eChapters

ABSTRACT

Vanilla planifolia Andrews - the high value, exotic spice crop has recently gained wide acceptance in the southern and northeastern states of the country. Being an orchid, germination of naked embryo is limited and this favours genetic uniformity. An attempt was made to induce genetic variability in Vanilla through biotechnology tools for its exploitation in crop improvement programmes. In vitro embryo culture and mutagenesis were applied for the purpose. Plantlets were multiplied, hardened, planted out and evaluated for morphological, biochemical and molecular markers. Great interclonal variability was observed in the in vitro derived plantlets. The intraclonal variability was very low in tissue culture derived regenerants. Isozyme and RAPD assay confirmed genotypic variation. Six superior clones were identified and distributed to farmers for further evaluation.

Shoot and fruit borer: Leucinodes orbonalis: Pyraustidae

The pest is active throughout year, but more during monsoon period. Heavy losses up to 60% can occur.

The adult is a greyish brown medium sized moth with white and hyaline wings, having triangular brown and red markings on forewing. A crescent shaped mark facing outside at the apical margin is typical for the species. About 150-250 creamy white eggs are laid singly on leaves, tender shoots, flowers and developing fruits. Egg period is 3-5 days and the hatched larva completes its life in 15 days with 5 instars.

Diamond back moth: Plutella xylostella: Plutellidae
 
The adults of this moth are small, greyish brown having pale whitish narrow wings and with inner margins yellow. There are three pale whitish triangular markings on hind margins of each forewing. At rest, when both forewings come together, they form a dorsal median patch of three diamond shaped yellowish white spots that give them their name diamond back moth. Hind wings have a fringe of long hairs.

Lab coat: A loose white coat with deep pockets that is worn in a laboratory or medical office.

Labelling: It means any written, printed or graphic matter that is present on the label, accompanies the food or is displayed near the food, including that for the purpose of promoting its sale or disposal. After packaging, labels are required on the finished products intended for distribution and sale. Labelling can reflect on the quality of the product. It must be clear and informative. Information includes Name of the Manufacturer, Date of Manufacture, Net Weight, Gross Weight, Best before etc. Proper storage is needed for maintenance of the quality. Storage must be done only at recommended temperature, relative humidity, light and air / oxygen and carbon dioxide conditions etc. to maintain the quality of the food product. Label may include tag, brand, and pictorial or descriptive matter, written, printed, embossed, graphic, perforated, stencilled or stamped impressions. It is attached to container, cover, lid, or crown of a food package.

Laboratory: A place equipped for experimental study in a science or for testing and analysis. Broadly, it is a place providing opportunity for experimentation, observation, or practice in a field of study.

Lacquer: It is a colloidal dispersion of a solution of cellulose derivative, resin and plasticizer in solvent and diluents, where in all these constituents dried in air by evaporation of solvents, thus yielding a transparent, hard and water proof film.

ABSTRACT

Vanilla (Vanilla planifolia Andrews) is a high value spice crop having immense potential for cultivation in India as a source for natural vanillin. Though the crop was introduced way back in 1835, its cultivation gained momentum only in the recent past due to various reasons. Lack of sufficient good quality planting materials is one of the constraints for expanding the area under the crop. Conventionally vanilla is propagated by vine cuttings, but this method is inadequate to have large number of planting materials in a short span of time. So, micropropagation was attempted and proved to be highly successful. In the present study, performance of vanilla plants raised from tissue culture plantlets and vegetative cuttings was assessed on large-scale in planters’ field covering a total area of 111 ha in Kerala(68ha), Karnataka(28ha) and Tamilnadu(15ha) with the financial assistance of the Department of Biotechnology, New Delhi under the project entitled “Tissue culture Vanilla : Product Plan“. Disease free elite accessions of vanilla identified by the Indian Cardamom Research Institute (Spices Board), Myladumpara from its germplasm repository were used as mother plant source for tissue culture multiplication and also for raising the nursery for producing vegetative cuttings. The field planting for the demonstration plots was carried out during 1996-1999 on a cluster approach basis having 400 plants in each unit or plot of 0.25 ha. Accordingly a total of 444 evaluation plots were laid out under the project covering the aforesaid three states. Out of the 400 plants in a plot, 80% were nursery hardened grown up tissue culture plants and the balance( 20% )vegetative cuttings of comparable length. Observations on growth and yield attributes such as vine length, number of leaves per vine, internodal length, number of beans per vine, bean size, yield etc. revealed that performance of tissue culture plants is on par with that of conventional plants raised from vegetative cuttings of comparable length. It is also observed that if good management practices are adopted, tissue culture plants perform better at the full bearing stage of the plant. This proves that tissue culture plants of vanilla can be popularized as a cost effective and faster source of planting materials compared to conventional vegetative cuttings. As the demonstration plots were distributed under varied agroclimatic situations, the influence of different weather parameters on yield was also ascertained. Further, the project could convince the farmers about the comparable performance of tissue culture plants and that is quite evident from the ever increasing request for tissue culture vanilla plants received by the Spices Board.

Chilli thrips: Scirtothrips dorsalis, Thrips palmi: Thripidae

The adults of this insect are small brownish black with fringed wings. The nymphs are slender and yellowish straw coloured, later acquiring more brownish hue. They lacerate the tissues and feed on the exuding sap. Leaves become crinkled, curled upward and shed. The attack can be confused with chilli mosaic symptom.  In capsicum and chilli, they also feed beneath calyx of flowers and fruits causing flower/fruit drop and reduction in yield and market value of capsicum. Buds become brittle and drop down. Plants get stunted and bronzed. The fruits if at all formed are crinkled and small.

Abstract

Genetic analysis of yield and yield components in tomato (Lycopersicon esculentum Mill.) was worked out by using half diallel mating design. The good general combiners observed were Solan Vajr, FT-5 and UHF-265 for plant height; UHF-107 and UHF-260 for days to first picking; Solan Vajr for number of fruits per cluster; FT-5, EC-191536 and UHF-120 for average fruit weight; Solan Vajr, UHF-265 and FT-5 for harvest duration and FT-5 and Solan Vajr for marketable yield per plant. The best three specific combiners for marketable yield per plant were EC-191536 x Solan Vajr, BL-342-1 x FT-5 and UHF-107 x EC-191536. Hayman’s approach revealed non-allelic interaction in the expression of number of fruits per cluster, number of fruits per plant and marketable yield per plant. Preponderance of non-additive gene action was observed with dominance towards tallness, delayed first picking, more fruits per cluster, longer harvest duration and higher marketable yield, while involvement of complimentary epistasis was indicated in the inheritance of average fruit weight and number of fruits per plant.

Sweet gourd, (Momordica cochinchinensis) (Cucurbitaceae),  called as ‘spiny gourd’,  ‘crow cucumber’, ‘spike fruited crow cucumber’ or ‘elephant bitter gourd’ and ‘gac fruit’ is a wild vegetable, native to East and South East Asia comprising India, Myanmar, Philippines, Vietnam, Thailand, Japan, China, Indonesia and New Guinea (de Wilde and Duyfjes, 2002). It is by far the most extensively distributed and more variable species among the genus Momordica L. Even though, it is restricted to Andaman Islands and Assam in India, it occurs as a natural component of coastal scrub jungles and partially opened forests especially stream banks in South, Middle and North Andaman Islands (Joseph 2005). It is cultivated in Bihar, Jharkhand, Orissa, West Bengal and Assam by tribal people in their homegardens. It is grown as a vegetable in China and Japan (Jeffrey 2001). It is known by various vernacular names as ‘Adavi kakrol’ (Telugu), ‘Hathiya kankad’, ‘Gol kakra’ (Bengali) and ‘Jangli Kakrol’ (Andaman Islands).

Teasle gourd, Momordica subangulata Blume ssp. renigera (G. Don) de Wilde (Cucurbitaceae), is a semidomesticate or a wild vegetable native to Assam-Myanmar region. As in  other dioecious Momordica species, it’s taxonomy and nomenclature were confused with M. dioica Roxb. (spine gourd=kartoli) and M. cochinchinensis (Lour.) Spreng. (sweet gourd=kakrol) (Joseph et al.2006). It is known by many vernacular names like kaksa (Bihar), lamkarote (Manipur), meeta chotela (Sikkim), kakrol (Bengal, Tripura), kakrul (Mizoram), bhat karela (Assam) and gantola (Bangalore). Allied to M. dioica, M. cochinchinensis and M. sahyadrica Joseph and Antony, in general morphology it is distinct from these species in its branched tuberous roots, cordate dentate unlobed leaves, prominently networked leaf veins, large creamy flowers with bull’s eye mark in three inner petals (also in M. cochinchinensis), reniform male bracts, very long female flower stalks and medium sized softly echinate dome shaped fruits with remnant ridges (Fig.1). A few workers reported it as tetraploid form of M. dioica (Misra and Sahoo 1983). It is referred to as M. cochinchinensis by Patnaik and Patnaik (1976), Shadeque and Baruah (1984) and Ram et al. (2002) and M. dioica by Maurya (1976) and Ali et al. (1991), whereas a recent revision of the genus in Malaysia and SE Asia by De Wilde and Duyfjes (2002) proved its botanical identity as Momordica subangulata ssp. renigera  (G. Don) de Wilde.

Vegetable crops: A seed is borne on fruit and fruit is a mature ovary and ovary is a typical part of flower. Flower in angiosperm, consists of four parts these are calyx/sepal, corolla/petals, androecium/stamens and gynocium/pistil, outer whorl of flower is collectively referred to as calyx. The individual components of calyx are called as sepal. Petals are collectively referred to as corolla. Petal is a unit of second inner whorl after sepals. Stamen is part of flower bearing the male reproductive parts composed of anther and filaments. Pistil is part of flower bearing the female reproductive parts composed of stigma style and ovary.

Seed Production Techniques in Vegetable Crops

Share (%) of F₁ hybrids- Cabbage 85%, Watermelon 70%, Okra 23%, Tomato 80%, Chilli 80%, Cauliflower 50%. Among the Asian countries, India is the 2^nd largest production of hand pollinated hybrid seed in vegetables.

Ordinarily, fruit bears a single crown, but fruit bears more than one in some cases. The top of the fruit will therefore be smooth and wide and the fruit will not be ideal for canning. These fruits have an insipid taste and are corky. It is intended to be an inherited character, often found in the Cayenne group to which the Kew variety belongs.

Abstract

Bioinformatics, an integrated science of biology and informatics is a repository of new emerging dimensions like proteome, genome, drug discovery etc, whose concepts have varied applications in various sciences/areas including agricultural/horticultural sciences. Bioinformatics tools are employed to investigate interactions between different biological systems i.e. gene-gene, protein-protein, gene-protein interactions and this information has tremenduous application in agricultural/horticultural sciences. It is the computer aided  data handling approach to understand the life process precisely. Biological problem require innovative database prospective for gene sequencing (management and alignment), recognition of genes and regulatory elements, protein structure prediction, evolution and phylogenetic studies, etc.

Objective Methods: Objective Methods are used for evaluation of food quality. These are based on recognized standard scientific tests to any sample of the product without regard to its previous history. They represent the modern idea in quality control (QC) because the human element has been excluded. This method divided into three groups like physical, chemical and microbiological. The devices are used in this evaluation.

Obligate anaerobes: These microbes do no grow in the presence of oxygen.

Obligate parasite: Obligate parasites is host specific and grows on living host only and attach limited range of host and are very difficult or impossible to grow on artificial culture/media.

Obligate thermophile: These microorganisms grow at a temperature between 38-80^oC.

Oenology: Treatments used to prevent spoilage of wine is called oenology. It is synonym to that of enology.

The planning of a garden is an art. It is said that a poet is born, and in that sense a garden architect is also born. But that does not mean that a garden architect has nothing to learn. On the contrary he should learn enough of geology, geography, garden history, styles of gardening, and above all should have a profound knowledge about plants. It is imperative to know at the outset what is meant by landscape gardening. A landscape may be defined as any area, either big or small, on which it is possible or desirable to mould view or a design, whereas landscape gardening may be described as the application of garden forms, methods, and materials with a view to improving the landscape. The art of designing is known as “landscape architecture,” although the older term landscape gardening” is also popular.

Before discussing the principles of gardening in detail, it will be worthwhile to make some useful suggestions. The first and the foremost thing are not to imitate another garden which has secured a prize in a competition. One has to develop one’s own design giving due consideration to the local conditions. One more mistake which is commonly made is to plant many more specimens than a garden can accommodate causing overcrowding. In a landscape garden any difference in levels has to be taken advantage of, but in a perfectly flat land it will be costly to create artificial undulations. In each garden the care should be at least one feature or there be a second these two should harmonize with each other. Before planning a design are must be sure for what purpose the garden is utility or beauty or both.

World Scenario

The world climatic conditions are divided into Tropical; Sub-Tropical and Temperate climates. Fruits and Vegetables suited to these climatic conditions are grown successfully. Considering the nutritional and economic importance of fruits and vegetables, there is tremendous increase in area and production of fruits and vegetables throughout the world. The present global scenario of fruit and vegetable crops (areas harvested, production and productivity (yield) has been given in Table 1. It shows that the fruits 865.88 million tones has been harvested from 65.24 million Ha land with productivity 13.27 t/ha (Table 1) where as vegetables produced 1075.20 million tonnes from area 57.00 million ha with yield 18.86 tonnes/ha (Table 1) during 2016.

Radish (Raphanus sativus) is a cool weather crop and its origin is in Mediterranean, rapid growing annual or biennial plant belongs to family Brassicaceae, grown for its edible taproot. Its edible roots are having different color from as white to red. Radish is generally grown as an annual plant, surviving only one growing season. It is crop of tropical and temperate region. It is growing as herb in West Bengal, Bihar, Uttar Pradesh, Karnataka, Punjab and Assam areas are major radish growing states. Radish is good source of vitamin B6, Calcium, copper, magnesium and riboflavin. Also it is rich in ascorbic acid, folic acid and potassium.

Alternaria Blight

Economic Importance

Alternaria leaf spot diseases occur in every part of the world, in both vegetable crops and seed crops and most important disease of Brassica species, often results in severe yield losses. It is considered as a number one disease of radish affecting both fresh vegetable and seed production. The disease is more severe when the plants are left for seed production in the field and causes significant reduction in seed yield. Alternaria black spot of radish; black pod blotch of radish. A. japonica is a seed-borne pathogen of plants in the Brassicaceae. A. japonica is known to affect most parts of infected plants depending upon growth stage. There are reports of it on the hypocotyls and cotyledons of seedlings and on the leaves, petioles, stem, inflorescence, fruits (siliquae) and seeds of adult plants. A. japonica occurs in all parts of radish (Raphanus sativus) seed. Its presence in the embryo can result in seed death, but in milder infections it will affect seedlings after germination, causing seedling blight both before and after emergence.

Strobilanthes (of Acanthaceae) is a morphologically diverse and species rich genus extensively distributed in South and Southeast Asia (Carine,1997 and Moylan et al 2004). Approximately 74 species are found along the Western Ghats of Southern India - Sri Lanka biodiversity hotspot (Carine, 1997). The mass flowering of Strobilanthes with blue coloured flower makes them promising plants for gardens.  The genus is subjected to several systematic revisions recently (Bennett and Scotland 2003, Carine et al. 2000, 2004 Carine and Scotland 1998, 2000, 2002 and Venu 2007). The most distinguishing character of the genera (Strobilanthes) is its monocarpic nature, i.e., flowers after a long period of vegetative growth ranging 1-15 years and in a few cases flowers after 35 years. Bremekamp (1944) used the term ‘plietesials’ for such monocarpic plants that take several years to reach maturity before flowering.

India has witnessed tremendous increase in the production of horticultural crops especially fruits since independence. Due to specialized scientific interventions and application of latest research tools, the country is now among the top fruit producing countries of the world ranking second next only to China. However, the productivity has still remained low as against area under the fruit cultivation. Several neglecting issues related to the production technology have remained unattended so far. The old and senile orchards are now reverting towards a declining trend of production because of plant age factor, non-compatible varieties and poor canopy management (Baba et al., 2011).

In the recent past declining productivity of old and dense orchards existing in abundance has become a matter of serious concern for the orchardists, traders as well as scientists. In India 30-35 per cent area under fruit crops is occupied by old, dense and diseased orchards. For overcoming the problem of unproductive and uneconomic orchards existing in abundance, large scale uprooting and replacement with new plantations (rehabilitation) will be a long term and expensive strategy. Therefore research efforts were initiated to standardize a technology for restoring the production potential of existing plantations by a technique called Rejuvenation. The term ‘Rejuvenation’ means renewal or making new or young again. As applied to the orchard tree it would mean restoring the productive capacity of the fruit trees. The meaning of ‘Rejuvenation’ according to Chamber’s dictionary is ‘to recover youth character or to grow again’. Obviously, this would apply to those plants which have attained a stage where they are no more profitable from the grower’s point of view.

The agroclimatic conditions of Indian Himalayan region are suitable for growing temperate fruit crops like apple, pear, peach, plum, apricot, almond, walnut, pecan nut, kiwi, strawberry, hazelnuts, pistachio nuts and other small fruits. The region is extended over the states of Jammu and Kashmir (J & K), Himachal Pradesh (H.P.)  and Uttaranchal (U.A.) in the north-western Himalayan region and Arunachal Pradesh, Sikkim, hilly areas of Assam, Nagaland, Mizoram, Meghalaya and West Bengal in the north -eastern Himalayan region. Other areas are ooty, Kodai Kanal and Nilgiri hills in Tamil Nadu. Mount Abu in Rajasthan and hilly areas of Punjab. Although, temperate fruit growing areas in India do not fall in the temperate zone, yet the prevailing temperate climatic conditions due to high altitude and nearness to snow covered Himalayas has made the temperate fruit cultivation possible.

Annuals

Annuals or seassonals are the group of plants which complete their life cycle in one season or in one year. Annuals are commonly known as seasonal as they complete the process of life like germination, growth flowering and seed formation in one season and finally the plant without. Annuals are widely used for garden decoration, cut flower and pot plants. They are frequently grown as bedding plant and in rock garden on the sides of lily pool and in shrubberies.