotechnology include developing in vitro protocols for mass multiplication of elite types of fruit plants, ornamentals, medicinal plants, spices, plantation crops and forest species. The other thrust areas include checking clonal stability and genetic fidelity of in vitro derived plants using molecular markers, DNA finger printing and characterisation of germplasm with molecular markers, imparting biotic and abiotic stress tolerance in otherwise accepted crop varieties, genetic trans- formation for imparting/ improving expression of desired traits, in vitro germplasm conservation, enhancement of secondary metabolites production etc.

The crops identified for biotechnology research at KAU include spice crops like black pepper, ginger, turmeric, vanilla, cardamom; plantation crops like cashew, coco- nut and medicinal & aromatic plants such as Kaempferia, Trichopus, Gymnema, Holostemma and Coscinium. For improvement of such crops through molecular breed- ing, it is highly essential to identify/design specific probes/primers, micro-satellites, etc. By combining the research results generated at the Biotechnology Centre and informa- tion retrieved from the Bioinformatics Centre, suitable probes, primers are being de- signed for characterization and improvement of these unique crops through molecular breeding.

Fruits with their delightful colours, flavours and tastes are nature’s wonderful gift to mankind. Apart from being the most nutritious food in the form of easily digestible simple sugars, vitamins, minerals and many phyto-nutrients (Plant derived micronutrients), they provide unique health benefits due to their antioxidant properties and valuable fibre. Antioxidants help protect the human body from oxidant stress, diseases, and cancers, and also boost the immunity of the body thus developing the capacity to fight against various types of diseases. The insoluble dietary fiber in fruits is known to provide protection from conditions like chronic constipation, hemorrhoids, colon cancer, irritable bowel syndrome, and rectal fissures etc. Due to their unique nutrition-profile the importance of fruits in our diet cannot be over emphasised.

Insects are a major limiting factor in fruit production. The infestation of insects pests on fruit trees in orchards adversely affects the quality and yield of fruits. The size, shape, taste, attractiveness and palatability of fruit deteroriates, due to which the market price is reduced and the growers incur losses. Fruit growers resort to use of chemical pesticides under such conditions. However many reports suggest that although the global production and use of pesticides in agriculture is increasing every year, but the damage due to insects has not decreased rather it has also increased, indicating that the use of pesticides is not the panacea remedy for all insect related problems. Many scientists are of the opinion that over-reliance on the use of synthetic pesticides in crop protection programs around the world has resulted in disturbances to the environment, pest resurgence, pest resistance to pesticides, and lethal and sub-lethal effects on non target organisms especially beneficial flora and fauna, including humans. Farmers spend thousands of rupees on pesticides. The residues of pesticides in food and fruits is yet another cause of concern. Fruits which are considered to be wonderful for health are often laden with unacceptable levels of pesticide residues nulling their beneficial properties. Pesticides have been linked to many diseases especially cancer. All these issues related with use of pesticides has triggered rethinking on the methods of control.The recent examples of the results of indiscriminate pesticides use , consequent crop failures and suicide by farmers now calls for introspection in this matter.

Biological control or biocontrol is a method of controlling pests such as insects, mites, weeds and plant diseases using other organisms. It relies on predation, parasitism, herbivory, or other natural mechanisms, but typically also involves an active human management role. It can be an important component of integrated pest management (IPM) programs.

1.0 INTRODUCTION

“Biological control is the reduction of the amount of inoculum or disease producing activity of a pathogen  or parasite  in its active or dormant state, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonist or by mass introduction of one or more antagonists ” (Baker & Cook 1974). The biological world is a vast interacting network of living organisms in a state of dynamic equilibrium, reflecting changes in their physical environment and their relations to each other. Organisms exhibit many type of interactions in the process of achieving biological balance.  An organism may exert no effect on another or it may stimulate or inhibit the growth or development of the associate, it may stimulate or inhibit the formation of resting spores of the associate or it may enforce dormancy or cause lysis of the associate (Cooke & Baker, 1983).

1.0 INTRODUCTION

Infestation by insect pests is a major factor responsible for the low productivity of spice crops in India. Spice crops such as black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton), ginger (Zingiber officinale Roscoe) and turmeric (Curcuma longa L.) are infested by many species of insect pests, some of which cause severe crop losses. Adoption of integrated pest management (IPM) schedules in which biological control forms a major component is more relevant in these crops since excessive use of insecticides could lead to pesticide residues in the produce and other ecological hazards. Spices are high-value and export-oriented in nature and pesticide residues are the most important non-tariff barriers on trade in these produces. There has been a renewed interest during the past decade in developing environment–friendly crop management schedules in sustainable agriculture including spice crops, reflecting the increasing concern over pesticide misuse.

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

I. INTRODUCTION
The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:
1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.
The job of postharvest technologist is to understand life in fruits, vegetables and flowers and prolong their life as much as possible. The post harvest life of produce depends on the rate at which they use up their stored food reserves and their rate of water loss. The sugar and starches (carbohydrate derivatives) are stored in various parts of plants through a process known as photosynthesis occurring in growing plants and are used later to provide energy for further growth, development and to prolong post-harvest life. The process of photosynthesis in growing plants and respiration in harvested produce can be seen in Fig. 2.1. The released energy through process known as respiration occurs in plants before and after harvest. Thus, principal normal physiological processes leading to aging are respiration and transpiration.

Ethnopharmacology and Traditional medines use natural resources as medicines. They have developed the knowledge of this through the generations of traditions and experiences. Therefore the protection of this knowledge and protection of natural resources both remain critical. Bioprospecting involves sustainable, ethical and legal processes to access natural resources and use them judicially so that the business needs do not pose any threat to nature and to people.

One important consideration is assuring the preservation of traditional knowledge, particularly oral knowledge, within communities, for the potential benefit of others. Developing mechanisms to document traditional knowledge, such as national inventories, can be beneficial in identifying cases where conservation or cultivation is needed, based on an understanding of existing natural resources used for medicinal purposes. Decisions about access and ownership of information can also be made (including those relating to intellectual property rights, where appropriate) once methods have been found to collect and preserve traditional knowledge. On the other hand, the livelihood of traditional healers may depend on maintaining the secrecy of their healing methods - one argument for consulting with traditional healers as part of the process of planning and executing the collection, storage and testing of traditional medicines.

Extending some form of intellectual property protection to traditional knowledge, of which traditional medical knowledge is a significant part, is being actively debated in WIPO and other international forums. Existing intellectual property regimes can offer protection for purified compounds (produced by scientists and companies) isolated from a natural medicine. But traditional medicines, whose method of action may not be well understood, and which often consist of mixtures of different active substances, may not meet criteria for patentability. The principal impetus for international debate is a desire to compensate the holders of traditional knowledge appropriately for the use of their knowledge by others, or to prevent so-called “biopiracy” where knowledge or genetic materials are used without the consent of the holder. China and Kenya are examples of countries that have modified their laws in an effort to accommodate traditional medicine. It is not intended that the Commission should enter into this debate, except in so far as aspects of it may be relevant to its terms of reference, in particular stimulating innovation in traditional medicine with the purpose of expanding access to affordable medicines.

1. INTRODUCTION

Chemical pesticides are designed to control or eliminate pests such as insects, rodents, weeds, bacteria, and fungus. Although pesticides have played a significant role in increasing food production and controlling various diseases in the agriculture system but indiscriminate use of pesticides has resulted in contamination of soil, water bodies’ etc. raising deep concern about food safety and human health. Excessive use of these harmful chemicals affects kidneys, developing fetus, and liver immuno-suppression and increased incidence of breast cancer. Some of these pesticides are also reported as mutagenic. Other health related problems such as impaired memory and concentration, severe depression, headache, etc have also been reported.

Through field survey (Kadian et al., 2010) it has been found that chlorpyrifos is a widely used pesticide in household application as well as in horticulture crop in National capital region Delhi. This tempted us to understand the nature of chlorpyrifos (CPF) and its metabolites and assess the suitability of available techniques for detoxification of contaminated soil.

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

. Introduction

A primary issue for modern horticulture is facing two contradictory objectives, such as the need to produce food for the increasing world population and to minimize damage to the environment, which can in turn negatively impact horticulture. Fertilizer and chemical use and manufacture are hazardous to the environment and humans. As a result, new and effective environmentally friendly compounds are required. Plant biostimulants, which are harmless for humans and the environment, are one answer. They are particularly useful for lowering pesticides in agriculture. Plant biostimulants have become popular in farming and cultivation in recent years.

ABSTRACT

The use of engineered nano-materials has been increased as a result of their positive impact on many sectors of the economy including agriculture. Silver nanoparticles (AgNPs) are now used to enhance seed germination, plant growth and as anti-microbial agents to control plant diseases. This study investigated the synthesis of silver nanoparticles using the aqueous solution of three medicinally important plants (such as Spondius mombin L., Stachytarpheta jamaicensis (L.) Vahl and Syzygium samarangense (Blume) Merr & Perry leaf extract in room temperature (350C). AgNPs present in the leaf confirmed by colour change, UV-VIS spectrum analysis, average size of AgNPs confirmed by PAS. FTIR measurement were carried out to identify the other possible biomolecule groups such as alkenes, esters, phenols, alcohols and aromatic. Leaf extract AgNPs were shown significantly higher antimicrobial activities against four species of bacteria. This findings suggest that the seed germination percentage, relative seed germination rate, relative shoot and root growth and germination index of the tested plant depends upon concentrate gradient of AgNPs.

ABSTRACT

Recognizing the need to apply plant tissue culture tools for generating planting materials of important spice crops especially vanilla, the Spices Board started Biotech Production Unit at its head quarters building at Kochi with an annual production potential of 8 lakhs plantlets. Complete tissue culture protocols were developed and scaled up for large scale production of vanilla and other spices viz. large cardamom, small cardamom and tree spices. The laboratory is equipped with all modern facilities and is established in an area of about 2000 sft. Department of Biotechnology, Govt. of India also participated in the venture by sanctioning a project entitled “Regional centre for large scale production of tissue culture plantlets of vanilla and other spices” with a financial out lay of Rs 95 lakhs equally shared by DBT, New Delhi and Spices Board, Cochin.

At present there is a stock of over 3.00 lakh vanilla cultures in the lab and already 1.25 lakh plant lets were sent for hardening. The Spices Board, in coll aboration with “Kudumbashree”, a poverty eradication mission of Govt. of Kerala, extend short term training in tissue culture techniques to undergraduates from ‘Below Poverty Line’ (BPL) families and engage them as long term trainees in the Biotech Production Unit. The Board also coordinates and extends support to Kudumbashree members for, establishing and maintaining hardening facilities for TC plantlets thus providing means for empowering women from BPL families.

Role of Biotechnological Approaches

In our fore-going chapters, we have studied variety of plant products such as alkaloids, glycosides, volatile or essential oils, gums and mucilage and oleoresins with varied role to play to the plants as well as to human.Knowledge of plants and knowledge of healing have been closely linked. It is well established that industries have many direct and indirect effects on human population. Increased stress is most evident at the same time health awareness goes parallel in the recent times. Most of the natural plant products are compounds biosynthetically derived from primary metabolites such as amino acids, carbohydrate metabolic intermediates and fatty acids. These secondary metabolites are the major sources of pharmaceuticals, food additives, fragrance and pesticides.

Application of biotechnology know how helps to synthesize the novel molecules and also could be beneficial to enhance the production of secondary metabolites. Classical definition of biotechnology states that it is “The use of living organisms or their products to enhance our lives and our environment.” The word Pharming is commonly use to describe genetically engineered plants or livestock to produce medically useful products –predominantly protein drugs. Plants or animals are genetically engineered to contain genes capable of producing a biologic or drug compound. Therapeutic proteins are designed to treat illness includes serum proteins, cytokines, growth regulators, anti coagulants, antbiotics, lysosomal enzymes and plant secondary metabolites.