eChapters

Abstract

About 20 percent of the world’s total population is residing in the mountains and this proportion is likely to increase in the present century as environmental degradation and pollution are driving the human population for settlement in the clean and serene environment of the mountains. Himalayan region has sizeable area in countries like India, China/Tibet, Nepal, Pakistan, Afghanistan, Bhutan, etc. Remarkable progress has been made in greenhouse research and development in many countries. However, Himalayan regions which reserve unique flora and fauna have attracted little attention so far. Changing climate, fast increasing population and shrinking land and water resources are the major challenges before us to meet the food and nutritional security of the population at global level. Improvement in the production and productivity of crops are the immediate need to boost the availability of food and improve the economic conditions of the farmers.

Greenhouse technology has tremendous potential to improve production and productivity of crops even under uncongenial soil and climatic conditions. The farmers having very small farm holdings in countries like Japan and South Korea have improved their income and living standards by adopting protected cultivation technologies. Similarly, Israel has demonstrated successful crop production under desert and uncongenial climatic conditions by improved water management practices and using greenhouse technology. However, India is yet to trap the potentials of the technology. Modern greenhouses require uninterrupted huge electric power for control of environment (heating/cooling/air circulation etc), which is considered one of the major causes for global warming. High installation and running (energy) cost, high cost of production; poor government support and lack of farmer friendly /cost effective greenhouse technology are some other challenges. Appropriate modifications suiting to our requirements, as per the need of crop, climate and farmers may lead to popularization of greenhouses in the country, especially among the small and marginal farmers. Defence Research & Development Organisation (DRDO) successfully demonstrated the innovative approach to popularize low cost zero energy based solar greenhouse technology in higher Himalayan regions of the country. Such greenhouses are specifically designed using local resources and also trap natural solar radiation/ heat and maintain it for longer period. Similarly, other approaches like using mud/ unbaked bricks, double wall approach, making hot beds, blackening of walls, using water bottles, greenhouse-cum-animal shed, etc. effective to reduce the adverse effects of the environment on crops with little inputs in hilly regions. Need of the hour is to review our approach for the greenhouse technology in farmers’ prospective and device simple and cost effective innovations for fast propagation of greenhouses in the country. This will not only improve the economic status of the farmers but also provide nutritive food to their families and enhance production and productivity of various crops in the country without deteriorating the environment.

Introduction

Indian subcontinent is one of the twelve mega diversity centres for cultivated plants and their wild relatives. More than 15000 species of flowering plants are indigenous to this region, which include 160 species of economic importance, 320 species of wild ancestral forms and approximately 800 species of ethnobotanical origin. Global diversity in vegetable crops is estimated around 400, of which 80 originated in India. These indigenous vegetables have immense potential in meeting the vegetable requirement of the country. They are rich in nutritional value especially in proteins, minerals and vitamins. Many of these vegetables have medicinal uses. Most of them are not used to their potential and their popularisation is needed in meeting the vegetable requirement of the country in coming days.

Ash gourd (Benincasa hispida Cong.)

Ash gourd also known as Wax gourd (Benincasa hispida Cong.) belonging to family Cucurbitaceae, is cultivated for its immature and mature fruits used as vegetable and in confectionery and ayurvedic medicines. A small fruited medicinal ash gourd is also grown in Kerala which is good for people suffering from nervousness. In areas where winter is mild, the crop can be grown throughout the year. The released varieties are KAU Local, Indu, Co.1, Co.2, APAU Shakthi, Karikumbala, Boodikumbala and IVAG -502. In addition, MAH-1 and MAH-2 are F1 hybrids released from private sector. The crop is raised by sowing seeds in pits at a spacing of 2.5 × 2.0 m in Tamil Nadu, 3.4 × 1.8-2.5 m in West Bengal and 4.5 × 2.0 m in Kerala. The seeds can also be sown in channels at a spacing of 2 × 0.5m. The crop is trailed on branches and twigs spread on the ground. The fruits are harvested at immature or fully ripe stage depending on demand. Immature fruits can be harvested 21 days after anthesis. Mature fruits for storage, long distance transport and seed extraction are harvested after full development of waxy coating on fruit surface. The average yield are 30-35 t/ha.

The Indian region is one of the world’s eight centres of crop plant origin and diversity, distinguished by Russian Scientist N.I. Vavilov. The region is home to a rich endemic assemblage of plants. Scrub jungles, grasslands, dry and moist deciduous forests, semi-evergreen and evergreen forests are the variety of vegetations along the Ghats. The complex terrain and heavy rainfall have helped to preserve flora and fauna in this region. The Western Ghats are an area of exceptional biological diversity and conservation interest, and are “one of the major Tropical Evergreen Forest Regions in India”.  This region is extremely rich in floristic diversity and most of the species are not exploited so far. There are many underutilized endemic flowering plants found in Western Ghats having ornamental value. 

Flora of Western Ghats
 The Western Ghats cover only 5% of India’s total geographical area but contains more than 27% (4000 species) of  country’s total plant species (Nayar, 1996). The Western Ghats, also known as the Sahyadris, extend about 1600 km. starting in North from Tapti river and going down to Kannyakumari in the South. These ranges run parallel to West coast hardly 40 kilometres away on an average from shoreline. The Western Ghats run through states of Maharastra, Goa, Karnataka, Tamilnadu and Kerala. Elevation of Western Ghats is in  900 – 1500 m and in some places goes beyond 2000 m. The Western Ghats form major watershed in Peninsular India and  58 major Peninsular Indian rivers originate from them.

After attaining a rich experience over a period of time, our forefathers have developed very pertinent primitive knowledge in a wide variety of fields (Rohilla et al., 1999). These location specific wisdom are known as indigenous traditional knowleges (ITKs). Indigenous traditional knowledge are the upshots of the pragmatic approach by our sons of the soil. As such, there are many ITKs emerged due to the incremental and cumulative learning among societies living in close connection with nature. The traditional communities are often called as indigenous or local or ethinic people. They have accumulated lots of empirical knowledge gained from the day-to-day life. The variations in availability of raw materials and environmental conditions clubbed with time tested traditional knowledge and wisdom, have made people of different regions of the North Eastern hill states to formulate, develop and perpetuate consumption of a wide range of traditional foods and beverages unique to its places since ages (Savitri and Bhalla, 2007).

Introduction

More and more people are turning to indoor gardening as a fun and functional way to experience nature without leaving the house. A wide variety of plants may be cared for in the comfort of one’s own home by using this method. Indoor gardening may be adjusted to fit any space, whether it a little flat or a large mansion. Improving air quality, adding a supply of fresh herbs, veggies, or colourful flowers, and increasing interior aesthetics are just a few of the many advantages. Regardless of the season or environment, indoor gardeners are able to produce a broad range of plant species thanks to the comfort of regulated temperature and lighting. In addition, tending to houseplants may be a calming hobby that pays dividends in the form of a feeling of achievement and peace of mind.

ABSTRACT

Holostemma ada-kodien K. Schum, is a laticiferous climber belonging to the family Asclepiadaceae. The root tubers of the plant are medicinally important and are utilized as a major ingredient of the drug, Jivanti. The roots of Holostemma are sweet, ophthalmic, emmolient, alterant, tonic stimulant, aphrodisiac, expectorant and galactagogue and are useful in ophthalmopathy, orchitis, cough, burning sensation, stomachalgia, fever and to cure ‘tridosha’ (Warrier et al., 1995). The sugars and amino acids present in the tuberous roots are responsible for the medicinal properties (Ramiah et al., 1981). There is a huge demand for the root tubers of Holostemma in the South Indian pharmacies and more than 150 metric tons of roots are needed every year (Nair et al., 1992). The destructive and ruthless collection of tuberous roots in recent times has led to acute scarcity of the plant and consequently it has been listed as vulnerable and rare in the FRLHT red list of medicinal plants (FRLHT, 1997). Extending the commercial cultivation of Holostemma is practically impossible due to the increasing land pressure and the perennial nature of the crop. An alternative to meet the increasing demand is to produce the active chemicals in Holostemma through in vitro techniques. In vitro techniques have been extensively studied to produce the different secondary metabolites from various medicinal plants. A strong correlation between secondary metabolite production and morphological differentiation gives more impetus to the application of organized cell culture particularly the root cultures for the large-scale production of phytochemicals in vitro. Transformed root cultures - the so called hairy root cultures obtained after the insertion of T-DNA from the root inducing (Ri) plasmid of the Agrobacterium rhizogenes into the plant genome (Ackermann, 1977) has emerged as an important tool for the production of secondary metabolites in vitro (Rhodes et al., 1987). Hairy roots have advantages of fast growth, organ differentiation and stable secondary metabolite production (Banerjee et al., 1995). We report here the hairy root induction from shoot buds and seedling hypocotyls in infection with A. rhizogenes useful in exploring the possibilities of large-scale in vitro secondary metabolite production in this rare medicinal plant.

ABSTRACT

Trichoderma spp. are important group of fungi which are mycoparasitic on several plant pathogenic fungi. Trichoderma spp. are, therefore, used as commercial biofungicides. Genetic manipulation through induced mutagenesis and molecular cloning are two important tools in improving the biocontrol potential of Trichoderma spp. In the present study, attempts were made to induce genetic variability in Trichoderma virens using UV- induced mutagenesis. In the process, a simple protocol was developed for obtaining high frequency mutations in Trichoderma virens. Using a UV-crosslinker, four stable mutants of T. virens have been developed, which are distinctly different from the wild type in appearance and in antagonism against Rhizoctonia solani and Sclerotium rolfsii. One of the mutants, designated as TI is very slow in growth, but sporulates densely, and retained its mycoparasitic potential against R. solani. Though all the mutants were able to antagonize R. solani, all of them were overgrown by the test pathogen S. rolfsii. These mutants could be used to identify genes involved in growth and conidiation in T. virens.

ABSTRACT

Fruit rot and die back caused by Colletotrichum capsici is the destructive disease in chillies. The potential of Pseudomonas fluorescens for the management of fruit rot of chillies was evaluated under greenhouse and field conditions. Among the various strains of P. fluorescens tested in vitro, the strain Pf1 significantly inhibtied the mycelial growth of C. capsici. Combined application of talc-based powder formulation of P. fluorescens to seed (10 g/kg seed) and foliage (1 kg/ha) effectively controlled the fruit rot disease. Early and high induction of various defense compounds were observed due to P. fluorescens treatment.

ABSTRACT

Vanilla planifolia Andrews - the high value, exotic spice crop has recently gained wide acceptance in the southern and northeastern states of the country. Being an orchid, germination of naked embryo is limited and this favours genetic uniformity. An attempt was made to induce genetic variability in Vanilla through biotechnology tools for its exploitation in crop improvement programmes. In vitro embryo culture and mutagenesis were applied for the purpose. Plantlets were multiplied, hardened, planted out and evaluated for morphological, biochemical and molecular markers. Great interclonal variability was observed in the in vitro derived plantlets. The intraclonal variability was very low in tissue culture derived regenerants. Isozyme and RAPD assay confirmed genotypic variation. Six superior clones were identified and distributed to farmers for further evaluation.

ABSTRACT

Microbial antagonists not only reduce the soil borne disease, but also influence the growth of crop plants. In a study conducted for five years on the effect of microbial antagonists on damping off incidence and biometric characters of solanaceous seedlings, it was observed that seed treatment and soil application of Trichoderma viride, T. harzianum, Pseudomonas fluorescens, Azotobacter chroococcum reduced the damping off incidence considerably in brinjal, chilli and tomato. Application of these microbes also increased the per cent germination and promoted vigour of solanaceous seedlings as compared to control. Among the various antagonists, T. viride was the most effective one reducing the damping off diseases as well as promoting the growth of solanaceous vegetables.

Abstract

Apple productivity of Himachal Pradesh with effect from 1995 to 2004 varied from 6.99 t/ha to 0.82 t/ha.  Climatic variables such as effective chill units (ECU) and growing degree hours (GDHoC) were estimated at different stages of dormancy and their relationships with productivity was studied. The duration of rest period had negative correlation (r = -0.716) indicating that the increase in the rest period duration decreased the productivity in the corresponding year.  ECU accumulations during peak winters had significant positive correlation (r =+0.642).  However, the GDHoC accumulations during rest period  (r = -0.679) and peak winters (r = -0.771) had significant negative correlation. During the period of rest completion to full bloom, the GDHoC accumulations showed significant positive correlation with productivity (r =+0.645). Total snowfall recorded during winters was also significantly correlated. However, no significant correlations were observed with respect to rains and productivity of apple

Development can be defined as the process and timing of differentiation of the component events causing qualitative changes in form and function of the plant and therefore, manifestation of economic plant parts. Sowing is the beginning of the process, after which plants emerge, grow and develop with or without transplanting and may be harvested in vegetative (e.g., cole, bulb, tuber, root, salad crops) or reproductive phase (solanaceous fruits, peas and beans, cucurbits, okra, etc.). Growth and development of plants is dependent on abiotic (physical) and biotic (biological) factors. Abiotic factors include the physical environmental conditions and biotic factors include animals, insects, and diseases. Each plant has certain environmental requirements. To attain the highest potential yields a crop must be grown in an environment that meets these requirements. A crop can be grown with minimal adjustments if it is well matched with its climate or growing condition. Unfavourable environmental conditions can produce a stress on plants resulting in lower yield. In such cases the environment can be artificially modified, as done in protected structures like poly house, shade net house, etc. to meet the crop requirements.

Different abiotic environmental factors viz., light, temperature, water and soil greatly influence plant growth and geographic distribution. These factors determine the suitability of a crop for a particular location, cropping pattern, management practices, and levels of inputs needed. A crop performs best and is least costly to produce if it is grown under the most favourable environmental conditions. To maximize the production of any crop, it is important to understand how these environmental factors affect plant growth and development.

Abstract

Different orchard floor management practices had a significant effect on percent soil moisture content at a soil depth of 0-22.5 cm and 22.5-45 cm and soil temperature at a depth of 22.5 cm and 45 cm. Black polythene mulch recorded significantly higher soil moisture content than other treatments at both depths while clean cultivation recorded the minimum soil moisture content throughout the studies. Similar to the soil moisture content, black polythene mulch recorded the maximum soil temperature while hay mulch recorded the minimum soil temperature.

The term ‘Phyto-hormone’ was coined by Thimann in 1948. Phyto-hormones are organic compounds, which are produced naturally in plants, synthesised in one part and usually translocated to the other part where in every small quantity affect the growth and other physiological function of the plants. Auxin was the first hormone to be discovered in plants. At one time, auxin was considered to be the only naturally occurring plant growth hormone however, four other classes of phyto-hormone viz., gibberellins, cytokinin, ethylene and abscisic acid was later discovered. Plant growth regulators include both five specified classes of phyto-hormones (auxin, gibberellins, cytokinin, ethylene and abscisic acid) and other synthetic organic compounds (for example, polyethylene glycol, dinoseb-2-sec butyl 4,6dinitrophenol, proteins and/or amino acids, carboxylic, phenolic acid, humic acids, etc.) having properties of bio-stimulants or bio-inhibitors. Plant growth regulators (PGRs) modify plant physiological processes either by stimulating or inhibiting specific enzymes or enzyme systems to help regulate plant metabolism and thereby enhance yield and product quality. They normally are active at very low concentrations in plants.

Importance of PGRs was first recognized in the 1930s. Since then, wide array of natural and synthetic compounds that alter function, shape and size of crop plants have been discovered. Of the many uses of PGRs, effects on yield are often indirect. A wide range of beneficial effects of PGRs on crop growth and yields are: 1. promotion of germination and/or emergence, 2. stimulation of root growth, 3. promotion, mobilization and translocation of nutrients within plants, 4. modification of flowering, 5. increase in stress tolerance and improvement of water relations in plants, 6. promotion of early maturity, 7. increase in disease resistance, 8. retardation of senescence and 9. improvement of crop yields and/or quality.

ABSTRACT

To study the influence of season on explant collection, axillary buds from mature trees of three elite genotypes of tamarind viz., Sour pulped type, Red pulped type, and Sweet pulped type (Thailand) were collected monthwise from September, 2002 to August, 2003, sterilized properly and cultured in MS medium supplemented with BAP (1, 1.5, 2, 2.5 and 3 mgl^-1) + GA 0.5 mgl^-1 + NAA 0.1 mgl^-1. The studies revealed that, the bud break percentage was highly dependent on the season of explant collection. In case of Sour pulped type, bud break was recorded during the month of April, May and June. Maximum per cent bud break (50.00 per cent) was recorded in May and June but least number of days were required for bud break (12.33) in April month. In Red pulped type, the bud break was observed during February to June. The axillary buds collected and cultured during the month of February recorded the best results with respect to the per cent bud break (100 per cent) and least number of days taken for bud break (7.12 days). In Sweet pulped (Thailand) type, bud break was recorded during the months of February to June. The axillary buds collected and cultured in the month of may recorded the best results with respect to the percent bud break (87.50 per cent) but March showed least number of days for bud break. Nodal segments cultured on MS + BAP 2 mgl^-1 + GA 0.5 mgl^-1 + NAA 0.5 mgl^-1 gave maximum and early bud break in three genotypes, but sprouted shoots showed abscission of leaflets and died later on.

ABSTRACT

Considering the difficulties faced during ex vitro establishment of orchid plants the present investigation was undertaken to elicit information on the influence of different sucrose levels in the in vitro rooting medium and by subjecting the plants to different levels of triazole at the time of planting out in order to overcome the mortality percentage at the time of ex vitro establishment. Among the growth parameters observed viz. crop growth rate, net assimilation rate, and relative growth rate was found to be high in plants treated with 40 g/l of sucrose concentration and also at the triazole level of 5 mg/l. The photosynthetic rates were observed to increase meanwhile the transpiration rate was declined in plants grown under the above treatment levels of sucrose and triazole. The maximum survival percentage was observed when the in vitro plants grown in rooting medium with 40g/l of sucrose which is attributed to the influence of sucrose concentration on morphological characters studied viz. plant height, number of leaves per shoot and number of roots per shoot. Triazole treatment of plantlets at the time of planting out helped in better survival percentage which is the whole sum effect of increase in number of leaves per shoot, number of roots per shoot of plants grown under the triazole level of 5 mg/l. Where as the height of the plant was inversely proportional to triazole concentration,the biometric aspects studied viz root length, total fresh and dry weight of the plants with the above mentioned treatments were found to be superior. The present study envisages the measures to be taken during in vitro propagation and ex vitro establishment of tissue cultured plants in order to over come the field mortality.

Introduction

Despite environmental, bio-physical, and resource constraints in most parts of Rajasthan, even though the state’s geography can be classified into three distinct climatic zones: hot arid, semi-arid, and sub-humid, from a horticultural perspective. These diverse zones have excellent vegetable production potential, a wide range of crop plants, and varied opportunities for resource utilization. However, extremes of high (March–October) and low (December–January) temperature situations associated with abiotic stresses in arid regions restrict the choice of crops and their genotypes, quality of product, and productivity levels. Thus, vegetable exploitation with adaptive and native crop plants is generally found to be most productive and stable under the dry-land climate of the state that receives 150–650 mm of rain and has problems from abiotic factors owing to climatic variability, extremes in temperatures, drought, frost, hot and cold winds, and soil and water quality.

Vegetable production in the country is largely uneven and concentrated, mainly in the limited states and areas where the climate is much more favorable and mild for their production. In addition, emphasis is given only to the few numbers of vegetables that are grown with high inputs and irrigations. To meet the increasing vegetable demand and utilization of indigenous resources, there is the utmost need for massive and integrated efforts with unconventional, under-scored, and perennial vegetable production. The increase in vegetable production can be achieved first by developing high-yielding crop genotypes suited to the prevailing conditions of the targeted zones and resource-based technologies under climatic variability. The second approach is to exploit native resources, i.e., varied and untapped land-areas and under-scored horticultural species with vegetable potentialities.

The inoculum dosage and method of application determines the efficacy of biofertilizers. The quantity of biofertilizers to be applied depends on the type of crop, seed rate and soil edaphic factors. There are 3 methods for inoculation of biofertilizers

Seed treatment: One packet containing inoculant (200 g) is mixed with 200 ml of rice gruel or 40 % gum arabic solution to make a slurry (Fig. 23). Then the seeds required for an acre are mixed in the slurry in such a manner that they have a uniform coating of the inoculant over the seeds. Then, the inoculated seeds are shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is normally sufficient to treat 10 kg of seeds.

1. INSECT- PESTS OF MANGO

1. Mango hoppers: Idioscopus niveosparus, I. clypealis, Amritodus atkinsoni (Cicadellidae: Hemiptera)

Management

• Avoid close planting, as the incidence becomes very severe in overcrowded orchards.
• Orchards must be kept clean by ploughing and removal of weeds.
• Pruning of dense canopy to facilitate aeration and sunlight.
• Avoid excess use of nitrogenous fertilizers.
• Immediately after withdrawal of monsoon (September-October), off-seasonal trunk spraying with Malathion 50 EC @ 15 ml in 10 lit of water suppresses the adult hoppers from multiplying further and hinders their oviposition and also kills the newly hatched nymphs.
• Avoid application of all other synthetic pyrethroids as they have been reported to cause resurgence of hoppers.
• If overlapping generations of hoppers are found, spray imidacloprid @ 3 ml or acetamaprid 4 gm in 10 litres of water at panicle emergence stage.

Important insect-pests of vegetable crops

1. Aphids: (Myzus persicae)

The aphids damage the crop by sucking the cell sap from the leaves as stems. The affected leaves in severe cases curl-up and rolled. The plant may be stunted, wither and die. There are three different species of aphids. They are greenish or black in colour.