eChapters

1. SEED LOT

A specified quantity of seed which is physically identifiable to the types of which certificates may be issued.

2. SAMPLING

A sample is obtained from the seed lot by taking small portions at random from different position of the lot and combining them. It is a representative sample of a seed lot.

The objective of sampling is to obtain a representative sample of a size suitable for test. When the sample originates from seed lot, it is expected that the results reflect the average quality of the seed lot.

Nutraceuticals have received considerable attention for their expected safety and potential nutritive and therapeutic effects. These were used as alternatives to modern medicines that promote quality of health, increase the nutritional value of the diet, and prolong life expectancy. Major constituents of nutraceuticals are herbals; various nutrients and dietary supplements are involved in preventing different diseases and minimizing the pathophysiology of the disease too. It also acts as an immune boosting, natural antioxidant, anticancer, anti-inflammatory, anti-diabetic, cardioprotective, and organ protective agent, in addition to different health-promoting effects. Ultimately, they ensure a better quality of life.

The scope of the nutraceutical field is plenty both in terms of type and the varieties. The nutraceuticals industry in India is one of the rapidly growing markets. Higher- and upper-middle-class consumers are perceiving nutra ceuticals as alternatives to prescribed drugs and exclusively for their beneficial properties without any side effects. Consumers are showing sharp interest in nutraceuticals for boosting energy and improving their physical endurance and mental alertness. Nutraceutical industries are focusing on developing new products with innovative formulations and using proper advertisements to choose the right products. Nutraceuticals have significant promise in the promotion of human health and the prevention of disease. They are widely accepted by all age groups due to their safety, higher quality, purity, efficacy, health-promoting, and disease-curing activities. The newest trend is moving toward nutraceuticals, leading to a new era of medicine and health. It is still in its infancy in India. But in this hype era, we must say, “let food be your medicine” and “proper nutraceuticals daily can keep the medicine away.”

1. Isolation distance

According to Harrington’s rule (1960) of thumb, for storage, every one percent increase in moisture content, the seed life is halved far seeds of moisture content of 5-14%. For temperature, every 5^ºC rise in temperature, between 0-50 ^ºC, the seed life is halved.

Weight loss: For grains or grain legumes in the tropics, a weight loss in the range of 10-30 per cent might be expected over a full storage season.

Loss in quality/market value: Infested seed is contaminated with insect debris and has increased dust content. Seeds are holed and often discoloured, which fetch low price.

Promotion of mould development: In humid conditions, without adequate ventilation, mould development and caking can spread rapidly, causing severe losses.

Reduced germination in seed material: Damage to the embryo of the seed will usually prevent germination. Some storage pests prefer to attack the embryo.

1. SEED STORAGE

Is the maintenance of high seed germination and vigour from harvest until planting.

1.1. Importance of seed storage

Seed storage is important to get adequate plant stands in addition to healthy and vigorous plants.

Seed storage is the preservation of viable seeds from the time of collection to the required for sowing. It is important to get adequate plant stands in addition to healthy and vigourous plants.

Objectives

i. To maintain initial seed quality during storage period by providing suitable conditions.

ii. To maintain the seed germination and emergence rates.

iii. To prevent the seed deterioration during storage period.

iv. To maintain the physical and physiological seed qualities.

Seed Storage is an essential process during seed production programme. The purpose of seed storage is to preserve planting stocks from one season to next planting season.Seed storage starts from the moment of maturity and extend until its planting, in the next season.
 
PRINCIPLES OF STORAGE

Primary aim of storage is simply to prevent deterioration of quality seed. The management objective during the storage period is to reduce metabolic activity to such a low level that the seed is sufficiently stable with minimal deterioration. The two alternative methods are

1.    Reduction of moisture content to a safe level and cooling of the seed.
2.    Modification of atmospheric condition of the storage system.

Types of storage

There are four types

1.    Storage of commercial,  truthfully labelled and certified seed.

2.    Storage of carry-over seeds.

3.    Storage of foundation seed stocks and enforcement seed samples.

4.    Storage for germplasm seeds.

Seeds are having a ability to survive and start a new generation when favourable conditions exists. However like other form of life, they cannot retain their viability indefinitely and eventually deteriorate and die. To retain viability and germination it is important to store in proper storage. Seeds harvested may not be useful for packaging thus making it to store for a period till the need for packaging arises. Seed storage is very critical task of seed processing. The success of seed processing is determined by safe storage. Since the vegetable seeds are expensive need modern techniques of storage. Based on the seed shelf life or longevity vegetable seeds can be divided into two types:

Orthodox Seeds : Orthodox seeds are long-lived seeds. They can be successfully dried to moisture contents as low as 5% without injury and are able to tolerate freezing temperatures. Most orthodox seeds come from annual temperate species adapted to open fields. At physiological maturity they contain moisture content of 30 – 50%. Most of the vegetable seeds are orthodox and hence there is lot of scope for proper storage. E.g-Tomato (6.0 %) and Peppers (7.0%

Recalcitrant Seeds : They are short - lived seeds, which cannot be dried to moisture contents below 30% without injury and are unable to tolerate freezing. They are difficult to ore successfully because of their high moisture content encourages microbial contamination and results in more rapid seed deterioration. Storage of these seeds at subzero temperatures causes the formation of ice crystals, which disrupts cell membranes and causes freezing injury. These seeds are from perennial trees in the mois t tropics such as coconut, coffee, cacao, citrus etc. These seeds mature and exists in their fruits and are covered with fleshy or juicy ariloid layers and impermeable testa. At physiological maturity they contain more moisture content (50- 70%) than orthodox seeds, even though their embryos are only about 15 % of the size of an orthodox seed embryo. In general recalcitrant seeds never go into dormancy but instead continue their development and progress towards germination.

Seed

A true seed is defined as a fertilized mature ovule consisting of embryo, stored material and protective coats. The seed strictly speaking is an embryo a living organism embedded in the supporting or the food storage tissue.

A seed stands for any of the following used for sowing or planting.

1.    Seeds of food crops including edible oil seeds and seeds of fruit and vegetables.
2.    Cotton seeds
3.    Seeds of cattle fodder
4.    Jute seeds
5.    Seedlings, tubers, bulbs, rhizomes, roots, cuttings, all type of grafts and other vegetative propagated material for food crops or cattle fodder.

Supply chain management or inventory management is an important and integral component of commercial breeding activities practiced by the private seed companies whose over all research efforts in terms of developing and commercially releasing high performing cultivars and hybrids get defeated unless the product reaches to the end users that is the farmers. Seed supply chain management is the lifeline of any seed business especially when the seed business is under private sector seed companies whose delivery is very strong. Supply chain management refers to the process of ordering, storing, and using a company’s inventory. These include the management of raw materials, components, and finished products, as well as warehousing and processing such items and ultimately delivering virtually to the door steps of the farmers through a strong network of dealers/distributors/retailers. For companies with complex supply chains and manufacturing processes, balancing the risks of inventory gluts and shortages is especially difficult and challenging.

The process of supply chain management is a continuously evolving process, ready to deal with the unforeseen situations like the situation created by COVID-19 pandemic forcing countries after countries going for lockdown and bringing the entire business operations including material and men transport to a grinding halt. However, even under these extremely challenging situations, governments fully know that agricultural activities cannot be fully stopped to avoid resurgence of hunger and malnutrition. However, despite the window allowed by the government for running the supply of essential goods including agricultural inputs like seed, agrochemicals and farm machines, manufacturing the agro-inputs including seeds and their distribution to the endpoints has become extremely difficult. A few companies have devised new processes to run seed supply using completely new and innovative approaches, for example, In Bayer’s first-ever e-commerce collaboration in India, the company has tied up with Agro-Star, a Pune-based e-commerce firm in the agricultural segment. Under the partnership, farmers can order Bayer’s seeds and crop protection products for their entire crop lifecycle and receive agronomic advisory through Agro-Stars digital agri-tech platform. Home delivery of agri-inputs can currently be availed by farmers in North, West and Central India, with plans to extend the scope to other geographies in the future. In the current COVID-19 lockdown situation, the partnership has enabled last mile delivery of products for multiple crops directly to farmer’s doorsteps.

Seed Testing
1.    Sampling

2.    Determination of Heterogeneity

3.    Purity analysis

4.    Germination Test

5.    Seedling Evaluation

6.    Quick Test for Viability

7.    Estimation of Seed Moisture

8.    Grow Out Test

9.    Seed Health Test

Objective: To study the seed treatment methods and equipments A key factor in achieving the few potential of good quality seed is the blanket treatment of the seeds with agrochemical in order to control or prevent diseases in the  crops.

Seed is the basic input of agriculture, and its importance has now well recognized in the food supply chain since the warranty for production could be succeeded only based on the availability of seed. Seed becomes the quality seed, on possession of specific characters, viz. germination, vigour, purity and health status, hence, seed production becomes the specified scientific technology with the foreseeing of quality and storability in addition to productivity. From time immemorial, seeds are being imposed with some management techniques for improvement in seed quality and thereby the productivity. These seed management techniques are known as seed treatments. Generally, seed treatment is the application of biological, chemical agents and physical techniques to the seed in order to obtain the benefit of plant protection, production and invigouration. Basically, seed treatments are intended to improve the quality of seeds since nothing can substitute poor quality seed, no matter how lavishly other inputs are spent on the crop established from such seeds. Even for a resource poor farmer, high quality seed would be a great advantage because it ensures the best utility of all inputs in addition to the augmentation of yield increase by 15 to 20%.

In general, seed treatment could broadly be classified into physical and physiological treatment. Physical treatment is external seed treatment, while physiological is impregnation of needy substances into the seed through various seed management techniques. Some of the germination augmenting physiological seed treatments are seed fortification, seed hardening, seed priming, dry permeation and pre-germination. In addition, some of the seeds are imposed with special treatments such as dormancy breaking treatment such as soaking in water, mechanical scarification, acid scarification, bio-scarification, scorching, warm stratification, cold stratification and electrical seed treatment. The common physical seed treatments are seed pelleting, seed coating/film coating and seed colouring. Seeds are also imposed with some other physical treatments that can act on the physiology of the seed such as irradiation, magnetic and electrical seed treatment. In addition, irrespective of seed, seeds are given with protective treatment when they are taken for storage, which are commonly termed as pesticide seed treatment. Seeds are also given with corrective treatment at their initiation reduction with seed quality characters during storage, which are termed as mid storage correction.

1. SEED TREATMENT

It refers to the application of pesticides (fungicides, insecticides or a combination of both) to seeds before sowing and/or storage in order to protect the seeds and young seedlings from soil borne pathogens and/or storage pathogens and insects. Seeds are thoroughly mixed with required quantities of chemicals namely bavistin (carbendezim) @ 2-4 g/kg of seeds.

Seed treatment is one of the important activities of the shop floor. The seed should be treated with various insecticides, fungicides and additives to protect it up to establishment. Some timesenhance quality parameters like vigour which is critical for better establishment. Since vegetable seeds are of very high value and low volume, investment on seed treatment will yield better not only for the seed seller but to the grower also. More number of plants in a field brings moreincome per unit area. Hence the farmer or grower who buys seed is ready to bear the cost of seed treatment making it an important aspect of seed processing.

Seed treatment refers to the application of fungicide, insecticide or both to the seeds to disinfect(deep seated) and disinfest (over seed coat) them from seed borne or soil borne pathogenic organisms and storage insects. It also refers treating seed for enhancing quality by subjecting the seed to solar energy exposure or immersion in hot water or dry heat treatment or priming also.

Seed viability tests

Only two methods, the Topographical tetrazolium (TZ) test and the embryo excision test were previously accepted by the International Seed Testing Association as official methods for some species of seeds. ISTA has recently accepted the X-ray method as a valid alternative to the cutting test for the detection of empty and insect-damaged seeds. The following tests can be applied depending on the circumstances.

Seed Viability Test

Germination test for a seed lot is a pre-requisite to know its potential of emergence under field condition. However, this test requires few days to weeks and a month in some cases. Tetrazolium test gives quick information on seed viability or germinability. It makes possible to distinguish red coloured living parts of seeds from the colourless dead ones. Staining of seeds determines  viability.  Completely  stained  seeds  are  viable  while,  partially and comparatively unstained seeds are non-viable. This test is not valid as compared to seed germination test.

Introduction

Seed vigour is an important quality parameter, which needs to be assessed to supplement germination and viability tests to gain insight into the performance of a seed lot in the field or in storage. Several definitions have been offered to explain seed vigour. Isley (1957) defined vigour as “the sum total of all seed attributes, which favour stand establishment under unfavourable field conditions”. While, AOSA (2005) defined seed vigour as the sum total of all those properties in seed, which upon planting, result in rapid and uniform production of healthy seedlings under a wide range of environments, including both favourable and stress conditions and ISTA (1985) stated that seed vigour is the sum total of those properties of the seed, which determine the potential level of performance and activity of a non-dormant seed or seed lot during germination and seedling emergence.

1. DEFINITION

Sum of those properties of seed, which determine the potential level of activity and performance of the seed or seed lot during germination and seedling emergence. i.e., rapid and uniform production of healthy seedling and stand establishment under a wide range of field conditions is considered as seed vigour.

In a vigour test, the seed is introduced to a stressful environment unfavorable to seedling development. This environment can be cool, cold or warm, or a combination of either high humidity and high temperatures, or heavy moisture at low temperatures. A vigor test cannot replace a germination test, but rather supplements it with more information about seed quality.