eChapters

1. INTRODUCTION

Quality, i.e. the degree of excellence or superiority, is a combination of attributes, properties, or characteristics that each commodity value in of human food. The relative importance of each quality component depends upon the commodity and its intended use (e.g. fresh or processed) and varies among producers, handlers, and consumers. Appearance of quality, firmness and shelf-life are important from the point of view of wholesale and retail marketers. Consumers judge quality of fresh fruits and vegetables on the basis of appearance and firmness, flavour (eating) quality of the product. Consumers are also concerned about the nutritional quality and safety of fresh fruit and vegetables.

Maturity Standards and Harvesting Indices

Harvesting : Harvesting is detaching a commodity from the point of its origin. This point of origin may be an above ground plant part i.e. shoot eg. apple, tomato etc. or an underground plant part eg potato, carrot, etc. or in water i.e. harvesting of fish from a pond (not to be covered in this book).

19.1. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. It is also defined as the combination of attributes or characteristics of the products that has significance in determining the degree of acceptability of the product to a user. Industry defines quality as the measure of purity, strength, flavour, colour, size, maturity, workmanship or any other distinctive attribute or characteristics of the product. According to USDA Marketing Workshop Report (1951) quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user . The quality standards of fresh and processed fruit or vegetable products vary with their intended use. For marketing purposes; size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind. While for processing, physico chemical attributes of raw material such as presence of soluble solids, development of uniform colour, flavour, juiciness, uniform maturity, tenderness in some vegetables etc are taken into consideration. During processing of fruit or vegetables into value added products; colour, flavour and texture etc also become important.

1. Introduction
Quality makes the product what it is. Quality is how well a product or service satisfies the needs of the customer. Quality can be interpreted in several ways, such as conformance to the standards, meeting customers’ preference/ satisfaction for desired quality attributes, degree of excellence, zero-defect products, etc.
The quality standard of fresh and processed fruit or vegetable products varies with their intended use. For marketing purposes, size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind in fresh and processed fruit or vegetable products. During the processing of fruit or vegetables into value-added products, colour, flavour and texture also become important. Therefore, Quality is defined in different ways, such as:
i. Quality is a measure of the degree of excellence or degree of acceptability by the consumer.
ii. Quality is also defined as the combination of attributes or characteristics of the products that have significance in determining the degree of acceptability of the product to a user.

Quality is an increasingly important factor in the production and marketing of biological products. Consumers are becoming more discerning as their affluence increases, and would be suppliers of products must meet these demands if they are to maintain or increase market share. The term ‘quality’ is one of the most defined terms in use in food industry today. Quality may be defined as ‘The totality of features and characteristics of a product that bear on its ability to satisfy a given need’. The first part, ‘The totality of features and characteristics of a product…..’ concerns objective factors related to the product.  The second part, ‘…… to satisfy a given need’, concerns subjective factors related to user or consumer of goods.

Seed processing efficiency (SPE) is one of the important aspects in seed processing. It will help in measuring the potential of the shop floor. It will also help in standardizing activities like screens and improving physical purity. SPE will help to improve the quality of the seed material and help in utilizing the machines at their optimum capacity. SPE may vary among the different crops and even among the varieties.
 
To quantify SPE we need to pass the test sample (bulk-pre processed) through the target machine and enter the details in a processing register. To quantify the SPE we need to follow these mathematical formulae:

Measurement of soil moisture content is one of the most routinely done soil characterization. Knowledge of soil moisture is essential to understand the behaviour of soil as a medium for plant growth. Moreover, this property of soil helps to explain other processes of the soil. There are several methods of measuring soil moisture content. The most commonly used methods are gravimetric and tensiometric. The standard gravimetric method is quite accurate and simple but is time consuming, cumbersome and destructive. Furthermore, due to relatively small soil samples that are taken, the variability between replicates is great.

Gravimetric Method

Equipment needed includes zip-loc bags or aluminium moisture boxes and soil sampling tools. Soil sampling tools can be a soil probe, auger, or even a post-hole digger. Balance and scale should have accuracy to 0.1 gram. Proper sampling of the field is important to obtain a representative sample of the field. The soil sample should be collected from the field at a minimum of three locations, and the sample locations chosen should represent the entire field. Sampling from the small areas of low spots or ridges that do not represent majority of the field should be avoided. If there are major changes in soil classification within a field, the field should be divided into sub-sites and samples are collected accordingly.

‘Prunes’ is prepared from peach.

Arrak” liquor is prepared from date palm.

Hayane” variety of date palm suitable for juice making.

Kanji” is made from black carrot.

Kirschwascer” a wine prepared from cherry.

Shahi” variety of Litchi suitable for canning.

Introduction

Germination is the process by which a plant grows from a seed or similar structure. The most common example of germination is the sprouting of a seedling from a seed of an angiosperm or gymnosperm. Quick germination is the various ways and means that really fastened the process of seed germination. Through various ways, it is possible to reduce the time of germination by 70 per cent and also the success rate of germination can also be doubled.

The relative long periods of time required for completion of germination tests delays the seed marketing. This necessiated the development of rapid methods for estimating the germination capacity of seeds. This test was developed by Lakon (1942) in Germany.

1. PRINCIPLE

It is a biochemical test, in which living cells are made visible by reduction of an indicator dye. The indicator used is 2,3,5 triphenyl tetrazolium chloride. Within the seed tissues, it interferes with the reduction processes of living cells and accepts hydrogen from the hydrogenases. By hydrogenation of the 2,3,5-triphenyl tetrazolium chloride, a red stable and non diffusible substance, triphenyl formazan is produced in living cells (Fig. 1).

In managing diseases of horticultural crops, pathogen quiescence has important implications in timing prophylaxis, the reduction of stresses that trigger the transition of quiescence to aggression, and managing the harvested crop to prolong quiescence to the point at which yield is no longer affected. The inception of pathogen quiescence and its maintenance on or within the host implies a dynamic equilibrium between the host, the pathogen, and their environment. Physiological and physical changes in the host, its environment, or both trigger changes in that equilibrium to permit the pathogen to resume aggression. Microorganisms lying on the host surface constitute a potential latent infection; as can be the case with Botrytis cinerea conidia that are prevented from germinating by antagonistic bacteria on leaf surfaces (William R. Jarvis, 1994). Quiescence can run the spectrum from the ungerminated spore; through symptomless, internal infections; to visible but nonexpanding lesions, such as ghost spot of tomato (Lycopersicon esculentum) to expanding lesions on banana caused by Colletotrichum musae and on avocado caused by C. gloeosporioides.

A latent, dormant, or quiescent parasitic relationship is a condition in which the pathogen spends long periods during the host’s life in a quiescent stage until, under specific circumstances, it becomes active. Epidemiologists might refer to the period from spore landing until the parasitized tissue produces new spores as a latent period (Dov Prusky, 1996). The term “period of quiescence” is referred to differentiate the quiescent parasitic relationship from the latent period. Quiescence can be enforced during the various processes of fungal attack, which suggests that quiescence may or could occur during any of the processes leading from fungal germination to colonization.

Radish (Raphanus sativus, 2n = 2x = 18, family Brassicaceae or Cruciferae, cross-pollinated crop) is grown over 7 million ha where China, Japan, India, South Korea, and Europe are the main producers. As per Horticulture Statistics at a Glance-2018 (MAFW, 2018), India produced 30 lakh tons of radish from 2 lakh ha acreage with a productivity of 15 tons/ha during 2017-18. Ten top ranking states in India with acreage within the range of 6000 to 33000 ha are West Bengal, Haryana, Bihar, Assam, Punjab, Chhattisgarh, Orissa, Madhya Pradesh, Uttar Pradesh and Karnataka.

Today, radishes are grown throughout the world. Different local people prefer to use various parts of the radish plants including roots, leaves, sprouts, seed pods and oil from seeds as their food according to their own custom. Radishes are low in calories and high in vitamin C, folate, and potassium. Radishes contain sulfurous compounds, such as sulforaphane, which have anti-cancer properties, and are expectorant.

It is a popular root crop having wide range of adaptability, grown in Japan, Koria, China, India and other European countries.

Origin and Distribution: Radish was an important food in Egypt during 2000 BC.  Fossils study reveals that, it was a food as early as 2700 BC. It spread to China during 500 BC and to Japan during AD 700. Variability of crop decreased from Europe to China and from there to Japan. Recent study indicates that East of Mediterranean, most probably China was the centre of origin of the crop. This means Europe and Asia are the probable places of origin.

Radish, Raphanus sativus under the family Brassicaceae (Crucifereae) having chromosome No. 2n = 2x =18 is grown mainly for fleshy taproot roots in both tropical and temperate regions throughout the world. Radish is cultivated widely in China, Japan, Korea, India, Europe and USA. It is grown throughout India and particularly in Uttar Pradesh, Bihar, West Bengal, Punjab, Haryana, Himachal Pradesh, Assam and Gujarat. Radish is mostly eaten raw as a crunchy salad or cooked as a vegetable. Leaves are sometimes eaten as cooked leafy vegetable. Immature pods of rat-tail radish, Raphanus sativus var. caudatus are also consumed as vegetable. Origin, domestication and adaptation in India
Radish, one of the ancient root vegetables was domesticated in Asia prior to Roman times. Varieties of radish are now broadly distributed around the world, but almost no archeological records are available to help determine their early history and domestication. However, different research works have tentatively located the origin of Raphanus sativus in Southeast Asia, as this is the only region where truly wild forms have been discovered. This radish might be Oriental or Asiatic radish. India, Central China, and Central Asia appear to have been the secondary centers where different forms were developed. However, according to other hypothesis, eastern Mediterranean region, China and middle Asia are considered to be the origin of radish where wild radish still exists.

ABSTRACT

Random Amplified Polymorphic DNA (RAPD) markers were used for characterizing eleven banana (Musa AAB Plantain Subgroup) clones using 40 decamer primers. Eight primers, which produced reproducible banding patterns, yielded 42 scorable bands with an average of 5.25 bands per primer. The amplification products ranged in size from 400 to 1500 bp. A genetic similarity matrix was constructed using the Jaccard’s coeffecient method. The pair wise similarity coefficient values varied between 0.3333 and 0.9355. Based on the coefficients, distances between the clones were computed using SYSTAT software package. In the dendrogram constructed by the nearest neighbour (single- link) method (Krzanowski, 1988), all the eleven clones were found grouped under five clusters. Attu Nendran, Changanasseri Nendran, Changazhikodan, Kaliethan, Koonoor Ethan and Quintal Banana formed the largest cluster. Manjeri Nendran and Myndoli formed the second cluster. Padalamurian, Mysore Ethan and Zanzibar formed three separate clusters. Zanzibar, belonging to the Horn Plantain group was different from the rest of the clones.

ABSTRACT

There is a wide variability in the contents of the commercially important chemical compounds of Centella asiatica depending on the source / ecotypes of the plants. The glycosides found in Indian plants include brahmoside, brahminoside and minor amounts of asiaticosides, which vary in their relative contents depending on the ecotypes. An RAPD marker assisted study of ten accessions, collected from different geographical locations of Kerala, viz., Parumala (Kottayam District), Kottayam, Nagercoil (Tamil Nadu), Mananthala (Trivandrum District), Vlathankara (Trivandrum District), Ponmudi (High range, Trivandrum District), Angamaly (Ernakulam District), Punalur (Kollam district), Pattambi (Palakkad district) and Calicut, was conducted. DNA amplification of the accessions was studied using forty decamer primers, involving 43 cycles. Based on the number of bands four primers were selected. The least similarity coefficient of 0.71 was shown by the Pattambi accession, which formed a separate cluster with all other accessions. Nagercoil and Ponmudi accessions showed the highest similarity coefficient of 0.88 with each other.

ABSTRACT

Hevea brasiliensis, the main source of natural rubber (NR) is commercially propagated through bud grafting. Since Hevea brasiliensis is a cross-pollinated tree crop, seeds obtained are highly heterozygous. Earlier, plantations were grown from unselected seeds. But due to poor yield and large tree - to - tree variations in growth and yield in such plantations bud grafting with desirable scions is now being adopted for commercial planting. Genetically divergent seedlings grown from open pollinated seeds are used as rootstocks to graft the selected scion. This can lead to stock - scion interactions. Large intraclonal variations observed in growth and yield of bud-grafted clones of Hevea are attributed to the genetic heterogeneity of the rootstocks. Monoclonal and polyclonal seed gardens have been established in Kanya Kumari District of Tamil Nadu for producing good quality seeds. Monoclonal seeds are collected from monoclonal seed gardens where only one clone is planted in a vast area without any interference from pollen grains from other clones. Polyclonal seeds are collected from polyclonal seed gardens where different clones are planted in an area and the seeds are fertilized by pollen grains from all the clones. It is a general assumption that seedlings grown from polyclonal seeds may be highly heterogeneous than seedlings grown from monoclonal seeds. The objective of the present study was to find out the reliability of this assumption by studying the genetic variations existing among the polyclonal and monoclonal seedlings. RAPD analysis of leaf samples from polyclonal and monoclonal viz, RRIM 600, PB 86 and GT1 seedlings was carried out using ten Operon arbitrary decamer primers that gave informative amplification. The results showed that there was no significant difference in the genetic variations among the polyclonal and monoclonal seedlings.

ABSTRACT

Vitex trifolia L. (Verbenaceae) is known for its medicinal property. Micropropagation can provide the opportunity to obtain large number of homogeneous plants and there are no reports of micropropagation of this species. So, we attempted for in vitro propagation of this species and described here is an efficient and rapid propagation method. This report describes in vitro shoot induction and plant regeneration from mature nodal explants of Vitex trifolia L. on Murashige and Skoog (MS) medium fortified with benzylaminopurine (BAP), kinetin (KN), thidiazuron (TDZ), adenine (ADE), and 2- isopentenyladenine (2 - iP) (0.25-10.0 mM). Multiple shoots differentiated directly without callus mediation within 3 weeks when explants were cultured on medium supplemented with cytokinins. The maximum number of shoots (9 shoots per explant) was developed on a medium supplemented with 5.0 mM BAP. Shoot cultures was established repeatedly by subculturing the original nodal explant on the same medium. Rooting of shoots was achieved on half strength MS medium supplemented with 0.5 mM naphthaleneacetic acid (NAA). Rooted plantlets were transferred to pots containing autoclaved soil and vermiculite mixture (1:1) that showed 90 % survival when transferred to outdoor. Plants transferred to the field have established themselves in the soil and are growing well. The regenerated plants did not show any immediately detectable phenotypic variation. Natural stands of Vitex trifolia are fast disappearing in India because of indiscriminate collection and over exploitation and the outlined procedure here offers a potential system for mass multiplication and conservation of Vitex trifolia.

ABSTRACT

Cashew (Anacardium occidentale L.) is a woody perennial tree of tropical origin, belonging to the family Anacardiaceae. Cashew is being cultivated in India, Brazil and Africa countries. It is well known for its food, noon food and medicinal uses. In vitro clonal propagation of cashew, which is an effective method for large-scale multiplication of trees was tried. Cashew plantlets were regenerated from shoot explants of 2 - 3 year old grafted plants, which were regularly sprayed with Bavistin 0.1 per cent at weekly intervals. Among the different surface sterilization treatments used, a combination of antioxidants, antibiotics and fungicides was found to be better with around 40 per cent contamination free cultures being obtained. MS medium containing full strength macro elements supplemented with Kinetin (5 mg l ^-1), NAA (1mg l ^-1) and Brassinolide (0.1 mg l ^-1) was found to be best for shoot proliferation and shoot elongation. Micro shoots were rooted in vitro at a frequency of 70-80 per cent, when cultured for 4-8 days in quarter MS liquid medium supplemented with IBA (1mg l ^-1) after the pulse treatment with IBA (100 mg l ^-1) for two minutes. The average number of roots ranged from 3 - 5 with an average length of 1.63 cm. About 50 - 60 per cent of the rooted microshoots survived wearing and produced healthy and vigorously growing plants, which were transferred successfully to the field.

ABSTRACT

Asimple and reproducible protocol for the rapid propagation of Clitoria ternatea L., a flowering garden climber cum medicinally valuable plant, was developed through somatic embryogenesis from the leaf explants. Leaf segments excised from 12 days old in vitro raised seedlings on inducing callus by culturing in MS medium containing 0.5 mgl^-12,4-D and 0.1 mgl^-1 BAP and sub-culturing callus to MS medium containing 1.0 mgl^-1 BAP, produced numerous somatic embryos within 30 - 45 days. Embryos germinated and produced plantlets with intact shoot and root when they were cultured in MS medium devoid of any hormones and supplemented with GA₂ 0.1 to 1.0 mgl^-1. Elongation of plantlets could be achieved on hormone free MS medium in about 3 - 4 weeks. Well- grown plants on transfer to pots, containing sterile sand, in a growth chamber and three weeks later to the greenhouse gave about 40 per cent survival.