eChapters

Chilli (Capsicum annuum L.) and bell pepper (Capsicum annuum var. grossum) are economically significant crops in India, known for their distinct attributes and culinary uses. Despite their shared progenitor, these crops exhibit varietal differences and require specific breeding strategies for hybrid seed production due to small flower size. The development of male sterile systems has revolutionized hybrid seed production, with the exploitation of Genetic Male Sterility (GMS) and Cytoplasmic Male Sterility (CMS) in chilli and bell pepper breeding. The market for chilli seeds in India is substantial, driven by the demand for high-value hybrid seeds. This chapter comprehensively reviews the botanical characteristics, genetic resources, and breeding objectives in Capsicum species, emphasizing traits like disease resistance, high yield, and stress tolerance. It also highlights varietal achievements in public and private sectors, showcasing improved cultivars and hybrids with specific attributes. The integration of molecular markers in male sterile genes facilitates efficient hybrid seed production, ensuring genetic purity and quality. Additionally, the package of practices for seed production in chilli underscores the importance of isolation, field standards, inspection, harvesting, seed processing, and quality standards to maintain seed purity and viability.

Advances in seed production techniques have revolutionized brinjal cultivation, enhancing productivity, quality, and sustainability. Traditional methods, such as manual emasculation and pollination, have been supplemented and in some cases replaced by innovative approaches, contributing to increased efficiency and reduced labor costs. Cytoplasmic male sterility (CMS) and grafting technologies have emerged as valuable tools, offering novel avenues for enhancing breeding efficiency and managing soil-borne pathogens. These advancements have facilitated the development of high-yielding F1 hybrids and improved pure-line varieties with enhanced resilience to pests and diseases. Furthermore, the adoption of precision agriculture techniques, including controlled environment agriculture and hydroponics, has enabled year-round seed production, mitigating the impact of seasonal fluctuations and adverse weather conditions. Integrated pest management (IPM) strategies have been integrated into seed production protocols, minimizing chemical inputs and promoting environmentally sustainable practices. Additionally, innovations in seed extraction methods, such as mechanical extraction and dry extraction techniques, have streamlined the seed processing workflow, resulting in higher seed purity and improved germination rates.

Tomato (Solanum lycopersicum L.) is one of the most commercially important vegetable crops globally, with a significant demand for high-yielding hybrids and improved open-pollinated varieties. Efficient seed production methodologies play a crucial role in ensuring the availability of genetically pure and high-quality seeds for commercial cultivation. Traditional hand emasculation and pollination remain widely used despite being labor intensive, given their economic feasibility due to high seed yield per fruit. Hybrid seed production is further refined through mechanized pollen collection and controlled pollination techniques, enhancing efficiency. Seed quality management, including rigorous isolation protocols, field inspections, and breeder and foundation seed production standards, ensures genetic purity and viability. Advances in molecular breeding and the application of marker-assisted selection (MAS) have enhanced the efficiency of parental line development and hybrid seed production. This chapter provides an in-depth review of tomato seed production methodologies, covering botanical characteristics, genetic resources, and breeding objectives aimed at improving disease resistance, abiotic stress tolerance, and fruit quality traits. It further discusses varietal advancements in the public and private sectors, highlighting elite hybrids and open-pollinated cultivars tailored for different agro-climatic conditions. Additionally, the package of practices for seed production in tomato underscores the importance of isolation, field inspections, seed extraction methods, processing techniques, and quality assurance standards to maintain genetic purity and seed viability.

56.1. Aim

To determine quality characteristics of dried and dehydrated fruits and vegetables.

56.2. Theory

The quality of dried/dehydrated products can be measured by estimating moisture contents, preservatives level, acid insoluble ash contents and most importantly enzyme reactions.

56.3. Estimation of Acid insoluble ash

1. To total ash add 25 ml of dilute HCl (10% w/w)

2. Cover with a watch glass and boil gently over a low flame for 5 minutes

3. Filter and wash the filter paper thoroughly with hot water

4. Return the paper to the original dish, ignite, cool and weigh the insoluble ash

55.1. Aim

To determine dietary fibres in given samples.

55.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre.

55.3. Procedure for estimation of dietary fibre

55.3.1. Acid detergent fibre (ADF)

Reagents: Cetyl tri-methyl ammonium bromide (CTAB) reagent and Acetone

Apparatus: Digestion apparatus vacuum pump, oven, desiccator and sintered glass crucible.

54.1. Aim

To determine crude fibre in given samples.

54.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre (Cellulose + lignin + hemicelluloses + pectic substances). Crude fibre is the organic residue of a sample. A fat free sample is treated with boiling sulphuric acid and subsequently with sodium hydroxide. The residue after subtraction of ash is regarded as crude fibre.

54.3. Reagents/ Apparatus/Glassware

1. 0.255 N Sulphuric acid solution

2. 0.313 N Sodium hydroxide solution

3. 10% Potassium sulphate solution

4. Ethanol

53.1. Aim

To conduct practical on freezing of vegetables

53.2. Theory

Freezing is often considered the simplest and most natural way of preservation for vegetables. Frozen vegetables and potatoes form a significant proportion of the market in terms of frozen food consumption. The quality of frozen vegetables depends on the quality of fresh products, since freezing does not improve product quality. Pre-process handling, from the time vegetables are picked until ready to eat, is the important factor affecting quality of finished product. The process variables for freezing of different vegetables and flow chart are shown in Table 54.1 and Fig 54.1. Generally fruits after preliminary treatments are packed in sugar syrup and frozen in freezer.

52.1. Aim

To conduct practical on freezing of fruits.

52.2. Theory

Freezing is a method of preservation in which the food temperature is reduced below freezing point and a proportion of water changes in to ice-crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause lowering of water activity in the food. Thus, reduction in water activity and use of low temperature coupled with some pre-treatments is the basis for food preservation by freezing. The method for freezing of fruits depends upon the intended use. The process variables for freezing of different fruits and flow chart are shown in Table 52.1 and Fig 52.1. Generally fruits after preliminary treatments are packed in sugar syrup and frozen in freezer. The fruits are frozen to an internal temperature of -18oC or lower and kept at 18oC or lower throughout transport and storage.

51.1. Aim

To prepare products from tomato fruits.

51.2. Theory

Commercial products from tomatoes include ketchup, soup, cocktail, chutney, canned and dehydrated tomatoes. Processing of tomato is now practiced from home scale level to a large scale enterprise. Both puree and paste are used for preparation of different finished products like ketchup, canned and dehydrated products, soup etc. The method for preparation of these products are well standardized, however, some modification with respect to recipes are made in the processing plants owing to variation in the quality of the raw material. The method for preparation and recipe of different tomato products are as under:

51.3. Raw material, ingredients and utensils required

1. Tomatoes, sugar, salt, sodium benzoate etc.

2. Stainless steel knives, utensils, juicer, pulper, glass bottles, corking machine, crown corks, muslin cloth, boiler/gas bhatti, etc.

50.1. Aim

To prepare products from tomato fruits.

50.2. Theory

Commercial products from tomatoes include juice, puree and paste. Processing of tomato is now practiced from home scale level to a large scale enterprise. As a semi finished product, tomato puree is prepared on a small scale while at large scale tomato paste has gained commercial significance. Both puree and paste are used for preparation of different finished products like ketchup, juice, soup etc. The method for preparation of these products are well standardized, however, some modification with respect to recipes are made in the processing plants owing to variation in the quality of the raw material. The method for preparation and recipe of different tomato products are as under:

50.3. Raw material, ingredients and utensils required

1. Tomatoes.

2. Stainless steel knives, utensils, juicer, pulper, glass bottles, corking machine, crown corks, muslin cloth, boiler/gas bhatti, etc.

49.1. Aim

To perform practical on drying/dehydration of different vegetables.

49.2. Theory

Drying refers to the method of removal of moisture content from the food to a level at which the activities of food spoilage and food poisoning micro-organism are inhibited. Therefore, reduction in water activity of the food is the main principal of preservation by drying. In the dehydrator initial temperature is generally kept at 43oC which is then gradually increased to 60-66oC for vegetables. The product shall be prepared from wholesome vegetables free from blight, insect infestation and fungal discoloration. Only edible portion of the vegetables shall be used and it shall be free from stalks, peels, stems, and extraneous matter. The dried/dehydrated vegetable may contain permitted preservative. The finished product shall be of good edible quality and shall reasonably reconstitute to its original shape and quality on boiling for 15 minutes to an hour. The finished product shall be free from visible mould, insect or larvae. Kind of dry vegetable shall be declared on the label. Acid insoluble ash shall not be more than 0.5%.

48.1. Aim

To conduct practical on drying of fruits

48.2. Theory

Drying generally refers to the method of removal of moisture from the food under natural condition such as sunlight and wind for example open sun drying, shade drying etc. Dehydration refers to a process of removal of moisture by application of artificial heat under controlled conditions of temperatures humidity and air flow. It also represents drying of agricultural commodity to dry state. For drying, single thin layer of fruit and vegetables, either whole or sliced after primary pre-treatments is spread on the trays which are then placed inside the dehydrator or in the open sun for drying. In the dehydrator initial temperature is generally kept at 43oC which is then gradually increased to 66-71oC for fruits.

47.1. Aim

To conduct practical on preparation of pickle from different vegetables.

47.2. Theory

Pickles are good appetizers and add to the palatability of a meal. Pickles may be unfermented (mostly Indian pickles) and fermented (western pickles). Salt, vinegar, edible oil or acetic acid acts as preservative in pickle making. Mixed vegetable pickle from cauliflower, carrot and turnip is most common. Cucumber and olive pickles are examples of fermented pickles. The process of steeping vegetables/fruits in salt solution is also a common method used for preservation of mango and vegetable slices for longer duration storage for its later use in pickling.

46.1. Aim: To prepare pickle from different fruits.

46.2. Theory

The preservation of food in common salt (NaCl) or in vinegar or edible oil with the addition of spices and condiments is known as pickling. It is one of the most ancient /oldest methods for preservation of fruits and vegetables. Salt, vinegar, edible oil or lactic acid act as preservative in pickle making. Several kinds of pickles are sold in the Indian market. Mango pickle ranks first followed by lime, lemon, mushroom, galgal, aonla, jackfruit and karonda pickles.

Brining: For pickling, unripe fruits (like mango) after preparation (peeling, slicing) are mixed with salt or brine for its later use in pickle preparation

45.1. Aim

To conduct practical on preparation of sauce from different fruits.

45.2. Theory

Sauces are of thinner consistency as compared to ketchups and contain TSS not less than 15oBrix. Pumpkin, tomato, apple, papaya and plum pulps etc are used successfully for preparation of sauces. Mixed vegetable sauce using pumpkin, tomato, chillies, carrots are sold as continental sauces.

45.3. Raw material, ingredients and utensils required

1. Fruits like Apple, tomato, papaya, pumpkin, plum etc are used for making sauce.

2. Stainless steel knives, de-corers, ladle and utensils for cooking and mixing, glass bottles, sterilization tank, Bhatti/LPG stove etc.

44.1. Aim

To conduct practical on processing of tea and coffee.

44.2. Theory

Tea is an evergreen woody perennial grown in many Asian countries including China, Japan, Java, Sumatra and India. The plant produces dark green, small shiny leaves with white blossom. In post harvest and manufacturing practices for tea, only mechanical and physical processes are allowed with natural fermentation. It can be manufactured by orthodox, CTC (crush, tear and curl) processing.

Coffee is an important beverage used all over the world. Brazil and Columbia are the largest coffee producers in the world. Coffee is harvested in one of two ways; strip picking (entire crop is harvested at one time either by machine or by hand); selective picking (only the ripe berries are picked individually by hand) during dry season when cherries are bright red, glossy and firm. After picking, the coffee can be prepared either by dry method which produces natural coffee or by wet method which produces washed coffee.

43.1. Aim

To conduct practical on preparation of preserve and candies from fruits.

43.2. Theory

Preserves (murraba) are made by cooking the entire fruit or its slices/pieces in sugar syrup of higher concentration (65-70°Brix). Usually for 1 kg fruit around 1.75 kg sugar is used and the cooking is continued till the TSS reaches 68%. Fruits like aonla, bael, apple, pear, karonda, pineapple and papaya etc can be used for making preserves. Aonla, bael and apple preserves are commercially available in the market.

Candies are also prepared in similar way as preserve, but the TSS maintained is slightly higher (75-80%). In preserve, the fruit is kept immersed in syrup and packed while in case of candies, the syrup is drained off and fruit pieces are further dried and packed. For candy preparation, the fruit pieces are dipped initially in 55-60% sugar solution and the TSS of the syrup is increased by 5% everyday up to 4-5 days till a TSS of 70oBrix is reached. The fruit pieces are then separated from syrup, rolled in powdered sugar, dried and packed. Karonda, apple, peels of orange, lemon, grapefruit, petha (Ash gourd) and ginger etc are used for candy preparation. Petha, ginger, papaya, aonla etc candies are commercially available in the market.

42.1. Aim

To conduct practical on preparation of chutney from different fruits.

42.2. Theory

Fruit chutney is made in the same way as jam except that the spices, salt and vinegar or acetic acid is also added. In comparison to jam, chutney contains less total soluble solids. The product shall be of good quality with palatable and appetizing taste. As per FSSAI specifications, the chutney shall contain minimum of 40% fruit (w/w) in the final product with total soluble solids not less than 50%. The acidity in the final product shall not be more than 2 percent with ash content not exceeding 5 percent. Mixed fruit chutney is commercially made by using different fruits in appropriate combinations.

42.3. Raw material, ingredients, equipments and utensils required

1. Stainless steel peeling/cutting knives, pulper for extraction of pulp.

2. Utensils for cooking and mixing, ladle, glass jars, sterilization tank, diesel or LPG stove/bhatti etc

41.1. Aim

To prepare marmalade from citrus fruits.

41.2. Theory

Marmalade is a fruit jelly in which the slices/shreds of peel are suspended. The marmalades are prepared generally from citrus fruits like orange and lemon. The pectin and acid contents of the marmalades are kept slightly higher than that for jellies. They shall contain minimum of 45% fruit and 65% total soluble solids.

41.3. Raw material, ingredients, machinery required

1. Orange and lemon like citrus fruits are used for making marmalade.

2. Stainless steel knives, utensils for cooking and mixing, muslin cloth, glass jars, sterilization tank, gas bhatti etc.

40.1. Aim

To prepare jelly from guava fruits.

40.2. Theory

Jelly is prepared by boiling the fruit with or without addition of water, straining the extract and cooking the clear extract with sugar to a stage at which it will set to a clear gel. The jelly should be transparent, well set, but not too stiff and having original flavour of the fruit. It should be attractive in colour and should keep its shape. In the preparation of jellies, pectin is the most essential constituent. As per FSSAI specifications, the quantity of fruit and soluble solids in the final product shall not be less than 45 and 65 percent (w/w) respectively. Guava jelly is the commercial product available in the market however jelly can be prepared from sour apple, karonda, jamun, loquat etc.