eChapters

20.1. Introduction

Food laws and regulations are the legal standards of quality which regulate the manufacture and marketing of food products. The major objectives of enacting the food laws and regulations are:

1. To maintain quality of food produced

2. To prevent exploitation of the consumer by the sellers

3. To safeguard the health of the consumers and

4. To establish a standard of quality for different food products.

19.1. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. It is also defined as the combination of attributes or characteristics of the products that has significance in determining the degree of acceptability of the product to a user. Industry defines quality as the measure of purity, strength, flavour, colour, size, maturity, workmanship or any other distinctive attribute or characteristics of the product. According to USDA Marketing Workshop Report (1951) quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user . The quality standards of fresh and processed fruit or vegetable products vary with their intended use. For marketing purposes; size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind. While for processing, physico chemical attributes of raw material such as presence of soluble solids, development of uniform colour, flavour, juiciness, uniform maturity, tenderness in some vegetables etc are taken into consideration. During processing of fruit or vegetables into value added products; colour, flavour and texture etc also become important.

18.1. Introduction

Spoiled food is defined as the food that has been damaged or has undergone changes so as to render it undesirable/unacceptable/unfit for human consumption. Spoilage of processed products is mainly caused by the action of micro-organisms and due to the physical or chemical changes. The reasons for microbial spoilage include under- processing, inadequate cooling, contamination from leakage through can seams and pre-process spoilage. In under-processed cans (receiving low heat treatment) large number of micro-organisms is expected to be involved in spoilage.

18.2. Causes of food spoilage

Food spoilage or deterioration may be described as loss of weight, softening, souring, rotting, wilting, moulding or a combination of one or more forms. The major causes of food deterioration are: biotic and abiotic factors.

17.1. Introduction

Tea (Camellia sinensis L), coffee (Coffea arabica L.) and rubber (Hevea brasiliensis) are known as estate crops or conventional plantation crops. These plantation crops are perennial in nature and cultivation of these crops generates considerable employment opportunities during on farm and off farm processing activities. The plantation crops are high value industrial crops contributing about 25% of AGDP. India is a largest consumer of tea accounts for 22% global tea consumption and also accounts for one-fifth of global tea area. Indian coffee industry also have prime place among plantation crops and also contributing 4% coffee to global market. Similarly, rubber plantations also provides basic raw material to toys, balloon, tyres, seed oil cake and rubber wood factory. The plantation crops along with main processed products also produce large quantity of biomass like husk, coir dust, coffee husk, tea waste, shedding etc which can effectively used to reduce nutrient requirements of crops. Parchment coffee prepared by using the wet method is generally favoured by the market. Cherry coffee, due to its very nature of preparation and longer contact with the mucilage and fruit skin, is usually associated with a characteristic fermenting flavour.

16.1. Introduction

Plantation crops are high value industrial crops grown in the tropics play a vital role in agricultural economy and export trade of developing countries. Plantation crops have great potential for utilization of waste land like rainfed dry land, hilly, arid and coastal areas to provide nutritional security, earn foreign exchange and to ensure livelihood security. The major plantation crops grown in India are tea (Camellia sinensis L), coffee (Coffea arabica L.), rubber (Hevea brasiliensis), coconut (Cocos nucifera), arecanut (Areca catechu L.), oilpalm (Elaeis guinensis), cashewnut (Anacardium occidentale L.), cocoa (Theobroma cacao L.) and cardamom (Elettaria cardamomum). India is the leading producer of most of these plantation crops. Among all fruit crops, the processing plays an important role in plantation crops as they are generally consumed only after processing. In addition to primary products which are exported largely, some by-products from these crops also have industrial as well as commercial uses. Coconut, arecanut, cashewnut, black pepper and cardamom are known as small holder plantations whereas, crops like rubber, tea and coffee are known as estate crops or conventional plantation crop. Coir, wood and shell from coconut, leaf sheath in arecanut, cashew nut shell liquid (CNSL) and feni from cashew are some of by-products with a great impact and demand in the market.

15.1. Introduction

Freezing is one of the oldest and most widely used methods of food preservation, which allows preservation of taste, texture, and nutritional quality in foods better than any other method. It is a process of bringing down the temperature of food below its freezing point and the frozen storage generally refers at temperature below -18oC.

15.2. Theory of freezing

The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below freezing point and a proportion of water undergoes a change in state to form ice-crystals. Immobilisation of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause lowering of water activity of the food. Thus reduction in water activity and use of low temperature coupled with some pre- treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants and vegetables like green pea, green beans, sweet corn, spinach, sprouts and potatoes are examples of commercially frozen products.

14.1. Introduction

Tomato is grown in India in abundance both in summer and winters. Tomato though botanically a fruit is generally considered as vegetable because of the way in which it is consumed. Fresh tomatoes are very refreshing and appetizing but can not be stored for a long period. During peak season, about 25% of the produce is spoiled due to mishandling and such losses can be avoided by converting tomatoes into different value added products. Tomato can be processed into pulp, paste, puree, juice, ketchup and sauce. In India, tomato sauce and ketchup are very popular. High quality products can be prepared from tomatoes by:

1. Using uniformly ripened, red coloured tomatoes.

2. Avoid prolonged heating and cool the product immediately after cooking.

3. Avoid using iron and copper equipments and utensils at any stage of processing.

13.1. Introduction

The preservation by using common salt, spices and vinegar is a common method for preparation of pickles, sauce and chutneys. Salt is mostly used as preservative in pickles in combination with acid. Minimum concentration of salt to act as preservative is about 12%. It inhibits enzymatic browning/discoloration by acting as an anti-oxidant. It exerts its preservative action by:

• Causing high osmotic pressure and thus suppress the microbial cells.

• Dehydrating food by tying up the moisture thus making it unavailable for the growth of microorganisms.

• Salt in the food affects the solubility of oxygen and thus growth of aerobic microorganisms is inhibited

• Chlorine in sodium chloride being good oxidizing agent is toxic to microorganisms

• Salt increases the permeability of the cell wall and thus cause changes in the permeability. Only the haloduric microorganisms can tolerate these effects while others are unable to survive.

• Similarly addition of acid to the food lowers the pH of the food which inhibits the growth of spoilage causing microorganisms.

• Addition of spices and edible oil in these products besides improving flavour and taste also help in preservation.

12.1. Introduction

Drying refers to the method of removal of moisture content from the food to a level at which the activities of food spoilage and food poisoning micro-organism are inhibited. Drying under open sun is probably the oldest method of food preservation used for agricultural crops including food grains, oilseeds as well as fruits and vegetables. Most of the fruits and vegetables contain enough moisture to permit the activity of enzymes and micro-organisms for spoilage and drying is necessary to reduce the water activity. Therefore, reduction in water activity of the food is the main principle of preservation by drying. Two commonly used terms for dried foods are low moisture foods and intermediate moisture foods (IMF). During drying, a single layer of fruit and vegetables, either whole or sliced after primary pretreatments is spread on trays which are placed inside the dehydrator or in the open sun for drying. In mechanical dehydrator, the initial temperature is generally kept at 43oC which is then gradually increased to 60-66oC for vegetables and 66-71oC for fruits.

11.1. Introduction

A fruit preserve is a product made from properly matured fruit by cooking it whole or in the form of large pieces in heavy sugar syrup, till it becomes tender and transparent. A fruit impregnated with cane sugar and glucose, followed by subsequent draining and drying is called a candied fruit e.g. petha candy, ginger candy. Further, candied fruit covered or coated with a thin transparent coating of sugar, which imparts a glossy appearance is referred to as a glaced fruit. When candied fruit is coated with sugar crystals either by rolling it in powdered sugar or by allowing the sugar crystals from glucose syrup to deposit on it, it becomes a crystallized fruit. The use of high concentration of sugar in these products acts as the preservative. The products like Petha candy, aonla preserve, apple rings; candied citrus peels, ginger candy, ginger in syrup; bael, pineapple and carrot preserve are the commercial products in this category.

10.1. Introduction

Sugar acts as a preservative by osmosis and sugar is not a true poison fo micro organisms. It absorbs most of the available water and restricts the availability of water/moisture required for growth of micro-organism. The concentration of 68 70% sugar is used for preparation of jam and jellies. Sugar adds value to these products as it imparts sweetness as well as body to these products. When sugar is boiled with acid and fruits it is hydrolyzed into dextrose and fructose, the degree of inversion depending on the pH and duration of boiling. Because of inversion of sucrose, a mixture of sucrose, glucose and fructose are found in jams and jellies.

Jams, jellies and marmalades constitute an important class of preserved fruit products. Apple jam, pineapple jam, strawberry jam and mixed fruit jam prepared by using pulp of two or more fruits are quite common. Similarly, guava jelly and orange marmalade are also examples of such products.

9.1. Introduction

Fruit juice and beverages generally comprise of naturally extracted juices, drinks, ready to serve (RTS) beverages, nectars, squashes, cordials and appetizers etc. These products are highly refreshing, thirst quenching, appetizing and nutritionally superior to many synthetic and aerated drinks. Fruit juice is the natural liquid expressed by pressure or other mechanical means from the edible portion of the fruit. Fruit juices are generally extracted from fruits in a number of ways, depending on their structure and composition.

The composition of juice is unaltered during preparation and preservation while for fruit beverages like drinks, squashes, cordial etc the fruit juice or pulp, sugar, acid, colour, flavour etc are mixed in appropriate proportions to a desirable taste. Apple juice, orange juice, mango squash, guava drink, pineapple juice and squash, mixed fruit drink, mango nectar etc are the commercial products available in the market.

8.1. Introduction

The term canning refers to a process which involves heating food stuff in hermetically sealed containers for a specific time at specific temperature to eliminate microbial pathogens that endanger public health and micro-organisms as well as enzymes that deteriorate food during storage. The canning of fruits and vegetables is canned out in the season when they are available in plenty. The canned products are then sold in the off season for getting better returns and to assure availability of fruits and vegetables throughout the year at reasonable prices.

8.2. History of canning

Important historical developments in canning are as under:

• The credit for invention of canning goes to Nicholas Appert, a French confectioner who was awarded a prize in 1809 by the French government for developing new method of heat preservation of food in sealed container and after whose name the process of canning is known as Appertization.

• In year 1810, Appert published the first book on canning entitled The Art of Preserving Animal and vegetable substances for many years which is the first known work on canning.

• The work of Appert consists of packing food in glass bottles, closing with corks and heating the container in boiling water for several minutes depending upon type of food.

7.1. Introduction

Preservation of fruit and vegetables by application of heat is one of the important methods of food preservation as it not only imparts desirable effect in the eating quality of the food but also exerts a preservative effect on the foods by destruction of micro-organisms, enzymes, insects and parasites. Other advantages of heat processing include destruction of anti nutritional factors (e.g. trypsin inhibitor in some legumes), improvement in availability of some nutrients (eg improved digestibility of proteins, gelatinization of starches, and release of bound niacin), production of shelf-stable foods that do not require refrigeration and above all it offers a relatively simple control of processing conditions. The disadvantages of heat processing include alteration or destruction of sensory quality of foods like flavor, colour, taste and texture as a result the heat processed foods are perceived to have different quality as compared to fresh produce.

6.1. Introduction

Chemical preservatives are those substances which minute organoleptic and physico-chemical properties . Preservation of food products with chemical preservatives is usually based on the combined or synergistic activity of several additives, intrinsic product parameters (e.g. composition, acidity, water activity) and extrinsic factors (e.g. processing and storage temperature).

Many chemical substances are added to the food for functional purposes and are called as additives and in many cases these are found to occur naturally in some foods. An additive is defined as a substance or mixture of substances other than basic food ingredient present in a food as a result of any aspect of production, processing or packaging.

5.1. Introduction

Food processing units range from home-based businesses to large-scale processing plants. The food processing sector is a highly fragmented industry which includes fruit, vegetables, dried, preserved, canned, pulps, pickles, chutneys, milk, dairy products, ready-to-serve products, frozen desserts, meat, poultry, marine products, grain processing, beer and alcoholic beverages, consumer, convenience and packaged food, soft drinks etc.

The main objective of fruit and vegetable processing is to supply wholesome, safe, nutritious and acceptable food to consumer throughout the year. Fruit and vegetable processing also aim to replace import of products such as squashes, jams, tomato sauces, pickles, etc., besides earning foreign exchange by exporting finished or semi-processed products. The fruit and vegetable processing industries are also established in the developing countries due to following reasons:

• Reduction in fruits and vegetables post harvest losses

• Industrialization policy of the government

• Generate both rural and urban employment

• Improve farmer s nutrition by self consumption of processed fruits and vegetables during off season

4.1. Introduction

Food occupies first position among the basic needs of human beings. Food processing dates back to the prehistoric age when crude processing including various types of cooking, such as over fire, smoking, steaming, fermenting, sun drying and preserving with salt were in practice. These crude processing techniques remained essentially the same until the advent of the industrial revolution. However, the change in food habits and the quality consciousness of the consumers in the developed world raises the need of advancements in development of food processing like spray drying, juice concentrates, freeze drying and also introduces dried instant soups, reconstituted fruit juices, and self cooking meals such as ready-to-eat food rations etc., during 20th century.

3.1. Introduction

Food preservation can be defined as the science which deals with the process of prevention of decay or spoilage of food thus allowing it to be stored in a safe condition for future use. Scientifically, preservation may be defined as a science which deals with the process for prevention of decay. All foods gradually undergo deterioration or spoilage from the time they are harvested or manufactured. Some commodities spoil rapidly, while others can keep for longer but limited periods. Spoilage in the food products may be caused due to some physical, chemical and microbial changes during storage. The preservation of these products can be resulted by controlling following physical, chemical and microbial changes.

2.1 Introduction

Food Pipeline refers to a post harvest system which deals with ensuring the delivery of a crop from the time and place of harvest to the time and place of consumption, with minimum loss, maximum efficiency and returns to all concerned including grower, processors and consumer. The term system represents a dynamic, complex aggregate of locally interconnected functions or operations within a particular sphere of activity. While, the term pipeline refers to the functional succession of various operations but tends to ignore their complex interactions.

2.2 Stages of postharvest system

The food commodities broadly undergo different stages/operations of post harvest systems right from harvest till consumption.

1. Harvesting

2. Threshing (food grains, pulses and oilseeds)

3. Drying (food grains, pulses and oilseeds)

Fruits and vegetables are important supplement to the nutritional requirements in the human diet as they provide the essential minerals, vitamins, flavonoids/ antioxidants and dietary fiber required for maintaining health. India is the second largest producer of fruits and vegetables in the world after China. During 2012-13, fruits and vegetables have registered a production of about 77.7 million MT and 159.5 million MT respectively (NHB, 2013. However, increase in production has not matched with the improvement in infrastructure for post-harvest management and processing. Fruits and vegetables are highly susceptible to deterioration owing to their high moisture content and delicate nature. Besides, most of the fruits and vegetables are seasonal in nature leading to glut in the local market during peak season resulting in huge economic losses to the growers. According to an estimate, 6-18% of fruits and vegetables are lost in the supply chain right from harvest to consumption due to lack of infrastructure for postharvest management, processing and value addition (Nanda et al., 2012).

Previous Year Questions

1. Intellectual property rights (IPR) are the rights given to persons over the creations of their minds: inventions, literary and artistic works, and symbols, names and images used in commerce. They usually give the creator an exclusive right over the use of his/her creation for a certain period of time?

2. Plant varietal protection?

• The importance of intellectual property was first recognized in the Paris Convention for the Protection of Industrial Property (1883) and the Berne Convention for the Protection of Literary and Artistic Works (1886). Both treaties are administered by the World Intellectual Property Organization (WIPO).

Previous year questions

1. Introduction?

2. Role of private? Introduction

• The Indian seed industry used to be dominated by public sector seed companies. However, following the easing of government regulations and the implementation of a new seed policy in 1988, the private sector seed companies have started playing a major role in seed development and marketing.

• More recently, the government’s decision to embrace biotechnology as a means of achieving food security has attracted several leading biotechnology-focused multinational seed companies to India.

• The composition of the seed industry, by volume of turnover, has reportedly reached a ratio of 60:40 between the private and public sectors.

Previous Year Questions

1. What is DNA fingerprinting? Discuss the applied aspects in modern agriculture. (15M, CSE 2021)

• The term DNA fingerprinting in its original sense refers to the method developed in 1985 by Sir Alec Jeffreys (Jeffreys et al., 1985) and his associates for the simultaneous detection of highly variable DNA fragments by hybridisation of specific multilocus probes to electrophoretically separated restriction fragments?

• The DNA fingerprints, resembling barcodes, are unique to the individual and hence can be used in much the same way as conventional fingerprints - to identify individuals with absolute certainty?

DNA Fingerprinting Techniques

• A large number of fingerprinting techniques are in currency, which are variations and combinations of restriction fragmentation and polymerase chain reaction (PCR).

• Restriction fragment length polymorphism (RFLP) involves digesting DNA with restriction enzymes, separating the resultant DNA fragments by gel electrophoresis, blotting the fragments to a filter and hybridising probes to the separated fragments. Specific probe-enzyme combinations give highly reproducible patterns.

• With the advent of PCR technology a number of simpler techniques have become available for molecular characterisation.

• These include, random amplified polymorphic DNA (RAPD) and its several variants, intersimple sequence repeats (ISSR), amplified fragment length polymorphism (AFLP) and sequence tagged microsatellites sites (STMS).

Previous Year Questions

1. Different Classes of Seeds?

2. Various parameters of field & seed standards for breeder seed production?

3. Factors that affect the longevity of seeds during storage in warehouses?

4. Initial Seed Quality?

5. Seed Testing?

Different Classes of Seeds

1. Nucleus Seed: It is handful of seed maintained by concerned breeder for further multiplication. The nucleus seed will have all the characters that the breeder has placed in it and it is of highest genetic purity. The quantity of nucleus seed is in kilograms.

2. Breeder Seed (BS): Breeder seed is a seed or vegetative propagating material directly controlled by the originating or sponsoring plant breeder of the breeding programme or institution and or seed whose production is personally supervised by a qualified plant breeder and which provides the source for the initial and recurring increase of foundation seed. Breeder seed is exempted from certification. However, the breeder seed crop is being inspected at appropriate stages by a Monitoring Team consisting of breeder, representative of NSC and representative of Seed Certification Agency. The breeder seed lots are affixed with Golden Yellow tags. The genetic purity of breeder seed crop should be maintained at 100 per cent.

Previous Year Questions

1. What is seed treatment?

2. Factors affecting the seed quality of a crop during seed production?

3. Seed treatment campaign – Role & importance?

4. Agronomic and genetic principles of quality seed production?

5. Varietal seed production and hybrid seed production?

Seed Treatment

Seed treatment refers to the application of fungicide, insecticide or both to the seeds to disinfect(deep seated) and disinfest (over seed coat) them from seed borne or soil borne pathogenic organisms and storage insects. It also refers to subjecting the seed to solar energy exposure or immersion in conditional water.

Principle of ICP Optical Emission Spectrometry

The ICP is a partially ionized gas (mostly Ar) plasma produced in a quartz torch using a 1–2.5 kW radio frequency power supply. Samples are typically introduced into the center of the plasma as aerosols. Compared to the atomic absorption spectrophotometers, in which the excitation temperature of the air acetylene flame measures 2000–3000 “K, the excitation temperature of the argon ICP is 7000–10,000 K, which efficiently excites many elements. The solution for analysis is carried by a peristaltic pump through a nebulizer into a spray chamber, where aerosol is produced by the argon gas. The aerosol thus generated is directed into the central channel of the plasma. The plasma in ICP is generated at the end of a quartz torch by a water-cooled induction coil through which a high-frequency alternate current flows. Consequently, an alternate magnetic field is induced, which accelerates electrons into a circular trajectory. Due to the collision between the argon atoms and the electrons, ionization occurs, giving rise to stable plasma. At the core of argon plasma, the temperature is sustained at approximately 10,000 K, where aerosols are quickly vaporized. Subsequently, atomization and ionization of the sample analyte elements take place. Due to the thermic energy taken up by the electrons, they reach a higher “excited” state. When the electrons drop back to ground level, energy is liberated as light (photons). Each element has its own characteristic emission spectrum. By means of an Echelle grating, a prism, and a focusing mirror, these emitted photons at various frequencies are captured simultaneously by the suitable detectors. The wavelength of the photons can be used to identify the elements from which they originate.

India is the second-largest producer of fruits in the world after China. However, the average productivity of fruits in India could be higher compared to many developed countries. The main reasons for low productivity are old and senile orchards, low-yielding varieties, poor orchard management, and inadequate technological upgradation and adoption by the growers. Presently, due to urbanization and population explosion, there is a continuous decline in the availability of cultivable land; rising energy and land costs, together with the increased demand for horticultural produce, have given thrust to the concept of high-density planting (HDP) in fruit crops. However, at the same time, it is now realized that there is limited scope for a quantum jump in fruit production in traditional areas. Therefore, the alternative is to expand under non-traditional regions like arid and semi-arid areas of the country. India’s hot arid and semi-arid regions are spread mainly in Rajasthan, Gujarat, Andhra Pradesh, Telangana, Punjab, and Haryana. The vast land resources offer an excellent opportunity for fruit production in the Indian arid zone, which is spread over about 31.70 m ha, of which 41.50% is arable and 19% is a cultivable wasteland (Table 1).

Introduction

The term horticulture first appeared in English literature in 1678 in the treatise “The New World of English” authored by E. Phillips in London. In ancient scriptures, the garden is mentioned in Vatsayan. During the ancient period, gardens were the sole means of recreation, leisure, and pleasure. The planting of trees was undertaken for the purpose of shade, shelter, and fruits. It is quoted the queen of Raja Dashrath made a question that “What is the meaning of planting trees and garden”. Raja Dasharath responds that “the very meaning of planting trees and taking fruit plantation is the shade and fruits to the passersby”

Horticulture accounts for about 30% of India’s GDP from 13.08% of the cropped area. It stands for about 37.1% of the total export of agricultural commodities. The country shares about 13.6% of the fruit production and 14% of vegetable production (Indian Hortic Database, 2014). Horticulture shares for more than 33% of agricultural production. The horticultural production touched the mark of 300.64 million tonne during 2016–2017 which marks the sixth straight year horticultural production outstripping that of food grain production estimated at 276 million tonne in 2016–2017. It stands witness to structural changes in Indian agriculture in which the farmers have increasingly adopted the cultivation of commercial horticultural crops due to increasing market demand and quicker cash flow.

Introduction

Modern-day agriculture and civilization together demand increased production of food to feed the global population. New technologies and solutions are being applied in the agricultural domain to provide an optimal alternative to gathering and processing information while enhancing net productivity. At the same time, the alarming climate change and increasing water crisis demand new and improved methodologies for modern-age agricultural and farming f ields. Automation and intelligent decision-making are also becoming more important to accomplish this mission in flower production. In this regard, the Internet of Things (IoT) ubiquitous computing, wireless ad-hoc and sensor networks, radio frequency identifier, cloud computing, remote sensing, etc. technologies are becoming increasingly popular.

Most of the IoT-based applications are targeted for various applications. For example, IoT for environmental condition monitoring with information on soil nutrients are applied to predict crop health and production quality over time. Irrigation scheduling is predicted with IoTs by monitoring the soil moisture and weather conditions. The world’s first IoT device was invented in the early 1980s at Carnegie Melon University.

The National Bureau of Plant Genetic Resources (ICAR-NBPGR) was established in 1976 in New Delhi by the Indian Council of Agricultural Research (ICAR). The mandate of the Bureau was to act as a nodal institute for the acquisition and management of indigenous and exotic plant genetic resources (PGR) for agriculture and to carry out related research and human resources development for sustainable growth of agriculture. As we know our country is a mega-diverse country with 2.4% of the land area, accounting for 7.8% of the recorded species of the world spread over 45,968 (11.18% of world) species of plants and 91,212 species of animals (7.43% of the world) that were documented in its 10 bio-geographic regions. India shares four biodiversity hotspots, namely Western Ghats and Sri Lanka, the Himalayas, Indo-Burma, and Sundaland, and one third of our species of higher plants are endemic. Out of 45,968 plant species, 5150 are endemic and distributed in 141 genera under 47 families, corresponding to about 30% of the world’s recorded flora, which means 30% of the world’s recorded flora is endemic to India. The plant genetic resources are fundamental to human welfare and their effective management is a crucial for conservation of genetic variability. As of today, ICAR-National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi, India has conserved about 4.6 lakh accessions of 1900 species in its gene bank, and about 6900 accessions of intermediate species have been conserved in cryobank. The Bureau not only conserve PGR safely to meet the needs of future generations but also provides these to the nation’s crop improvement programs to sustain continued advances in agricultural productivity and stabilize production. ICAR-NBPGR is the nodal agency for import and export of all PGR for research purpose, adhering to guidelines of National Biodiversity Act, 2002.

Water is a critical input for agricultural production and plays an important role in food security. Irrigated agriculture represents 20% of the total cultivated land and contributes 40% of the total food produced worldwide. Irrigated agriculture is, on average, at least twice as productive per unit of land as rainfed agriculture, thereby allowing for more production intensification and crop diversification. India has 18% of world’s population, having 4% of the world’s fresh water, out of which 80% is used in agriculture. India receives an average of 4000 billion cubic meters of precipitation every year. However, only 48% of it is used in India’s surface and groundwater bodies. In India, more than 60% population is still dependent directly or indirectly on agriculture. Most of the available water (more than 90%) is used in agriculture. From 1951 to 1997, gross irrigated areas expanded fourfold from 23 million ha to over 90 million ha, and irrigation continues to be the largest water user. The water availability in India is going to be worse as the agriculture-important states like Punjab, Haryana, Tamil Nadu, and Rajasthan are facing a steady fall in underground water table, and per capita availability of water fall from 3450 cubic meter (1951) to 1250 cubic meter (1999).

Introduction

Salinity is a significant abiotic stress faced by plants, particularly in semi-arid and arid regions of the world, where annual evaporation exceeds precipitation. In addition, approximately half of all the world’s irrigated areas are subjected to secondary salinization, alkalization, and waterlogging. About 8.85 million ha of land in India is degraded due to salinity and alkalinity problems. Besides the naturally occurring salt-affected soils, the extent of man-made salinized soil is also significant. Salt accumulation in the root zone layers of soils becomes a major constraint to agricultural productivity. However, such lands can effectively be utilized for salt-tolerant biological systems.

To survive under high salt concentrations, some plant species evolved well adapted morphological and physiological characteristics, allowing them to proliferate in saline environments. These species are known as halophytes. Halophytes constitute a small fraction of total plant species; they represent a wide diversity of plant forms and habitats and are of immense significance in expanding salinity problems and decreasing freshwater supply for agriculture. These salt-tolerant species possess enormous taxonomical plant forms and habitat diversity. Taxonomically, the species belonging to Poaceae, Cyperaceae, Chenopodiaceae, and Asteraceae families constitute a significant share of salt tolerant species of north-western hot arid regions of India. In the herbaceous plant group, the species of the family Poaceae are most abundant. In the case of woody perennials, Chenopodiaceae, Tamaricaceae, and Salvadoraceae families are essential as true halophyte and facultative halophyte species.

Introduction

Despite environmental, bio-physical, and resource constraints in most parts of Rajasthan, even though the state’s geography can be classified into three distinct climatic zones: hot arid, semi-arid, and sub-humid, from a horticultural perspective. These diverse zones have excellent vegetable production potential, a wide range of crop plants, and varied opportunities for resource utilization. However, extremes of high (March–October) and low (December–January) temperature situations associated with abiotic stresses in arid regions restrict the choice of crops and their genotypes, quality of product, and productivity levels. Thus, vegetable exploitation with adaptive and native crop plants is generally found to be most productive and stable under the dry-land climate of the state that receives 150–650 mm of rain and has problems from abiotic factors owing to climatic variability, extremes in temperatures, drought, frost, hot and cold winds, and soil and water quality.

Vegetable production in the country is largely uneven and concentrated, mainly in the limited states and areas where the climate is much more favorable and mild for their production. In addition, emphasis is given only to the few numbers of vegetables that are grown with high inputs and irrigations. To meet the increasing vegetable demand and utilization of indigenous resources, there is the utmost need for massive and integrated efforts with unconventional, under-scored, and perennial vegetable production. The increase in vegetable production can be achieved first by developing high-yielding crop genotypes suited to the prevailing conditions of the targeted zones and resource-based technologies under climatic variability. The second approach is to exploit native resources, i.e., varied and untapped land-areas and under-scored horticultural species with vegetable potentialities.

The hot arid regions of India are spread over an area of about 31.7 million ha, mainly in the states of Rajasthan, Gujarat, Andhra Pradesh, Punjab, and Haryana, which inhabit an average of 61 people per square km, making up a population of nearly 20 million people. The Indian arid zone is characterized by high temperature and low and variable precipitation, which limit the scope for high horticultural productivity. However, these conditions greatly favor the development of high-quality production in a number of fruits, such as date palm, ber, pomegranate, citrus, aonla, bael, grapes, and guava. The optimized technologies and inputs could increase the existing low productivity. It is now realized that there is a limited scope for a quantum jump in fruit and vegetable production in the traditional production areas. The amelioration of the extreme conditions is also considered vital for the life support of the inhabitants of this area. The recent awareness regarding the potential of these ecologically fragile lands for the production of quality horticultural produce has not only opened up the scope for providing economic sustenance for the people of this region but also for bringing new areas to increase production through horticulture. The scope of expanding water supplies through the development of water resources is very limited since suitable dam sites are fewer, development costs are prohibited, and environmental concerns are too strong. This has led to the realization that saving on the use of water is an option for expanding the water supply.

Introduction

Stress is any unfavorable condition for a plant’s normal growth and development. In the case of crop plants, such adverse conditions are caused by biological (biotic) and non-biological (abiotic) environmental factors. Abiotic stress is the negative impact of non-living factors on living plants in a specific environment. Usually, abiotic stress is unavoidable, and we can only go for its management.

The most basic stresses include:

• Extreme (high/low) temperatures

• Soil-chemical properties

• Water deficiency

• Excessive moisture

• Element toxicity or deficiency

Salt-affected soils (SASs) are an important ecological entity in world agriculture. It varies with time and space depending on soil, rainfall, irrigation, microtopography, groundwater quality, and depth. Weathering of rocks and minerals, higher rates of evapotranspiration, deposition of wind-blown salt sediments, and seawater ingress are the significant factors contributing to the formation of SASs. Large acreage in canal-irrigated tracts of arid and semi arid areas, which suffers from water logging, is also responsible for secondary salinization. According to an estimate, about 33% of irrigated lands worldwide are affected by varying salinity and sodicity. Other reasons other than improper irrigation that cause soil salinization include deforestation, which results in salt resettling at upper and lower levels, chemical contamination, and air-borne and water-borne salts from industrial outpouring. In addition to soil salinity, the problem of poor-quality water would also significantly increase in the foreseeable future due to planned expansion in irrigated areas and intensive use of natural resources to fulfill the increasing population’s food and other livelihood requirements. Groundwater salinity under irrigated agricultural lands may or may not increase over time. The groundwater salinity will remain the same if the natural river water is used for irrigation. However, if the irrigation water is polluted and contains salts, the salinity of groundwater may show an increasing trend. The rate at which groundwater salinity may increase would depend upon the quality and quantity of recharge water, the depth of the hardpan/barrier layer under the groundwater table, and the substrate’s porosity.

Introduction

The Indian arid zone, which occupies 38.7 million hectares in the states of Rajasthan, Gujarat, Punjab, Karnataka, and Andhra Pradesh, is characterized by low and erratic rainfall with a coefficient of variation fluctuating from 40 to 70% and extremes of temperature (1–48º C), poor-quality underground water, high wind velocity, and sandy soils. These vast land resources are blessed with rich agro-biodiversity despite all these aberrations that support the high human and livestock populations and have beneficial climatic conditions for producing quality fruits and vegetables. Recently, the immense potential of arid horticulture has been realized, and the area under cultivation of arid crops is increasing enormously. The production also increases with the increased cultivated area, which opens up the probability of establishing a market glut with underutilized foods.

Arid Fruits and Vegetables

Arid and semi-arid regions of the country are blessed with a variety of horticultural crops such as date palm, bael, aonla, pomegranate, fig, mulberry, Jamun, ker, karonda, lasora, wood apple, ber, bordi, khejri, kachri, snap melon, drumstick, cluster bean, and methi. Most of these crops are highly perishable and cannot be stored for extended periods due to uncongenial atmospheric conditions such as high temperatures and low relative humidity.

Seed is the basis of agriculture. It is the cheapest and most critical input for sustainable crop production. The response of all other inputs depends upon the quality of the seed to a large extent. The direct contribution of quality seed alone to crop production is about 15 to 20%, and it can be further raised to 45% with improved crop management practices. Quality seed ensures good germination and vigorous growth, resulting in a good crop stand. The Green Revolution witnessed in the country during the mid-sixties resulted from the seed revolution in wheat and rice. After that, there was a consistent increase and record production in food grains, oilseeds, pulses, and all other crops, including fruits and vegetables, due to the development of high-yielding varieties.

The seed itself is a technology that is inherited in a capsulated form. Therefore, the seed sector plays an essential role in disseminating the most recent technology to farmers through the quality seed of high-yielding varieties. The success of any developmental program in agriculture, like NFSM, RKVY, oilseed mission, horticulture mission, etc., depends upon the availability of good quality seed and planting material. Seed security is a prerequisite for food and nutritional security. Therefore, recognizing the facts, many emphases are being given to seed replacement and varietal replacement rates. The growth of the Indian seed industry is faster than the global rate.

Introduction

Ber belongs to the family Rhamnaceae, which consists of about 45 genera and 950 species. Out of 950, the genus Ziziphus consists of about 80 species of evergreen and deciduous climbers, shrubs, and trees found in the old world tropics, subtropics, and warmer temperate regions. The fruits of almost all species are edible, and jujube (Z. jujuba Mill.) and ber (Z. mauritiana Lamk.) are cultivated commercially. Liu and Cheng (1995) believed that the Indo Malaysia region (South and South East Asia) is the center of both the evolution and distribution of the genus Ziziphus.

Ber is the most common fruit of arid and semi-arid parts of the world due to its hardy nature and wild adaptability to soil and climatic conditions. It survives and produces economically viable yield in the hyper-arid zone where most of the trees fail to grow due to lack of moisture, extreme temperature, and poor soil conditions. It performs well under saline and alkaline soil conditions. It can produce economically viable yield with moderately saline irrigation water. Ber can withstand extreme heat but is susceptible to frost. Furthermore, due to its deep root system and other xerophytic characteristics, it is extremely drought tolerant but requires adequate soil moisture during fruit development. While high atmospheric humidity is unsuitable for ber cultivation, low humidity during fruit development improves fruit quality.

Introduction

Pomegranate (Punica granatum L.) is an important fruit crop in arid and semi arid regions. In India, it is grown commercially in Maharashtra, Gujarat, Karnataka, Andhra Pradesh, Telangana, Tamil Nadu, Madhya Pradesh, and Rajasthan. India is one of the largest producers of pomegranate in the world. During 2020–2021, pomegranate was cultivated over 2.71 lakh ha with an annual production of 30.88 lakh tonnes and a productivity of 11.39 tonnes/ ha in India. Maharashtra is the leading state, with 63% of the area and 62% of total production. In India, pomegranate is available throughout the year (January–December). India has the largest area of pomegranate cultivation globally.

Pomegranate can tolerate heat, drought, and moisture deficit. The area under pomegranate cultivation in India is increasing daily due to its high demand, hardy nature, low cost of cultivation, high yields, better storage quality, and therapeutic values. It has enormous medicinal and nutritional value and is one of the richest sources of antioxidants. Several processed products such as juices, squash, jelly, anardana, and mouth fresheners are prepared by fruit processing. The fruit is mainly used as an ingredient in cooling and refrigerant mixtures and in the preparation of juice, concentrates, condiments, and pastes. The juice is highly nutritious and is recommended for patients suffering from gastric troubles. It contains 16.5% TSS and 0.33% acidity; total sugars, 13.93; reducing sugars, 13.60 and non-reducing sugars, 0.33%; and 9.27 mg/100 g ascorbic acid.

India is the second-largest producer of fruits in the world after China. As per the National Horticulture Database (3rd Advance Estimate), the area under cultivation of fruits is 6.914 million hectares with a production of 103.027 million tonnes. Mango (34.71%) ranks 1st in the area, followed by citrus (15.42%), banana, and grapes (2.14%), standing in the 9th position. Likewise, Mango (22.4%) occupies the 2nd position in production, followed by Citrus (12.89%) and Grapes, sharing around 3%. Underutilized fruit crops, such as aonal, bael, ber, Jamun, and tamarind, and minor fruit crops like phalsa, karonda ker, salvadora, etc., are grown in drier tracts of the country, including the arid region. Some of the crops introduced as non-traditional crops have revolutionized the fruit industry in India. Although the total acreage of cultivation and production of fruit crops has accelerated annually, the productivity of fruit crops has been on a declining trend due to several factors impacted by abiotic stresses. Since plants’ first line of defense lies in the roots, the success or failure of orcharding enterprises depends upon rootstocks.

Introduction

Date palm (Phoenix dactylifera L.) is a monocotyledonous and dieocious species belonging to the family Palmae. The Latin name of the date palm, Phoenix dactylifera, is derived from the Phoenician name “Phoenix,” which means date palm, and dactylifera is derived from the Greek word “daktulos,” which means a finger. In India, generally, two species of Phoenix are found, of which, Phoenix sylvestris Roxb. called “desi khajoor,” is found throughout the country but t produces inferior quality fruits. The fruit contains large stones with less pulp content.

Date palm is considered one of the world’s oldest cultivated fruit trees. Archeological evidence suggests that the date palm was domesticated some 6000 years ago in the Mesopotamian region. It is widely cultivated in arid regions of the Middle East and North Africa. It is also one of the most appropriate fruit trees, and it can be easily grown under saline soil and water conditions. It grows well in poor, arid soils due to its hardy plant characteristics and deep root system. Date palm is believed to be indigenous to countries around the Persian Gulf. In the Indus Valley, date palm is believed to have been introduced by the soldiers of Alexander the Great in the fourth century B.C. and during the Muslim invasions at the beginning of the eight century AD. Date palm was commercially cultivated in Iraq, Saudi Arabia, Algeria, Iran, Egypt, Morocco, Tunisia, Pakistan, and Sudan, among many other Near East and North African countries. In India, dates are grown in the states of Gujarat, Rajasthan, Punjab, Haryana, and Tamil Nadu (Table 1).

Background

Water is the most precious input for dryland horticulture, and therefore, its use should be planned judiciously. Rainwater harvesting and its management play a significant role in horticultural production. The rainwater can be diverted either directly to tree basins in situ or in suitable structures ex situ, which can later be utilized as lifesaving irrigation. In sandy soils, in situ conservation should be popularized, while in heavier soils, ex-situ conservation should be popularized. The water thus collected remains stored deep into the soil profile, escapes from evaporative losses, and is available during critical periods of demand. Perennial fruit trees, once established, get the advantage of any time it rains during the year, unlike annual crops. Water harvesting in khadins in parts of the Jaisalmer district of Rajasthan and its use in raising crops and trees is the age-old practice of in situ water harvesting. The hilly, rocky, and sloppy lands generate runoff, which can be collected in ponds or tanks and recycled for the establishment of fruit plantations and lifesaving irrigation at critical stages. In the same stance, nutrient management also plays an important role in the successful cultivation of horticultural crops under dryland. The arid zone soils are very poor in the majority of nutrients except potash. Enrichment of soil with organic and inorganic sources is essential for optimum production of fruits and vegetables. The application of chemical fertilizers has made miracles initially in agriculture and horticulture. However, increasing awareness of environmental degradation due to various anthropogenic activities has been expressed worldwide about the over-reliance on mineral fertilizers, which causes environmental penalties. It has also led to a deficiency of several micronutrients. Therefore, the option left is whether to revert back to traditional farming using organic input or to follow some middle path by integrating both organic and inorganic. So far as present knowledge is concerned, organic manuring alone cannot maintain the required productivity and demand for food.

The Indian arid zone is one of the largest subtropical deserts in the world, of which 20% is arid and the rest is semi-arid. A hot arid zone is spread over 31.7 million ha and confined to Rajasthan, Gujarat, Andhra Pradesh, Punjab, and Haryana. The major part of the hot arid region of the country lies in western Rajasthan (19.62 million ha), followed by north-western Gujarat (2.16 million ha). It is characterized by a high aridity index, extremes of temperature, low and variable precipitation, very high wind velocity and dust storms, high soil pH, a high infiltration rate, and minimal availability of groundwater and saline ground irrigation water. Under such harsh climatic conditions, cultivating vegetable crops in open conditions results in meager and poor-quality yield, which fetches less return per unit area. By creating a suitable microclimate for plant growth throughout the year, vegetables can be cultivated under adverse climatic conditions. It is possible through protected cultivation, which has tremendous scope in peri-urban areas of the country. It is the best way to increase the productivity and quality of vegetables, especially cucurbits. India is the world’s second-largest producer of vegetables, next only to China.

Introduction

The ill effects of chemicals used in agriculture have changed the mindset of consumers in different countries, who are now buying organic products with a high premium for health. Policymakers are also promoting organic agriculture for the restoration of soil health and the generation of the rural economy, apart from making efforts to create a better environment. The world principles of organic farming have emerged in the form of biological farming, ecological farming, permaculture, biodynamic agriculture, green farming, bio-intensive farming, zero-input agriculture, etc..

Organic agriculture is one of the broad spectrums of production methods that are supportive of the environment. Organic production systems are based on specific standards precisely formulated for food production and aim at achieving agroecosystems that are socially and ecologically sustainable. Organic farming practices help in increasing carbon sequestration, soil and water conservation, and minimizing risk in agriculture. The important aspects of organic farming with respect to water conservation are mentioned in this chapter.

Nutraceuticals have received considerable attention for their expected safety and potential nutritive and therapeutic effects. These were used as alternatives to modern medicines that promote quality of health, increase the nutritional value of the diet, and prolong life expectancy. Major constituents of nutraceuticals are herbals; various nutrients and dietary supplements are involved in preventing different diseases and minimizing the pathophysiology of the disease too. It also acts as an immune boosting, natural antioxidant, anticancer, anti-inflammatory, anti-diabetic, cardioprotective, and organ protective agent, in addition to different health-promoting effects. Ultimately, they ensure a better quality of life.

The scope of the nutraceutical field is plenty both in terms of type and the varieties. The nutraceuticals industry in India is one of the rapidly growing markets. Higher- and upper-middle-class consumers are perceiving nutra ceuticals as alternatives to prescribed drugs and exclusively for their beneficial properties without any side effects. Consumers are showing sharp interest in nutraceuticals for boosting energy and improving their physical endurance and mental alertness. Nutraceutical industries are focusing on developing new products with innovative formulations and using proper advertisements to choose the right products. Nutraceuticals have significant promise in the promotion of human health and the prevention of disease. They are widely accepted by all age groups due to their safety, higher quality, purity, efficacy, health-promoting, and disease-curing activities. The newest trend is moving toward nutraceuticals, leading to a new era of medicine and health. It is still in its infancy in India. But in this hype era, we must say, “let food be your medicine” and “proper nutraceuticals daily can keep the medicine away.”

Water is a very essential component for human civilization, living organisms, and natural habitats. It ma.kes up a major part of plant biomass (about 90% of plant cytoplasm; Singh, 2014). Irrigation, the largest water user sector, is currently under pressure to produce more with higher quality by using limited water to feed the increasing population of the country. The highest proportion of water is consumed by agricultural crops, which is called the blue water footprint, and it is assumed that the water scarcity will increase in the future in view of the changing climate. India has 18% of the world population; however, the water resources are only 4%. Even with all the planning and development, the country has not even been able to provide sufficient drinking water to all at the end of the 20th century. The water sector is facing a number of challenges regarding the availability, accessibility, use, and sustainability of its fresh water resources. Further, the distribution of water in the country is also uneven over space and time. Unfortunately, Rajasthan is the driest state in the country and has been water scarce (with per capita water availability below 1000 m3/year) since 1991. The efficient use of water in agriculture/ horticulture without affecting crop productivity or quality by adopting water saving irrigation techniques is the foremost need. Fruit crops, which have gained commercial attention in recent years, are an important component of Indian agriculture. Due to low irrigation and water use efficiencies, irrigation managers are looking for the introduction of effective water-saving devices and practices for water management in fruit crops.

In irrigated agriculture, water is the most important and precious input among all the inputs required for the biological activities of the plant. It is also a key input not only for ensuring food security but also for sustainable socio economic development. Water plays a crucial role in enhancing agricultural production and provides food security. On a global scale, 97.5% of water resources are salty ocean water, with only only 2.5% being fresh water. The bulk of the latter occurs in the polar ice and glaciers, as well as deep aquifers, some in water bodies like rivers and lakes, and in soil moisture. On the whole, less than 1% of the world’s fresh water resources are available for human use. The world’s average annual precipitation of about 1100 mm, marked by wide variation in water availability, is creating a great divide in regions, resulting in plenty of regimes and scare/acute shortage regions.

In India, the average annual precipitation, including snowfall, is 4000 billion cubic meter (bcm), of which water availability in the river systems is estimated at 1869 bcm, and out of which only 1122 bcm can be used. About 690 bcm is surface water and 432 bcm is ground water. As compared to the world’s average annual rainfall of 1100 mm, India receives 1170 mm, which is highly erratic and uncertain in both time and space. Thus, in India, very limited fresh water is available for use because of competitive and conflicting demand from the agriculture, industry, power, and domestic sectors, which is expected to increase from 10.7% in 1990 to 22.5% in 2025. While that of agriculture will decrease from 89.3% in 1990 to 77.6% in 2025. The projected requirement of water for use by the different sectors shows an increase in energy and industry as compared to the domestic and irrigation sectors by 2025, indicating a growing scarcity of water availability in the future.

A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.