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For the first time, the Quality Planting Material (QPM) production from aerial stem node cutting and its comprehensive growth parameters are being reported in Valeriana jatamansi (Jones) (family: Valerianaceae), a highly endangered medicinal plant and a natural habitat in North Eastern Himalayan Region in the high altitude ranging from 1000 to 8000m Above Sea Level (ASL). The present communication identified a novel avenue exploring the unused plant part, aerial node cutting for producing QPM in a continuous and exponential rate without scarifying the rhizome and root, enriched with active ingredients like Valerenic acid and Valepotriates. This method also assures 100% plant emergence and more than 91±5.56% plant survival in the different field conditions without requiring hardening process as an additional merit. This first-time report for QPM production of an endangered medicinal plant, Valeriana jatamansi through aerial node cutting without following the hormone supplemented MS based tissue culture, a high cost process required sophisticated instruments and aseptic environment. This present process will have potentiality to explore commercially for mass clonal-propagation even at farmer level.

ABSTRACT

A rapid micropropagation technique have been established for Solanum trilobatum L., a medicinal plant from internodal explant. Callus formation was observed from both cut ends of explants cultured on MS medium supplemented with 2,4-D (3.0 mg/l). Direct shoot and multiple shoot formation were noticed from explants (proximal cut end) on BAP (2.0 mg/l) alone and BAP (2.0 mg/l) + NAA (0.5 mg/l). Root formation was directly produced from the basal cut end of explants cultured on IBA (1.0 mg/l). In vitro plantlet regeneration has been achieved on BAP (2.0 mg/l) + IBA (1.0 mg/l). Regenerated plants were transferred to sterile vermiculite and soil mixture (3:1). In vitro propagated plants were grew successfully under the field conditions. In vitro propagation can be a useful tool for the conservation of this medicinal species.

ABSTRACT

The Kolanthamamalai hill an offshoot of Kalakadu Mundanthurai Tiger Reserve of Southern Western Ghats is one of the important forest reserves of Tirunelveli District. The physiography of this range differs much from the adjacent portions of Southern Western Ghats of Tamil Nadu state due to its discontinuity, varying altitude, ridges and their height and breadth. The altitude of the hilly rugged terrain ranges from 150 to 300m, with the highest peak being Kolanthamamalai. Field visits were made in the study area to survey and to gather information on medicinal flora. The vegetation of Kolanthamamalai hill is a mixed dry deciduous type characteristic of the high and low altitude of the mountains respectively. Lowland vegetation girdles the base of the mountain on the eastern and southern side of the hills. Along the foot hills vegetation is an open scrub with occasional trees. The low-lying areas and the seasonal watercourses are densely vegetated. The natural barriers, habitat diversity, climate and moderate rainfall have resulted in the development of unique plant diversity.

Objectives

1. To identify the morphological character of different spices and condiments.
2. To know the best variety of particular crop in that region.
3. To know the problems which are the main drawback of that region in the cultivation of spices and condiments.
4. To know the feedback of farmers.

General information of Institute

1. Name of Institute:
2. Address of the Institute:
3. Establishment year of the institute:
4. Director of the Institute:
5. Total area of the Institute:
6. Climatic condition of Institute:

Okra (Abelmoschus esculentus Moench), known in many English-speaking countries as lady’s finger or gumbo, belongs to family Malvaceae. The geographical origin of okra is disputed and supposed to be South Asian, Ethiopian and West African origins (Anonymous, 20013a). Its proposed parents are reported to be in South Asian; therefore, it is believed that perhaps it is of South Asian origin. However, because of greater diversity in okra in West African region, it is considered to be originated in West Africa. Masters (1875) proposed that it originated in India.
Okra is cultivated in tropical, subtropical and warm temperate regions, including the Middle East, Africa, Brazil, Turkey and southern states of USA (IBPGR, 1990; Jideani and Adetula, 1993; Acquistucci and Francisci, 2002). In tropics it is cultivated during summer and in the warmer parts of the temperate regions. It is an annual or perennial plant, growing up to 2 m height. The leaves are 10–20 cm long and broad, palmately lobed with 5-7 lobes. The flowers are 4–8 cm in diameter, with five petals which are generally yellow, but often white with a red or purple spot at the base of each petal. The fruit is a capsule which may attain length up to 18 cm containing numerous round seeds. Okra is an allopolyploid of uncertain parentage (proposed parents include Abelmoschus ficulneus, A. tuberculatus and a reported “diploid” form of okra).

Chemicals which are used specifically for the control of mite pests are known as acaricides, though there are many insecticides, which show acaricidal properties as well. In the last few decades, there has been tremendous progress in the development of broad-spectrum acaricides i.e. the compounds that will knock down different species of mites at the same time. Prior to the commercial introduction of broad-spectrum insecticides like DDT and other organochlorine insecticides, mites did not commercially cause wide-spread damage under natural or semi-natural environments which were little influenced by man. This might be due to the natural processes of prey-predator relationship in undisturbed situations. Contrastingly in situations where broad-spectrum pesticides are commonly used, the natural enemies are more adversely affected than the mites, and an outbreak of the latter occurs. Besides becoming generally intensified with the introduction of modern insecticides, the mite problem in some cases has subsequently become even more serious due to development of resistance against these materials.

Many spider mite species, which were rarely found in pest form in some situations, appeared commonly as major pests with the persistent use of pesticides. In Indian literature, spider mites were never reported in pest form prior to 1940 but with the intensive use of pesticides, every year new mite problems are appearing and becoming troublesome to control.

This chapter provides a readily available means of reference for helping in life threatening emergencies, accidents and for providing first aid, where any delay caused by the lack of medication or non-availability of the doctor can have serious consequences either in terms of life or in terms of resulting serious sickness.

As soon as possible competent medical help should be availed of in emergencies and accidents. In the meanwhile the homoeopathic medicines suggested hereafter would provide relief, would halt deterioration in most cases and may save life in many instances.

Emergencies can be life threatening. One of these can be severe haemorrhage, where copious bleeding can lead to death. Obstruction of breathing for different reasons is a matter for concern. Serious accidents are life threatening because there may be unmanageable bleeding, shock and pain.

Below, a list of few medicines and other items required for first aid in emergencies has been given. This can be helpful in homes, in factories and offices.

Acetylcholinesterase activity in brain is measured by the method described by Ellman’s et al. (1961).

Reagents

1. Phosphate buffer (0.1M) (pH 8.0) Solution A: 5.22 g of K2 HPO4 and 4.68 g of NaH2 PO4 are dissolved in 150 ml of distilled water. Solution B: 6.2 g of NaOH is dissolved in 150 ml of distilled water. Solution B is added to solution A to get desire pH 8.0 and then finally the volume is made up to 300 ml with distilled water.

2. DTNB reagent 39.6 mg of DTNB with 15 mg of NaHCO3 is dissolved in 10 ml of 0.1 M phosphate buffer.

3. Acetylthiocholine (ATC) 21.67 mg of Acetylthiocholine is dissolved in 1 ml of distilled water.

Procedure

The brain tissue is weighed and 10% homogenate is made in 0.1M Phosphate buffer (pH 8.0). Take 0.4 ml aliquot of homogenate in a cuvette containing 2.6 ml phosphate buffer (0.1M) and 100 µl DTNB. The contents of the cuvette are mixed thoroughly by bubbling air and absorbance is measured at 412 nm in a spectrophotometer. When absorbance reached a stable value, it is recorded as the basal reading .To this 20 µl of acetylthiocholine is added and change in absorbance is recorded for a period of 10 min at intervals of 2 min. Change in the absorbance per minute determined.

Pimples are very common in young people during their teen years. This condition, which is also known as acne, is apparently due to certain hormone changes in the body. It is particularly prevalent during early adolescence, from 12 to 16 years of age, and is more likely to occur in those who have an oily skin. In the early stages a blackhead or comedone blocks the outlet of an oil gland within the skin. This is not a serious problem, for it leaves no permanent effect.

If the sebaceous duct ruptures and the oily material ooze out between the layers of the skin instead of coming to the surface, there may be a cyst or swelling known as a sebaceous cyst. These are somewhat disfiguring, but not harmless unless they become infected and form small abscesses. When these heal they may leave ugly scars. Severe acne may occur on the shoulders, the back and chest, as well as the face and neck.

Effects of diet: Acne is not caused by diet, but certain foods definitely aggravate this condition, especially in some patients. Rich foods, such as chocolate, nuts, cola drinks, ice-cream, fatty meats, and excessive amount of sugar, all seem to aggravate this condition. Certain drugs, such as those containing bromides and iodides, may also play a part in some people.

Some girls develop one to two pimples just before menstruation. These only last a day or two and are of no consequence. But when acne continues over months or years, it is very distressing, especially to a young woman, and something should definitely be done to clear up the condition. Nervous tension due to family trouble may also play a part in continuing this difficult problem. Even in its mildest form, acne usually persists for a year or more until the hormone system of the body has become fully adjusted.

The word Aconite is derieved from ACONIS, a city of Bithynia (in Asia Minor). Also ACON means dart because the darts were poisoned with Aconite. Also, from Greek word ACONITUM means without soil, as this plant grows on a stony ground.

History and Authority: Master Hahnemann introduced it in homoeopathy in 1805.

A state of fear, anxiety; anguish of mind and body. Physical and mental restlessness, fright, is the most characteristic manifestation of Aconite. Acute sudden and violent invasion, with fever, call for it.

There is no medicine like Aconite Nap when a complaint is caused by exposure to dry cold weatlier or by fright or both. Aconite should be thought of as the first medicine in inflammation, inflammatory fevers, when an attack is acute, sudden and violent, when there is physical and mental restlessness and if the problem is recent and when all this is on account of exposure to dry cold weather. The parts of the body affected can be larynx, bronchi, pleura joints and even heart.

The success of a TTO relies on a systematic approach encircling factors like assessment, valuation, marketing, negotiation, and post-transfer management (fi gure 8). This chapter covers the activities that are essential for the effective functioning of a TTO. Other activies Technology Assessement /Valuation Financial Managemnt Major Activities of technology transfer office Commercialization IP Management Fostering Innovation
Fig 8: Various activities of TTOs.
3.1 Technology assessment - A pillar of innovation commercialization
The process of transitioning an innovative technology to commercial reality requires a stringent and formal process known as technology assessment. This early stage is crucial as it determines the viability of an invention by analysing practicability of the invention as well as its uniqueness and the likelihood of it being adopted in the target markets. In the absence of this preliminary examination, the chance of technology’s potential being mismatched to the market can arise.
a) Scientific merit and technical feasibility
A key benchmark in assessing technologies involves the identification of
scientific validity and technical performance. An innovation’s viability depends on the fact that it complies with good scientific practice or the innovative application of a new paradigm that has empirically confirmable validity. It is important to determine whether the invention’s underlying mechanism can be reliably proven through established scientific practice. A working prototype allows for empirical testing in different conditions and serves as proof of practical application. In addition to that, scalability has to be tested critically. It often happens that innovations perform well in a controlled laboratory setting but lose efficiency or cost-effectiveness in the case of mass production. The viability of the implementation on a grand scale becomes a core concern in this phase of the evaluation.

Litchi (Litchi chinensis Sonn.), a subtropical perennial fruit tree, belongs to family Sapindaceae and is indigenous to China. Its cultivation spread to the neighbouring countries of the south-east Asian region and other countries. The fruit of litchi is small, heart-shaped, conical or spherical in shape and pinkish red to bright-red in colour, with varying colour intensity in different cultivars. The pulp of fruit is crispy and grape-like in texture, very succulent and aromatic and is generally characterized by a sweet, acid, juicy and soft taste. Because of these reasons, litchi is highly prized in its fresh form as well as in different processed formulations. The total litchi production is about 24,00,000 tonnes from the net cultivated are of about 8,00,000 ha. The main litchi-producing countries are China, India and Thailand, whereas it is commercially grown also in many other countries. Of the total world litchi production, China and India contribute about 90%. Although litchi plants are long-lived, even then new plantings of this fruit are being done regularly, ensuring the continuous growth of production at the global level. In India, where this fruit can be grown in off season also in the southern states of Karnataka, Tamil Nadu and Kerala, the litchi production is about 7,37,200 tonnes from an area of 99,170 ha (Chandrakala et al., 2023).
 

Reasons for serious concern for food security in developing countries are, increasing food demand for the rapidly growing population, decline in productivity in high productive areas due to ‘Green revolution’ fatigue, and increasing vulnerability of agriculture to potential climate change. Climate scientists predict that global warming will make arable land in many developing countries less productive or unusable. Of all the parameters, abiotic environmental stresses contribute significantly to reduction in potential yield (Flowers and Yeo, 1995). Abiotic stresses arise from extremes of climate. The stress factors especially drought and salinity negatively effect plant growth and development, salt stress being one of the most serious environmental factors limiting productivity of crop plants (Ashraf, 1999). Therefore, extensive research into plant salt tolerance was carried out, with the aim of improving resistance of crop plants. With a large majority of the world’s population relying on crops like barley, maize and rice to survive, crop salt tolerance is globally important.

Understanding biology and genetics at molecular level has become very important for better understanding and manipulation of genome architecture and this creates new opportunities to streamline plant breeding. Breeding is simply defined as the selective mating of individuals of a population to isolate or combine desired morphological, physiological or genetic traits such as appearance, yield, and disease resistance. This is performed with the assistance of identifiable traits.

Molecular markers have contributed significantly in this respect and have been widely used in plant science in a number of ways, including genetic fingerprinting, diagnostics, identification of duplicates and selecting core collections, determination of genetic distances, genome analysis, identification of markers associated with desirable breeding traits which are useful in marker assisted breeding, genomics and development of transgenics. Use of these markers also significantly reduces breeding time and cycles required for crop improvement. This is reflected in the fact that complete molecular and genetic maps have now been constructed for at least 20 major crop species and many more have been characterized at less defined level and the genomes of a few model plant species were completely sequenced.

Introduction

India has very old and long tradition of flower cultivation. With changing life styles and increased urban affluence, floriculture has assumed a definite commercial status in recent times. Improved communication facilities have increased their availability in every part of the country. Floriculture in India is being viewed as a rapidly growing industry. In India, at present total area under flower crops is about 2,33,000 ha with production of about 17.29 lakh tonnes of loose flowers and 76732 lakh  number of cut flowers (NHB database, 2013).The important floricultural crops in the international flower trade are rose, carnation, chrysanthemum, gerbera, gladious, gypsophila, liastris, bird of paradise, orchids, anthurium, tulip, lilies, etc. All the flowers and ornamentals are the most perishable commodities needs special care during harvesting, handling, storage and transport. The losses can be both, quantitative as well as qualitative, which result in loss in terms of monitory value.

The word Cymbidium has been derived from a Greek word ‘Kumbos’ meaning ‘hole or cavity’ referring to the structure of the base of the lip. The genus consists of 44 known evergreen species in the family Orchidaceae, subfamily Vandoideae, tribe Cymbidieae and sub-tribe Cryptopodiinae. It is distributed from northwestern India to China, Japan, south through the Malay Archipelago to the north and east Australia. (DuPuy and Cribb, 1988). Cymbidiums have been for over hundred years one of the most famous orchids in commerce.

Vegetables are the most extensively utilized plants for  exploitation of heterosis for  development of hybrids. As the importance of heterosis breeding increased, male sterility proved an asset particularly in crops like onion and carrot which produce many but small sized flowers making hand emasculation tedious. Large scale hybrid seed production sometimes remains handicapped because of high labour cost, unavailability of trained labour at crucial times i.e. at blooming, bad weather conditions like continuous rains etc. The phenomenon of male sterility has always been of long term interest for  researchers of various disciplines of applied, strategic and basic sciences. It is of special interest for  plant breeders to produce more efficient and economic hybrid seed. Discovery of certain male sterile mutants which  eliminate more laborious operations of emasculation combined with various marker genes further facilitate identification of undesirable types even at seedling stage has widened the very basis of hybrid seed production. This technique has also reduced the total cost of hybrid seed production. Onion crop provides one of the rare examples of very early recognition of male sterility (Jones and Emsweller 1936), its inheritance and use in hybrid seed production (Jones and Clarke 1943). Since then male sterility is reported in fairly large number of crops including vegetables.

1. Introduction:- Understanding Good Laboratory Practices (GLP)
1.1 Definition and Core Principles of GLP
The Organisation for Economic Co-operation and Development (OECD) defines Good Laboratory Practice as “a managerial quality control system that governs the organizational process and the conditions under which non-clinical health and thus, environmental safety studies are well planned, executed/ performed, vigilantly monitored, properly recorded, reported, and finally archived”. This definition highlights that Good Laboratory Practices (GLP) is a comprehensive framework focused on ensuring the quality and integrity of the entire study lifecycle, rather than dictating the scientific validity of the research itself. The primary goal of GLP is to ensure process integrity and data traceability, which are essential for the regulatory acceptance of safety data for a variety of products. These products include pharmaceuticals, pesticides, cosmetics, veterinary drugs, food additives, feed additives, and industrial chemicals, all of which undergo non-clinical testing to assess their potential health and environmental risks. It is important to note that GLP principles specifically apply to these non-clinical studies and exclude research involving human subjects. The OECD Principles of GLP are internationally recognized and serve as the basis for GLP regulations in numerous countries worldwide. These principles outline the responsibilities of key personnel involved in non-clinical studies, including test facility management, the study director, study personnel, and quality assurance personnel. Furthermore, they establish minimum standards concerning the suitability of facilities and equipment to perform studies, as well as the necessity for comprehensive Standard Operating Procedures (SOPs) to guide all aspects of laboratory operations. The core principles of GLP encompass a wide range of elements critical to ensuring the quality and integrity of laboratory studies. These OECD principles of Good Laboratory Practices (GLP) include: the proper organization team and personnel staff members within the testing facility, the well establishment of a robust quality assurance programme, the better suitability and proper maintenance of various necessary facilities, the efficient management of apparatus, materials, and reagents, the characterization and control of test systems, the handling of test and reference items, the development and adherence to Standard Operating

ABSTRACT

Due to the exorbitant price of the popular gelling agent agar, Plant Tissue Culture (PTC) has gradually become a costly technique in recent times. So, alternative sources of agar needs to be popularized which at one hand should be able to meet all the positive properties of agar and on the other hand be cost effective as well. The seed husk obtained from Plantago ovata Forsk. is such an alternative which was successfully used as a gelling agent for media preparation in PTC experiments for in-vitro shoot and root development in Nicotiana tabacum L. and Ocimum gratissimum L. The nodal explants were grown in MS basal medium (MSo) supplemented with 3% sucrose and were gelled with progressive concentrations of isabgol husk to ascertain the optimum concentration for better survival and growth response, while 0.8% agar acted as a negative control. The response of the explants on media gelled with husk was compared to that of media gelled with 0.8% agar. It was inferred that 0.3% husk in the medium was optimum for best growth response which was comparable to that of agar. Consequently, the estimated cost of a liter of MSo reduced by almost Rs.61.15/- (compared to Himedia’s Agar) to Rs.177.57/- (compared to Sigma’s Agar) after using isabgol seed husk as a gelling agent in media preparation.