eChapters

Abstract

Himachal Pradesh is endowed with varied type of climate providing conditions to grow temperate and sub  tropical fruits. Persimmon (Diospyros kaki) appears to be readily adapted to wide range of climatic conditions and offer a good potential for diversification of fruit culture. Though it was introduced in Himachal Pradesh in early nineties by the European missionaries but it could not be established as commercial fruit crop. Persimmon cultivation suffers from lack of organized plantings, inadequate vegetatively propagated plant material, non-availability of suitable cultivars and standardization of various orchard management practices. Besides, problems of poor fruit set, heavy fruit drop, astringent nature and lack of sufficient knowledge regarding fruit maturity and marketing mired its cultivation. The efforts made in recent years to overcome the problems the fruit seems to be on the thresh hold of popularity and with diversification in the fruit culture this fruit has great future.

In last few decades awareness about social and environmental issues has been increased. Agriculture is one of basic enterprise that has major role in these two issues . Organic agriculture is one of the agriculture production system that not only supportive to the environment but also sensitive to the social issues like employment, health, migration etc. Definitions given by two international organizations also verify this concept.

Artificial flowers are a great source of handmade decorations. Beautiful handmade artificial flowers are suitable for banquets, weddings or to decorate any room in the home. They are generally made of paper or cloth and in a variety of colors. They come in either a bouquet form or can be ordered as individual stems. There are different kinds of artificial flowers including silk flower, latex flower, flowers made from natural leaves and embroidery flower.

1. FRUITS

Fruit is a part of a flowering plant that derives from specific tissues of the flower, mainly one or more ovaries.

1.1. Types

1.1.1. Fleshy

1.1.1.1. Simple fleshy fruits

Develop from a flower with a single pistil.

ABSTRACT

Rose (Rosa hybrida Linn.) is universally acclaimed as the ‘Queen of Flowers’. But this woody plant is considered less amenable to in vitro culture as compared to herbaceous plants. This is due to the fact that the establishment rose explants is difficult due to heavy phenolic exudation. In the present study, this problem was solved by a very simple and cost effective method. The cut ends of the explant were sealed with wax after thorough sterilization. Ten different explant collection periods with 20 different hormonal combinations in MS culture medium were taken for establishment. The winter dormancy period (Mid- December) was considered best for explant collection and showed 85% survival. Amongst the hormonal combinations 5mg/l BA coupled with 0.05mg/l NAA was considered the best. The average number of leaves (7.5) and average shoot length (7.3 cm) was also highest in this treatment, 15 days after culture establishment.

The processing operations are carried out based on the principle of physical differences found in a seed lot.

ABSTRACT

Haploids are of great importance in breeding programmes for the production of homozygous diploid lines. This technique reduces the time span in developing new varieties and fixes the traits including recessive ones in the homozygous condition. The haploid embryos or doubled haploid plants could be used in mutation breeding, genetic engineering, biochemical and physiological studies. Isolated microspore culture for the production of haploids has not yet been reported in Hevea brasiliensis. Hence the present work was initiated with the view of producing haploid plants by the culture of isolated pollen grains. The flower bud of the clone RRII 105 at the late uninucleate to early binucleate stage was used for culture initiation. Different pretreatment solutions were tried at different temperatures (4°C, 27°C and 33°C) with the macronutrients of N6 media. Three kinds of callus induction media were used viz. N6, WPM and Hunters medium supplemented with myoinositol (200 mg^-1), glutamate (150 mg^-1), phenyl acetic acid (7.0 mg^-1), sucrose (70 g^-1) and growth regulators. Induction medium include moving liquid, static liquid and semi- solid medium. The ovary co-culture was also tried in the induction medium to increase the frequency of callus formation. Pretreatment of the anther with 0.3 M mannitol at 33°C promoted the division of microspores. Static liquid cultures improved the formation of multicellular microspores compared to semi-solid or moving liquid cultures. N₆ basal medium supplemented with 2.0 mg/l 2.4-D and 1.0 mg/l KIN induced microcalli formation. When the ovary co-culture was included in the callus induction medium, the rate of cell proliferation was increased considerably and the duration of callus formation was reduced from 3 months to 14 days. The results of the present study throws light towards the possibility of regenerating doubled haploids in future.

1. VEGETATIVE PROPAGULE

Vegetative propagation or asexual propagation is the method of propagating plants with the use of organs other than the seed and spore. In contrast to sexual propagation, the union of the male and female sexual gametes (fertilization) is not a requisite to the production of new plants. Hence the word “asexual”, which means “without sex” or “not sexual”.

23.1 Dry Flower Packing

Careful packing is one of the most important aspects of any product. Dry flower packing is the simplest way to capture the essence of summer flowers till long. Dry flowers should be packed nicely to avoid any damage. They should be packed alone in appropriate sized carton loosely cushioned with paper.They should be packed in a manner to keep them away from direct sunlight leaving some room for air. Packing material should be stable and firm to hold the dry flowers well without any damage during storage or transportation. Dry flowers can be packed in a variety of ways: in poly bags, window box, cardboard box, or in a sachet.

9.1 Magnetic seed separator

These machines use a magnetic force to separate damaged seeds or other particles from the good seeds. This is used to solve special problems in seed lots that can not be taken out with our other basic seed cleaning equipment. Most of the time it is used for vegetable and field seeds that have problems in seed damage (fish mouth in cucumber or melon seeds / cracked radish or cabbage seeds), insect damage (beetle holes in cotton seeds) or contamination with soil or plant parts (white plant parts in onion seed) etc.

1. APOMIXIS

Development of seed without fertilization. When the seed formation occurs without sexual fusion, the process is known as apomixis. This occurs by several mechanism, however all apomitic seed have genetic material only from the female plant Apomixis may or may not require pollination and pollen tube germination to initiate seed formation, however sexual union.

ABSTRACT

Ophiorrhiza mungos is a rich source of the potent antineoplastic therapeutic agent Camptothecin, which is effective in the complete remission of lung, breast, uterine and cervical cancer. With increasing demand, most of the plants have been indiscriminately exploited from their natural habitat for the isolation of this valuable therapeutic agent. The biotechnological application such as plant tissue culture seems to be a viable option for the production of this high value therapeutic compound without destroying the natural flora. For this purpose various tissue culture techniques have been established to enhance the production of this compound from different parts of O. mungos. The present study revealed that the Physical elicitation with varying doses of irradiation is significantly higher (0.87mg/ g dry weight) than that obtained by chemical elicitation using methyle jasmonate (0.41mg/ g dry weight) compared to control. Biotic elicitors like chitin, chitosan, fungal and bacterial extracts were also used and considerable increase in the production of Camptothecin was obtained in all these cases. Permeabilization with Tween 20 found to be enhancing the exudation of camptothecin (0.021 mg/ml) compared to control (0.0003mg/ml). Hence, the present study gives an alternative for large scale production of camptothecin without affecting the natural flora in a cost effective method.

1. DEFINITION

Artificial seed technology involves the production of tissue culture derived somatic embryos encased in a protective coating. Artificial seeds have also been often referred to as synthetic seeds. However, the term ‘synthetic seed’ should not be confused with commercial seeds of a synthetic cultivar which is defined as an advanced generation of an open pollinated population composed of a group of selected inbred clones or hybrids. The concept of artificial or synthetic seed is shown in Figure 1.

ABSTRACT

Tinospora cordifolia, a versatile medicinal species with antipyretic and immunomodulatory properties is one of the indigenous sources of the therapeutically valuable alkaloid, berberine. However, the plant contains only meagre amounts of this alkaloid. Hence upgradation of the content of berberine in Tinospora, through biotechnological interventions assumes importance. Four week old leaf, petiole and stem derived calli of two ecotypes of Tinospora viz., Vellanikkara ecotype and Madurai ecotype, subcultured on to Murashige and Skoog (MS) medium, supplemented with various growth regulators, were incubated in light and screened for the presence of berberine employing thin layer chromatography. MS medium with NAA @ 2 mg l^-1 supplemented with BA or kinetin, each at 2 mg l^-1, was identified as the basal production medium for in vitro production of berberine, yielding 7.55 μg and 7.36 μg berberine respectively, per gram of calli. Calli produced from stem segments registered maximum amount of berberine compared to leaf and petiole derived callus cultures. Increasing levels of sucrose in basal medium did not have a favourable influence on the expression of berberine in the test calli. Reducing nitrate and phosphate content of the basal medium to half their strength resulted in enhanced berberine production in in vitro cultures of both the experimental ecotypes. Favourable influence of precursor feeding on upgrading the content of berberine in leaf and stem derived calli of both the ecotypes of the experimental species was also confirmed in the study.

Vertical farming is a system of growing crops in multiple layers in skyscrapers, to maximize the use of land by having a vertical design, whereby plants, animals, fungi and other life forms are cultivated for food, fuel and fibre by artificially stacking them vertically above each other (Despommier, 2009; Kalantari et al., 2017). It is estimated that nine acres in a horizontal farm is equal to 1 acre in a vertical farm. The concept of vertical farming started during the mid 90s by Dr Dickson Despomier, professor of Public and Environmental Health at Columbia University

ABSTRACT

Cassava, sweet potato and yams are root and tuber crops propagated through vegetative parts. The CTCRI is maintaining a large collection of (3985 accessions) these crop germplasm in field gene banks. Studies were conducted to transfer these collections to in vitro storage so as to clean off systemic infection and protect them from loss due to biotic and abiotic stress. Suitable media protocols, method of surface sterilization, inoculation and incubation were developed for these different crop species in order to establish initial cultures, for their multiplication and storage under slow growth. Meristem, shoot buds and tuber sprouts were used as propagules for culture initiation.Murashige and Skoog (1962) medium was found suitable for all the species studied with variations required in the concentration of growth regulators (NAA, BA and GA ) for meristem culture and micropropagation. Slow growth of cultures was induced using osmotic retardants like mannitol and sorbitol. Addenda like silver nitrate and activated charcoal was found effective in reducing leaf chlorosis and preventing leaf shedding, thereby contributing to culture life and regeneration capacity. The slow growth media developed were effective in prolonging the subculture period to 18 months (cassava), 10 months (sweet potato), and 20 months (yams). The studies have helped in establishing 400 accessions of cassava, 272 accessions of sweet potato and 166 accessions of yams in In vitro Active Gene Bank. The results are presented and discussed.

Objective: To study the seed treatment methods and equipments A key factor in achieving the few potential of good quality seed is the blanket treatment of the seeds with agrochemical in order to control or prevent diseases in the  crops.

Abstract

Powdery mildew infected leaves of apple hyperparasitized by Ampelomyces quisqualis were collected from field and examined for the presence of the dark brown pycnidia of  hyperparasite. The hyperparasite was isolated on potato dextrose agar medium by standard isolation technique. The morphological characters of the hyperparasite such as size and shape of pycnidia and pycnidiospores were studied. Six systemic fungicides were evaluated for their compatibility with A. quisqualis under in vitro conditions. Dinocap was least inhibitory giving 65.56 per cent growth inhibition of A. quisqualis followed by mancozeb (66.67%). Effect of these fungicides was also seen on conidial germination and germ tube length of A. quisqualis. Maximum conidial germination and germ tube length was observed in mancozeb followed by benomyl and dinocap.

27.1 INTRODUCTION

In India, the horticulture sector contributes around 29 per cent of the GDP and 37 per cent of the total exports of agricultural commodities from about 13.08% of area. India has been placed as the second largest producer of fruits and vegetables; largest producer and consumer of cashew nut, tea, spices; third largest producer of coconut; fourth largest producer and consumer of rubber and sixth largest producer of coffee in the world. Several new programmes area in operation and significant developments in area expansion, production, value-addition and exports have taken place. Today, as a result of better synergy between research agencies, technological and policy initiatives by the governmental agencies, proactive participation of private players and higher degree of diversification to horticulture, this sector has emerged as a sustainable and viable proposition for even the small and marginal farmers including different stakeholders in the commodity chain. In spite of the remarkable progress made in the horticulture Sector in India, there is lot of scope in improvement in safety and quality aspects of horticultural produce.

The concept of biosafety encompasses a range of measures, policies and procedures for minimizing potential risks likely to appear to the environment and human health. Such safeguards must be put in place now. Biosafety is currently being promoted in a variety of ways by industry, governments and civil society. Quality and safety become prime concern because most of the horticultural produce are consumed daily in fresh, cooked and processed form and contribute major share in our daily diet. To ensure biosafety and quality of horticultural produce we need to ensure: a)Supply of quality inputs, b)Adoption of good cultural practices, c) Handling and processing of horticultural produce in scientific /hygienic manner, d)Packing, storage, transportation and delivery etc. in such a way that it reaches at consumers’ table without deteriorating the quality.

To ensure such aspects various preventive and certification measures have been initiated by government and certification agencies all over the globe including India , such as prevention of Food Adulteration Act 1954, Agricultural Produce Marketing Act, 1937, Food Safety Standards Association of India (FSSAI), Food Safety System Certification (FSSC:2200), International Food Certification (IFC), Food Chain Traceability, Hazard Analysis and Critical Control Point (HACCP), Food Product Orders (FPO), etc. The major emphasis under these certifications/regulations is to ensure quality and safety of the produce and minimize the risks by reducing the biological, chemical, physical hazards like microorganism contamination, level of heavy metal/chemicals/ drugs beyond the critical limits, residual effects of toxic substances etc. Brief of a few certification/regulations are listed below.

Abstract

Amongst various soil-borne diseases of apple, white root rot (Dematophora necatrix) causes severe losses both in nursery as well as in grown up orchards and is a limiting factor in raising healthy nursery of apple. Seedlings are killed within a short period depending upon the age of the host and environmental factors. The present investigations were, therefore, undertaken to study the population dynamics of VAM fungi in rhizosphere soil  of apple plant alone and in combination with green amendments and its role in disease suppression. The study revealed that there was variation in population dynamics of VAM fungi alone and in combination with green amendments. It was observed that apple seedlings grown in VAM inoculated fungi (Glomus sp. and Glomus heterosporum) and green amended   soils had more VAM population and colonization in roots than non-amended (minus crop residue) soils after pathogen inoculation. The population of Glomus sp.(350/100gm soil) was maximum in soybean+ Glomus sp. inoculated soil and it was observed that VAM fungi and crop residues in combination suppresses the disease to maximum extent as compared to their alone effect and only 8.7 per cent disease index was recorded in apple seedlings inoculated with mungbean+ Glomus sp. followed by 16.03% in soybean+ Glomus sp. inoculated seedlings.