eChapters

Old and Modern biotechnology
Agriculture has evolved since the dawn of human civilization, first as a means to guarantee food supply and then, as a source of family income and improved profitability. Domestication of plants and animals that were found in the wild combined with gradual, long-term changes in their quality and quantity were the first signs of what is now collectively termed “Agriculture”. At that time, agriculture replaced the former nomadic habit of food collection for immediate consumption. Domestication of plants and animals, followed by food storage, coincided most probably unintentionally, with the growth of microorganisms. Thus, was born the classical food fermentation, the earliest known application of biotechnology, focusing on the use of microorganisms to produce food products.
Gradual improvements in agricultural techniques, domestication of additional plant and species from the wild, step-by-step selection of better performing and more adapted genotypes, along with intuitive breeding continued at a slow pace. Mendel’s discoveries and laws revolutionized genetics, and led at the beginning of the 20th century, to planned and controlled breeding experiments. Breeding or the “old biotechnology” was harnessed as a most successful tool, resulting in better crops and farm animals, and “Green Revolution” of the 1960s was achieved. Many achievements are still being successfully implemented since then.

Introduction
In India, the government policy regarding the import and export of processed fruits is primarily regulated by the Agricultural and Processed Food Products Export Development Authority (APEDA), which requires exporters to register with them to export notified processed food items, including processed fruits, and implement various export promotion schemes to facilitate this trade; the overall goal is to boost the export of agricultural and processed food products through the Agriculture Export Policy (AEP).
1. Key features ofAgriculture Export Policy (AEP)
i. Governing body: APEDA, established under the Agricultural and Processed Food Products Export Development Authority Act, 1985.
ii. Registration required: All exporters of processed fruits are required to register with APEDA to export their products.

Introduction
Horticulture is distinct from other economic sectors as its output is heavily reliant on weather conditions. The degree of success of horticultural production and economics is decided to a large extent by how well weather conditions corresponding to the crop’s optimal requirements are best utilized to raise the crops. Also, how successfully adverse weather circumstances, such as moisture, thermal, wind, radiation and biotic stresses were handled to minimize adversity. It is well understood that variability in weather elements (particularly rainfall and temperature) is the primary cause of inter-annual variability in crop output. The onset of weather extremes (dry spells, droughts, floods, heat waves, and hailstorms) has a negative impact on horticultural yield, resulting in a low level of productivity. Furthermore, weather can have an impact on output at multiple levels (vegetative stage, harvest, transportation, storage, etc.). Flooding, droughts, heat waves, cold spells, hailstorms, and other natural calamities pose significant risks to farms. These natural catastrophes can lead to lower yields, leaving uninsured farmers with little income for the season. Farmers often plant low-risk, low-return crops (such as early maturing varieties that are drought-resistant) to deal with erratic weather rather than investing in more profitable crops that are more sensitive to weather fluctuations and extremes. Thus, atmospheric stress has significant impacts on the food ecosystem (Bal and Minhas, 2017; MANAGE, 2018).

Introduction
Management of abiotic stresses in fruit orchards are essential and key issue for sustaining productivity. Worldwide fruit consumption has increased significantly because of the new recognitions of its broad relevance for health, nutrition and livelihood (Lansky and Newman, 2007). Fruits like pomegranate, grapes, dragon fruit, mango and sapota are being grown in tropical humid to dry as well as arid and semi-arid climates of India, especially in states like Maharashtra, Karnataka, Tamil Nadu, Andhra Pradesh, and Gujarat. Significant cultivars such as Bhagwa of pomegranate, Hapus, Kesar, Langra, Banganpalli, Totapuri of mango and Kallipati, Cricket ball of Sapota are being dominantly grown in these areas. Area under pomegranate was 2.62 lakh ha in 2018-19, producing 30.04 lakh MT at a productivity of 11.58  ha-1 with an export of 80.5 thousand MT during 2019-20 (Maity et al., 2020). The estimated area under grapes, mango, pomegranate and sapota in India is 0.14, 2.29, 0.26, 0.08 million ha, respectively whereas production estimates are reported to be 31.25, 204. 44, 23.15 and 10.03 million MT, respectively (NHB 2019-20 2nd estimate). Besides area of dragon fruit in India is rapidly expanding due to its high nutritional content, antioxidant properties, and market opportunities, covering around 20 thousand ha with annual production of 0.3 million MT. Despite the fact that >15 species of Hylocereus have been reported; only 3 to 4 are under cultivation. Among these, white flesh (H. undatus) occupied more than half of the area. However, the current premium pricing and consumer demand for pink flesh may shift cultivation to H. polyrhizus. Furthermore, species with yellow peel and white flesh (H. megalanthus) is now being cultivated. In this context, the issuances of agro-advisory services to farmers are essential for adopting time bound management protocols (Adak et al.,2018a). The importance of this scientific management is significantly linked with the fruit business both in domestic market and foreign export. Therefore, it is of great importance for advisory, training, farmers-scientific interaction and other human resource management programmes to sensitize and develop trained man power sufficiently which contributes to the horticultural growth and development. In this backdrop, fruit orchards and their scientific management is discussed in this chapter with special reference to abiotic and biotic stress management in Pune region of Maharashtra.

Introduction
Mangoes (Mangifera Indica L.), often referred to as the “king of fruits,” are celebrated for their rich flavour, nutritional value, and versatility in culinary applications (Swaroop et al., 2018). Globally, mangoes hold a significant economic and cultural position. Mangoes are grown in over 90 countries, with India, China, and Thailand being leading producers. They contribute
significantly to the agricultural economies of these and other tropical and subtropical regions (Makarabbi, 2023). Mangoes are a major export commodity for many countries, generating substantial foreign exchange. For instance, India’s mango exports account for a sizable share of the global mango market, valued at millions of dollars annually. In many developing countries, mango farming supports millions of smallholder farmers, providing employment and a source of income (Thakor, 2019). Mangoes are enjoyed in numerous forms— fresh, dried, as juices, pickles, and chutneys. They are also a key ingredient in various traditional dishes and desserts across different cultures. Mangoes play a central role in several cultural festivals and rituals, symbolizing prosperityand good fortune in countries like India and the Philippines. Mangoes are rich in essential vitamins, minerals, and antioxidants, including vitamins A and C, folate, and fiber. Their consumption is associated with various health benefits, such as improved immunity, digestive health, and skin care (Yahia et al., 2023). While mangoes offer significant benefits, their cultivation faces several challenges, primarily due to pest infestations that can severely impact both yield and fruit quality. Effective management of these pests is crucial to ensure sustainable mango production (Singh and Baradevanal, 2021).

It sounds like you're interested in agro-animal-based yarns, natural dyes, and weaves. These are fascinating topics that blend traditional craftsmanship with sustainable practices. Here are some insights and examples:

1. Agro-animal-based Yarns

a. Wool: Wool is one of the most common agro-animal-based yarns, sourced from sheep. It's known for its warmth, durability, and natural elasticity.

b. Silk: Silk is produced from silkworms, particularly the mulberry silkworm. It's valued for its smooth texture and luxurious appearance.

c. Mohair and Cashmere: These fibers come from goats (Angora and Cashmere goats, respectively). Mohair is known for its luster and durability, while cashmere is prized for its softness and warmth.

2. Natural Dyes

a. Indigo: Derived from the indigo plant, it produces shades of blue and is one of the oldest natural dyes used globally.

ABSTRACT

Chilli is the universal spice of Kerala, known for its pungency, flavour and colour of fruits. Of the five domesticated species of Capsicum, C. annuum is the most extensively cultivated one. Bell pepper (C. annuum L.) is mainly used as a vegetable, because of its low pungency and good flavour. It is used as raw in salads, and also in cooked form. Application of biotechnological tools in this crop for improving the quality, shelf life and tolerance of the crop to stress conditions has been limited. This is mainly due to lack of an efficient regeneration system. We reported a viable protocol for the regeneration of whole plant from the hypocotyl earlier. We also succeeded in developing a protocol for genetic transformation of bell pepper using Agrobacterium tumefaciens. The strain EHA 105 containing gus intron and npt II was used for co-cultivation. The dilution of bacterial culture and time of infection were standardised. This was followed by killing the bacterial cells using cefotaxim @ 250ppm. Transformants were selected on MS medium supplemented with kanamycin. Staining of the regenerants with X-gluc revealed presence of blue spots, confirming transformation. This protocol could be exploited for improving the commercial traits of bell pepper by incorporating desirable genes through Agrobacterium mediated transformation.

ABSTRACT

Astudy was undertaken with the main objective to standardise the Agrobacterium mediated genetic transformation technique in Dendrobium Sonia 17. Protocorms and protocorm like bodies (PLBs) of Dendrobium orchid were transformed by Agrobacterium tumefaciens strains LBA4404 and EHA105 harbouring the plasmids pCAMBIA2301, pCAMBIA1301 and PBI121. The PLBs were generated in vitro by culturing the shoot apices in half strength MS medium. The protocorms were obtained by culturing the seeds in vitro. The sensitivity of orchid tissues to various antibiotics viz., ampicilin, rifampicin, cefotaxime, carbenicillin, kanamycin and hygromycin was evaluated to use the antibiotics as selection agents. The sensitivity was studied in detail by scoring the tissues, based on the morphological changes as those remaining fully green, partially discoloured, bleached, completely turning brown and dead. The various requirements for transformation viz., the type and size of explant, infection time, wounding, co-cultivation and incubation period, concentration of acetosyringone required, elimination of bacteria, and the selection of transformants were optimised to establish a successful transformation system. The pre- cultured explants were mixed thoroughly by gentle swirling with the bacterial cells obtained by centrifuging the bacterial suspension, and also with the bacterial suspension diluted to 1/5 with fresh medium. Then the explants were cocultivated with the bacteria for 2 days at 28°C. The transformation was performed through two consecutive stages of cultivation, the first stage on half strength MS medium containing cefotaxime and subsequent stage on selection medium containing kanamycin. Initially recovered PLBs following co-cultivation, were allowed for further selection with kanamycin. New PLBs and leaves were formed from the transformed PLBs when they were regenerated under kanamycin selection. Successful transformants were obtained with a maximum transformation efficiency of 3.0 per cent with the addition of acetosyringone 100 mM. Out of the kanamycin resistant PLBs 69.23 per cent were GUS positives. The newly formed PLBs and leaves when subjected to histochemical GUS assay indicated successful transformation and it was detected as distinct blue spots on the PLBs after 24’ hours. The PLBs which were not co-cultivated with Agrobacterium were green in colour. The transformation were further confirmed by PCR analysis.

ABSTRACT

Rapid optimization and improvement of papaya transformation necessacitates, a quick, non-destructive marker, which permits early detection of transformed tissue. We assessed the ability of a modified version of the green fluorescent protein gene (GFP) to act as the reporter. Transgenic papaya (Carica papaya L.) plants were developed using immature zygotic embryo as the expl ants and Agrobacterium tumefaciens as the transformation inducer. GFP gene (pBIN35Smgfp-ER) under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter was used as the reporter gene. Media P (modified MS basal medium with B vitamins supplemented with picloram 1 mg/l) and BN (modified MS basal medium with B vitamins supplemented with BAP: NAA 1:0.1 mg/l) supplemented with kanamycin (50 mg/l) were used for regeneration of transgenic papaya under selection pressure. GFP gene expression was detected at the juvenile stages viz. at early stages of somatic embryogenesis. 35S promoter driven GFP gene activity was also detected in the epidermis and the vascular tissues of the leaf petiole. Visualization under microscope and subsequent DNA analysis (Southern hybridization) showed that kanamycin resistant plants carried and expressed the GFP transgene. Expression of the GFP as green fluorescence served as a useful non-destructive indicator of successful transformation.

India’s diverse climate and geography provide a favorable environment for growing a wide range of fruit-type vegetables, including eggplant, tomato, okra, and chillies. These crops are not only essential for food security but also contribute significantly to the country’s agricultural economy. To optimize the production and productivity of these crops, it is crucial to understand their agroclimatic requirements.
Fruit-type vegetables have specific temperature requirements for growth and development. For instance, eggplant and okra require warm temperatures between 20-30°C (68-86°F) for optimal growth, while tomatoes prefer a slightly cooler temperature range of 15-25°C (59-77°F). Chilli peppers, on the other hand, thrive in temperatures between 20-35°C (68-95°F). Adequate moisture is also essential for the growth and development of fruit-type vegetables. Eggplant, okra, and chilli peppers require consistent moisture, especially during the fruiting stage, while tomatoes require a slightly drier soil condition. The ideal annual rainfall for these crops ranges from 600-1,200 mm, with supplemental irrigation provided during periods of drought.
Weather conditions, such as sunlight, wind, and frost, also play a crucial role in the growth and development of fruit-type vegetables. Most fruit-type vegetables require full sun to produce well, while excessive wind can damage plants and reduce yields. Frost, on the other hand, can be detrimental to these crops, and frost-free periods are essential for optimal growth. India‘s diverse
climate and geography result in regional variations in agroclimatic conditions. For instance, the Indo-Gangetic Plains, which cover parts of Uttar Pradesh, Bihar, and West Bengal, have a subtropical climate with hot summers and cold winters. In contrast, the Deccan Plateau, which covers parts of Maharashtra, Karnataka, and Telangana, has a tropical climate with warm summers and mild winters. Understanding these regional variations is essential for optimizing the production and productivity of fruit-type vegetables. The agroclimatic conditions suitable for growing different fruit type vegetables are given in this chapter.

Allium vegetables, which include onions, garlic, and shallots, are a fundamental part of Indian cuisine and play a vital role in the country’s agricultural sector (Fig. 7.1). Here’s an overview of their importance, area, and production in India:
• India is the second-largest producer of onions in the world, accounting for over 20% of global production. Onions are grown across various states, with Maharashtra, Madhya Pradesh, and Karnataka being the top producers. The country produces over 19 million tons of onions annually, with a significant portion being consumed domestically.
• Garlic is another essential Allium crop in India, with the country producing around 1.4 million tons annually. The major garlic-producing states in India are Madhya Pradesh, Gujarat, and Maharashtra.
• Shallots are also grown in India, particularly in the states of Maharashtra, Gujarat, and Tamil Nadu. The country produces around 0.7 million tons of shallots annually.
• The importance of Allium vegetables in India extends beyond their culinary uses. They are a significant source of income for millions of farmers across the country. The Allium crop industry also provides employment opportunities in rural areas, contributing to the country’s economic growth.

Brassica is a genus of plants belonging to the mustard family (Brassicaceae) and includes some of the most widely cultivated and consumed vegetables globally. The Brassica group encompasses a diverse range of cool-season crops, including broccoli, cauliflower, cabbage, kale, and mustard, among others (Fig. 8.1). These vegetables are not only rich in nutrients, fiber, and antioxidants but also play a vital role in human health, food security, and sustainable agriculture.
• India is one of the largest producers of Brassica vegetables, with a  significant portion of the crop being cultivated in the Indo-Gangetic plains. According to the Indian Horticultural Database, Brassica vegetables are grown on approximately 2.5 million hectares of land, with a total production of over 30 million metric tons annually. The major Brassica-producing states in India include Maharashtra, Madhya Pradesh, Gujarat, Uttar Pradesh, and West Bengal.
• The importance of Brassica vegetables extends beyond their nutritional value and economic significance. They are also an integral part of Indian cuisine and culture, with various regional and traditional recipes featuring these vegetables as main ingredients. Furthermore, Brassica crops are a vital component of crop rotation and intercropping systems, helping to maintain soil health, reduce pests and diseases, and promote biodiversity.

Agroclimatology plays a vital role in the cultivation and production of creeper vegetables in India. Creeper vegetables, such as cucumber, bitter gourd, and bottle gourd, are an essential part of Indian cuisine and are grown extensively throughout the country. With its diverse climate and geographical conditions, India offers a suitable environment for the growth of a wide range of creeper
vegetables.
In terms of area and production, creeper vegetables account for a significant share of the total vegetable production in India. According to the Ministry of Agriculture and Farmers Welfare, Government of India, the area under creeper vegetable cultivation has been increasing steadily over the years, with a corresponding rise in production. This trend is attributed to the growing
demand for these vegetables, both domestically and internationally.
Creeper vegetables are not only an important source of nutrition but also contribute significantly to the country’s economy. They are a vital source of income for many small and marginal farmers, who cultivate these crops on a large scale. Moreover, the export of creeper vegetables earns significant foreign exchange for the country.
The importance of creeper vegetables in India can be gauged from the fact that they are an integral part of various traditional and cultural festivals. For instance, bitter gourd is considered a auspicious vegetable in many Indian communities and is often prepared during special occasions.

Beans play a vital role in India’s agricultural sector and food security. They are an essential source of protein, fiber, and other essential nutrients, making them a crucial component of the Indian diet. Beans are also a significant source of income for many farmers in India, particularly small and marginal farmers. The country’s bean production also contributes significantly to the national
economy, with India being one of the largest producers of beans in the world. In addition to their nutritional and economic importance, beans also play a crucial role in maintaining soil fertility and promoting sustainable agriculture practices in India. Beans are legumes, which mean they have the ability to fix nitrogen in the soil, reducing the need for synthetic fertilizers and promoting
soil health. India is a significant producer of various types of beans, which are widely used as vegetables in the country. These beans are not only a good source of protein, fiber, and other essential nutrients but also play a crucial role in the country’s agricultural sector.
Overall, beans are a vital crop in India, and their production and consumption play a significant role in the country’s food security, agricultural sector, and national economy.
They are grown in various parts of the country, with major production areas including Punjab, Haryana, and Uttar Pradesh. According to the Indian Ministry of Agriculture and Farmers Welfare, the country produced over 1.4 million metric tons of kidney beans in 2020-21. Kidney beans are an essential ingredient in many popular Indian dishes, such as rajma masala, and are also used in salads, soups, and stews.

Leafy greens, comprising a diverse array of crops such as spinach, lettuce, kale, collard greens, and mustard greens, among others, are a vital component of global vegetable production. With their high nutritional value, versatility in culinary uses, and ease of cultivation, leafy greens (Fig. 6.1) have become an integral part of diets worldwide.
• In India, leafy greens are grown across various agroclimatic zones, with major producing states including Maharashtra, Gujarat, Uttar Pradesh, and Tamil Nadu. The estimated area under leafy greens cultivation in India ranges from 0.7 to 0.8 million hectares, accounting for a significant share of the country’s total vegetable production. The annual production of leafy greens in India stands at approximately 5-6 million tonnes, with a considerable portion being consumed domestically.
• Beyond their culinary and nutritional significance, leafy greens also hold substantial economic importance, particularly for small and marginal farmers who constitute the backbone of India’s agricultural sector. The cultivation of leafy greens provides these farmers with a vital source of income, enabling them to support their livelihoods and contribute to local economic development.
 

Root-type vegetables are a diverse group of underground plant structures that are edible and serve as a vital source of nutrition for humans. These vegetables can be further categorized into two main types: true roots, such as carrots, beets, turnips, and radishes, which are modified taproots that store food; and tuberous roots, such as Potatoes, sweet potatoes and yams, which are modified
stems that store food. A root is a compact, often enlarged storage organ with hairy stems that develops from root tissue. So a tuber is a root crop, but a plant can be a root and not a tuber. Carrots and cassava are root vegetable crops. Potatoes, sweet potatoes and yams, on the other hand, are edible tuber crops (Fig. 5.1).
The primary difference in propagation lies in the plant part used: tubers, like potatoes, are propagated from modified stems or roots with “eyes” (nodes) that sprout, while root crops, like carrots, are propagated from seeds or root cuttings, as their roots don’t have these propagation points. Another major difference is that tubers will have long shelf life compared to roots.
• Tubers
• Origin: Tubers are modified stems or roots that store nutrients.
• Propagation: They can be propagated vegetatively by using pieces of the tuber that contain “eyes” (nodes) which sprout and grow into new plants.
• Examples: Potatoes, sweet potatoes, and cassava.

1. Introduction

Zinc is an important micro-nutrient for human population and its deficiency can cause major diseases, mortality and thus becomes a major challenge in public health care systems of the developing nations. It was estimated that a major part of population is affected with deficiency of zinc (Combs, 2001) which causes lower growth, abnormal or decrease in infant birth, lowers the energy of body, decreases the power to lower the deadly infections, increase the mortality rate in children etc. (Gibson 2012; Krebs et al., 2014). Zinc also catalyzes various enzymes such as RNA polymerase, superoxidase dismutase and helps in working efficiency of cellular proteins. 

Application of manures and adoption of proper cultivation practices have profound influence in yield and quality of tubers. There crops also need macro nutrients (N,P,K which are primary nutrients and Ca, Mg and S which are secondary nutrients) and micro nutrients (Fe, Mn, Zn, Cu, Mo, B) for their maximum yield potenial.

the package of practices vary with crop and again with soil, climate, edaphic and topographic factors, especially with reference to tropical tuber and root crops. The yield can be maximized through good agronomical practice and by adoption of proven recommended production technologies. Soils, climate, topographic and edaphic factors suitable for each crop alongwith salient cultivation practices and production technologies are enunciated for important tuber and root crops in various parts of India.

Selection is an important activity in all the breeding programmes. Different types of selections i.e. mass selection, progeny selection and cyclic selection etc. are used depending on the mode of pollination of the crop species, the predominant gene action and the breeding objectives. The efficiency of selection largely depends on the extent of genetic variability present in the population and the heritability of the character. Selection is more effective for character with high heritability than those having low heritability. Breeding for higher or better yield is the breeding objective for most of the breeding programmes, but it has low heritability. Yield is considered to be complex or super character which is influenced by many components or contributing traits both in positive and in negative directions. Due to the fact that it has low heritability, selection is done for the yield contributing traits so that yield may be increased through selection. Prior to selection for yield contributing traits, it is always beneficial to study the extent and type of relationship between the character and yield. There are different procedures which aid in the isolation of superior yielding genotypes from genetically variable populations by providing information on indirect selection for yield. These methods are discussed in the following content.