eChapters

Abstract

Experiments on micro irrigation and fertigation system were conducted under four agronomic conditions viz. pot culture, a young conventionally planted apple experimental block, a high density experimental block and a conventionally planted bearing apple orchard. Direct correlation was observed between growth and micro irrigation treatments of 40% and 60% depletion of soil moisture at field capacity (FC); allowing depletion up to 80% of FC was found to be detrimental causing permanent damage and mortality.  Two doses (low and high applied at 10 weekly intervals) of fertigation were tried and were found at par with each other.  In fact, accumulation of N in soil was observed when applied at high dose after the third application whereas in case of applications at low dose a steady high N level was maintained throughout the growing season.  Observations on physiological parameters revealed better performance by the micro irrigated and fertigated trees.  Powdery mildew in apple could also be affective controlled by pesticide delivery through the fertigation system; however, some pesticides were found to affect the growth of the apple trees.  Fertigation has found to reduce fertilizer application by 95% in apple orchards opening further possibilities of reduced chemical pollution in cultivated soils and agricultural produce.

Introduction

Plant needs nutrients for their growth and development. In a systemic agriculture, nutrients are supplied to the plant in different amount, form and time for the economic and optimum crop production. Green revolution realized in the late sixties of the last century is an outcome of efficient use of improved varieties and nutrient management. Success of the green revolution has increased use of chemical fertilizers in the post green revolution era. Soon the chemical fertilizers become indispensible from all the crops including vegetable and plantation crops. Non judicious use of chemicals in the agriculture has lead to the serious health, soil and environmental hazards. Biofertilizers are considered to be the most safe, promising and economic component of sustainable agriculture. Biofertilizer is a substance which contains living or latent microorganisms which, when applied to seed, plant root or soil, colonizes the rhizosphere or the interior of the plant and promotes growth by providing and essential nutrients or increasing the supply or availability of primary nutrients to the host plant. They can applied in all the agronomic, horticultural and plantation crops.

Abstract

The present study of effect of drip irrigation and rootstocks on apple cvs Red Fuji and scarlet Gala under high density plantation was taken up at Horticultural Research Station, Seobagh, Kullu (HP) during 2002-2004. Six year old plants grafted on clonal rootstocks viz., EMLA-106, EMLA-7 and EMLA-111 planted at 3x3m were taken for studies. Five levels of irrigation at 100% ETC, 80% ETC, 60% ETC, 40% ETC and basin irrigation at 100 ETc levels were applied through drips starting from March to fruit maturity. Each treatment was replicated four times with one tree in each in a split plot design. Fertilizer application and cultural practices were carried out as per the recommendations of the university. The data on yield, fruit quality and water use efficiency were collected and subjected to statistical analysis. The fruit firmness, TSS, titratable acidity, reducing sugars, total sugar and non-reducing sugars content decreased with increase in the volume applied through drip. There is a little difference among the varieties and rootstocks for biochemical parameters. Various physical quality parameters were significantly improved by drip irrigation over basin irrigation. The fruits of both the varieties had maximum length, diameter and weight on the rootstock EMLA-106. Similarly yield was also influenced by various levels of irrigation.  Drip irrigation at 40% ETC resulted in maximum water use efficiency (0.022 to 0.023 kg / plant/liter) in Red Fuji during both the years under study.

ABSTRACT

An in vitro evaluation was carried out to find out the efficiency of bacterial and fungal antagonists in checking the growth of pathogens of Kacholam. Out of the two methods tried to find out the effectiveness of Pseudomonas fluorescens against the two isolates of Ralstonia solanacearum, cross streaking produced lysis at the juncture and point inoculation produced clear inhibition zones. Among the two fungal antagonists tested Trichoderma viride was more efficient than Aspergillus niger because of its faster growth over the pathogen. However, both the antagonists showed complete overgrowth and dense sporulation on the area where the pathogen was streaked. To find out the efficacy of Pseudomonas fluorescens against leaf spot pathogens like Colletotrichum gloeosporioides and C. capsici, streaking on both sides yielded more than 50 per cent reduction in growth of both the fungal pathogens. The fungal antagonist T. viride showed a faster growth over pathogens than A. niger eventhough both recorded an antagonism index of 1500. Dense sporulation was seen over the pathogens in case of T. viride. The ability of fungal antagonists to produce volatiles and their effect on fungal pathogens were also assessed. It was noticed that the volatiles produced by T. viride was more effective than that of A. niger. Even though the extent of inhibition was less compared to direct action, this could also be one of the mechanisms of action of both the antagonists. The study revealed that all the three antagonists were effective against both bacterial and fungal pathogens and they could be utilized under field conditions.

Abstract

European red mite is a  serious pest of apple in Himachal Pradesh. Recent survey has shown that 80 per cent apple orchards are infested with this pest. As a result of continuous feeding fruit remained undersized, poor coloured and sour in taste. New acaricides fenazaquin (0.001%) and propergite (0.057%) proved highly effective Standard acaricides dicofol ( 0.037%) and insecticide endosulfan (0.07%) remained effective upto 15 days only.

ABSTRACT

Phytophthora rot is the most destructive disease of black pepper nursery inflicting heavy crop losses. Considering the seriousness of the disease, the present study was undertaken to isolate and select the efficient antagonists from pepper nurseries raised in Government farms at Chelakkara, Pazhayannur, Mannuthy and Pananchery of Thrissur district. A quantitative estimation of the rhizosphere microflora from different nurseries revealed more abundance of soil bacteria followed by fungi and actinomycetes. Based on cultural characters of the rhizosphere microflora, 22 fungi, 20 bacteria and five actinomycetes were selected for further studies. Antagonistic action of soil microflora against Phytophthora capsici was studied by dual culture method in comparison with that of standard culture of T. harzianum. The study revealed that all fungal isolates and five bacterial isolates were antagonistic towards the pathogen with varying degrees. Among the fungal isolates, 13 isolates including T.harzianum recorded cent per cent inhibition of P.capsici. From among the fungal isolates that showed antagonistic reaction in dual culture, further selection of efficient ones was carried out based on the antagonistic index (AI). The isolate 22 F₂ (Trichoderma sp.) showed the maximum AI of 3000 followed by isolate 34 F₂ (Trichoderma sp.) and T. harzianum with an AI of 1500 each. The cultural and morphological characters of the efficient native isolates (22 F₂ and 34 F) were studied and were identified as Trichoderma longibrachiatum Rifai aggr. and Trichoderma viride Pers. ex S.F. Gray aggr. The three antagonists were found parasitic on P.capsici as evidenced by excessive coiling, penetration and disintegration of the hyphae and thereby these Trichoderma spp. used in the study is found to have effective parasitic effect on the pathogen in addition to some degree of antibiosis and these fungi could be utilised for the management of Phytophthora rot in black pepper nursery.

ABSTRACT

Phytophthora pod rot (PPR) incited by Phytophthora palmivora is the most serious disease of cocoa, inflicting heavy crop losses. Since, antagonistic microbes are useful in ecofriendly management of plant diseases, the present study was undertaken to harness the potential of antagonistic epiphytic microflora isolated from cocoa pods for the management of PPR of cocoa. A quantitative estimation of the epiphytic microflora from pods collected from different locations of cocoa gardens of Thrissur district revealed more abundance of bacteria followed by fungi, actinomycetes and fluorescent pseudomonads. The in vitro antagonistic effect of 16 isolates of fungi, 22 isolates of bacteria, five isolates of actinomycetes and two isolates of fluorescent pseudomonads from pod surface against P. palmivora was carried out in comparison with standard cultures of Trichoderma harzianum and Pseudomonas fluorescens. The study revealed that all the epiphytic fungal and bacterial isolates including standard cultures of T. harzianum and P. fluorescens were antagonistic to P. palmivora in varying degrees. Based on the in vitro screening seven promising epiphytic fungal isolates and five bacterial isolates along with standard cultures of T. harzianum and P. fluorescens were evaluated for their efficiency in reducing the infection by P. palmivora on detached pods. The pathogen was inoculated on the pods one hour after application of antagonists. Observations taken 10 days after inoculation of the pathogen revealed that one isolate of T. viride and two isolates of P. fluorescens obtained from pod surface exerted more than 40 per cent efficiency over control in reducing the infection. The standard cultures of fungal and bacterial antagonists recorded 33-35 per cent efficiency. Hence, these efficient epiphytic and standard cultures could be utilized for the management of PPR of cocoa.

Solanum melongena L. (2n = 2x = 24, family Solanaceae, often cross-pollinated) is known as eggplant in the United States and aubergine in France and England. It is one of the few cultivated solanaceous species originating from the Old World. It is known as brinjal in its home country, India, where it was domesticated long ago and where the greatest diversity exists. Widely grown in temperate and tropical Asian countries, eggplant has been also a common crop in the Middle East and around the Mediterranean basin and is now cultivated worldwide. Although an Old World taxon, S. melongena unexpectedly shares strong genetic similarities with New World Solanum species, namely, tomato and potato. These syntenic features have been discovered and will undoubtedly assist in an unlocking of the genes controlling eggplant key horticultural traits (Daunay, 2008).

As per FAOSTAT website-2018 quoted by Ministry of Agriculture and Farmers Welfare, Government of India, Horticulture Statistics Division (2018), the world production of eggplant was 51.29 million tons from 1.79 million ha with a productivity of 28.65 tons/ha during 2016. Top ten countries in eggplant production during this period were China, India, Egypt, Turkey, Spain, Iran, Indonesia, Italy, Japan, and Philippines. The bulk of eggplant production came from top ranking China (0.78 million ha area, 32.03 million tons production and 41.06 tons/ha productivity) followed by India (0.66 million ha area, 12.60 million tons production and 19.09 tons/ha productivity) occupying the second rank. Productivity-wise, Spain is top rank holder with productivity close to 65 tons. Leading brinjal producing states in India are West Bengal, Odisha, Gujarat, Bihar, Andhra Pradesh, Madhya Pradesh, Maharashtra, Chhattisgarh, Karnataka, Haryana, Jharkhand and Assam.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

Elephant foot yam, also known as suran or jimmikand, is gaining popularity due to its high yield potential and culinary qualities. It is grown in Andhra Pradesh, Bihar, Gujarat, Kerala, and Maharashtra in India, as well as in the north-eastern states of Odisha, Tamilnadu, Uttar Pradesh, and West Bengal. This crop is being embraced for commercial production in northern and eastern India, thanks to the advent of a high yielding, non-acrid variety. Elephant foot yam has an output potential of 50-60 tonnes per hectare. Elephant foot yam is a tropical tuber crop that has been grown for generations in tropical Asia. It is gathered for its edible corms, tubers, and smooth petioles, which all contain important minerals.

Elephant foot yam is an underground stem tuber belonging to the family Araceae, one of the most popular tuber crops, extensively used as a favourite vegetable by millions of people in India. It has both nutritional and medicinal value and is usually consumed as cooked vegetable. It has high dry matter production capability per unit area than most of the other vegetables. Elephant foot yam is a remunerative and profitable stem tuber crop. The crop is gaining popularity due to its shade tolerance, easiness in cultivation, high productivity, less incidence of pests and diseases, steady demand and reasonably good price.

Introduction

Foods deteriorate in quality due to a wide range of reactions that may be physical, chemical or biological. The various forms of spoilage and food poisoning caused by micro-organisms are preventable to a large degree by a number of preservation techniques, most of which act by preventing or slowing microbial growth. These include freezing, chilling, drying, curing, conserving, vacuum packing, modified atmosphere packing, acidifying, fermenting, and adding preservatives. Thermal processes such as blanching, pasteurization or heat sterilization have long been employed in practice as economic, efficient, reliable and safe food preservation methods.

1. INTRODUCTION

The growing demand of consumers for fresh, safe, chemical, additive or preservative free and minimally processed foods has compelled the food scientists and technologists to develop many non-thermal food processing technologies which cause minimum nutritional and sensory qualities impairment in the processed foods. In this endeavour, consumers prefer minimally or non-thermally processed foods rather than the thermally processed one. Today, non-thermal technologies like High Pulse Electric Field (HPEF) processing, High Hydrostatic Pressure (HHP) processing or Hight Pressure Processing (HPP) food irradiation, ultraviolet light, ultra sound, arc discharge, oscillating magnetic fields, light pulses, plasma, chemicals (ozone, carbon dioxide, argon), and the combined methods of these technologies are the major areas of research in the process and food engineering in order to find their suitability for processing of various food products.

Biology, the science of living organisms, is linked with microscopes, test tubes and Petri dishes, but these conventional methods are changing with the advent of Bioinformatics. Due to the advancement in molecular biology and genome sequencing techniques, there is an overflow of biological information. This flood of information has led to a requirement for computerized databases to store and organize the data and to design specialized computational tools to view and analyze the data. Accordingly Bioinformatics can be defined as conceptualizing biology in terms of macromolecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied maths, computer science and statistics) to understand and organize the information associated with these molecules on a large-scale (Tramontano, 2007).

ABSTRACT

The totipotency of triploid endosperm tissue is exploited in many vegetatively propagated crops. Hevea brasiliensis, the most important commercial source of natural rubber, is a highly heterozygous perennial tree with a long breeding cycle. Conventional method of triploid production by crossing a diploid with an artificially induced tetraploid is a lengthy and laborious process. Hence in vitro regeneration of plants from endosperm tissue provides an easy and direct approach for the production of large number of triploids. Hevea fruits at different developmental stages were collected and sterilized using 0.1% HgCl₂ for 15 minutes. Seeds were dissected out and the embryos and inner integuments were removed. Endosperm is cut into small pieces and cultured over MS medium supplemented with various levels of sucrose, phytagel and growth regulators viz BA, Kin thidiazuron, 2,4-D, IBA, NAA, zeatin & GA. Organic supplements viz. yeast extract, casein hydrolysate, coconut milk and banana powder were used for callus and embryo induction. Optimum callus induction was obtained in media supplemented with 2,4-D (2.0 mg ^-1) and Kinetin (3.0 mg^-1). On further subculture of the primary calli to medium containing zeatin (1.0 mg ^-1), embryogenic calli could be obtained. Embryos were induced in MS medium supplemented with ABA (0.3 mg ^-1) , Kin (0.3 mg ^-1) and GA (0.5 mg ^-1). Maturation, germination and bipolar differentiation of the embryos were achieved in MS medium devoid of growth regulators. Root tips from the germinating embryos were subjected to cytological analysis and found to be triploids (3n=54). Regeneration from Hevea endosperm is being reported for the first time. The results indicates that like many other crops, Hevea endosperm responds well under in vitro conditions and endosperm culture could be utilized as a viable technique for triploid plant production.

ABSTRACT

The tomato TYLCV tolerant line MP-1 which has high efficiency of crossability & dwarf mutant dg/dg (Manapal) were choosen for the study of enhanced production of plantlets through tissue culture. The cotyledonary explants, hypocotyl sections & decapitated seedlings were taken to assess the regeneration efficiency of the explants as a base for efficient genetic transformation. The decapitated seedlings regenerated much faster (9.3 days) putting forth new sprouts of shootlets which were then transferred to rooting medium. Whereas cotyledonary leaves showed regeneration of plantlets (18.3 days) which is almost double the duration of decapped seedlings. The hypocotyl sections proliferated into callus & regenerated in a much slower rate (27 days). Hence, the faster rate of regeneration from decapitated seedlings served as a efficient base for enhanced production of plantlets during transformation studies. Also the number of harvest of new sprouts (2.3 regenerations) from decapitated seedlings is an added advantage for deriving more plantlets from single seedlings.

ABSTRACT

Ophiorrhiza mungos is a rich source of the potent antineoplastic therapeutic agent Camptothecin, which is effective in the complete remission of lung, breast, uterine and cervical cancer. With increasing demand, most of the plants have been indiscriminately exploited from their natural habitat for the isolation of this valuable therapeutic agent. The biotechnological application such as plant tissue culture seems to be a viable option for the production of this high value therapeutic compound without destroying the natural flora. For this purpose various tissue culture techniques have been established to enhance the production of this compound from different parts of O. mungos. The present study revealed that the Physical elicitation with varying doses of irradiation is significantly higher (0.87mg/ g dry weight) than that obtained by chemical elicitation using methyle jasmonate (0.41mg/ g dry weight) compared to control. Biotic elicitors like chitin, chitosan, fungal and bacterial extracts were also used and considerable increase in the production of Camptothecin was obtained in all these cases. Permeabilization with Tween 20 found to be enhancing the exudation of camptothecin (0.021 mg/ml) compared to control (0.0003mg/ml). Hence, the present study gives an alternative for large scale production of camptothecin without affecting the natural flora in a cost effective method.

Conventional fertilizers often exhibit low nutrient-use efficiency, resulting in nutrient losses, soil degradation, water contamination and unnecessary production costs. Nano-fertilizers offer controlled and targeted nutrient delivery through formulations engineered at the nanoscale. Their high surface-area-to-volume ratio can improve nutrient availability, plant uptake, growth, bulb quality and storage performance while reducing conventional fertilizer requirements. This chapter explains the nature, modes of action and potential benefits of nano-fertilizers in onion production. It also considers nano-biofertilizers, smart nutrient-delivery systems and nano-sensors. Particular attention is given to manufacturing cost, nanotoxicity, effects on soil microorganisms, farmer accessibility and the need for standardized safety and regulatory frameworks.

Entomology, a Greek word entomoV, entomos, “ that is cut in pieces or engraved/segmented”, hence “insect”; and -logia, logia -study, is the scientific study of insects , a branch of arthropodology. The arthropods made their appearance during the earliest Devonian period while humans appeared only in Quarternary. Insects possess an immense degree of evolutionary maturity as against man, account for more than two-thirds of all known organisms, date back some 400 million years, and have many kinds of interactions with humans and other forms of life on earth. Entomology is a speciality within the field of biology and it is rooted in all human cultures from prehistoric times.

In India, insects were known from Vedic era and records of insects in India were evident from India’s mythological epics, the Ramayana and Mahabharatha. 

The scientific community working in the field of crop protection is experiencing an increasing academic and industrial interest in the discovery and development of new bio-insecticides as environmentally friendly pest control tools to be integrated, in combination or rotation, with chemicals in pest management programmes. In this context, Entomopathogenic nematodes (EPNs) as a bio-control agent have proven successful and are now commercially mass produced in six of the seven continents to treat pest problem in agriculture, horticulture and forest crops. They actively search for the host, including those in cryptic habitat stages of insect pests in soil environment. They can be considered as good candidates for integrated pest management in ustainable agriculture due to plants and mammals which are not adversely affected and have ease of mass production. We present a general overview on the current state of knowledge of EPN and their mutualistically associated bacteria and their utilization as biological control agent against insect pests in world and India are briefly presented in this chapter.