eChapters

Plant genetic resources are the most valuable and essential basic raw material to meet the current and future need of crop improvement programme. A wider genetic base assume the priority in plant breeding research aimed as developing new varieties for increased crop production. PGR for food and agriculture are a reservoir of genetic adoptability, which act as buffer against environmental changes and economical challenges. The erosion of these resources results in a severe threat to the world long term food securities. The fundamental objective of the genetic resource conservation is the maintenance of genetic diversity within each of the species. In general PGR include all species which contribute to people by providing food, medicine, feed, fiber etc. PGR is our heritage which needs conservation for prosperity.

Genetic resources management is a complex process starting from the identification of a target gene pool for conservation to the use of genetic resources. Many of these activities not only generate but also require geo-referenced data (Spatial data). Analysis of these data can lead to a greater understanding of the eco-geographic representation of existing collections, increasing the efficiency of conserving and managing genetic resources and broadening our understanding of the distribution of genetic diversity. Genetic resource databases could potentially serve as an information resource to a broader scientific community, providing relatively independent global data sets to meet the unique needs of researcher. GIS can merge genetic diversity information with other georeferenced data such as population density, climate, topography and soils, adding value to genetic resources. GIS analysis can help develop conservation strategies, monitor genetic diversity, select potential collecting sites, design in situ reserves and enhance genetic resources use. Currently, GIS use is constrained by limited georeferenced information.

Pigeon pea seeds discovered in historical tombs suggest it was farmed in Egypt circa 2000 BC. Vavilov (1939) concluded that India is the primary origin of the cultivated pigeon pea due to the wide diversity of germplasm available. It’s a crucial location in rain-fed agriculture. It is grown on 4.92 million hectares worldwide, including 3.58 million hectares (72.7 percent) in India alone. Although the crop is planted in 22 nations, it is only cultivated in considerable areas in a handful of them. Its seeds have become a widespread food in Asia, Africa, and Latin America since its domestication in the Indian subcontinent at least 3,500 years ago. It is an important source of protein for the Indian subcontinent’s people and is consumed on a big scale in South Asia. India is a major producer of pigeon peas. Kenya, Malawi, Uganda, Mozambique, and Tanzania are major producers of pigeon pea on the African continent.

7.1. Status Vegetables are generally sensitive to environmental extremes, and thus high temperatures and limited soil moisture are the major causes of low yields as they greatly affect several physiological and biochemical processes like reduced photo-synthetic activity, altered metabolism and enzymatic activity, thermal injury to the tissues, reduced pollination and fruit set etc., which will be further magnified by climate change. The consequences of the climate change badly hit the vegetable production. Under changing climatic situations crop failures, shortage of yields, reduction in quality and increasing pest and disease problems are common and they render the vegetable cultivation unprofitable. This ultimately questions the availability of nutrient source in human diet.

Introduction

Organic seeds are produced by purchasing conventional non-treated seeds and growing them on organic plot / farm for one season without the use of any synthetic chemical. The seeds harvested are then sold on to organic farmers as organic seeds.

Over 98% of sprayed insecticides and 95% of herbicides reach a destination other than their target species, because they are sprayed or spread across entire agricultural fields. Runoff can carry pesticides into aquatic environments while wind can carry them to other fields, grazing areas, human settlements and undeveloped areas, potentially affecting other species. Other problems emerge from poor production, transport and storage practices.

Production of organic seeds without the use of pesticides like herbicides (weeds), insecticides (insects), fungicides (fungi), nematocides (nematodes), and rodenticides (vertebrate poisons) is a matter of skill and expertise. For this purpose, the seeds used for planting, the soil where they are planted, the water used for irrigation and even the air, need to be kept free of pesticides in order to prevent their contamination.

Seed

Chironji or Charoli (Buchanania lanzan Spreng.) is an important fruit tree of agro forestry and social forestry. In the wasteland development and dryland horticulture, it assumes great significance due to its multifarious uses and capacity to withstand adverse environmental conditions. At present, it is growing under natural forest as an underexploited fruit and gives monitory reward to the tribal community of the country and seems to be boon for them. It is valuable tree species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982).

Removal of seeds from dry fruits is known as threshing while that of wet fruits is known as extraction. Threshing involves beating or rubbing the plant material to detach the seed from its pod or fruit. The detached seed is then winnowed to remove chaff, straw and other light material from the seed.

Threshing / extraction methods

1. Mechanical threshing

• Various types of threshing machines with adjustable cylinder speeds are available for extraction of vegetable seeds.
• Care must be taken to avoid damage to the seed during mechanical threshing, by properly adjusting the speed of the beaters, the width of the gap between the beaters and the concave, the airflow and the sieve sizes.

Salvadora the  “Grape of the Desert” and commonly known as Pilu or jaal is a popular  multipurpose tree (MPTS) found growing luxuriantly in Thar desert and Kutchh region of Gujarat. It is an extremely drought hardy and salinity tolerant plant, capable of growing in hostile weather conditions. Salvadora provides life and support to the people living in fragile ecosystem of Thar desert and hence can be rated as a life line tree. The two important species of Salvadora i.e. Salvadora oleoides Decne and Salvadora persica L. are known to exist in India and known by different vernacular names. S. oleoides is called jaal, Mitha jal (Rajasthani); Pilu (Sanskrit); Bhapilu (Hindi); Kankhina (Marathi); Pil (Punjabi) and Kalawa (Tamil) while S. persica is known as Pilu (Sanskrit), tooth brush tree (English); Chotapilu (Bengali); darakht-I-miswak (Persian); Pilu (Marathi); Varagogu (Telgu) Ughaiputtai (Tamil); goni-mara (Karnataka). Salvadora is native of India, East Africa and Arabia. It is found in wild form in extensive areas of Africa particularly, Turkama, Sambura and Marsabit districts of Northern Kenya, Ferli in North Senegal, South Sivani in Egypt and N. E. Tanzania. Salvadora is also of common occurrence in South coastal areas of Pakistan, Sindh and Baluchistan, Saudi Arabia and other middle eastern countries.

Introduction

For agricultural applications as well as for research work, often we need to prepare solutions of different agrochemicals of varying strength, as per the requirements. Agrochemicals are used to help crop plants grow, produce, and protect them from biotic/abiotic stresses. Many of the agrochemicals (insecticides, herbicides, fungicides, rodenticides, molluscicides, nematicides, etc.) are used to protect the crop from insect pests and diseases. However, some of them (fertilizers and soil conditioners) are used to augment crop yield. While in the previous chapter (Chapter 8) we discussed about preparation of laboratory solutions, in this chapter we will briefly discuss about the preparation of different agrochemicals.

Agrochemical/agrichemical is a generalized term used for the chemicals applied in agriculture. In general, it also includes the feed-supplements used in f isheries and animal husbandry.

Agrochemicals

Fertilizers

Fertilizers are used to augment growth of crop plants and to mitigate nutrient deficiency in the soil. Fertilisers can be organic (containing natural substances, prepared through natural processes) and inorganic fertilisers (synthetic fertilizers, manufactured artificially using chemical processes using natural deposits, by chemical alterations).

Soil Conditioners

Soil conditioners include manure, livestock and leaf compost, peat that help making the soil in good condition. They are laid on the top soil and then mixed properly. They not only increase nutrient contents in the soil but also improve aeration and water holding capacity.

Seed processing is specialized activity and also a tool to improve seed quality further to an acceptable standard. Seed quality parameters like germination, vigour or genetic purity can be improved using processing. Say a seed lot having a minimum standard of 90% germination, but the competition in the market offering seed quality 92 or 95 % then to meet the market demand we can process the seed lots using re-milling or re-conditioning techniques. Also sometimes simple techniques like liquid density separation which uses specific density of the seed to separate good seed from inferior quality can be considered. Various case studies on processing to improve seed quality are given here.

Mahua (Bassia latifolia Roxb.), is a deciduous, hard woody multipurpose tree species (Venkatesh,1998). It is very hardy and thrives well on rocky, gravelly red soils and also on saline and sodic soils. It can grow even in pockets of soil between crevices of barren rock. Trees are grown on degraded rocky area including salt-affected soils and forests area.  Mahua prefers tropical and sub-tropical climate. It can withstand drought admirably. A characteristic tree of the dry region is found in north and central India.  It commonly grows in eastern Uttar Pradesh, Madhya Pradesh, Maharashtra, Bihar, Odisha, Andhra Pradesh and Gujarat.  In Rajasthan, it is also found growing on the wastelands particularly in southern part of the state.  The tree is very well known to rural folk since ages in India.

Seed certification

It is a legally sanctioned system for quality control and seed multiplication and production. It involves field inspection, pre and post control tests and seed quality tests. As per Indian Seed Act seed certification is voluntary and it is not compulsory. The seed that is sold in the market is of two types certified seed or truthfully labelled seed. Whereas truthfully labelled seed is one which is being produced and marketed by the producing company by maintaining the labelling standards.

Marula nut (Sclerocarya birrea subsp. caffra) is a highly valued tree in most parts of Southern Africa because of its medicinal and nutritional properties. The marula tree is adapted to the semi-arid conditions that characterize most parts of sub-Saharan Africa and renders them unsuitable for conventional crop agriculture (Moyo Mack, 2009). The unique nutritional properties of marula and its high tolerance to dry conditions provide opportunities for its development into a plantation crop. On the other hand, the demand for marula plant parts, mainly the bark and roots as medicinal remedies, poses a great threat to wild populations. In the long term, the growing demand of marula products in the food, pharmaceutical and cosmetic industries will not be sustainable from wild populations alone. A rich genetic diversity in Sclerocarya birrea has  reported by Muco et al.

Seed sampling

Seed sampling is to draw a portion of seed from seed lot that represents the entire seed lot. Seed lot is a uniformly blended quantity of seed either in bag or in bulk.

1. Introduction

Medicinal plants are rich source of novel drugs that forms the ingredients in traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates, bioactive principles and lead compounds in synthetic drugs (Ncube, 2008).  In recent years, antibiotic resistance has become a global concern. This drives the need to screen medicinal plants for novel bioactive compounds as they are biodegradable, safe and have fewer side effects (Prusti et al., 2008). Higher plants, as a source for new potential drugs is still largely unexplored and screening of active agents helps to design the lead molecule later in drug development.

Aegle marmelos(L.) Corr., belongs to the family Rutaceae, and is popularly known as Vilvam tree (Tamil) (Gamble, 1935; Mathew, 1983). It is an indigenous tree found in Iran, India, Myanmar, Pakistan, Bangladesh and most of Southeast Asian countries (Singh, 2009).  A. marmelos has been used from time immemorial in traditional systems of medicine for relieving diarrhoea, dysentery, stomachic, digestive, astringent, peptic ulcer and respiratory infection (Nadkarni, 2000). The fruit, leaves, bark and root are edible and possess medicinal values. The unripe dried fruit used to be astringent, digestive and stomachic, the ripe fruit is aromatic, cooling and is used as laxative.

Inventory management system is a computer based system for creating work orders at a processing plant, tracking inventory levels in the go downs, , sales and seed movement out of the processing plant. These modern systems avoid book maintenance and save lots of space. It will also tool for avoiding overstock or delay in movement of seed material for packaging. In most of the processing plants SAP is the most popular inventory management tool which is more efficient than others. SAP is basically a tool of enterprise resource planning which help in material management. The customised software can be used very effectively in a processing plant for the following operations.

Guggul (Commiphora wightii Arnott.) commonly known as guggul, is a much branched shrub yielding gum-resin generally obtained by incision of the bark. Its plant is drought hardy and can  be grown in wasteland,  gravelly, desertic soils of arid parts of the country. This resin, normally referred as guggul, has been shown to have diverse medicinal properties like lowering of lipid activity anti-inflammatory  activity , diuretic, expectorant, diaphoretic  activity, etc (Atal et al.,1975). Its description and uses is mentioned in ancient literatures Ayurvedic treatise Charak (1000 B.C.), Sushruta Samhita (600B.C.). It has  a historical use in  folk medicine and today it ‘s highly commercially important target species .In the last few decades there has been  a sharp  decline in natural population of this species. There is hardly any commercial cultivation of this species. However, at Mangliavas near Ajmer in  Guggul Herbal Farm, more than 15,000 plants have been planted on the rocky site and the material  so  established  is being used for  research purpose on tapping of guggul and other related aspects.

Purity analysis

Purity of a seed lot indicates in percentage how large a fraction is made up of pure seeds of the species in question, and how much is made up of inert matter and other seeds. Impurities may be any non seed material (leaf, flower, fruit fractions, soil etc.), small fractions of seeds of the actual species, as well as seeds of other species. Pure seed may include both dead and empty seed, plus damaged seed, purity does not tell anything about viability. The purity analysis of a seed sample in the seed testing laboratory refers to the determination of the different components of the purity viz., pure seeds, other crop seeds, weed seeds and inert matter. Each component is reported as a percentage of total weight. The objective of the purity analysis is to determine whether the submitted sample conforms to the prescribed physical quality standards with regard to physical components. Large seeds are generally easy to clean and purity analyses are therefore often omitted. However, for smaller seeds contamination with other seeds may occur e.g., during processing.

History of plant breeding
The process of plant breeding takes care of changing or modifying the genetically controlled characters of a particular variety or genotype of a crop. The practice of age-old agriculture uses the process of selection from the existing variability to save a particular quality of seed to grow better crops in the next harvest.
Gregor Mendel who is known as the father of Genetics discovered the law of segregation in the middle of the 1800s. This theory forms the base for the process of plant breeding. The primitive ways used for plant breeding are very different than what modern technology has contributed to the process of plant breeding during the 20th century.
Plant breeding is the science and art of purposeful manipulation of characters of crop species to create new varieties with a set of desired characteristics. Significance of plant breeding has been highlighted especially after the “Green Revolution”.

Senna (Cassia angustifolia Vahl.) popularly called as ‘Sonamukhi’ is widely used medicinal herb in Ayurveda, Unani and also in Allopathic system of medicine. It is well known for its purgative activity (Bentley and Trimen, 1999). The plant is known by various names in common conversation likes Sennai, Sona, Sonamukhi, Sennamukki, Sonpar, etc.  Presently, there is a growing demand of senna  dry leaves and processed products in the national as well as in international markets. In recent years, its cultivation has gained momentum in arid and semi- arid parts of Rajasthan and Gujarat due to its diversified adaptability. It has also been widely adopted by the farmers because of its low cost of cultivation with yield stability. Its main characteristics viz., drought hardy, perenniality, non-palatability to animals has also attracted the farmers for its popularity. Moreover, the senna crop is eco-friendly as it can be utilized for wasteland, sand dune stabilization and desert development (Bohra and Sankhla, 1997; Tripathi, 1999).

Henna (Lawsonia inermis L.) is a multipurpose shrub also known as mehandi. It is grown through out the country as a hedge plant. In India, it was once grown extensively in Punjab and Gujarat. However, gradually major cultivation has shifted to the arid fringes of Rajasthan with the Pali district having the maximum area under henna cultivation. It is taken as an annual ratoon crop under rain fed conditions. Presently there are over 22,000 ha of henna plantations in the Pali district nearly 70 percent occurring in the Sojat area which produces around 20000 tonnes dry leaves worth Rs.40 crores annually. Sojat city is world famous for its mehandi or henna (Heena) powder.