eChapters

Abstract

Studies were carried out on 110 seedling trees of aonla growing at Tikker area of district Sirmaur, Ghanatti area of district Shimla and Kandaghat & Arki areas of district Solan to know the extent of divergence for various fruit characters by using non-hierarchical cluster analysis. Maximum co-efficient of variability was observed for fruit volume (38.63 %). First component gave maximum Eigen root value (7.286) and maximum percent variation (45.54 %). All 110 genotypes got clustered into eight clusters and the distribution of genotypes from different location was random. Maximum genotypes (30) entered in cluster 3 and minimum (5) in cluster 8. Intra-cluster distance varied between 1.877 (Cluster 5) to 3.137 (Cluster 8). The inter-cluster distance ranged between 3.006 (Cluster 3 & 4) to 11.677(Cluster 5 & 8). Wider genetic base is the prerequisite for breeding to get better recombinants and segregants. So, genotypes of Cluster 5 & 8 can be used for future aonla breeding programmes.

Twitching is a condition occurring predominantly on the surface of the fruit which is exposed to direct sunlight; however, it is not well understood what the source of the disease is. Growth in immature fruits cannot be detected during later stages of fruit maturity. On cv. Golden Solo Party there are a few examples. This is termed the “frog skin” or “fox skin.” It shows on the fruit’s surface as the Dark Brown Flecks. 

Abstract
The analysis of variance showed significant differences for all the traits studied. The best two combinations out of the 28 F1s produced by crossing eight diverse lines of capsicum in diallel fashion (excluding reciprocals) with respect to most of the traits were Osh Region x EC-119058 and California Wonder x Bonali.

Keywords: Heterosis, Capsicum annuum L.

Bell pepper (Capsicum annuum L.) also known as Sweet pepper or Spanish pepper is an important vegetable used in various delicacies and salads, the world over. The crop originated in New World tropics and subtropics (Greenleaf, 1986), and introduced in India by the British. Because of its excellent quality as obtained under Shimla hills conditions, it came to be called as Shimla Mirch. Its most notable feature is flavour and hence is appreciated worldwide. It is also an excellent source of pro-vitamin A and vitamin C. Bell pepper assumes a special significance in the mid hills of Himachal Pradesh as it is grown as an off season crop during summer and rainy seasons, which brings lucrative returns to the growers. 

Snapmelon [Cucumis melo L. var. momordica (Roxb.) Duthie * Fuller] originated  in India. It was intensively cultivated in the 19th century in Northern India (Duthie 1905) where it is commonly called as ‘Phut’ which means to split or crack. Immature fruits are cooked or pickled and the low sugared mature fruits are eaten raw. The mature fruits crack or burst. Fruit cracking is  either longitudinal or random starting in middle of fruit (Fig.1).

Seed

A seed is a mature ovule consisting of an embryo together with stored food, all enclosed by a protective coat. It is product of the ripened ovule of gymnosperm and angiosperm plants which results as a consequence of fertilization and some growth within the mother plant formation of seed completes the cycle of reproduction. The embryo develops from zygote and the seed coat from the integument of ovule. A seed has three main parts these are seed coat, endosperm and embryo. Embryo includes the root, the cotyledon and embryonic leaves. Seed is located inside fruit and a fruit is a mature ovary. In broad sense any plant part which is used for commercial multiplication of crop is called seed. Thus in case of potato stem and tuber are also known as seed. Improved seed results in (1) Better germination (2) Vigorous seedling growth (3) Higher crop stand (4) Better quality of produce (5) Ultimately in higher crop yield.

Development of seeds: Successful pollination and fertilization are the prerequisites to produce a viable seed. During pollination the pollen grains are transferred from anther to the stigma and get stuck there in the sticky fluid secreted by the stigma. The fluid contains sugar which induces germination of pollen grains. The pollen tube grows downward through the style in the ovary until it reaches the embryo sac and discharges two male gametes-one is fused with a female gamete and forms the zygote (2n) and the other is united with two pollar nuclei to produce endosperm (3n). The zygote develops and gives rise to embryo embedded in nutritive tissue developed from endosperm. The nucellus tissues surrounding these structures provide necessary energy for their proper development. Like fruit, the seed also exhibits sigmoid and sometimes double sigmoid pattern of growth. Various changes occur during the course of seed development and the sequences of development may vary from species to species keeping the basic theme unaltered.

Naïve panel: It refers to the sensory evaluation panel that has not been trained specifically regarding the product evaluation being undertaken in the study.

Nano technology: It is the art of manipulating materials on an atomic or molecular scale to build microscopic devices. The word “ nano’ meaning ‘dwarf” in Greek language refers to dimension / magnitude of the material / particle in the order of 10⁹. Nanotechnology is nothing but manipulating of particles into very small particles called nano-particles. These particles have dimensions in the scale of nano-meter and used to design / build materials and devices. Nanotechnology has been provisionally defined as relating to materials, systems and processes which operate at a scale of 100 nanometers (nm) or less. It will allow many things to be manufactured at low cost and with no pollution. The concept of nanotechnology was first given by Nobel Laureate physicist Richard P. Feynman in Southern California in 1959.

NaNO_2: Pink colour of cured meat is preserved by NaNO₂. It is effective against Cl. Botulinosm

Nanogram: It is one billionth of a gram.

ABSTRACT

The present experiment was conducted in order to develop a protocol for rapid in vitro propagation of pomegranate Cv. Mridula. Shoot tip and nodal segment explants selected from mature plant were cultured in MS medium supplemented with NAA and BAP in different concentration and combinations. To control phenolic browning, sub- culturing of cultures was under taken at an interval of first and third day after inoculation. The MS medium supplemented with NAA 0.4 mg/l + BAP 1.0 mg/l showed highest percentage of shoot differentiation with 77.77 per cent and 81.25 per cent response from shoot tip and nodal segment explant respectively. In the same media, earlier shoot initiation as well as more number of multiple shoots/culture were obtained. The shoots when elongated up to 2.5 - 3.0 cm length were transferred for rooting in the ½ strength MS media supplemented with auxins. There was no significant difference for root induction among the type of auxin used either NAA, IBA or both in combination. The concentration of auxins 0.5 mg/l was found to be optimum. Rooted plantlets after hardening in green house gave 49 per cent survival.

The hot arid regions of India are spread over 32 million hectares in the states of Rajasthan (61%), Gujarat (20%), Punjab (5%), Haryana (4%), Karnataka (3%) and Andhra Pradesh (7%). The arid regions of western Rajasthan experience an annual rain fall between 100-500 mm with a coefficient of variations varying from 40-70 per cent. Low and erratic rainfall coupled with extreme temperature results in complete or partial crop failure, considerably affects the agricultural economy of the region. High wind velocity causes wind erosion and shifting of sand dunes covers the fertile agricultural land and canals (Rao and Singh,1998). The rise in temperature begins from March onward and attains its peak in May as high as 46°C. The transpiration is low during winters but during summer in months (May & June), it reaches up to 16 mm per day. The annual estimated potential evapotranspiration values ranges from 1600 mm in eastern part and 1800 mm in western part of the region.

In the arid regions of Rajasthan, Haryana, Punjab and eastern part of arid Gujarat the soils have developed from alluvial and aeolian parent materials. In context to the type of soils light brown sandy soils with dune dominance is 30.6 per cent and associated others 34 per cent area, light brown soils 1.7 per cent, Grey brown soils 13.6 per cent hard pan in 5.9 per cent and seirozems in 1.7 per cent area (Dhir, 1977). Kolarker et al. (1989) reported that in 12 district of Rajasthan sandy soils dominated in 14.3 million ha. These soils are poor in fertility very low organic carbon (0.03-0.1%), light in texture called desert soils (north western) in the order of aridisols. It has undulated topography with the dominance of sand dunes inter-dunal plains. About 80 per cent area falls under rain fed cropping and moth bean, cluster bean, pearl millet, and sesame are the main crops grown during kharif season after the onset of monsoon. Under the vagaries of monsoon and recurrent drought the rain fed cropping becomes a gamble, which creates severe food and fodder scarcity in the region.

The purpose and the mode of marketing of vegetables determine the type of vegetable gardening. The various types of vegetable gardening are as follows.

Home or Kitchen gardening

Market gardening

Truck gardening

Processing gardening

Vegetable forcing

Seed production gardening

Floating vegetable gardening

Abstract

It is essential that care is taken in the design of primers for PCR. Several parameters including the length of the primer, percentage of GC content and the 3' sequence need to be optimized for successful  PCR. Some of these parameters can be easily manually optimized while others are best done with commercial computer programs. In any event, careful observance of the general rules of primer designing will help ensure successful experiments.

Keywords: Bioinformatic tools, primer designing

ABSTRACT

Shoot organogenesis from several explant types excised from in vitro grown plants of local elite brinjal (Solanum melongena L.) cv. Co2 was studied in response to the supplementation of thidiazuron (TDZ), BAP, IAA and NAA. Cotyledons and leaves were found to be the most responsive explants. High shoot bud induction rate (>50 shoot buds/explant) was achieved in the presence of 0.044 mg/l TDZ. The organogenic calli were subcultured in the same shoot bud induction medium for shoot development. Among different growth hormones, TDZ @0.044 mg/l induced more number of shoots per explant compared to BAP, IAA and NAA combinations. Efficient rooting was observed on half strength MS medium supplemented with 0.5 mg/l IAA. Higher percentage of rooting was achieved only when the well developed shoots were directly transferred to soil.

Abstract

Seven different parents viz. July Elberta, Alton, J.H. Hale, Kanto-5, Saharanpuri, Quetta as female and Kateroo as male were used in the present crosses.  The crosses were made in the year 1997 and 76 hybrid seedlings under different crosses were raised  for these studies. The variability in respect of chemical characters, the mean values for titratable acidity for each cross varied between 0.50 to 0.75%, TSS 11.53 to 12.90oB,  reducing sugars from 2.33 to 2.88%, non-reducing sugars from 6.27 to 6.83%, total sugars from 9.24 to 10.05% and TSS/acid ratio between 14.47 to 23.90. The coefficient of variability varied 11.07 to 31.55% for titratable acidity, 0.00 to 23.02% for TSS, 5.24  to 18.53% for reducing sugars, 2.09 to 15.14% for non-reducing sugars, 0.00 to 6.80% for total sugars and 18.23 to 156.51% for TSS/acid ratio in these crosses.

ABSTRACT

Immature zygotic embryos were isolated from field grown papaya fruits harvested at 90-105 d post-anthesis. The embryos were placed on half-strength Murashige Skoog (MS) medium supplemented with 2, 4-D (2.0 mg l^-1) and cultured under dark for a period of 2 months. The frequency of callus formation was around 66%. After 2 months, calli were transferred onto a half-strength MS basal medium and cultured for 3 months with frequent subculturing (once in 3 wk) on the same medium. The frequency of somatic embryo formation was around 36.6%. The somatic embryos on MS medium supplemented with BAP (0.4 mg l^-1) and NAA (0.02 mg l^-1) germinated and produced shoots. The emerging shoots of 2-3 cm height with 2-3 trilobed leaves were placed onto an MS basal liquid medium with IBA (1.0 mg l^-1) for root induction. The plants developed with shoots and roots were planted in a pot-mixture (sand:soil:FYM) containing VAM.

Vegetables are short duration crops and provide more returns per unit area and time besides, they play a major role in balancing human diet, thus, helping in alleviating hunger and malnutrition (Annon., 2009). In the previous few years, India has attained quantum jump in vegetable production and present estimated vegetable production is around 162.9 mt in an area of about 9.4 mha (NHB, 2014), and holds second position at global level. But this level of vegetable production is insufficient to meet the recommended daily intake (~300 g per capita per day) for a huge population of the country. This is mainly due to lower average productivity (17.5 t/ha) of the nation. There are several crop diminishing factors such as soil borne- and foliar- diseases, insect-pests and nematodes as biotic factors, and soil or water salinity, alkalinity, metal toxicity and heat and moisture stress as abiotic factors, which greatly affect vegetable crops yield in almost all growing areas, especially in fragile arid and semi arid regions.

Lawn

Lawn is a natural green carpet, and it is important feature of a landscape and a garden without a lawn is not considerd complete. Lawn is an area of land closed mowed grasses it is primarily developed for aesthetic and recreational purpose. A lawn is an integral part of a garden and landscape besides aesthetic and recreational purpose and several other purposes. Lawn provides a place for taking rest after tiring of the day, holding parties, social functions, passive and active recreation. Distinguishing characteristic of turf grasses is the ability to with stand close mowing and still providing a functional dense healthy ground cover. Lawn is also called as heart of garden. Lawn can be called as turf pitch a field, sod, yard, are green depending upon the plantation usage continent. Lawn is a ground cover of perennial grass, ditch, persists enclose mowing and require proper management practice.

J.B. Olcoth designed first experiment in lawn grass at Monchester in USA in the 19th century. Festuca and Agrostis species were used to make turf (Lawn) during 1880 in U.K. In the early 19th century lawn become wild spread out side of parks golf course. Now a day in many metro cities of India lawn has become a cultural of the passion of many homes.

ABSTRACT

Aprotocol has been developed for the induction, maturation and germination of somatic embryos from leaf explants of Hevea brasiliensis (clone RRII 105). Leaf explants were cultured with their adaxial sides on modified Murashige and Skoog (MS) medium supplemented with different combinations of phytohormones such as 2,4-D & BA, NAA & BA as well as 2,4-D, BA and NAA. Compact calli could be developed from the cut ends of the explants on media containing 2,4-D (1.5 mg/l) and BA (1.0 mg/l) whereas pale yellow friable calli was obtained on media which contained NAA (0.2 mg/l) along with 2,4-D (1.2 mg/l) and BA (1.0 mg/l). The calli formed were detached from the explants and sub cultured for proliferation in medium containing reduced auxin (0.4 mg/l 2,4-D) and slightly increased level of sucrose (40 g/l). Proliferated calli were then sub cultured for embryo induction in modified MS medium supplemented with different auxins and cytokinins. Embryo induction could be achieved in modified MS medium containing BA (2.0 mg/l), GA (1.0 mg/l) and NAA (0.2 mg/l) and maturation occurred in WPM medium containing BA (0.3 mg/l), TDZ (0.5 mg/l) and GA (1.5mg/l). The cotyledonary stage embryos developed into plantlets on transfer to hormone free ½ MS medium. A distinct feature of this study is the induction of somatic embryogenesis from leaf explants of Hevea which has not been reported previously. This explant could be used further as an ideal target tissue for Agrobacterium mediated genetic transformation in Hevea.

Garden

Garden is a place where vegetables, fruits and ornamental plants are grown mainly for recreation. The garden incorporates both natural and man-made materials.

Types of garden

The major garden types which shall be discussed are: English garden, Mughal garden, Persian garden, Italian garden, French garden, Japanese garden etc. Out of these the Mughal, Persian, Italian and French styles fall in the category of formal gardens, whereas the English and Japanese gardens are classified in the informal style of gardening.

Quite a large number of vegetable crops are grown in the country either on a commercial scale or limited to backyards of homesteads. A few crops have similarity while others have dissimilarity in their climatic and soil requirements, parts used, method of cultivation etc. While describing individual vegetables, there is possibility of repetition in many aspects. In order to avoid repetition, it is essential to classify or group them into different classes/groups. Different methods of classification followed in vegetables are described below:

I. Botanical classification

Botanical classification is based on taxonomical relationship among different vegetables. Plant kingdom is divided into four viz. Thallophyta, Bryophyta, Pteridophyta and Spermatophyta. All vegetables belong to Division Angiospermae of Spermatophyta. It is further divided and two classes viz., Monocotylydoneae and Dicotylydoneae.

Abstract

Lean season ornamentals of Shimla are observed for two years. Out of them, the flowering plants include those blooming more or less throughout the year, those having extended flowering up to early winter i.e. November, those begins to flower well before their actual flowering season i.e. spring and those which yield flowers mostly in winter. Some plants having good stalk length, keeping quality and attractive flower such as Reinwardtia indica, Asclepias curassavica and Spermadictyon suaveolens can be tried as cut flower. Some of foliage plants during spring produce beautiful flowers. Planting of these dual purpose plants will have the advantage of beautifying these temperate hills all the year round. Others are evergreen foliage plants with insignificant flower value. All the above plants belong to various utilitarian groups viz. annuals, herbaceous perennials, trees, climbers etc. and can be put into different uses. Some are potential multipurpose economic plants. This preliminary treatise reveals that there are adequate plants available within the premises of Shimla for lean season beautification. The ways to proceed further are also detailed.

Abstract

Open pollinated seedlings of chrysanthemum offer opportunity of large variations for different attributes, such as seed setting, growth and flowering parameters. The desirable seedlings selected from the germplasm collection of Department revealed that only few cultivars set seeds under Solan, Nauni condition  of Himachal Pradesh. Out of 1368 seedlings raised of different seed setting cultivars, 111 were found promising for various attributes concerning to uses of chrysanthemum.