eChapters

This crop is gaining importance as a main ingredient in Indian diet due to its medicinal properties. Large areas are under its cultivation and the larger fruited varieties have become very popular. It is one of the prime constituents in ayurvedic preparations
Bark-eating caterpillars - Indarbela quadrinotata, I. tertraonis: Metarbelidae
The larvae of this species make zigzag galleries and feed on the bark under silken ribbon- shaped webs. They are stout and dirty brown in colour.
Fruit- borer : Deudorix Isocrates : Lycaenidae: The larvae of this beautiful butterfly cause considerable damage (up to 42%) to fruits.  They start feeding while the fruit is tender, and growing. During severe infestations, chemical control may be warranted. This can be done by spraying contact insecticides like malathion, or dichlorvos or  fenvalerate or other synthetic pyrethroids.

Origin and history  : Aonla or Indian gooseberry is also known as Indian amla. Aonla is the tropical South eastern Asia particularly central and southern India. It is commercially grown in Uttar Pradesh and Gujarat. In Uttar Pradesh aonla cultivation is more popular in Pratapgarh, Mirzapur and Barely. It is very rich from nutritional point of view being next to Barbados cherry in terms of vitamin C content (500-750 mg/100g pulp)which is not oxidized due to presence of tannins and even it is least destroyed during storage. Besides, it has an excellent medicinal value. It can be grown successfully in arid climate and in the soils of higher pH and poor fertility wastelands.

Nearly 70 percent of the cultivated crops all over the world are cross-fertilized and depend on insects like honeybees for pollination. At present, several countries are making desperate efforts to achieve self–sufficiency in food production by physical expansion of the area under cultivation, production of better quality seeds and animals, bringing more waste lands under cultivation, use of fertilizers,pesticides and irrigation. However, in the past decade or so, food production has come to a point of stagnation for some cultivated crops. Emphasis in the future therefore, should be on full use of under used resources. In order to maximize horticultural production, pollinators can be used as an important resource or input as improved variety of seed, timely irrigation, doses of fertilizers and plant protection.

1. APOMIXIS

Development of seed without fertilization. When the seed formation occurs without sexual fusion, the process is known as apomixis. This occurs by several mechanism, however all apomitic seed have genetic material only from the female plant Apomixis may or may not require pollination and pollen tube germination to initiate seed formation, however sexual union.

5.1 APOMIXIS

Apomixis is derived from two Greek words “Apo” (away from) and “mixis” (the act of mixing or mingling). It refers to the occurrence of an asexual reproductive process in the place of normal sexual processes involving reduction division and fertilization. During sexual reproduction, developmental steps occurring inside the ovule produce the female gametophyte (embryo sac) and following a double fertilization event give rise to embryo and endosperm structures. Apomixis can be best described as the reference to the asexual process. In some species, embryo sac is produced not as a result of meiosis and fertilization but by certain asexual processes. The occurrence of such asexual reproductive process in the place of normal seed reproductive processes of reduction division and fertilization is known as Apomixis. Such seedling plants produced in this manner is known as apomicts. ’’Apomixis’’ is used synonymously with ’’agamospermy’’ (Stebbins 1950), referring to the asexual production of seed.

Apomixis (asexual seed formation) is a phenomenon in which a plant bypasses the most fundamental aspects of sexual reproduction—meiosis and fertilization—to form a viable seed. Plants can form seeds without fertilization, and the seed genotype is consistent with the female parent. The development of apomictic technology would be revolutionary for agriculture and for food production as it would reduce costs and breeding times and also avoid many complications typical of sexual reproduction (e.g., incompatibility barriers) and of vegetative propagation (e.g., viral transfer). The application of apomictic reproductive technology has the potential to revolutionize crop breeding. Apomixis is widely distributed among higher plants. More than 300 species belonging to 35 families are apomictic. It is most common in Gramineae, Compositae, Rosaceae and Rutaceae. Among the major cereals maize, wheat and pearl millet have apomictic relatives. Apomixis is reproduction where only one parent passes genes to the offspring (King, 2006). Apomictically produced offspring are genetically identical to the parent (clones).The method is mostly seen in plants, especially flowering plants. Apomixis was defined by Hans Winkler (2008) as the replacement of the normal sexual reproduction by asexual reproduction, without fertilization. Propagation from cuttings or leaves, is not apomixis. Natural replacement of the seed by an asexual method is apomixis.

1). Scab
Diagnostic Symptoms
• Symptoms generally noticed on leaves and fruits.
• Affected leaves become twisted or puckered and have black, circular spots on their upper surface.
• On the under surface of leaves, the spots are velvety and may coalesce to cover the whole leaf surface. Severely affected leaves may turn yellow and drop.
• Scab can also infect flower stems and cause flowers to drop.
• Lesions later become sunken and brown and may have spores around their margins.
• Infected fruit become distorted and may crack, allowing entry of secondary organisms(Figure-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Ascomycota

It is a serious disease of apple which not only reduces the quality and size of fruit but also cause premature fruit drop and defoliation. Fruit loses its marketability due to poor quality. It also reduces storage time and cause loss up to 70%.

Apple codling moth: Cydia (Carpocapsa) pomonella: Tortricidae 

This is an international pest and is now seen in Kashmir, Himachal Pradesh and Tamil Nadu. This is a fruit borer whose moth is silvery white with a dark band on the margin of the forewing and light brownish hind wings with a fringed margin. The moths hold their wings roof-like over the body while at rest.

Biofertilizers are preparations containing micro-organisms, with capability of mobilizing nutritive elements from non usable form to usable form through biological processes. Bio-fertilizers have definite advantage over chemical fertilizers. Chemical fertilizers supply over nitrogen whereas bio-fertilizers provide in addition to nitrogen, certain growth promoting substances like hormones, vitamins, amino acids, etc, crops have to be provided with chemical fertilizers repeatedly to replenish the loss of nitrogen utilized for crop growth. Bio-fertilizers application in agriculture will have greater impact on organic agriculture and also on the control of environmental pollution, soil health improvement and reduction in input use. Farmers try to increase yields of vegetable crops by mean of heavy nutrition’s. The use of chemical nitrogen and phosphorus fertilizers at high levels had an adverse effect on the accumulation of NH4+, NO3-, NO2- and PO4- in vegetable product tissues. Therefore, clean agriculture recently depends upon using biofertilizers as well as organic in order to produce high yields with the best commodity quality without contamination and less accumulation with heavy metals.

The application of high input technologies such as chemical fertilizers, pesticides, herbicides have improved the production but there is growing concern over the adverse effects of the use of chemicals on soil productivity and environment quality. Thus, integrated nutrient management has become an accepted strategy to bring about improvement in soil fertility and protecting the environment. This strategy utilizes a judicious combination of fertilizers, organic manures and bio-fertilizers. Biofertilizer is a natural product carrying living microorganisms derived from the root or cultivated soil. So they don’t have any ill effect on soil health and environment. Besides their role in atmospheric nitrogen fixation and phosphorous solubilisation, these also help in stimulating the plant growth hormones providing better nutrient uptake and increased tolerance towards drought and moisture stress. A small dose of biofertilizer is sufficient to produce desirable results because each gram of carrier of bio-fertilizers contains at least 10 million viable cells of a specific strain. Biofertilizers means the product containing carrier base (solid or liquid)

Mushrooming population, subsiding agricultural resources; land and water, people’s concern towards quality of vegetable s/food and changing climate throws several challenges for vegetable production. Conventional management and crop improvement strategies alone can no longer sustain the nation’s as well as global demand. So there is an urgent need to adopt modern biotechnological methods which are multidisciplinary where a large number of tools and elements of conventional breeding technique, genetic engineering, molecular biology, molecular genetics, bioinformatics and biochemistry are utilized and integrated. Biotechnological tools provide three major aspects of genetic improvement viz, application of tissue culture, molecular breeding or marker assisted breeding and genetic engineering.

Introduction

Cell and tissue culture techniques are becoming increasingly popular as alternative means of plant vegetative propagation. Plant tissue culture involves asexual methods of propagation and its primary goal is crop improvement. The success of many in vitro selection and genetic manipulation techniques in higher plants depends on the success of in vitro plant regeneration. Crop breeding and rDNA technology require the widespread use of reliable true to type propagation and better regeneration methods. Plant regeneration can be grouped in to different categories of enhanced release of axillary bud proliferation, organogenesis and embryogenesis. The oldest practical application of tissue culture techniques is the propagation of disease free plants from meristem tip culture. Meristem tip consisting of dome and one or two subjacent leaf primordial with a size up to 0.25 mm are aseptically cultured in appropriate nutrition media. The resulting plants are disease free because the pathogen is not generally present in the meristematic cells.

The aim of germplasm conservation is to ensure the availability of useful germplasm at any time. Breeding programs rely heavily on locally adapted ancient plant varieties and their wild relatives as sources of germplasm. Further, the continuing search for high yielding varieties of crop plants with resistance to pathogens and pests warrants the availability and maintenance of large collections of germplasm. In species which are propagated through seeds, it is economical to preserve the seeds. These seeds are dried to water content of 5 - 8% and then stored at a temperature of -18° C or lower and low humidity. In case of vegetatively propagated species, preservation of germplasm heavily taxes manpower and land resources. As in vitro techniques are becoming more important in crop improvement through the use of somatic cell genetics, the material produced in vitro will have to be conserved in vitro. The in vitro system is extremely suitable for storage of plant material, since in principle it can be stored on a small scale, disease free and under conditions that limit growth. Germplasm storage in vitro is crucial for the future development and safety of agriculture.

1. INTRODUCTION

‘Fertigation’ is the technique of supplying dissolved fertilizer to crops through an irrigation system (Haynes, 1985). When efficient irrigation system combined with both nutrients and water, it can lead to obtain higher yield and preferable quality desired marketable produce.

Often, side-dressings of straight solid fertilizers are found to be the management constraints. Most of the growers have the experience of how much the applied fertilizer is either washed from the soil in run-off or leached below the root zone.

Continuous small applications of soluble nutrients have the benefits like labour, due to fertilizer being placed uniformly around the plant roots and allow savings/economy rapid uptake of nutrients by the plant.

The “falling number” is a measurement used by plant breeders and grain quality specialists to determine the soundness of wheat, which is primarily influenced by the degree of sprouting in a grain sample. Hagberg Falling Number is the full name of this international standard of sprout measurement.  The term “falling numbers” refers to the amount of time it takes for the Hagberg steel ball in this sprout analysis tool to fall through a flour/water slurry that has been heated to release the starch from water. 

Black Pepper and Pearl Millet Flour for Disease Control in Chickens: To treat a disease in chickens characterized by frothing at the mouth, frequent cackling, restlessness, and reduced food intake, 10-15 black pepper corns are ground and mixed with pearl millet flour and water to form small pellets. These pellets are fed to the chickens to increase body heat and help eliminate the disease (Sabarkantha, Gujarat) (Parmar, 1992a).

Use of Garlic Against Pulorum in Poultry Birds: To cure chicks affected by Salmonella pulorum, one or two peeled cloves of garlic (Allium sativa) are soaked in a cup of water for 12 hours. The water is then given to the chicks to drink, curing them in two days (Dahod, Gujarat) (Khant, 1999).

Poultry Disease Management: For treating an unidentified disease in poultry birds that kills them within a few days, tribals use various plant-based remedies:

1. Bark of ‘beda’

2. Small pieces of onion

3. Grains of paddy smeared with kerosene

4. Tuber of ‘dev-alad’ (Curcuma spp)

11.1 Control of Diarrhea in Animals Using Traditional Remedies

1. Strychnos potatorum Leaves: In Dahod, Gujarat, approximately 100g of kantas (Strychnos potatorum) leaves are crushed and soaked in water. This mixture is given to the ailing animals for three days (Sitaram, 1997).

2. Ventilago denticulata and Syzygium cumini Mixture: In Bori, Shahera, a mixture of asul (Ventilago denticulata) roots and jamun (Syzygium cumini) bark is crushed and administered with water to the suffering animal. This treatment typically cures diarrhea within 2-3 days. Asul is a creeper found year-round in hedgerows, and jamun is a common tree (Dalsukhbhai, 1993).

3. Decoction of Soymida febrifuga: In Shahera, Gujarat, fresh leaves of royan (Soymida febrifuga) are crushed and soaked in water for one hour. A 100-150 ml dose of this concoction is given to the animal, which usually brings relief within 24 hours. This remedy has a long history in the region (Dalsukhbhai, 1993).

4. Ficus hispida: In Panchmahal, Gujarat, roots of dedhumari (Ficus hispida) are washed, crushed, and soaked in water for one hour. The mixture is then filtered through cloth, and 100-200g of the filtrate is administered to the animal. The treatment typically takes effect within a day and completely cures the animal in about 2-3 days. Many farmers use this method, although they may also consult veterinary experts (Baria, 1993).

5. Umra Bark and Banana Plant: During the monsoon, diarrhea in animals is treated using umra bark and banana (Musa Paradisiaca) plant sap. About 500g of umra bark is pounded and mixed with 500ml of banana stem juice. The mixture is filtered, and the filtrate is given to the affected animal. One hour later, the animal is fed 250ml of groundnut oil, resulting in a cure within a day or two (Valsad, Gujarat) (Patel, 1996).

1. Use of banana pseudostem in fish pond to enhance productivity of f ish: Pseudostems of banana, after harvesting the bunch, are added to the pond by cutting longitudinally, which increases the pH and oxygenation of pond water. This practice increases the fish yield. As it involves low cost, it is being followed by 80% of the farmers in Bastar village of Balasore district in Odisha.

2. ITKs on Craft and Gear Making / Maintenance (Vipinkumar et al., 2013): The major ITKs of scientific rationale identified in the craft and gear making and maintenance were ‘Sardine oil smearing on craft wood for improving the durability’, ‘Smearing cashew kernel oil on craft wood for durability’. Similarly, the durability of cotton nets was believed to be improved by dipping in banpu bark decoction. In the same way, beating nets with thalampu plant for ensuring strength was another one identified in craft and gear maintenance. The scientific validity assessment indicated that the boats of about 6.5 ft size length and 4.5 ft width require 3.5 to 4 litres of oil of sardine. The oil coasts about Rs 70/- per litre. Three cosequitive coatings followed by natural drying under sun are required for good results. Cashew smearing: The boats of about 6.5 ft size length and 4.5 ft width require 3.5 to 4 litres of cashew nut oil and the oil costs about Rs 40 -50/- per litre. Here also three cosequitive coatings followed by natural drying under sun are required for good results. Smearing Cashew kernel oil on craft wood for durability in Karwar of Karnataka is shown in photograph 1. For improving durability of nets, dipping nets in banpu bark decoction was practiced. The thalambu beating is nowadays not practiced due to the non-availability of those items. Instead, fisherfolk use a powder available in the market especially in the net shops. 1 kg of the colour powder (red/ blue) is required for 1 kg of net. The advantage is that the wastage due to mud coating and dirt can be rectified to a great extent.

Introduction

Genetic markers have assisted in genomic characterization of various crops species (Rafalski and Tingey, 1993). Most of the genetic marker systems rely upon DNA sequence polymorphisms (Staub et al., 1996). Each system has unique advantages and disadvantages based on cost, experimental objectives and type of information desired. Regardless of its inherent methodology, the effective use of any marker system for genetic analysis requires reliability and consistency.

The random amplified polymorphic DNA i.e. RAPD (Williams et al., 1990) or arbitrarily primed polymerase chain reaction (Welsh and McClelland, 1990) marker identifies DNA sequence polymorphisms. In this technique, segments of genomic DNA are amplified in a polymerase chain reaction i.e. PCR (Mullis et al., 1986) with random primers followed by agarose gel electrophoresis. This is simple, relatively inexpensive and is amenable to high throughput investigation. RAPD markers are usually dominant and polymorphisms are observed as the presence or the absence of DNA bands of a particular size. RAPDs are common molecular approaches employed in plant breeding, genotypic differentiation, gene linkage analysis, assigning evolutionary and taxonomic affinities and relatedness and DNA fingerprinting analysis (Lin et al., 1996).

ABSTRACT

The discovery of polymerase chain reaction method of amplification of DNA is an important milestone in molecular genetics and used for multiple purpose such as construction of linkage maps, marker saturation of specific genome regions, analysis of genetic diversity, molecular phylogeny and cultivar identifications (Williams et al; 1990, Demeke, Adams and Chibbar; 1992, Welsh and Maclelland; 1990;, Obara-Okeyo and Kako; 1998, Sawhoon Lin et al; 1998 and Grunger et al; 1998.). 15 species of three morphologically distinct groups of Orchids belonging to the subfamily Epidendroideae and tribe Dendroebieae and Cymbideae were studied using random amplified polymorphic DNA (RAPD) technique. Genetic variability and relation between these 3 genera and 15 species were estimated based on band sharing and cluster analysis. A total of 227 distinct major RAPD bands of which 97% were polymorphic were generated from 15 arbitrary primers. The number of bands generated per primer ranged from 3 (Primer A-10) to 22 (Primer K-10 and N-17) with a mean of 14.8 bands per primer. Out of 227 bands generated 222 bands (97.8%) where polymorphic while only 5 bands were monomorphic. The primer resolving power was found to range between 2.00 (prime A-10) and 13.38 (primer k-10) when all the species were studied were taken into account. The molecular analysis grouped all the species into 5 groups. The polymorphic patterns generated by RAPD profiles showed different degrees of genetic relationship among the species studied and the RAPD marker were found to be an useful tool for detecting genetic variation within the species of three important genera of Orchids.

Greenhouses have become an essential part of modern agriculture as they offer controlled environments for plant growth, research, and horticultural practices. This chapter discusses the various applications of greenhouses, which include extending growing seasons, improving crop yields, supporting botanical research, and producing ornamental plants. Additionally, it emphasizes the importance of repair and maintenance in greenhouse operations. Understanding the repair and maintenance protocols is crucial to ensure the sustained functionality of heating systems, water management, and structural components.

1. Introduction

2. Human Genome Project

3. Development of Bioinformatics

3.1. Indian scenario

4. Plant Genome Model

5. Components of Plant Genome Database

5.1. Stock centre databases

5.2. Genome mapping database

5.3. DNA sequences

6. Agricultural Research Service and National Research Initiative

6.1. Objectives of plant genome programme

6.2. GIBN Trans – pacific digital library experiment plant genome databases

7. Plant Genome Databases

7.1. Vegetable genome databases

7.1.1 Mendel-Plant Gene Nomenclature Database

7.2. Others

7.2.1. Bean Genes – Phaseolus / Vigna sp. Database

7.2.2. Solgenes Database

7.2.3. Soybase

7.3. Other data bases

7.3.1. PGDB (Pathway genome data base)

7.3.2. Demeter’s genome

8. Tools of Bioinformatics

9. New Directions in Genome Database at Stanford

10. Navigating the World Wide Web

11. Virtual Genome Centre (VGC)

11.1. Programmes at the VGC

12. Impact of Gene Chips and Micro Arrays

13. Applications and Impacts

14. Bottlenecks

15. Future Thrust