eChapters

ABSTRACT

Cassava has developed importance mainly as an industrial crop due to its ability to store large quantities of starch (74% to 85% of dry weight) within secondary root structures. Cassava starch is exploited as a source of human food, feed for livestock and as raw material for the food processing and chemical industries. Research accomplishments during the last four decades helped India to have the top position among cassava growing countries with an average yield of 24 t ha-1. However several traits like starch quality, high protein , post harvest deterioration etc can be altered only through the development of transgenics. The present study summarizes the results of the experiments undertaken to optimize the protocol for the development of transgenic cassava through Agrobacterium mediated gene transfer. Friable Embryogenic Callus (FEC) was developed in the cassava var. TMS 60444 us ing Murashige and Skoog (MS) media with picloram (50μM). Experiments were conducted to study the efficiency of different Agrobacterium stains viz, AGL 8, GV3101, C58C1, LBA 4404 and EH105 in transforming FEC. The strains differed in their growth rate. The strains EH105 and AGL were slower in growth as compared to other strains and the protocol was modified accordingly. Among all the strains, EH105 resulted in maximum recovery of transgenic tissues. The paper also summarizes the present status of development of transgenic cassava along with future thrusts.

1. INTRODUCTION

Wild pomegranate (Punica granatum L.) is one of those fruits which has got a great economic importance. The name “pomegranate”is derived from Latin pomum (apple) and granatus (seeded) which has influenced the common name for pomegranate in many languages (like in German Granatapfel, seeded apple). It has widely been cultivated throughout India and drier parts of South-East Asia, Malaysia, the East Indies and tropical Africa. The most important growing countries of pomegranate are are Egypt, China, Afghanistan, Pakistan, Bangladesh, Iran, Iraq, India, Mayamar and Saudi Arabia. In India, it grows in the vast tract of the hill slopes of Jammu and Kashmir and Himachal Pradesh. One of the important centers of wild pomegranate, in Himachal Pradesh is Darlaghat, located about 50 Km from Shimla. The fruit provides substantial income to the farmers particularly where it is found growing wild such as in Mandi, Kullu, Sirmour, Solan, Shimla, and Chamba districts of Himachal Pradesh. It is found very common and gregarious in drier and sub-marginal land of mid hill regions of outer Himalayas at an elevation of 900-1800 m above mean sea level (a.m.s.l). Wild pomegranate is considered as one of the commercial fruits of Himachal Pradesh in the form of dried arils popularly known as “Anardana”. Besides Anardana, other value added products can also be prepared from this fruit as detailed in this chapter.

Dietary constitution of Indian people is mostly cereal based. However, the cereals supply chiefly carbohydrates. Therefore, there is deficiency of protein, minerals and vitamins, especially vitamin A, riboflavin, vitamin C, etc. in the diet. The dieticians advocate the intake of 125 g leafy vegetables, 75 g other vegetables and 100 g root and tuber vegetables (total 300 g) everyday because vegetables provide all the nutrient components like carbohydrates, protein, fat, minerals, vitamins and water along with roughages, which are the essential constituents of a balanced diet.

India has attained food security by increased cereal production due to green revolution. In case of vegetables, although there has been increase in production from 15 million tonnes during 1950 to 94 million tonnes in 2005 but still more vegetables are needed to meet the minimum requirement of at least 250 g per day per capita. There has also been increasing demand for fresh export and for processing industries. Moreover, there is a pressure for increasing vegetable supply caused by constantly increasing population in a world of limited acres.

Addition of new agricultural land is of course not possible. In this situation, plant breeding can be expected to contribute substantially to greater vegetable productivity. This can be accomplished not only by the breeding of basically higher yielding varieties but also by the development of varieties that help to stabilize production through resistance to various biotic and abiotic stresses.

1. INTRODUCTION

Food additives have been used for centuries in food processing practices such as smoking and salting of meat. Prior to the advent of refrigeration, food grown in the summer had to be preserved for the winter. Salt, sugar, and vinegar were commonly used preservatives. Additives serve many roles and their common uses includes maintaining product consistency and palatability, providing leavening or controlling pH, enhancing flavour and imparting colour. A food additive can be defined in many ways. The Codex Alimentarius Commission, which develops international regulatory guidelines for food additives, provides the following definition of a food additive:

"Any substance not normally consumed as a food by itself, and not normally used as a typical ingredient of the food, whether or not it has nutritive value, the intentional addition of which to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation, treatment, packing, packaging, transport or holding of such food results, or may reasonably be expected to result, (directly or indirectly), in it or its by-products becoming a component of or otherwise affecting the characteristics of such food. The term does not include contaminants or substances added to food for maintaining or improving nutritional qualities".

F.A.O and W.H.O in 1956 defines food additives as non-nutritive substances added intentionally to food, generally in small quantities, to improve its appearance, flavour, or storage properties. The definition did not include vitamins and minerals. Food Safety and Standard Authority of India (FASSI) in 2011 defines food additives “as any substances which is added to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation or treatment of such food and affects the characteristics of such food, but does” not include contaminats or any substance(s) added to the food maintaining or improving the nutritional values. Food additives are those added intentionally to foods and are not naturally a part of a food but can be a part of food unintentionally.

7.1 Introduction

In this chapter, process, outcome and impact evaluation of the vegetable integrated pest management programme (IPM) implemented in the subtropical area of the Jammu region of Jammu and Kashmir (J&K) state during 2008-09 and 2009-10 was studied. There are many research designs employed for impact evaluation. These include one group before and after comparison design, one group time-series design, non-equivalent control group design and ex-post facto research design. Social scientists most often used before and after IPM (longitudinal) comparisons, with and without IPM, and control group (latitudinal) comparisons, and a combination of the two (longitudinal and latitudinal). A robust measure of the impact of a programme, however, is the difference-in-differences (Ashenfelter and Card, 1985) quasi-experimental design, a statistical technique used in econometrics and quantitative sociology to determine the difference between the treatment (with) and control group (without) over time. The difference-in-differences quasi-experimental design, also called double difference (DD), used to compare the treatment (with IPM) and control group (without IPM) over time. The objective of our quasi-experimental empirical study was to determine the impact of the vegetable IPM programme on the adoption of non-chemical pest management practices, pesticide use, and field use environmental impact in the sub-tropical region of the J&K state. Difference-in-difference quasi-experimental control group design (with/without, before/after) was used to compare the changes before and after the IPM programme between the IPM trained farmers and the non-IPM farmers. The DD model was employed to identify the impact of the IPM programme on the adoption of non-pesticide IPM practices, pesticide use by weight (a.i.), pesticide use frequency, farmers’ knowledge regarding safe use and handling of the pesticides, and field use environmental impact to address the bias introduced by a non-random sampling procedure as the farmers from FFS were selected intentionally (Table 1). The DD model also eliminated the in-built, systematic or seasonal effects other than the training effect.

Digging and Filling of Pits

1.    Marking of pits and planting should always be done with the help of planting board.

2.    The guide pegs are installed at both the ends.

3.    One meter deep pits of one meter diameter should be dug.

4.    Top 30 cm soil should be kept on one side, which is used for refilling the pits as it is fertile soil.

5.    Bottom 70 cm soil should be kept on other side, which is discarded.

6.    The pits should be left exposed for a few days before actual planting.

7.    These pits should be refilled with mixture of topsoil, 2-3 baskets of silt and 2-3 baskets of well-decomposed farmyard manure.

8.    The refilled pits should be watered a few days before planting the tree. To each pit add carbofuron (25g) for control of white ants.

Digital technologies are transforming horticultural production by enabling data-driven crop management and precise decision-making. This chapter introduces agricultural informatics, sensors, Internet of Things, remote sensing, drones, artificial intelligence, machine learning, geographic information systems, robotics, and precision technologies. These tools can support crop monitoring, disease detection, irrigation scheduling, nutrient management, yield prediction, phenotyping, and resource optimization. AI-based image analysis can identify symptoms and assist in early crop diagnosis, while sensors provide continuous information about soil and plant conditions. The chapter emphasizes the integration of digital data with practical horticultural knowledge to improve productivity, profitability, sustainability, and resilience while reducing unnecessary inputs and improving decision-making.

ABSTRACT

In vitro propagation of Plumbago rosea was attempted. One year old plants were used as the source of explants. Nodal explants were inoculated on half strength MS medium supplemented with 2,4-D 1.0 mg l^-1. Induction of callus was observed three weeks after inoculation. The calli were sub-cultured on half strength MS medium supplemented with BA (0.5, 2.0, 3.0 and 4.0 mg l^-1) and IAA (0.0 and 1.0 mg l^-1). Eight treatments with six replications were tried. Benzyl adenine 2.0 mg l^-1 promoted the proliferation of shoots (23.2). Rooting of micro shoots was obtained on MS medium supplemented with IBA 1.5 mg l^-1. The plantlets were then transferred to pots containing sand/soil mixture (1:1) and kept in a mist chamber. The survival rate was found to be 90 per cent.

1. DISEASES OF MANGO

Mango is considered to be the king of fruit. India is the largest producer and exporter of mango in the world. Mango possess unique nutritional and medicinal qualities apart from being a rich source of vitamins A & C, besides its attractive form and appearance, delicious taste and appetizing flavor, the ripe mango fruit according to nutritional experts is also highly invigorating, fattening, laxative and diuretic. Every part of mango from root to tip is used in a variety of ways. This crop is affected by many fungal, bacterial and other non parasitic diseases.

1. Mango Malformation Disease (MMD)

Mango Malformation Disease is a fungal disease of mango caused by several species of Fusarium, some yet to be described. Mango is the only known host of the disease. The disease spreads on a tree very slowly, but if left unchecked, can severely reduce yields. The main method of spreading MMD to new areas is through infected vegetative planting material. There is no evidence that the disease can spread on fruit or the seeds, or that it affects human health. It usually associated with the bud mite, Aceria mangiferae but the mites have been shown to spread the disease within a tree and not between the trees. Mango malformation, also known as bunchy top, is a very serious threat to the mango industry, particularly in northern India. The Etiology of the disease still remains obscure and diverse claims have been made about its causes, e.g., physiological, viral, fungal, acarological and nutritional.

Importance of fruits in human diet is well recognized since time immemorial. They are important part of a healthy diet as natural sources of energy, essential vitamins, minerals and antioxidants ever since the beginning of humancivilization. Our country is bestowed with varied climatic conditions, which had made it possible to grow diverse group of fruits from its temperate to tropical, to subtropical and even to arid niches. The cultivation of fruit crops is, presently, one of the most important and profitable proposition. Growing fruit crops translate into realization of more yield/ income from a unit area of land than any of the agronomic crops. However, there is need to considerably increase the yield of fruit crops, particularly under changing environmental conditions as under such conditions diseases are getting more prevalent. Therefore by adoption of proper protection practices, yield of fruit crops can be improved.

Plant diseases caused by fungi, bacteria, viruses and phytoplasmas reduce crop yields (Khoury and Makkouk, 2010).Adoption and support for using participatory approaches help to farmers in disease management of fruit crops, reducing costs and improving production efficiency. Fungal, bacterial and viral diseases pose serious threat in the cultivation of fruit crops in India. To reduce the losses caused by plant disease, a complete knowledge of disease management strategies of fruit crops are essential which are as follows:

Introduction
Vegetable crops are highly susceptible to biotic stresses indicated by disease causing micro-organism (fungi, bacteria, virus and phytoplasma). Indiscriminate use of chemicals for the control of diseases, insect pest have
several disadvantages, particularly increased cost of cultivation, residual toxicity in food chain and development of resistance against pathogen. Management of diseases involves use of resistant varieties and integration of cultural and chemical control measures. Of the biotic stresses much of the breeding efforts have been directed towards developing resistance against diseases. In fact, breeding for resistance, has been one of the most important objective of vegetable breeder in the last half of the century. Genes that contribute to pathogen tolerance/resistance can be obtained from local germplasm resources, or through exotic lines, wild species or genera, or lines from other breedingprograms. In the biotechnological approaches for vegetable breeding also,  most of the achievement has been to generate transgenic plants resistance to disease. Often breeding for resistance has assumed greater importance in a number of vegetable crops.
Major Diseases of Tomato
Early blight: The casual organism is Alternaria solani. The symptoms appears as small, black spots with enlarged and concentric rings in a bull’s eye pattern in the center of the leaves. Tissue surrounding the spots may turn yellow. On fruits, dark brown concentric rings are seen that affects the market quality.

1. Damping-off
Pathogen
Pythium aphanidermatum (Edson.) Fitz.
P. debaryanum                  
Rhizoctonia solani Kuhn.
Fusarium spp.
Sclerotium spp.
Symptoms
Damping-off of onion seedlings occurs in two stages:
    (i)    Pre emergence
(ii)    Post emergence
In pre emergence stage, plumules are killed before they reach the soil surface. They may be infected, killed even before the hypocotyls have broken the seed coat.

Introduction

Amaranthus species were grown as the principle grain crop by the Aztecs 5,000 to 7,000 years ago, prior to the disruption ofthe South American civilization by the Spanish Conquistadors. Synonyms such as “mystical grains of the Aztecs,” “super grain of the Aztecs,” and the “golden grain of the Gods” were used to describe the nutritious amaranth grain. Vegetable Amaranthus spp. were and are presently utilized for food from such diverse geographic areas as southwestern United States, China, India, Africa, Nepal, South Pacific Islands, Caribbean, Greece, Italy, and Russia (Stallknecht et. al., 1993). Amaranthus grain is extremely nutritional pseudo-cereal with a higher amount of proteins when compared to true cereals. It is a reasonably well balanced food with functional properties that have been shown to provide medicinal benefits. Amaranth associate underutilized crop and an inexpensive supply of proteins, minerals, vitamin A and C, appears to be a future crop which may substantiate this demand because of its tremendous yield potential and biological process qualities, conjointly recently gained worldwide attention (Topwal, 2019). Amaranth as a unique plant with a long and mysterious history is the obj ect for science and also for business. Its characteristics such as absence of gluten and special composition of oil lead this plant to the position of very important plant for the future. The Advantages of Amaranth for Food Industry: Gluten-free, High-quality protein, Non Food-allergy, Thickening property of amaranth starch. The Advantages of Amaranth oil for Cosmetic Industry: Squalene content has a preserve function for skin and hair against sun and radiation, Available for very sensitive skin, Non- Allergy amaranth oil on skin. Amaranth has a big advantage, because it could be use by two ways-biomass for the bio fuel production and grain for food production (Matusova, 2008). As dyes, the flowers of the ‘Hopi Red Dye’ amaranth were used by the Hopi (a tribe in the western

Introduction

The apple (Malus domestica), a valuable and economically significant crop, is grown in India’s northwestern Himalayan region. After Orange, Banana, and Grape, it is the fourth most widely grown fruit worldwide. The tree is native to Central Asia, where its wild ancestor Malus sieversii is still found today. Apples have been cultivated in Asia and Europe for thousands of years. The world’s largest apple grower is China and in India it is grown in the hills of Uttarakhand, Himachal Pradesh, and Jammu & Kashmir in India. The average summer temperature during the active period is about 21-24°C growing season. Apple does this best in areas where trees are dormant. The color development will be better in winter when plenty of sunlight hits. It is possible even at an altitude of 1500 -2700 meters from sea level. 1000-1250 mm well distributed rainfall during the growing season which is most suitable for optimal growth and fertility of apple trees.

Scab

Ventruia inaequalis (Spilocaea pomi Fr?)

Diagnostic Symptoms

• Symptoms generally noticed on leaves and fruits.

Introduction

The banana (Musa parasidiaca) belongs to the Monocotyledons class, the Zingiberales order, and the Musaceae family. It is indigenous to Southeast Asia. They thrive in tropical and subtropical climates and are widely grown in countries such as India, China, the Philippines, Ecuador, Brazil, Uganda, Colombia, and Indonesia. A banana’s average length is 7 to 9 inches, and its diameter is 2 to 3 inches. Bananas are frequently planted during the rainy or monsoon season, when the soil is moist and the young plants can absorb enough water. In tropical and subtropical climates where they are commonly grown, the planting season typically runs from April to June. Planting may take place in September or October in areas with a dry season, following the rainy season.

Introduction

India is the second largest producer of vegetable in the world after China. Every year diseases cause economic losses (8-23%) and also post-harvest losses (20-25 %) in vegetable crops. Bean (Phaseolus vulgaris L.) is a nutritionally important and remunerative crop grown throughout the world and is consumed both as a vegetable, shelled green beans as well as dry beans. It is known commonly known as french bean, common bean, kidney bean, field bean, snapbean, dry bean etc on the basis of its variety and edible purpose. The crop is infected by a number of diseases. Common bacterial blight, Anthracnose, White mold, Bean Common Mosaic, Bean yellow mosaic, Web Blight, Halo blight, Dry root rot and wilt, Bean Rust are some of the major diseases.

Bacterial Blight of Bean

Three types of bacterial blight are found to occur in beans viz. Common bacterial blight or Bacterial blight, Halo blight and Bacterial brown spot. These three diseases affect leaves, pods, stems and seeds in similar manner such that it is usually impossible to distinguish the diseases from one another based on the visual symptoms. While common blight is usually more prevalent in warmer weather, the other two occur more in cool weather.

Introduction The betel vine, scientifically identified as Piper betle L., belongs to the Piperaceae family, which encompasses plants such as pepper and kava. Paan leaves, widely cultivated in countries like Thailand, Philippines, India, Malaysia, Taiwan, Sri Lanka, and various Southeast Asian nations, are commonly used as a popular mouth freshener after meals. This traditional practice is predominantly observed in South Asia and among specific Asian diaspora communities worldwide, where betel leaves are commonly chewed as part of a betel quid or paan, often combined with areca nut or tobacco.

In the cultural traditions of Odisha, offering a bundle of betel leaves symbolizes respect and marks the beginning of auspicious events. Taxonomically, betelvine falls under the genus Piper in the Division Magnoliophyta, Class Magnoliopsida, Order Piperales, and Family Piperaceae.

Introduction

Brinjal, Solanum melongena L. a solanaceous and the native vegetable of India is very favourite among the Indians. It is an economically important vegetable among the small scale farmers and low income consumers of India. It ensures some assured source of income to the resource poor farmers throughout the year due to long fruiting and harvesting period. Brinjal fruits are of a low calorie value and have a mineral composition that is beneficial for human health; these fruits are rich source of minerals (potassium, calcium, phosphorus, sodium, chlorine, iron etc.) and vitamins and have some medicinal importance (Choudhary, 1967). In India, brinjal is grown on the wide range of soils and other variable environmental conditions. In production and productivity, India stands second in the world after China which is mainly grown in the states like West Bengal, Orissa, Bihar, Gujarat, Maharashtra, Karnataka, Uttar Pradesh and Andhra Pradesh. The world production of brinj al in the year 2016 was estimated to be 51.29 million MT in an area of 1.79 million hectare with productivity of 28.59 MT per ha China, the world leader of brinjal production produced 32.03 million tones of brinjal with a productivity of40.96 Mt per ha in an area of 0.78 million ha. The productivity of brinjal as compared to China is very less (18.90 MT/ha) in India although we are the second largest producer ofthis popular vegetable (FAOSTAT Website (http://faostat3.fao.org/home/E).

Introduction

Brinjal (Solanum melongena), is a self-pollinated plant also known as eggplant, is an easily cultivated plant belonging to the Solanaceae family.Brinjal ishigh productive vegetable and easily grown everywhere.It is also known as poor’s man vegetable. Brinjal is cultivated widelyand grown as a vegetable crop in any environment. Brinjal grown large amount in India, Pakistan, Chinaand United States. The origin of the brinjal is India. In Brinjaleconomic parts are fruit. It is a high nutritional valuable food.The brinjal fruit and whole plant use as a medicine in some area. It is cultivated everypart of India except high attitude, all the year around. Depending upon consumer’s preference and yield, different types of cultivars are cultivated.Somewild spp of brinjal are fond in northeast states of India and Malaysia. Solanum insanum, a wild cultivar of the plant, mainlyobserved in the dry and hilly region of west Bengal.Some yellow color brinjal varieties are found in Malaya.