eChapters

Introduction Broccoli (Brassica oleracea L. var. italica) having chromosome number 2n=2x=18 is a native of Mediterranean region. The broccoli was developed in eastern Mediterranean region and Italy is the main diversification which was later introduced into England about 1720. It is a cool season crop which produces fleshy green flower heads arranged on branches sprouting from thick edible stalk in such way that it looks like a miniature bonsai tree. It has the same family as cabbage and cauliflower. Even though it resembles cauliflower by forming head like structure but it has longer flower stalks with loose large green terminal head (10 to 20 cm). In Italy broccoli is called as broccoli which means “the flowering crest of a cabbage.” Due to the presence of bountiful nutritious properties, it is also known as “Crown Jewel of Nutrition”. It is a great source of vitamins A, B and C and minerals like iron, fibre, calcium, zinc, magnesium etc. Apart from the minerals and vitamin it also a rich source of riboflavin, thiamine and niacin.

Abstract

Paradoxically, on one hand wild relatives of crop plants got utilized as donor parents in developing hybrids and on the other hand got lost due to lack of conservation of practices, thus genetic erosion. And temperate fruit genetic resources are no exception. Over dependence of growers/orchardists on modern high yielding cultivars and all round development led to neglect of valuable genetic resources of fruit crops. Yet, there are scores of examples where wild relatives have been exploited for genetic improvement of temperate fruits and nuts and still there exists a rich plethora of genetic diversity in the Himalayas which remains untapped and underutilized. Through the process of naturalization, several  elite genotypes, often referred to as geographical/ecological races and biotypes have emerged possessing unique characteristics valuable from horticultural point of view.

Abstract

India has now become the largest producer of fruits after China by producing 45.2 million tones of fruits from 3.78 million hectare area (NHB, 2004). India is first in the production of mango, banana, sapota and acid lime. India has also recorded highest productivity in grapes, sapota and acid lime in the world. India produces about 40 fruits commercially; however, the major share is from tropical and subtropical fruits like mango (28.17%), banana (29.43%), citrus (15.56 %), guava (3.97%) and grapes (2.76 %). Among temperate fruits apple has maximum area and production and 2. 98 % share in total production. Temperate fruits growing in India spread over some 18 states. These states are categorized into 3 major regions, namely, Northern Western Himalayan Region (NWHR), North Eastern Himalayan Region (NEHR) and other hilly areas.

ABSTRACT

Commercial propagation using tissue culture techniques started in seventies for orchids in USA. Since then, the science and technology of tissue culture have advanced particularly after the development of the most widely used medium by Murashighe and Skoog (1972). The science has led to the development of the fascinating area of gene transfer. Today, micropropagation protocols are available for almost all horticultural crops. But, there are certain grey areas where research need to be intensified to solve the problems confronting certain difficult to propagate species belonging to plantation and spice sectors. The major area that needs to be addressed is recalcitrance.

In general, germination of onion seeds is poor, and, in the case of old seed stocks, this problem is severe. Seeds soon lost viability in the ordinary storage state. However, cold storage conditions significantly increase the viability of seeds.

Coriander, Coriandrum sativum L. is an erect annual herb belongs to the family Apiaceae. Coriander may also be referred to as Cilantro, Chinese parsley or Dhania. In India it is cultivated in Rajasthan, Gujarat, Madhya Pradesh, Tamil Nadu, West Bengal, Uttar Pradesh etc. The leaves of the plant are variable in shape, broadly lobed at the base of the plant. Leaves are slender and feathery higher on the flowering stems.

Nutrients

The nutrients are chemical elements which are absorbed by the plants in more or less quantity to transform light energy into chemical energy and to keep up plant metabolism for the synthesis of organic materials. These materials constitute among other things, foods for humans and animals and a range of raw materials for various industrial uses. Feeding of plants with nutrients is termed as nutrition. Successful growth and production of the plants in general requires a proper supply of the 17 elements. These elements are regarded as essential to life in higher plants.

ABSTRACT

Microbial contamination is perceived as the most serious threat faced by plant tissue culture research laboratories and commercial production units. A culture is often viewed as contaminated when obvious microbial growth incited by fungi, yeast or bacteria is noticed on the culture medium. On the other hand, there can be situations when microbes may be present in a covert or latent form with serious side effects on the cultures. The present investigations were taken up with the objectives of elucidating the cause of poor growth, lack of regeneration response or modification of rooting observed with visibly clean cultures of different horticultural crops. Apparently clean cultures of grape, banana, watermelon, eggplant, gerbera and chrysanthemum of recent origin or after long-term in vitro culturing were used in this study. Indexing the medium of such cultures using fungal/ yeast and bacteriological media brought out the covert association of one or more bacterial types with varying proportions of incidence in such cultures. Indexing of tissue revealed bacterial presence in different parts of in vitro plantlets. In some instances, bacteria showed endophytic colonization while being not detectable in the tissue culture medium subsequent to a disinfection or antibiotic treatment. Such bacterial association appeared to be the cause of in vitro decline observed with long-term micropropagated cultures, lack of reproducibility of tissue culture protocols, failure of in vitro rooting etc. besides being a threat to in vitro gene banks and germplasm exchange.

Frequently referred to as "vegetable confetti," microgreens are produced from a variety of commercially grown food crops, including cereals, vegetables and herbs. They contain completely formed cotyledons that may or may not have partially extended genuine leaves (Treadwell et al., 2020). Seven to twenty-one days after germination, the exact piece of the delicate plant's growing stem is removed, along with the cotyledon leaves and maybe the real leaves (Galieni et al., 2020). Despite their small size, these useful micro veggies offer exceptional sensory qualities, including flavour, texture, scent, look and unusual colours. They normally have a height of 2-8 centimetres.

Pollu beetle: Longitarsus nigripennis:Chrysomelidae

This pest is monophagous to pepper and no alternate host has been reported so far.

Nature of Damage

Adult is a shining blue flea beetle with yellow head and thorax and has thick enlarged femur and tibia. The adult beetle is only the size of a pin head.  The infested berries dry up and turn dark in colour. Berries become hollow and crumble when pressed and such hollow berries are called “Pollu” meaning empty. The eggs are laid on the berries @ 1-2 eggs in each hole. Eggs hatch in 5-8 days. The grub period extends for 30-32 days. The emerging grubs feed on the berries of pepper and consume the internal contents.

In several cucurbits as well as other crops, BER is a physiological condition found (for example, tomato, brinjal and pepper). It typically occurs as a general rot at the flowering end of rising fruit. Usually, the blossom end rot is the result of inadequate or uneven irrigation, high humidity, or other variables that slow the flow of water through the plant. Slow water movement may also result in temporary calcium deficiencies leading to blossom end rot as calcium is added into the plant with the transpiration stream.

This is one of the most serious difficulties in mango farming since it makes mango agriculture less rewarding for farmers. In one year (a year) and very little or no harvest next year, mango trees appear to bear huge crops (off year). This bieniality is therefore also present in normal ranges (Singh, 1990). 

Gladiolus is a genus comprising around 260 species of perennial flowering plants in the iris family (Iridaceae). Commonly known as "sword lilies," the name derives from their long, blade-like leaves—gladius being the Latin word for "sword." These plants grow from underground storage organs called corms and feature tall, unbranched stems that bear vibrant, trumpet-shaped flowers arranged in a single-sided spike. Their striking appearance makes them highly valued both in gardens and as cut flowers.
Most gladiolus species are native to sub-Saharan Africa, particularly South Africa, though some are also found in Europe and Asia. The flowers come in a wide variety of colors, forms, and often display intricate patterns or ruffled edges. Each flower is bisexual and sits between green bracts, with its tepals forming a characteristic funnel-shaped corolla. Known for their bold inflorescences and extended vase life, gladiolus flowers are a popular choice in floral design and landscaping worldwide

There is a high demand for arable land to meet food demand and end malnutrition due to the growth of megacities and the global urban population. To enhance yield per unit area, conventional agricultural practises result in agricultural intensification and land clearing for crop cultivation. It has been established that these behaviours have a negative effect on the ecosystem, including soil and water contamination. It is crucial to take into account the application of vertical farming technology which makes use of both horizontal and vertical space and utilises nutrients, water and time (off-season production with artificial illumination) more efficiently to create a larger yield per unit volume of area than conventional outdoor farming. Since microgreens are rich in phytonutrients and are easily harvested, it is being thought of to grow them utilising cutting-edge vertical farming technology. Forest area being turned over to agriculture causes deforestation, the depletion of natural resources like groundwater, loss of biodiversity due to the destruction of habitats, increased soil erosion and greenhouse gas emissions.

Chrysanthemum (Chrysanthemum spp.), popularly known as “mum” or “guldaudi”, is a major commercial ornamental plant grown for cut flowers, loose flowers, and garden display. Belonging to the family Asteraceae, it is valued for its vibrant colors, diverse flower shapes, and long vase life. Chrysanthemum is attacked by several insect and mite pests, affecting both foliage and floral quality, leading to reduced aesthetic and commercial value.
Distribution: Widely distributed in India wherever chrysanthemum and sunflower are grown.
Biology: The eggs are laid singly on the upper surface of leaves, obliquely inserted into the tissue, with opercula appearing as whitish or brown dots. The incubation period for the eggs lasts about 5 to 7 weeks. Following hatching, the nymphs undergo hemimetabolous development (no true pupal stage). The nymphal period spans 2 to 3 weeks, during which the nymphs moult five times before reaching adulthood. Adult insects are approximately 4 mm long, with blackish bodies and lace-like transparent wings (Rizkawati et al., 2023).

Horticultural plants are grown for their produce like fruit, vegetable, flower, medicinal component, spices etc. Therefore, these plants should be managed in such a way that human desires for the purpose of growing them are fully satisfied in terms of quality and quantity of produce. This demands direct manipulation of plant growth itself or plant environment through various inputs. In manipulation of plant development, training and pruning are important for which our knowledge about plant development and its phenology has to be complete. These practices are important in fruit crops.

ABSTRACT

Recognizing the need to apply plant tissue culture tools for generating planting materials of important spice crops especially vanilla, the Spices Board started Biotech Production Unit at its head quarters building at Kochi with an annual production potential of 8 lakhs plantlets. Complete tissue culture protocols were developed and scaled up for large scale production of vanilla and other spices viz. large cardamom, small cardamom and tree spices. The laboratory is equipped with all modern facilities and is established in an area of about 2000 sft. Department of Biotechnology, Govt. of India also participated in the venture by sanctioning a project entitled “Regional centre for large scale production of tissue culture plantlets of vanilla and other spices” with a financial out lay of Rs 95 lakhs equally shared by DBT, New Delhi and Spices Board, Cochin.

At present there is a stock of over 3.00 lakh vanilla cultures in the lab and already 1.25 lakh plant lets were sent for hardening. The Spices Board, in coll aboration with “Kudumbashree”, a poverty eradication mission of Govt. of Kerala, extend short term training in tissue culture techniques to undergraduates from ‘Below Poverty Line’ (BPL) families and engage them as long term trainees in the Biotech Production Unit. The Board also coordinates and extends support to Kudumbashree members for, establishing and maintaining hardening facilities for TC plantlets thus providing means for empowering women from BPL families.

Ice bank cooling: Storing of fruit and vegetable in storage chamber with positive ventilation and system countries where ice formation is allowed during night time (as electricity cost of night is less as compared to the day time). Melting of ice occurs during day time and with proper air circulation by means of ventilation process, approximately 0.5-1⁰C temperature with 90% RH in the chamber is maintained. Ice Bank Cooler / Air forced cooler leads to desiccation of the produce (Plate 139). So use forced air high humidity cooler and these are known as Ice Bank Cooler. Ice bank coolers are useful for strawberry.

ABSTRACT

Astudy was undertaken to evaluate the performance of tissue culture derived plants vis a vis open pollinated seedlings of cardamom (Elettaria cardamomum Maton). A total of 102.5 ha (units) covering 50 ha in Kerala, 37.5 ha in Karnataka and 15 ha in Tamil Nadu was selected for the present study out of which each demonstration plot (unit) had an area of one hectare in which 80% of the plants are tissue cultured and 20% are open pollinated. Elite cardamom clones were subjected to in vitro multiplication and their respective open pollinated seedlings were compared. The planting was initiated in 1989 and completed with 1991 in all the zones identified. Plant growth characters such as number of tillers, number of bearing tillers and panicles per clump were recorded and compared. The yield was recorded in 1993-94 and 94-95 seasons after its stabilisation. Results indicated that tissue culture plants performed better compared to open pollinated seedlings with regard to growth attributes and yield irrespective of the seasons and zones. In Kerala region tissue culture plants exhibited a yield increase of 24.3 and 36% over open pollinated seedlings for the 1989 and 1990 plantings respectively. Similar trend was observed in Tamil Nadu for 1989 and 1990 planting where the percentage increase in yield became 130 and 21 respectively. In Kerala, the yield performance of tissue culture plants and open pollinated seedlings was on par during 1990 planting but in the subsequent year (1991) tissue culture plants showed 24% increase in yield compared to their respective open pollinated ones. Further, the study enabled to identify cardamom clones suited to in various zones of the cardamom tract. Accordingly 14 clones with a yield potential of over 750 kg/ha under moderate management were identified and this indicated that cardamom clones are highly location specific in their performance.

Shoot and fruit borer: Earias vitella (=E.fabia), E. insulana: Noctuidae

This is a pest on cotton, bhendi, hibiscus, holly hock and other malvaceous vegetables. The damage is due to the larva that bores into tender terminal shoots in the vegetative stage and flower buds;  flowers and young fruits in the fruit formation stage.  The damaged shoot drops, wither and dry up.  The infested fruits have bore holes, are deformed and become unfit for consumption.  Bore holes are plugged with excreta.