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Origin and history: Apple is a “King of temperate fruits” and it’s belonging to family roasaceae. Plants are deciduous in nature. The apple fruits are well known for its sweet, pomaceous fruits. Apples have been grown for thousands of years in Asia and Europe, and were brought to North America by European colonists. Apples have religious and mythological significance in many cultures, including Norse, Greek and European Christian traditions. Apple is the premier table fruit of the world. It is typical temperate fruit tree, about 40 percent of the world’s supply being produced in China. Major apple growing countries are U.S.A, Italy, west Garmany, France, Japan and China. In India a large number of apple variety were introduce in 1887 by Mr. Alexander Coutts in his orchard called hillock head in Mashobra near Shimla, Himachal Pradesh. It was also introduce in Bangalore and Nilgiri hills (Tamil Nnadu). But it was destroy by woolly aphid in 1897. In India apple is commercially cultivated in Himachal Pradesh, Uttar Pradesh, Uttarakhand, Jammu and Kashmir and Arunanchal Pradesh.

I am happy to note that the Indian Agricultural Research Institute, a premier national institute for agricultural research is celebrating the 100th year of its establishment. It is indeed quite fitting that JARI has chosen to celebrate this occasion by organizing a seminar on current scenario in temperate horticulture in Himachal Pradesh which is the second highest producer of temperate fruits in the country, I, therefore consider myself to be privileged to be a key speaker, and express my appreciation and gratitude to the organizers for giving me this honour and opportunity in this very important event.
 
The varied climatic and soil conditions in India provide enormous scope for cultivation of wide range of tropical, sub-tropical and temperate horticultural crops, The production of tropical and sub-tropical horticultural crops is confined to the plains, coastal and root hills of Indian mountain~ whereas, the temperate horticulture is monopoly of the hilly regions of the country. The production of temperate fruits in the country is spread over some fifteen states and union territories situated in the following regions.

Onion is one of the most important commercial vegetable crops grown all over India. Onions are bulbous, biennial, herbaceous and are typically grown as annual, which give off a distinctive and pungent odour when the tissues are crushed. Characteristic pungency and flavour in onion are due to a volatile sulphur compound, allyl-propyl disulphide. This organo sulphur compound has potential for anticarcinogenic secondary metabolites. Pungency varies with cultivar, growing condition, stage of maturity and storage conditions.

It is such a unique vegetable, used throughout the year in the form of salad or condiment or for cooking with other vegetables. Onions are used for flavour, aroma and taste in preparation of vegetables and used in the form of dehydrated, freezing, canning and pickling. Onion salt is prepared by dehydration and used in flavouring.

Purpose: This test is conducted to determine the boldness and soundness of the seeds. The weight of a given number of seeds of different varieties or kinds will vary. This variation is due to boldness and soundness of the seeds. The seeds will vary according to the soil fertility, variety and other environmental conditions. Higher the weight of a variety per thousand seeds, better is the variety for cultivation.

Materials and equipments: Scale, balance and seeds.

Introduction

Seeds are regarded as carriers of new technology and serve as basic catalytic input for enhancing the agricultural production. Hence the timely availability of quality seeds of the right variety in adequate quantity decides the strength of an agricultural economy. The intensive crop improvement programmes have resulted in the development of large number of hybrids and high yielding varieties in many important crops. Today, the Indian seed industry is the fifth largest seed market in the world, accounting for 4.4 per cent of global seed market. In terms of global trade, India is almost self-sufficient in flower, fruits and vegetables and field crops seeds (Anonymous, 2015). Economy of crops depends on good crop variety. Farmers choose these verities based on good quality traits and satisfactory yield. Thus, it is always essential to do fidelity check of all plant variety. The genetic purity is one of the most important aspects of quality control. Seed quality includes good germination, purity, seed health and vigour. With the increase in seed industry, there has been a refinement in the techniques used for testing genetic purity or genuineness of seed purity. Methods for testing genetic purity include different morphological, chemical, biochemical and molecular markers. Under PPV & FR Act, DUS (distinctiveness, uniformity, stability) testing procedure is performed with morphological descriptors. However, in agriculture, biotechnology has found applications in checking of true to type of clones and variety identification programs using molecular and chemical markers. Markers that reveal polymorphisms at the protein level are known as biochemical markers, while DNA markers reveal polymorphisms at the DNA level.

Introduction

Test of pollen viability (extent of pollen viability) is important in pollination experiments to ensure fertility/seed setting. It is also important in monitoring state of pollens during storage in ecological/ taxonomic studies as well as during research on pollen biochemistry, genetics, stigma interactions, and incompatibility studies. Pollen viability refers to the ability of pollen to accomplish post-pollination events to achieve fertilization. Pollen viability test has gained importance particularly in plant breeding and hybrid seed production experiments/ practices. In this chapter, you will be acquainted with the basics of pollen viability test. However, for more professional knowledge on the topic readers are suggested to refer specialized books.

Pollen Viability Test

It is important to know the extent of viability of pollens used in pollination experiment, in finding fertility of a plant of interest, to monitor state of pollen on storage, in ecological/taxonomic studies, as well as in research on biochemistry, genetics, stigma interaction, incompatibility assay, etc. of pollen. Several pollen viability tests have been developed time-to-time. It is recommended to initially use different tests for a given pollen type to find out the most appropriate method that provides reliable result on pollen viability. It is important to note that every procedure requires standardization of protocol according to the sample used. Following different tests are used for assessing pollen viability.

1. In-vitro Pollen Germination Test

It is a simple, speedy, and quantitative test for assessing pollen viability. In this method, a collection/sample of pollen is germinated in vitro and observed under microscope. The percentage of germinating pollens (producing pollen tube) after appropriate treatment and a given period of time is determined. The percentage provides an index of viability of the pollen sample.

Introduction

Seed is a living entity (mature ovule) consisting of a tiny plant in embryonic stage along with the stored food protected by an outer layer known as seed coat. Seed is an important biological input in agriculture, which is economically important for successful crop husbandry. Cotyledons serve as the source of nutrients (proteins, carbohydrates, and oils) that not only nurture the plant at the early developmental (seedling) stages of plant but also the vegetarian (heterotroph) organisms. Being one of the important inputs in agriculture, viability of seed needs to be assessed to know the germination percentage of seeds used to raise a crop. Since viability of seeds may deteriorate on storage depending on the storage conditions. Therefore, often it becomes important to test viability/germinability and vigour of seeds before being used for raising a crop or conducting an experiment. In this chapter, we briefly discuss about the different methods which can be used for testing the viability of seeds.

Seed Viability

Seed viability can be defined as the capability of the seed to germinate and produce a normal seedling within a specified period. In other words, seed viability indicates how many seeds in a lot are alive and could develop into plants under appropriate field conditions. It is very important that most of the stored seeds are capable of germinating when sown in a field. They must possess higher viability at the beginning of storage and maintain it over the storage. A seed with high initial viability survives longer in storage; however, the viability deteriorates slowly at first and then rapidly as the storage prolongs. It is important to know the duration of storage after which a considerable decline in viability occurs under given storage conditions so that to take necessary action to revive the seeds. Excessive deterioration in vianility of seeds may lead to loss of the seed/material/ germplasm.

The circumstances concerning the diffusion of  main cultivated citrus from their places of origin in Asia are studied here, showing that the citron (Citrus medica L.) was the only one known in Ancient times in Europe, while the lemon (C. limon [L.] Osbeck), lime (C. aurantiifolia [Christm.] Swingle), pomelo (C. maxima [Burm.] Merr.) and sour orange (C. × aurantium L.) were introduced to Europe by the Muslims via the Iberian Peninsula and Sicily, and that the grapefruit (C. × aurantium L. [C. paradisi Macfad.]), mandarin (C. reticulata Blanco) and sweet orange (C. × aurantium L.) arrived to the West between the fifteenth and nineteenth centuries as a result of the trade with the British and Portuguese colonies.

Mechanical damage in oil seeds like soya bean can be induced at any time during harvesting, handling, conditioning and sowing operations. There is relationship between moisture and mechanical damage. In general the low moisture containing seeds are more prone for mechanical damage than the high moisture containing seeds. In this experiment we look for this relationship by measuring seed quality parameters like germination and leakage (electrical conductivity).

The Seed Act 1966 was passed by the Indian Parliament on 29th December 1966 and it came into force from 2^nd October, 1969. In addition, Seed Regulation Order was also passed in December 1983. The sole objective of Seed Act and Order has been to ensure that the Indian farmers get good quality seeds of important crops. Important provisions and guidelines for the farmers are given below:

The Act is applicable for the seeds notified by the Central Government from time to time.

Seed quality is regulated at two stages viz. seed production and seed certification. For this purpose, seed testing laboratories and seed certification agencies are to be established in each state. Maharashtra has been the first state to implement this act. Now all the states have implemented this act.

Preliminary

1. Short title and extent

1. This Order may be called the Seeds (Control) Order, 1983
2. It extends to the whole of India
3. It shall come into force on the 30th December, 1983

An Act to provide for regulating the quality of certain seeds for sale, and for matters connected therewith. It enacted by Parliament in the Seventeenth Year of the Republic of India.

1. Short Title, Extent and Commencement

• This Act may be called the Seeds Act, 1966.
• It extends to the whole of India.
• It shall come into force on such date as the Central Government may, by notification in the Official Gazette, appoint, and different dates may be appointed for different provisions of this Act, and for different States or for different areas thereof.

A bill to provide for regulating the quality of seeds for sale, import and export and to facilitate production and supply of seeds of quality and for matters connected therewith or incidental thereto. Be it enacted by Parliament in the Fifty-Fifth Year of the Republic of India as follows

Part I - Preliminary

1. Short title: These rules may be called the Seeds Rules, 1968
2. Definitions: In these rules, unless the contest otherwise requires.

Agriculture is under increasing pressure to produce more food, feed, and biofuel from limited land resources. The current over-reliance on a few key staple crops causes a variety of agronomic, ecological, nutritional, and economic issues and is likely unsustainable in the long run.

Vegetables have a high commercial value in general and hence contribute significantly to household income. Significant research, breeding, and development initiatives are necessary for a number of promising crops to transform existing local landraces into competitive cultivars with broad adaptability and high commercial prospects. India has a good genetic wealth of domesticated crops, and 600 plant species represent the diversity in vegetable crops out of 20,000 angiosperm plants; however, only one-fourth of these are used as major vegetable crops, with the rest classified as minor, underutilized/neglected, rare vegetables, lesser-known vegetables, wild edible vegetables, and so on. Access to the genetic diversity of these selected crops is required for success

1. INTRODUCTION

Thermal processing means preservation by application of heat. It involves heating of foods in hermetically sealed containers for a specific time at a specific temperature to eliminate the microbial pathogens that endanger public health and that micro-organisms that deteriorate food during storage. The credit for invention of thermal processing (Canning) goes to a French Confectioner, Nichola Appert. The application of heat is an important method of preserving foods and is also a means of developing texture, flavour and colour in some foods.

Removal of seeds from dry fruits is known as threshing while that of wet fruits is known as extraction. Threshing involves beating or rubbing the plant material to detach the seed from its pod or fruit. The detached seed is then winnowed to remove chaff, straw and other light material from the seed.

Threshing / extraction methods

1. Mechanical threshing

• Various types of threshing machines with adjustable cylinder speeds are available for extraction of vegetable seeds.
• Care must be taken to avoid damage to the seed during mechanical threshing, by properly adjusting the speed of the beaters, the width of the gap between the beaters and the concave, the airflow and the sieve sizes.

15.1 Introduction

Plant breeding aims at developing genetically improved crop cultivars with economic benefits for small-scale and commercial farmers. Population growth, dwindling agricultural land and global climate change present increasing risk to crop production. Consequently, plant breeding aims to constantly develop crop cultivars with improved yield and quality and tolerant to drought, diseases and pests. Use of genetically improved crop cultivars and better management practices are among the best strategies to increase food production and meet a projected doubling of food demand in the next 40 years. Both the public and private plant breeding sectors face several constraints, including a shortage of professional plant breeders and breeding technicians, limited budget, inadequate facilities and lack of access to information. Successful plant breeding requires adequate infrastructural investment, in addition to the actual breeding activities. The benefits of plant breeding research and development can only be realized on a long-term basis because of the inherent nature of the crops and the eminent breeding procedures involved. Plant breeding involves two main activities, i.e., pre-breeding/ germplasm enhancement and cultivar development per se. These interdependent activities are the controlling factors that determine the pace at which cultivars are released timeously and consistently to farmers. The two phases, in turn, depend upon various factors, e.g., breeding goals, genetics and agronomy of the crop, breeder’s long-term objectives, availability of testing facilities and national cultivar-registration requirements. Despite these factors, there are certain clear steps and breeding procedures found in any conventional breeding programme. These include choice of parents, making crosses among chosen parents, and selection from recombined parents followed by extensive field testing of the candidate cultivars. Maintenance, multiplication and distribution of the seed are the ultimate stages of a breeding programme. Systematically outlined conventional breeding procedures, timeframes and estimated breeding generations are essential to objectively consider the ultimate timescales required to release new and improved cultivars of all crops. The information may assist plant breeding- students, educators, researchers, project advisors, administrators and funding agencies. Dear students, in this chapter we outline the timeframes of conventional breeding procedures of clonally (also called vegetatively) propagated, self-fertilizing (also called self-pollinated) and cross-fertilizing (also called cross-pollinated) crops.

Microgreens are tiny seedlings of some wild species as well as edible herbs, cereals, and vegetables. They are developed from the seeds of vegetables, legumes, herbs, and cereals and are regarded as immature greens (requiring light for photosynthesis and growing medium). They are picked shortly after the single set of true leaves has emerged from the cotyledon leaves. According to Xiao et al. (2012), microgreens have 4-6 times the nutritional density of their mature counterparts.