eChapters

Introduction
According to the U.S. Food and Drug Administration (FDA), Good Laboratory Practices (GLP) is a quality system that ensures the nonclinical safety studies are appropriately designed, conducted, and documented to generate reliable data. The laboratory techniques are defined as the processes and practices that are recommended for equipment use in the laboratory, while laboratory methods are based on established scientific principles involving biology, chemistry, and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol in blood to analysing DNA to growing microscopic organisms that may be causing an infection. One of the most significant parts of laboratory techniques and laboratory methods is the proper validation of the equipments (old or new equipments) as well as all the testing procedures/ methods (established or newly developed methods). Analytical method validation is a critical process to ensure the quality and safety of products. The objective of the validation of an analytical method is to demonstrate that it is suitable for its intended purpose; the results are authentic and universally acceptable. An analytical method is suitably well considered when the results generated through different method are consistent, reproducible, and thus, reliable.
The validation is required when
• A new method has been developed
• Revision of established method
• Use of established methods in different laboratories and different analysts, etc.
• Comparison of methods required
• When quality control indicates method changes

Like all snake poisons, Lachesis decomposes the blood rendering it more fluid; hence a haemorrhagic tendency is marked. Purpura, septic states, diphtheria, and other low forms of disease when the system is thoroughly poisoned and the prostration is marked. Delirium tremens with mucfrtrembling and confusion. Very important medicine during menopause and for patients of a melancholic disposition. Diphtheria carriers. Sensation of tension in various parts.

Lachesis treats the worst of throat problems, hot flushes in women during menopause, and diptheric paralysis. It is indicated in high blood pressure with constriction and in heart disease when the face is purple and bloated. Also religious insanity.

As a red inflammation leads us to use Belladonna, so Lachesis is to be thought of when the colour is purple. The face is purple; the eyes are engorged. An inflamed spot is purple and so are the inflamed glands. Ulceration bleeds, blood coagulates and the surface looks purple. There is extreme sensitivity to touch. The throat is purple and not red because the blood under the skin has coagulated. There is nothing like Lachesis if these indications are present.

Introduction

Increasing concern about climate change and global warming has created immense awareness among society about green house gas and its reduction. Irrespective of the geographical location, population density and economic development of any region, there is a huge scope for addressing the green house gas emission. Cities have a key role to play in the global agenda for addressing the challenge of climate change. Today, approximately half of the world’s population lives in cities; by 2050, that proportion will probably have increased to two-thirds. India has the highest rate of change of the urban population and will remain above 2% annually for the next three decades. At this rate, an estimated 854 million people will live in Indian cities by 2050.

Introduction

Landscape gardening is a very fascinating and interesting subject. It is a money spinner business and people who are involved in designing and preparing landscape gardens are earning handsome money. It is an aesthetic branch of horticulture which deals with application of garden forms, different styles, methods and materials with a view to improve the landscape. Frescos of flowers of old times reveal the association of human with flowering plants which they used in making gardens and planting on road side. In India, the landscape gardening is a developing subject and as such offers much scope for improvement. A beautiful landscape is a vital part of your home and community. A well-planned landscape can save your money by reducing energy consumption.

Grasshoppers: Mecopoda elongata :Tettigonidae; Oxya velox: Acrididae

These pests occur both in nurseries and plantations. Both adults and nymphs feed on leaves by scrapping and eating outer tissues and margins. The emerging leaves are nibbled and the spot holes appear when the leaf unfurls. In severe conditions the entire leaf is eaten leaving behind only the midrib.

Dioscorea alata is commonly called greater yam or larger yam or water yam.  This is the most widely distributed species of yam. This species originated in South East Asia, probably in Burma. From there, it first spread to India, Malaysia, Indonesia and the eastern portion of South East Asia.  Later it served as food for voyagers and thereby spread to other parts of the tropics.  Immigrants from India and Malaysia introduced it to Madagascar, from where it was partially introduced into East Africa and Portuguese and Spanish voyager traders of the 16th century probably introduced it into West Africa and later to West Indies.

11.1. Origin and distribution

• Indigenous to Mediterranean region, growing wild in higher hills of Southern France, Italy and Bulgaria.

11.2. Economic importance

• True lavender (Lavendula angustifolia Mill sub. sp augustifolia)

• Spike lavender (Lavendula latifolia Mill sub. sp spica)

• True lavender - used in perfumery industry.

• Lavandin - hybrid between true and spike lavender

• Lavender oil - delightfully clean, refreshing and sweet odour.

• Main constituent - ester, linalyl acetate 30 to 60%

• Another compound - ethyl-n-amyl ketone.

• Free linalool - major component

Scientific name: Lavandula angustifolia Mill.
Family: Lamiaceae
Lavender is a small genus of perennial aromatic herbs, semi-shrubs, or shrubs. It comprises approximately 28 species, with three species being extensively utilized for extracting essential oils:
True Lavender (Lavandula angustifolia): One of the most important essential oils used in the perfumery industry.
Spike Lavender (Lavandula latifolia): Yields inferior oil, less fragrant than true lavender and rosemary.
Lavandin (Lavandula hybrida): is a hybrid of true lavender and spike lavender, possessing characteristics of both parents. The essential oil extracted from Lavandin combines the sweet, floral fragrance of true lavender with the camphoraceous harshness of spike lavender. Although Lavandin oil has a unique fragrance profile, it is considered inferior to true lavender oil in terms of quality and finesse.
Origin and distribution
Lavender originates from the Mediterranean basin, typically thriving in rocky, calcareous areas. Its distribution spans North Africa, the Mediterranean, Europe, and Western India. Lavender has been cultivated since ancient times, with the Greeks and Romans being notable cultivators. The name "lavender" is derived from the Latin verb "lavare," meaning "to wash" or "to bathe."

Lawn

Lawn is a natural green carpet, and it is important feature of a landscape and a garden without a lawn is not considerd complete. Lawn is an area of land closed mowed grasses it is primarily developed for aesthetic and recreational purpose. A lawn is an integral part of a garden and landscape besides aesthetic and recreational purpose and several other purposes. Lawn provides a place for taking rest after tiring of the day, holding parties, social functions, passive and active recreation. Distinguishing characteristic of turf grasses is the ability to with stand close mowing and still providing a functional dense healthy ground cover. Lawn is also called as heart of garden. Lawn can be called as turf pitch a field, sod, yard, are green depending upon the plantation usage continent. Lawn is a ground cover of perennial grass, ditch, persists enclose mowing and require proper management practice.

J.B. Olcoth designed first experiment in lawn grass at Monchester in USA in the 19th century. Festuca and Agrostis species were used to make turf (Lawn) during 1880 in U.K. In the early 19th century lawn become wild spread out side of parks golf course. Now a day in many metro cities of India lawn has become a cultural of the passion of many homes.

Lawn

Lawn is a natural green carpet, and it is important feature of a landscape and a garden without a lawn is not considered complete. Lawn is an area of land closed mowed grasses it is primarily developed for aesthetic and recreational purpose. A lawn is an integral part of a garden and landscape besides aesthetic and recreational purpose and several other purposes. Lawn provides a place for taking rest after tiring of the day, holding parties, social functions, passive and active recreation. Distinguishing characteristic of turf grasses is the ability to with stand close mowing and still providing a functional dense healthy ground cover. Lawn is also called as heart of garden. J.B. Olcoth designed first experiment in lawn grass at Monchester in USA in the 19th century. Festuca and Agrostis species were used to make turf (Lawn) during 1880 in U.K. In the early 19th century lawn become wild spread out side of parks golf course. Now a day in many metro cities of India lawn has become a cultural of the passion of many homes.

Objectives

1. To know the layout methods.
2. To know the planting techniques of various spices and condiments.

Layout: Systematic arrangements of plants

Some spices and condiments just like clove, cardamom is perennial in nature. Being perennial in nature any mistake committed during initial year, on the part of planning, is hard to rectify. Hence, all the factors having bearing on growth, production and overall performance of the plant should be given top priority.

Systems of planting

1. Square system: In this system row to row distance and plant to plant distance is kept similar.

1. INTRODUCTION

Leaf and soil analysis are the tools to estimate the fertilizer needs of fruit crops. Leaves are selected for analysis because:

• Leaf is the principal site of plant metabolism.
• The changes in nutrient supply are reflected in the leaf composition.
• The changes are more pronounced at certain stages of development.
• The concentration of nutrients in the leaf at specific growth stages are related to performance of the plant.

8.1 Key legal statutes and regulatory foundations
Various policy measures were also undertaken by the Government of India in due course of time. The Government of India enacted four science policies after gaining independence, i.e. Scientific Policy Resolution (1958), Technology Policy Statement (1983), Science and Technology Policy (2003) and Science Technology and Innovation Policy (2013). The 1983 Technology Policy Statement possessed a simple goal of creating indigenous technology and boosting efficient absorption and adaptation of imported technologies suited to the nation’s priorities and. The Technology Policy Statement of 1983 was followed up through the establishment of the Technology Information Forecasting and Assessment Council (TIFAC), an autonomous organization entirely owned by the Government of India under the Department of Science and Technology during 1988. TIFAC also offers some funding and supports development and commercialization of technologies through the home-grown technology scheme, among its other operations. The Technology Development Board Act (1995) was also a significant policy move of the government in the same direction. Also in recent times, the Government of India also unveiled its own National IPR Policy 2016 with the motto cum slogan “Creative India, Innovative India”. In addition, the policy is aligned to a number of other schemes of the Indian Government like “Make in India”, “Atal Innovation Mission”, “Start-Up India” and “Stand-Up India”, all aimed at encouraging creativity, innovation and entrepreneurship in the nation.

Leguminous vegetables include peas, French bean, Lima bean, broad bean, cowpea, hyacinth bean, winged bean etc. They belong to the family Fabaceae (Syn. Leguminosae). These are cultivated either as sole crop is sometimes as intercrop. In some areas they are used in backyard cultivation also. Most of them are short duration crops and are self pollinated.

8.1. Peas (Pisum sativum L.)

The pea (Pisum sativum L.) is a very common nutritioius vegetable grown in cool season throughout the world. It belongs to the family Fabaceae (Syn. Leguminaceae) and genus Pisum. A pea is most commonly the small spherical seed or the seedpod of the pod fruit Pisum sativum L. The pea cultivars are grouped into several groups based on shape of seed, plant height, maturity and use of pods. This crop is cultivated for its tender and immature pods for use as vegetable and mature dry pods for use as a pulse. In both the cases the seeds are separated and used as vegetable or pulse. The central Asia is regarded as the place of origin for all legumes including pea. Based on the genetic diversity, Central Asia, the Near East, Abyssinia and the Mediterranean is considered to be its centre of Origin. Cultivated garden pea is not seen in wild state and it might have been originated from wild field pea or other related species. In India it widely cultivated states of Uttar Pradesh, Bihar, Haryana, Punjab, Himachal Pradesh, Orissa and Karnataka. Followed by Madhya Pradesh, Himachal Pradesh, Assam and Jharkhand.

Cultivars/Varietie: Peas varieties are grouped based on maturity (early, mid season and late) plant height (Bush or dwarf, medium tall and tall). The major cultivars under cultivation in different parts of the country are Arkel, Bonneville, Harbhajan, FC-1, Arka Ajit, UN 53-6 (whole edible type), Jawahar Matar 1, Jawahar Matar 2, Jawahar Matar 3, Jawahar Matar 4, Jawahar Matar 5, Jawahar Peas 83, JP 4 (JM 6), JP 19, Lincoin, Mattar Ageta 6, Pantnagar Matar 2, Pant Uphar (IP 3), P 88, PRS 4,Ooty-1 and VL 3.

2.1. Origin and distribution

• Native to India and Sri Lanka.

• Widely cultivated in tropics and subtropics.

2.2. Economic importance

• Cochin grass or Malabar grass or East Indian or True Lemon grass; perennial and multi cut aromatic grass.

• Lemon like odour due to presence of 70-90% citral, a cyclic monoterpene.

• Other compounds are geraniol, citronellal etc.

Shoot borer: Chilo infuscatellus : Pyralidae

This pyralid is more commonly known as a pest of sugarcane. Eggs are laid in batches on lower side of leaves.  The emerging larvae move down and cut a hole at the ground level and enters the stem.  Due to feeding of larva, the central shoot begins to wilt and dry causing dead heart. The yield of the crop is affected.New tillers may develop but will not have the physiological maturity and oil content at the time of harvest.

Introduction
Lemongrass grows widely in many tropical and subtropical parts of Asia, Africa and America. East Indian or true lemongrass (C. flexuosus) is indigenous to India and large variability occurs in the Western Ghats comprising Kerala, Karnataka and T.N., besides Sikkim and A.P. Its cultivation and distillation of oil started first in Kerala and now grown in Assam, Maharashtra and U.P. The strong lemon like odour of the oil of the grass is responsible for its nomenclature. Predominantly, there are two types of lemongrass under cultivation, viz., the East Indian or Cochin or Malabar grass (Cymbopogon flexuosus) with superior oil quality (75-85% citral) and preferred by the industry.
The other West Indian or American lemongrass (Cymbopogon citratus (D.C.) Stapf. is mainly cultivated in West Indian islands, Guatemala, Brazil, Mexico and Madagaskar; and comora islands, Thailand, Bangladesh and China. It has lower citral content and lower solubility in 70% alcohol. It is indigenous to India and spread over Western Ghats, comprising Kerala, Karnataka, TN, Sikkim, Arunachal Pradesh, Assam, Maharashtra, parts of Uttar Pradesh, Punjab and Haryana.
Besides, a separate culture called Jammu lemongrass (Cymbopogon pendulus) is another indigenous species found wildly growing in West Bengal, Sikkim, Assam and M.P. It also has high citral content, but cultivated to a limited extent in parts of Jammu in northern India.

Wilt appears in the fi eld as patches is both seedling stage and developed plants. Symptoms include drooping and wilting of uppermost leaves. Plants become completely yellow and die. Plants are affected during the pod fi lling stages, seeds are often shrivelled. There will be rotting at the basal portion of the plant. Discoloration of vascular bundle are noticed when the stem is spitted
open.

The North East region represents eight states namely Assam, Arunachal Pradesh, Meghalaya, Manipur, Mizoram, Tripura, Nagaland and Sikkim consisting 7.79% of the Indian national geographical area. The altitude difference (50m-7000m) coupled with varied physio- geographic and agroclimatic conditions; varying intensity of rainfall (800-12000 mm) and topography harbour various vegetation types of tropical, temperate and alpines zones. In India, there are about 800 plant species used as food plants chiefly  by the tribal population (Singh and Arora, 1978). Out of these about 300 species are prevalent in the northeastern region (Khrasati, 1973, Joseph and Murti, 1981, Singh et al. 1981).

Tribals of various ethnic groups like Khasi, Garo, Monpas, Karbis, Jaintia, Naga, Kuki, Manipuri, Mizo, Chakma, Dafla, Adi, Mishing, Apatani and others are predominant in the various habitats of hilly regions also used to practice “Jhumming” or shifting cultivation. Most of wild fruits are used  in day to day diets of tribal people of the region and are often sold in the local markets.

Lesser yam, Lesser Asiaticyam (Dioscorea esculenta) is among the most ancient species of the genus and its centre of origin is stated by various authorities as India, Vietnam or Papua New Guinea and the Philippines. It is domesticated as a staple food in Southern China from 2nd and 3rd centuries. Today, it is widely distributed throughout the tropics, but is little used except in South East Asia, where it is grown to such an extent that it ranks third in production and utilization of yams after D. rotundata,D. cayenensis and D. alata. Wild types of D. esculenta also exist in Malaysia, New Guinea and Philippines.  The wild types have more leaves, large roots, longer stolons, more fibrous tuber flesh and more thorny roots and shoots.

Classification
The wild types of D. esculenta (Family: Dioscoreaceae) exist in Malaysia, New guinea and Philippines. Wild types have more vigorous foliage, larger leaves, longer stolons, more fibrous tuber flesh and more thorny roots and shoots.  On the basis of thorniness, the cultivated types are distinguished into two botanical varieties.