eChapters

1. PPV & FR ACT

With the signing of TRIPs agreement of the World Trade Organization (WTO), India has agreed to provide protection to plant varieties. The Government of India, therefore, enacted “ The Protection of Plant Varieties and Farmers Rights Act (PPV&FR Act)” in 2001 and the Regulations for implementing the Act were formulated in 2003.

The Ministry of Agriculture, Government of India, has been identified as the nodal agency for implementing the PPV& FR Act through an Authority known as “The Protection of Plant Varieties and Farmer Rights Authority”. The Authority came into force in November, 2005.

PPV & FR act has 11 chapters, 4 schedules and 97 sections.

Globalization of economies and liberalization of trade has led an urgent need for prioritizing the potential areas for investment in order to earn handsome amount of income. Even the entrepreneurs, small as well as marginal farmers aspire to take advantage of global opportunity. Diversification to horticultural crops has been found to be best option as they make more profit, generate additional employment for rural masses and conserve natural resources. Agriculture plays a vital role in the Indian economy. Over 52 per cent of the rural households depend on agriculture as their principal means of livelihood. But unfortunately Indian farmers in general are small and marginal land holders with poor resources and realizing income mainly from cereal based production system, which is insufficient to improve their living standards. Agriculture, along with fisheries and forestry, accounts for one-third of the nation’s GDP and is its single largest contributor (www.indianbusiness.nic.in). GVA at Basic Prices for 2019-20 from ‘Agriculture, Forestry and Fishing’ sector is estimated to grow by 2.8 per cent as compared to growth of 2.9 per cent in 2018-19. The GVA estimates of this sector are based on 1^st advance estimates of agricultural production during Kharif season of 2019-20 obtained from the Ministry of Agriculture & Farmer Welfare. For Livestock sector, estimates of production of major livestock products (i.e. Milk, Egg, Meat and Wool) obtained from the Department of Animal Husbandry & Dairying and Fish production data obtained from Department of Fisheries have been used. The crops including fruits and vegetables account for about 56 per cent, the livestock products 30 per cent and forestry & fisheries 14 per cent share of GVA in total GVA of ‘Agriculture, Forestry and Fishing’ sector [As per the estimates released by National Statistics Office, Ministry of Statistics and programme Implementation, GoI, 2020]. Agricultural exports constitute a fifth of the total exports of the country. In view of the predominant position of the Agricultural Sector, collection and maintenance of Agricultural Statistics assume great importance. In 2018-19, India achieved a record food grain production of 285.17 million tonnes beating the previous year’s (2017-18) 285.01 million tonnes, according to data provided by Department of Economics and Statistics (DES). Also, agricultural profitability has increased over the last decade with record increases in MSPs (minimum support prices) for agricultural produce for all covered crops.

Seed Sampling

The size and quantity of seed sample required for seed quality testing remains small as compared to the seed lot hence, representative samples are randomly drawn for this purpose. The process of obtaining a uniform and representative sample from a seed lot for seed quality testing is called seed sampling. The samples are prepared in accordance to ISTA rules to ensure that small size sample should represent truly and in the same proportion all constituents of seed lot.

The present investigation entitled “Diagnostic Study of Farmers in context of Price spread analysis, marketing pattern and Potential assessment of agricultural diversification in Chenani Block of Udhampur district” was carried out during the year 2015. The sampling structure, experimental procedures and techniques adopted during the course of investigation have been described in this chapter.

3.1 Locale of study

The present study was conducted in Chenani block of Udhampur district in Jammu and Kashmir state. Mantalai, Kosar, Bupp and Bashath villages were randomly selected.

3.2 Climate and rainfall

The Jammu and Kashmir state is situated between 32⁰.17'–36⁰.58' North latitude and 37⁰.26´–80⁰.30´East longitude. The state is the northern most part of the India is girdled by Tibet to the east, China and Afghanistan to the north, to its west is Pakistan. To its south lies the states of Punjab and Himachal Pradesh and have been divided into three agro climatic divisions’ viz. outer plain and outer hills, middle mountains and Kashmir valley and inner Himalayas (Ladakh). The outer plains and outer hills includes Kathua and Jammu districts, extends up to Shivalik hills in the north. According to NARP (National Agricultural Research Project) classification this state has been divided into four agro-climatic zones: Temperate, Sub-tropical, Intermediate and Cold-Arid zone. In summer hot dry winds from plains of Punjab make it very hot, dust storms are common with occasional rain. Rainfall occurs from July to September. Average rainfall is 1143 mm and temperature shoots up to 46⁰C during May to June. During December to February temperature is between 13.5⁰C to 20⁰C, rainfall is 150 mm and in upper reaches there is snow. In the region of middle mountains and Kashmir valley winters are very cold. There is snowfall during winter and subzero temperature. In valley, winters are long and summers are short but pleasant. Average rainfall is 732 to 854 mm. The maximum temperature hardly ever goes beyond 35⁰C. In region of inner Himalayas, days are hot and there are no clouds in the sky. Winters are very cold. Temperature falls as low as minus 23⁰C. The average rainfall is 976 mm and humidity level is very low.

Physical purity test determines the proportion of pure seed, other crop seed, weed seed, inert matter, moisture and germination components in the seed lot by weight in percentage in accordance to prescribed Seed Standards. It thus, helps in improving the plant stand, raising a pure crop by eliminating other crop seeds, weed seeds and disease free-seeds. Seed must not be sold until the percentage of pure seed, other crop seed, weed seed, and inert matter are in accordance to seed physical purity standards. Physical purity test is required for seed certification or seed law enforcement and it is a pre-requisite for ger- mination test.

ABSTRACT

Chickpea is an important pulse crop of India and most suited to the use of rhizobia for its nitrogen requirement. Mesorhizobium ciceri which interact with the crop are poorly understood. Attempt has been made to select M. ciceri with several beneficial properties. Sixty four isolates of M. ciceri collected from the nodules of several chickpea varieties and mutant lines were screened for their growth properties on nitrogen free medium. Isolates MC59 and MC 18-7 showed normal growth and had ARA values at half level to that of Azotobacter. PCR amplification results suggested the presence of ACC deaminase gene sequence both in Azotobacter and M. ciceri. Invariably all isolates showed the presence of one plasmid in the range of 35 kb to 40 kb size.

ABSTRACT

Bacterial wilt caused by Ralstonia solanacearum is the major constraint for cultivation of tomato in Kerala. Biocontrol of plant pathogens is becoming an important component of integrated disease management. Among the various antagonists tested against R. solanacearum of tomato, fungi were more effective than bacterial and actinomycetes organisms. Among them, Trichoderma virens, T. viride (Ozhalapathy and Vellanikkara) and T. pseudokoningii were the effective ones, recording the maximum antagonism index (AI) value of 6000 under in vitro condition. They inhibited the pathogen completely by the mechanism of both lysis and overgrowth. In pot culture study, T. viride and T. pseudokoningii and commercial Pseudomonas fluorescens gave maximum protection against wilt incidence. Under field condition, T. viride (Ozh), T. pseudokoningii and P. aeruginosa showed the lowest wilt incidence of 50.0, 58.3 and 58.3 respectively against cent per cent incidence in the control plot.

1). Stem Gall
Diagnostic Symptoms
• Symptom appears in the form of tumor-like swellings of leaf veins, leaf stalks, peduncles, stems as well as fruits.
• Infected veins show a swollen hanging appearance to the leaves.
• Initially the tumors are glossy which rupture later on and become rough. They are about 3 mm broad and up to 12.5 mm long.
• Badly affected plants may be killed.
• In the presence of excessive soil moisture, especially under shaded conditions, when the stem fails to harden and remain succulent, the tumors are numerous (Fig-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Ascomycota
Class : Taphrinomycetes
Order : Taphrinales

1.1 Introduction

Over the centuries, farmers experimented and developed pest management practice to minimize the damage caused by pests. Paul Muller (1939) discovered insecticidal properties in DDT, which resulted in ushering the synthetic pesticide era. The period from the late 1940s through to 1960s has been called the ‘Dark Ages’ of pest control (Newsom, 1980). Calendar based pesticides schedules were developed by the scientists and disseminated to farmers by extension workers. During this period other control measures were not even considered for further research by the agriculture scientists. The intensification of agriculture with the excessive and indiscriminate use of synthetic pesticides (DDT) in the 1940s and 1950s resulted in dysfunctional consequences like the resurgence of target pests, the appearance of new pests, and the development of tolerance to individual pesticides in many species (van Emden, 2002). The period was dominated by the belief that the pesticide was the only solution to pest management. In the 1960s itself, a major change in thinking occurred following the realization that pesticides had serious limitations and in several cases produce undesirable consequences (Norris et al., 2002). A solution to the problem caused by indiscriminate use of insecticide began to be developed by the entomologists through integrated control practices (Zodoks and Schein 1979).

1) Botrytis Gray Mould
Diagnostic Symptoms
• Appearance of a gray, fuzzy mold leaves, stems and on flowers of or plants. Also called, “gray mold”.
• Spotting or blight on leaf and flower stem lesions, and dieback.
• Flower parts become necrotic and die(Fig-1).
Etiology
Domain : Eukaryota
Kingdom : Fungi
Division : Ascomycota
Class : Leotiomycetes
Order : Helotiales
Family : Sclerotiniaceae
 

2.1 Introduction

India is the second largest vegetables producing country next to China. Vegetable crops are grown on an area of 7.2 million hectares (Mha) with an annual production of 113.5 million tonnes (mt) in the year 2005-06 (Rai, 2007). The extent of crop losses in vegetable crops variety with the crop type, location, damage potential of the insect pests involved and cropping season. As vegetables are perishable items and are more prone to pest attacks, and at a conservative estimate, insect pests cause about 35-40 percent losses (Sardana et al., 2005). The major vegetables cultivated by farmers were cauliflower (Brassica oleracea var. botrytis L.), cabbage (Brassica oleracea var. capitata L.), and okra (Abelmoschus esculentus L. (Moench)). The chief pests of the cauliflower and cabbage crops were the tobacco caterpillar (Spodoptera litura Fab.), diamondback moth (Plutella xylostella L.), cabbage butterfly (Pieris brassicae L.), cabbage borer (Hellula undalis Fab.) and aphids (Brevicoryne brassicae L.). In the okra crop, the shoot and fruit borer (Earias vitella Fab.), jassids/leafhoppers (Amrasca bigutulla bigutulla Ishida) whiteflies (Bemisia tabaci Gennadius) and fruit borer (Helicoverpa armigera Hübner) were the main pests. Yield losses due to diamondback moth (Plutella xylostella) 17-99 percent, cabbage butterfly (Pieris brassicae) 69 percent, and crucifer leaf webber (Crocidolomia binotalis) 28-51 percent, cabbage borer (Hellula undalis) 30-58 percent in cabbage/cauliflower. In Brinjal, 11-93 percent loss is caused by fruit and shoot borer (Leucinodes orbonalis). In okra, yield losses due to shoot and fruit borer (Earias vitella) is 23-54 percent, jassids/ leafhoppers (Amrasca bigutulla bigutulla) 54-66 percent, whiteflies (Bemisia tabaci) 54 percent and due to fruit borer (Helicoverpa armigera) yield loss is in the tune of 22 percent (Shanker et al. 2008). The management strategy for the insect pests and diseases remain largely confined to pesticides. The overemphasis on the use of chemical pesticides by the vegetable growers leads to the multitude of problems to human health and ecology.

ABSTRACT

Anursery experiment was conducted to study the effect of solarization, selected native antagonists viz., T. viride and T. longibrachiatum and the standard culture of T. harzianum for assesseing the growth enhancement and management of Phytophthora rot in black pepper nursery. The experiment was laid out at College of Horticulture with 20 different treatments and replicated thrice. The sprouting percentage of cuttings in different treatments was worked out at different intervals, which showed significant difference. In general, it was observed that the maximum sprouting percentage was noticed in treatments where solarized potting mixture and biocontrol agents were used. The population of soil microflora viz., fungi, bacteria and actinomycetes in different treatments were also estimated. The study indicated a fluctuation in the population of soil microbes in various treatments at different periods of observation. The observations on height and number of leaves of pepper cuttings in different treatments were recorded. It was noticed that cuttings raised in solarized potting mixture incorporated with native antagonists, especially T. viride, exerted a significant effect in increasing the height of cuttings. With regard to total number of leaves, it was also observed that addition of native antagonists in solarized potting mixture had a positive effect in increasing the leaf production. Thus, the effect of solarization enhanced the growth parameters in crop plants by making available the required nutrients. The increased growth response of plants by soil application of Trichoderma spp. may be due to the production of growth promoting substances by the antagonists. Thus, the nursery experiment revealed that, in general, soil solarization and incorporation of antagonists had a favourable effect in promoting the growth of pepper plants besides checking the Phytophthora rot in black pepper nursery.

Introduction

Agriculture is highly dependent on environment, and it’s very difficult to get favourable climate and other conditions for optimum crop growth and development as per crop need. Open field conditions are having a high direct affect of the prevailing weather conditions on crop growth, and also on population dynamics of viruses, fungus, bacteria, insects, etc. With time crops has adopted themselves to specific seasons, but the fast changing climatic conditions happening across the globe has changed the climatic characteristics of a season. This has resulted in untimely rains, high temperature fluctuations and changes in other abiotic and biotic factors in geographically varied locations where it was not supposed to be earlier based on various geographical factors deciding the climatic conditions of that area. Therefore, there is need to develop suitable technologies to sustain these challenges. How to address these issues, can we manipulate the climatic conditions or can we provide protection to the crops against climatic fluctuations and various other related stresses.

ABSTRACT

Banana is one of the most accepted and versatile fruit crops across the globe and in Kerala, it is the most important economically profitable fruit crop grown, mainly by small and marginal farmers, providing a vital source of income. However, the productivity of this crop is very much reduced due to various diseases of which rhizome rot disease is an important one. In recent years, this disease has become a serious problem to the commercial cultivation of banana in southern states of India like Kerala, Karnataka, and Tamil Nadu.In Kerala the disease incidence is found to be nearly 30% and it will soon become cent percent if unnoticed since the disease is more seen in banana cultivated in paddy fields, and the area under the cultivation of banana in paddy fields in Kerala is rapidly increasing. The disease is caused by a gram negative bacterium, Erwinia carotovora. Developing an integrated management package involving biocontrol methods is the only remedy for managing the disease within the financial limits of the marginal farmers. With this intension a pot culture experiment was conducted to study the inhibitory effect of different biocontrol agents and botanicals in the incidence of the disease after testing their efficacy under in vitro cond itions. The results revealed that the biocont rol agent Pseudomonas flourescens (commercial preparation @ 7g /plant) was found to effective in managing the disease. The co- efficient of infection was found to be13.9% as against the control pot where it was 56.24%. With regard to the growth parameters also, the treatment with this biocontrol agent gave superior results. Thus it was proved that Pseudomonas flourescens (commercial preparation @ 7g /plant) could be successfully utilized in the integrated disease management making it cost effective.

ABSTRACT

To study the antagonistic potential of rhizobacteria against the soil borne pathogen Rhizoctonia solani, soil bacteria were isolated from the rice fields of Agricultural Research Station, Mannuthy and screened against R. solani. Out of the 22 rhizosphere bacteria, 10 were found to be antagonistic to R. solani. Seven belonged to the genera Bacillus, Pseudomonas, Clostridium and Sporosarcina and the rest three being unidentified. The bacterial isolates viz., 2B, 4B, 19B (Bacillus sp.), 28B and 10B (Sporosarcina sp.) showed increase in inhibition on extending the period of incubation. Maximum inhibition was expressed by 2B on the third day of inoculation followed by 4B, 10B (Sporosarcina sp.), 7B (Bacillus sp.) and Pseudomonas fluorescens. The bacterial isolate 4B and 7B (Bacillus sp.) and 10B (Sporosarcina sp.) completely prevented the sclerotial production of R. solani whereas 1B (Clostridium sp.), 2B, 19B (Bacillus sp.) and P. fluorescens delayed the sclerotial production. Pot culture studies also confirmed the superiority of these rhizobacteria in controlling the infection by R. solani. The potentiality of these rhizobacterial antagonists can be exploited for the management of soil borne pathogens like R. solani.

ABSTRACT

Phytophthora pod rot (PPR) incited by Phytophthora palmivora is the most serious disease of cocoa, inflicting heavy crop losses. Since, antagonistic microbes are useful in ecofriendly management of plant diseases, the present study was undertaken to harness the potential of antagonistic epiphytic microflora isolated from cocoa pods for the management of PPR of cocoa. A quantitative estimation of the epiphytic microflora from pods collected from different locations of cocoa gardens of Thrissur district revealed more abundance of bacteria followed by fungi, actinomycetes and fluorescent pseudomonads. The in vitro antagonistic effect of 16 isolates of fungi, 22 isolates of bacteria, five isolates of actinomycetes and two isolates of fluorescent pseudomonads from pod surface against P. palmivora was carried out in comparison with standard cultures of Trichoderma harzianum and Pseudomonas fluorescens. The study revealed that all the epiphytic fungal and bacterial isolates including standard cultures of T. harzianum and P. fluorescens were antagonistic to P. palmivora in varying degrees. Based on the in vitro screening seven promising epiphytic fungal isolates and five bacterial isolates along with standard cultures of T. harzianum and P. fluorescens were evaluated for their efficiency in reducing the infection by P. palmivora on detached pods. The pathogen was inoculated on the pods one hour after application of antagonists. Observations taken 10 days after inoculation of the pathogen revealed that one isolate of T. viride and two isolates of P. fluorescens obtained from pod surface exerted more than 40 per cent efficiency over control in reducing the infection. The standard cultures of fungal and bacterial antagonists recorded 33-35 per cent efficiency. Hence, these efficient epiphytic and standard cultures could be utilized for the management of PPR of cocoa.

ABSTRACT

Interaction of cationic dye acridine orange with lipopolysaccharide (LPS) isolated from Rhizobium strains specific (IC59) and non-specific (TAL1000) to chickpea (Cicer arietinum.L L.) has been investigated by spectrophotometric measurements. The strain specific (IC59) lipopolysaccharide induced decrease in absorbance of the α-band of acridine orange at 490 nm and finally upon further addition of LPS /Dye ratio in the range of 30~60 giving rise to α-polymeric metachromatic band at 660 nm. In contrast the LPS from non-specific strain (TAL1000) induces a decrease in absorbance at the α-band till LPS / Dye ratios are 0~50. This suggest that the anionic changes of strain specific LPS is different from non-specific strain enabling formation of large acridine orange aggregate on the surface of specific strain.

Bioinformatics encompases the use of tools and techniques from three separate disciplines; molecular biology (the source of the data to be analyzed), computer science (supplies the hardware for running analysis and the networks to communicate the results), and the data analysis algorithms, which strictly define bioinformatics. So the list below incorporates events from these areas into a brief history of the field.

ABSTRACT

An in vitro evaluation was carried out to find out the efficiency of bacterial and fungal antagonists in checking the growth of pathogens of Kacholam. Out of the two methods tried to find out the effectiveness of Pseudomonas fluorescens against the two isolates of Ralstonia solanacearum, cross streaking produced lysis at the juncture and point inoculation produced clear inhibition zones. Among the two fungal antagonists tested Trichoderma viride was more efficient than Aspergillus niger because of its faster growth over the pathogen. However, both the antagonists showed complete overgrowth and dense sporulation on the area where the pathogen was streaked. To find out the efficacy of Pseudomonas fluorescens against leaf spot pathogens like Colletotrichum gloeosporioides and C. capsici, streaking on both sides yielded more than 50 per cent reduction in growth of both the fungal pathogens. The fungal antagonist T. viride showed a faster growth over pathogens than A. niger eventhough both recorded an antagonism index of 1500. Dense sporulation was seen over the pathogens in case of T. viride. The ability of fungal antagonists to produce volatiles and their effect on fungal pathogens were also assessed. It was noticed that the volatiles produced by T. viride was more effective than that of A. niger. Even though the extent of inhibition was less compared to direct action, this could also be one of the mechanisms of action of both the antagonists. The study revealed that all the three antagonists were effective against both bacterial and fungal pathogens and they could be utilized under field conditions.

ABSTRACT

Soil samples from pepper growing tracts of six districts of Kerala viz., Ernakulam, Idukki, Kollam, Kottayam, Thrissur and Pathanamthitta were assessed for total microbial population. Thirty two fungi, 17 bacteria and three actinomycetes were isolated and studied for their antagonistic potential against the foot rot pathogen, Phytophthora capsici. All the 32 fungal isolates were found antagonistic to the pathogen and majority of them belonged to the genera Trichoderma, Aspergillus, Penicillium, Fusarium, Pythium, Cunninghamella and Rhizopus. Among these maximum inhibition was recorded by Trichoderma spp. which included T. pseudokoningii, T. longibrachiatum, T. polysporum and T. harzianum. The laboratory studies were followed by field trials laid out in farmers’ fields (newly planted pepper gardens) in six districts of Kerala. Effect of biocontrol agents in wilt incidence revealed that the two-time treatment of combined application of Trichoderma local isolate and potassium phosphate was superior in checking the foot rot incidence. The treatment, Trichoderma local isolate + Bordeaux mixture applied twice came in the second position. Data on biometric observations revealed a significant increase in the number of laterals in plots treated with Trichoderma local antagonist and potassium phosphate as compared to plots treated with Bordeaux mixture and copper oxychloride. Results of quality analysis of berries revealed that oleoresin content ranged from 6.98 - 11.84 per cent and piperine content from 3.61 - 5.92 per cent and the quality remain unchanged due to various treatments. The study has provided an ‘eco and health friendly’ biological control for the management of Phytophthora foot rot, the most devastating disease of pepper.