eChapters

Spices are defined as a strongly flavored or aromatic substance of vegetable origin, obtained from tropical plants, commonly used as a condiment. India is known as ‘land of spices’. Chilli (Capsicum annuum L.) also known as hot peppers and Capsicums also referred as bell peppers or sweet peppers belong to the genus Capsicum which is a member of the Solanaceae family. The native home of chilli is considered to New Mexico with secondary origin at Guatemala. They are known from pre-historic remains of Peru. Chilli was introduced into India by Portuguese in 16th century. Chilli is considered as one of the major commercial crops of the world. Both green and dried chillies are the important components of our routine diet. Chilli also contains vitamin A, C and E. It will give the required pungency, colour, taste and flavour to our dishes. The presence of capsaicin in chilli is responsible for its pungency and it has medicinal value. The extracted capsaicin is used in pain balms, cosmetics and medicines related to heart diseases. Capsanthin, a pigment in chilli is used for natural colouration to Jams, Jellies and squashes, since it is a natural pigment and no harmful or side effects on human health. Oleoresin is used in pain balms, vapours, etc. Hence, there is lot of demand for chilli oleoresin in the world market. The largest producer of chillies in the world is India contributing 52 per cent of world production, accounting for 11.67 lakh tonnes of production from an area of 7.5 lakh ha with 1550 kg/ha of productivity followed by Nigeria (17%), China (16%), Peru and Bangladesh (6%). India exported 1.8 million tonnes of chilli during 2009 (Reddy, 2010).

ABSTRACT

It is essential to prospect wild Zingiber species for soft rot resistance, since the cultivated ginger is monotonously susceptible to this disease. We evaluated the genetic diversity of Zingiber species and their response to Pythium aphanidermatum, the causal agent of soft rot. Diversity of 21 cultivars and three wild accessions of Z. officinale, 84 accessions of five wild species: Z. zerumbet, Z. cernuum, Z. neesanum, Z. purpureum and Z. wightianum and one accession of Hedichium flavescens were examined using AFLP markers. No polymorphism was detected at more than 500 AFLP loci screened in ginger cultivars, which to our knowledge, is the most extreme case of genetic narrowness in a species. Analysis revealed high variability in Z. cernuum and relatively low variations in other species. Strong correspondence was evident between the genetic diversity of a species and its mode of propagation. Selected accessions of Zingiber species were inoculated with Pythium zoospores and the disease progression was scored. The response to Pythium varied from total susceptibility in Z. cernuum and Z. officinale to hardy behavior among Z. zerumbet accessions. The present study provided an estimate of genetic diversity of Zingiber species common in Kerala. Species relationships and population genetic structure were discussed. The monotypic nature of ginger cultivars may hinder the possibility of their identification based on their nucleic acid profiles. The present study identified Z. zerumbet as a potential source of soft rot resistance, which will trigger off the application of genomics tools to access this trait in order to genetically engineer soft rot resistance in ginger.

African leaf vegetables (ALVs) are vegetable species utilized for their leaves and have become a part of the African tradition. This is a dynamic concept that is changing with the African culture and the term ALV embraces not only indigenous, but also indigenised species. These species are very nutritious, and contain high levels of pro-vitamin A and iron. ALVs are often more hardy than the introduced “modern” vegetables. The important role that ALVs have played in African livelihood strategies has been ignored, especially in the last half of the twentieth century. This led to ALVs being seen as a low-status crop, but this perception  started to change during the last two decades. ALVs are entering the commercial market and regaining its equal status in many African countries. ALVs are mostly used as an ingredient in relishes and soups to accompany starchy staples and sometimes meat.

ALVs include species cultivated in farmer fields and home gardens, like  pumpkins and cowpeas, and species harvested from  wild and fallow lands, like eru and cleome. Certain cultivated species have only recently been domesticated after a long history of wild harvesting.  Currently, ALVs are mainly cultivated using traditional farm practices with almost no African countries having formal seed systems. After years of neglect by formal research, more and more research institutions are initiating research on ALVs. Amaranth, cleome, corchorus, cowpea, pumpkins, nightshades, african eggplant, moringa, ethiopian kale, roselle, bitterleaf, taro, slenderleaf and water spinach are listed as the most prominent ALVs in Africa.

1. Introduction

The Aquilaria malaccensis (Agarwood) is an Appendix II species of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES, 1994). The species is also red listed under International Union for Conservation of Nature and Natural Resources (IUCN) and is classified as ‘Vulnerable’ globally but ‘Critically Endangered’ in India (Oldfield et al., 1998; IUCN, 2009) and almost ‘Extinct in wild’ in Assam (Anon., 2003; Saikia and Khan, 2012a). The species due to its multipurpose uses is overexploited which is threatening its existence from the wild (Lee and Mohamed, 2016; Talucder et al.,2016). There are 21 reported Aquilaria species, of which 13 are fragrant resin producers, while the rest are yet to be studied (Lee and Mohamed, 2016; Talucder et al.,2016). Eleven of the domesticated species are A. malaccensis, A. crassna, A. subintegra, A. hirta, A.rostrata, A. beccariana, A. filaria, A. khasiana, A.microcarpa, A. grandiflora and A. sinensis (Zuhaidi, 2016). Species reported from Asia viz., A. apiculata, A. baillonii, A. banaense, A. beccariana, A. brachyantha, A. cumingiana, A. filaria, A. hirta, A. khasiana, A. malaccensis, A. microcarpa, A. rostrata, A. sinensis, A. subintegra and A. crassna are known to produce essential oils (Sulaiman et al., 2015). Only three species i.e. Aquilaria khasiana, A. macrophylla and A. malaccensis has centre of origin in India with A. macrophylla endemic to Nicobar Islands (Anon., 2003; Giri, 2003) and A. khasiana endemic to Khasi Hills of Meghalaya and surroundings (Kanjilal et al., 1982), while A. malaccensis is native to evergreen rain forests in the foothills of northeastern India (Chakrabarty et al., 1994; Saikia and Khan, 2012b) and West Bengal (Barden et al., 2000).

Promotion of perennial crops is a good alternative for food security since they offer more consistant, year-round production without any break. Agathi and Chekkurmanis are the two nutritive perennial greens that can be easily grown in hot and humid tropical climate.

Over 85% of the rural population in India is dependent on agriculture for their livelihood. About 50% of them being poor, most of their earnings are spent on meeting their basic needs, particularly food. With the increasing population over the last five decades, their per capita share of land and water resources has reduced substantially. As a result, rural people are faced with the problem of unemployment.

Inspite of agriculture being a major source of livelihood, the productivity as well as profitability in agriculture have been significantly low in the country. While the productivity can be attributed to illiteracy, lack of awareness, poor dissemination of technology, inadequate investment in agricultural inputs and poor communication and information services, lack of profitability is mainly due to inadequate and inefficient infrastructure required for forward and backward integration, poor post harvest and processing facilities and poor connectivity with market, resulting in exploitation by large number of middlemen. Traditionally, the farmers have been dependent on the Agricultural Extension Agencies of the State Government for information, input supply and marketing services. Over the years, these agencies are not being able to cope up with the growing responsibilities and specific needs of the farmers. To overcome this problem, Farmers’ Cooperatives have been promoted to supply various agricultural inputs and organise the marketing of farm produce. Although, this was an excellent concept, most of these cooperatives could not carry out the work economically and efficiently due to lack of commitment from the elected leaders and unfair trade practices by the competing traders. The farmers, not realising the inability of the extension agencies, are still dependent on the Government and external agencies to help them in managing their agri-business.

Introduction
Horticulture is distinct from other economic sectors as its output is heavily reliant on weather conditions. The degree of success of horticultural production and economics is decided to a large extent by how well weather conditions corresponding to the crop’s optimal requirements are best utilized to raise the crops. Also, how successfully adverse weather circumstances, such as moisture, thermal, wind, radiation and biotic stresses were handled to minimize adversity. It is well understood that variability in weather elements (particularly rainfall and temperature) is the primary cause of inter-annual variability in crop output. The onset of weather extremes (dry spells, droughts, floods, heat waves, and hailstorms) has a negative impact on horticultural yield, resulting in a low level of productivity. Furthermore, weather can have an impact on output at multiple levels (vegetative stage, harvest, transportation, storage, etc.). Flooding, droughts, heat waves, cold spells, hailstorms, and other natural calamities pose significant risks to farms. These natural catastrophes can lead to lower yields, leaving uninsured farmers with little income for the season. Farmers often plant low-risk, low-return crops (such as early maturing varieties that are drought-resistant) to deal with erratic weather rather than investing in more profitable crops that are more sensitive to weather fluctuations and extremes. Thus, atmospheric stress has significant impacts on the food ecosystem (Bal and Minhas, 2017; MANAGE, 2018).

Introduction
Management of abiotic stresses in fruit orchards are essential and key issue for sustaining productivity. Worldwide fruit consumption has increased significantly because of the new recognitions of its broad relevance for health, nutrition and livelihood (Lansky and Newman, 2007). Fruits like pomegranate, grapes, dragon fruit, mango and sapota are being grown in tropical humid to dry as well as arid and semi-arid climates of India, especially in states like Maharashtra, Karnataka, Tamil Nadu, Andhra Pradesh, and Gujarat. Significant cultivars such as Bhagwa of pomegranate, Hapus, Kesar, Langra, Banganpalli, Totapuri of mango and Kallipati, Cricket ball of Sapota are being dominantly grown in these areas. Area under pomegranate was 2.62 lakh ha in 2018-19, producing 30.04 lakh MT at a productivity of 11.58  ha-1 with an export of 80.5 thousand MT during 2019-20 (Maity et al., 2020). The estimated area under grapes, mango, pomegranate and sapota in India is 0.14, 2.29, 0.26, 0.08 million ha, respectively whereas production estimates are reported to be 31.25, 204. 44, 23.15 and 10.03 million MT, respectively (NHB 2019-20 2nd estimate). Besides area of dragon fruit in India is rapidly expanding due to its high nutritional content, antioxidant properties, and market opportunities, covering around 20 thousand ha with annual production of 0.3 million MT. Despite the fact that >15 species of Hylocereus have been reported; only 3 to 4 are under cultivation. Among these, white flesh (H. undatus) occupied more than half of the area. However, the current premium pricing and consumer demand for pink flesh may shift cultivation to H. polyrhizus. Furthermore, species with yellow peel and white flesh (H. megalanthus) is now being cultivated. In this context, the issuances of agro-advisory services to farmers are essential for adopting time bound management protocols (Adak et al.,2018a). The importance of this scientific management is significantly linked with the fruit business both in domestic market and foreign export. Therefore, it is of great importance for advisory, training, farmers-scientific interaction and other human resource management programmes to sensitize and develop trained man power sufficiently which contributes to the horticultural growth and development. In this backdrop, fruit orchards and their scientific management is discussed in this chapter with special reference to abiotic and biotic stress management in Pune region of Maharashtra.

Introduction
Mangoes (Mangifera Indica L.), often referred to as the “king of fruits,” are celebrated for their rich flavour, nutritional value, and versatility in culinary applications (Swaroop et al., 2018). Globally, mangoes hold a significant economic and cultural position. Mangoes are grown in over 90 countries, with India, China, and Thailand being leading producers. They contribute
significantly to the agricultural economies of these and other tropical and subtropical regions (Makarabbi, 2023). Mangoes are a major export commodity for many countries, generating substantial foreign exchange. For instance, India’s mango exports account for a sizable share of the global mango market, valued at millions of dollars annually. In many developing countries, mango farming supports millions of smallholder farmers, providing employment and a source of income (Thakor, 2019). Mangoes are enjoyed in numerous forms— fresh, dried, as juices, pickles, and chutneys. They are also a key ingredient in various traditional dishes and desserts across different cultures. Mangoes play a central role in several cultural festivals and rituals, symbolizing prosperityand good fortune in countries like India and the Philippines. Mangoes are rich in essential vitamins, minerals, and antioxidants, including vitamins A and C, folate, and fiber. Their consumption is associated with various health benefits, such as improved immunity, digestive health, and skin care (Yahia et al., 2023). While mangoes offer significant benefits, their cultivation faces several challenges, primarily due to pest infestations that can severely impact both yield and fruit quality. Effective management of these pests is crucial to ensure sustainable mango production (Singh and Baradevanal, 2021).

ABSTRACT

Chilli is the universal spice of Kerala, known for its pungency, flavour and colour of fruits. Of the five domesticated species of Capsicum, C. annuum is the most extensively cultivated one. Bell pepper (C. annuum L.) is mainly used as a vegetable, because of its low pungency and good flavour. It is used as raw in salads, and also in cooked form. Application of biotechnological tools in this crop for improving the quality, shelf life and tolerance of the crop to stress conditions has been limited. This is mainly due to lack of an efficient regeneration system. We reported a viable protocol for the regeneration of whole plant from the hypocotyl earlier. We also succeeded in developing a protocol for genetic transformation of bell pepper using Agrobacterium tumefaciens. The strain EHA 105 containing gus intron and npt II was used for co-cultivation. The dilution of bacterial culture and time of infection were standardised. This was followed by killing the bacterial cells using cefotaxim @ 250ppm. Transformants were selected on MS medium supplemented with kanamycin. Staining of the regenerants with X-gluc revealed presence of blue spots, confirming transformation. This protocol could be exploited for improving the commercial traits of bell pepper by incorporating desirable genes through Agrobacterium mediated transformation.

ABSTRACT

Astudy was undertaken with the main objective to standardise the Agrobacterium mediated genetic transformation technique in Dendrobium Sonia 17. Protocorms and protocorm like bodies (PLBs) of Dendrobium orchid were transformed by Agrobacterium tumefaciens strains LBA4404 and EHA105 harbouring the plasmids pCAMBIA2301, pCAMBIA1301 and PBI121. The PLBs were generated in vitro by culturing the shoot apices in half strength MS medium. The protocorms were obtained by culturing the seeds in vitro. The sensitivity of orchid tissues to various antibiotics viz., ampicilin, rifampicin, cefotaxime, carbenicillin, kanamycin and hygromycin was evaluated to use the antibiotics as selection agents. The sensitivity was studied in detail by scoring the tissues, based on the morphological changes as those remaining fully green, partially discoloured, bleached, completely turning brown and dead. The various requirements for transformation viz., the type and size of explant, infection time, wounding, co-cultivation and incubation period, concentration of acetosyringone required, elimination of bacteria, and the selection of transformants were optimised to establish a successful transformation system. The pre- cultured explants were mixed thoroughly by gentle swirling with the bacterial cells obtained by centrifuging the bacterial suspension, and also with the bacterial suspension diluted to 1/5 with fresh medium. Then the explants were cocultivated with the bacteria for 2 days at 28°C. The transformation was performed through two consecutive stages of cultivation, the first stage on half strength MS medium containing cefotaxime and subsequent stage on selection medium containing kanamycin. Initially recovered PLBs following co-cultivation, were allowed for further selection with kanamycin. New PLBs and leaves were formed from the transformed PLBs when they were regenerated under kanamycin selection. Successful transformants were obtained with a maximum transformation efficiency of 3.0 per cent with the addition of acetosyringone 100 mM. Out of the kanamycin resistant PLBs 69.23 per cent were GUS positives. The newly formed PLBs and leaves when subjected to histochemical GUS assay indicated successful transformation and it was detected as distinct blue spots on the PLBs after 24’ hours. The PLBs which were not co-cultivated with Agrobacterium were green in colour. The transformation were further confirmed by PCR analysis.

ABSTRACT

Solanum trilobatum Linn. (Solanaceae) was investigated for the induction of tumor using a soil borne bacterium, Agrobacterium tumefaciens. Explants like stem segments and nodes were infected with bacterium. Tumors were induced from nodal explants within 10 days of infection. These explants were then transferred to MS basal medium and their growth was observed to be independent of exogenous supply of phytohormones. The growth rate of transformed callus was 10-fold as compared to control. They are used as a promising source of secondary metabolites of medicinal significance.

ABSTRACT

Rapid optimization and improvement of papaya transformation necessacitates, a quick, non-destructive marker, which permits early detection of transformed tissue. We assessed the ability of a modified version of the green fluorescent protein gene (GFP) to act as the reporter. Transgenic papaya (Carica papaya L.) plants were developed using immature zygotic embryo as the expl ants and Agrobacterium tumefaciens as the transformation inducer. GFP gene (pBIN35Smgfp-ER) under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter was used as the reporter gene. Media P (modified MS basal medium with B vitamins supplemented with picloram 1 mg/l) and BN (modified MS basal medium with B vitamins supplemented with BAP: NAA 1:0.1 mg/l) supplemented with kanamycin (50 mg/l) were used for regeneration of transgenic papaya under selection pressure. GFP gene expression was detected at the juvenile stages viz. at early stages of somatic embryogenesis. 35S promoter driven GFP gene activity was also detected in the epidermis and the vascular tissues of the leaf petiole. Visualization under microscope and subsequent DNA analysis (Southern hybridization) showed that kanamycin resistant plants carried and expressed the GFP transgene. Expression of the GFP as green fluorescence served as a useful non-destructive indicator of successful transformation.

Introduction

A number of experts have recognised three different stages in the march of man on the earth. These consist of: (i) When the civilization is dominated by the forests, (ii) When the civilization is overcoming the forest and (iii) When the civilization is dominating obstacles caused by forests. Now a days, Indian Himalayas including that of Jammu and Kashmir is passing through the third stage i.e., to the point of destroying itself in the process.

Deforestation’s relentless march is stripping the Jammu and Kashmir Himalayas bare of its forest cover. From the majestic coniferous forests of Pir Panjal Himalayas to the deciduous and mixed canopy of subtropical forests the story is tragically the same i.e.. the indiscriminate destruction of forests that could turn the state into vast and inhospitable waste land. Hence to sustain and prosper the civilization, the forests must be saved from being further destroyed.

To reduce or to minimize the deforestation, the Social Forestry Project and Agroforestry System came to fore. The primary objective of Social Forestry is to increase supplies of fuel wood and fodder in rural area through plantation on community waste lands, degraded forest lands and on private lands through farm forestry (Patnaik, 1995-96). The latter i.e., Agroforestry is now much more widely used subject than the mere production of wood and food. In the changing scenario of the environmental degradation and ecological imbalance as a result of degradation of the forest resources of the country, agroforestry consists of inviting the prime attention of forests for upliftment of both environment and ecology vis-a-vis to enhance agricultural/horticultural and livestock production. In fact, agroforestry came as a new’ name during the year 1978. Since then, the Indian Council of Agricultural Research (ICAR), New Delhi, has developed a vast network of agroforestry in all 36 centres of Agroclimatic Zones (Tejwani, 2008). However, Dr Tejwani started working with agroforestry during 1957 on fodder fuel plantations and trees on farm boundaries. Hence, agroforestry must be included in the agriculture.

The Government of India, during 1980s, recognized the necessity to improve the latex yield productivity in the traditional rubber growing regions and also expand these plantations to non-traditional regions to meet the ever increasing demands of natural rubber in India (Sethuraj et al., 1989). Agrometeorological studies were initiated during early 1986 with the appointment of a Scientist (Agrometeorology). To begin with, scientific issues were identified as: (a) delineate the potential non-traditional regions for expansion of rubberplantations, (b) estimate the impact of climatic conditions, especially abiotic  stress conditions on productivity, (c) quantify influence of meteorological factors on the physiological responses towards plant growth and yield attributes, (d) estimate the irrigation requirements to mitigate the drought conditions, and (e) develop weather based predictive models for `Abnormal leaf fall’ disease of rubber plantations. An attempt is made to present synthesis of accomplishments during 1986 to 1992 period of my association with the Rubber Research Institute of India (RRII), Kottayam.

1. Introduction

Zinc is an important micro-nutrient for human population and its deficiency can cause major diseases, mortality and thus becomes a major challenge in public health care systems of the developing nations. It was estimated that a major part of population is affected with deficiency of zinc (Combs, 2001) which causes lower growth, abnormal or decrease in infant birth, lowers the energy of body, decreases the power to lower the deadly infections, increase the mortality rate in children etc. (Gibson 2012; Krebs et al., 2014). Zinc also catalyzes various enzymes such as RNA polymerase, superoxidase dismutase and helps in working efficiency of cellular proteins. 

Selection is an important activity in all the breeding programmes. Different types of selections i.e. mass selection, progeny selection and cyclic selection etc. are used depending on the mode of pollination of the crop species, the predominant gene action and the breeding objectives. The efficiency of selection largely depends on the extent of genetic variability present in the population and the heritability of the character. Selection is more effective for character with high heritability than those having low heritability. Breeding for higher or better yield is the breeding objective for most of the breeding programmes, but it has low heritability. Yield is considered to be complex or super character which is influenced by many components or contributing traits both in positive and in negative directions. Due to the fact that it has low heritability, selection is done for the yield contributing traits so that yield may be increased through selection. Prior to selection for yield contributing traits, it is always beneficial to study the extent and type of relationship between the character and yield. There are different procedures which aid in the isolation of superior yielding genotypes from genetically variable populations by providing information on indirect selection for yield. These methods are discussed in the following content.

For ages, man has consumed alcohol to forget his sorrows, to lead him into the realm of happiness. John Keats (1795 -1821), the great romantic English poet, listened to the full-throated music of a nightingale and wanted to become one with that moment of happiness. He thought of taking 'a beaker full of the warm south, of vintage, that has been cooled a long age in the deep delved earth⁷, drinking which he could ' leave the world unseen', and fade away into the dim forest with the singing bird. But Keats was romanticizing the effect of wine on the human mind. A moment later, he decided to give up the idea of taking the assistance of Bacchus and his leopards and switched over to reaching the bird and its music on 'the viewless wings of poesy⁷. This was definitely a wise decision because the alleged euphoric effect on alcohol is a myth. In fact alcohol dulls the brain and retards its activity.

Alcohol is basically a drug. Man consumes it more than any other drug. No form of alcohol, however mild, whether wine or beer or cider, can do good to the human system and health.

It is fallacy to believe that alcohol stimulates the brain. In reality, alcohol depressed the brain. It slows down various centers of control. When alcohol is swallowed, it goes into the stomach where a fraction of the dose enters the blood stream through the blood vessels of the stomach walls. From the stomach, alcohol has to pass into the small intestine. The carbon dioxide in soda water accelerates this flow of alcohol from the stomach into the intestines. This is what makes whisky and soda more dangerous than whisky and water.