ICAR ARS 2024-2025 (PRE.ONLY) (ICAR ARS 28 VETERINARY MICROBIOLOGY 2024)

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Eligibility

Master’s degree in Veterinary Sciences with specialization in Microbiology/ Bacteriology/ Virology/ Mycology/ Immunology. 

Exam Pattern

"Two separate competitive written  examinations (Computer Based Test + Written) followed by Viva-voce shall be conducted as per the following plan of examinations:-
Examination               Max. Marks  Duration
Preliminary-ARS         150              2 hours (Objective Type)
ARS – Main                   240             3 hours  (Descriptive Type)
Viva-voce                        60               1/3 marks will be deducted for each wrong answer in ARS-2021 (Preliminary)
Examination (Objective Type). There will be no rounding off of fractions of marks. This is a qualifying examination and marks scored will not be counted for final selection.                                              ARS-2021 (Mains) Examination Paper will have only one paper of 240 marks in the respective disciplines, to be attempted in 3 hours duration. The paper shall be divided in three parts A, B and C. Part ‘A’ will consist of 40 (forty) questions of 2 (two) marks each. In this 30 part, answers required will be of very short, not exceeding 10 (Ten) words at the most. Part ‘B’ will have 20 (twenty) questions of 5 (five) marks each requiring one or two paragraphs and/or graphic explanation. Part ‘C’ will have 6 (six) essay type or descriptive type questions. Each question will carry 10 (ten) marks and may have two or more parts. Answers are required to be written in the space provided below the question. In no case extra sheets will be provided. All questions in parts ‘A’ ‘B’ and ‘C’ will be compulsory. "

Syllabus

Unit 1: General Bacteriology

Miestones in the development of microbiology, Classification and nomenclature of bacteria. Structure, function and chemistry of bacterial nuclear apparatus. Cytoplasm, Intracellular granules, Cell wal,. Cytoplasmic membrane, Mesosomes, Capsule, Flagella, Fimbrae, Endospore, Protoplasts, Spheroplasts, L-forms, Involution forms. Bacterial stains, staining and microscopy. Growth and nutritional requirements of bacteria. Bacterial enzymes. Respiration in bacteria. Carbohydrate protein, fat and nucleic acid metabolism in bacteria. Reproduction and growth phase of bacteria. Effects of chemical and physical agents and antibiotics. Bacterial variations including transduction, transformation and conjugation. Bacterial vaccines and toxins.
The role of microbial toxins in the pathogenesis of diseases; Biochemical and biological characteristics of toxins produced by various bacteria. Toxin producing Gram-positive and Gram-negative bacteria. Properties and clinical conditions produced by different bacterial toxins.

Unit 2: Systematic Bacteriology

Systematic study of bacteria belonging to genera Borrelia, Leptospira, Campylobacter, Pseudomonas, Brucella, Bordetella, Escherichia, Citrobacter, Salmonella, Shigella, Klebsiella, Enterobacter, Proteus, Vibrio, Haemophilus, Staphylococus, Streptococcus, Bacillus, Clostridium, Listeria, Erysipelothrix, Corynebacterium, Nocardia, Rickettsia, Chalmydia, Mycoplasma, Acholeplasma, Spiroplasma, Anaeroplasma and Thermoplasma, Rhodococcus, Mycobacterium and Neisseria.

Unit 3: General Virology

Historical development of virology. Evolution, classification and nomenclature of viruses. Biophysical and biochemical characteristics of viruses. Cultivation of viruses and their growth pattern in cell culture, embryonated eggs and experimental animals. Purification and concentration of viruses. Qualitative and quantitative assay of viruses. Viral replication. Virus-host cell relationships. Replication strategies of animal viruses and molecular pathogenesis for selected viral system. Latent, persistent and chronic virual infections. Study of genetic variability of animal viruses through use of monoclonal antibodies, autoimmunity, immunosuppression and viral mutation in persistence infections. General principles of laboratory diagnosis of viral diseases. Epidemiology and pathology of viral ifections. Immune mechanism in viral diseases. Interference and interferon. Viral vaccines, point of action of antiviral molecules during the replication cycle of a virus and search for new antiviral compounds, viruses and gene therapy. Chemotherapy of viral infections.

Unit 4: Systematic Virology

Systematic study of RNA and DNA viruses in livestock and poultry with reference to antigenicity, cultivation, pathogenesis, epidemiology, diagnosis and immunity, RNA viruses: Retroviruses and lymphotropic viruses, Visna and Maedi, Arboviruses, Rotaviruses. Birnaviruses. Picornaviruses. Bunyaviruses. Cornoaviruses. Togaviruses, Paramyxoviruses, Orthomyxoviruses, Rhabdoviruses. Picornaviruses. Bunyaviruses. Arenaviruses. Arterioviruses. Calciviruses. Filovirsuses, DNA viruses: Poxviruses. Hepadnaviruses. Iridoviruses, Adenoviruses, Papova viruses. Paravo viruses. Hepadanaviruses. Circoviruses. Unclassified viruses. Slow viruses Scrapie.

Unit 5: Mycology

General characterstics of fungi. Classification and study of pathogenic fungi- Epidermophyton, Microsporum, Trichophyton, Cryptococcus, Aspergillus, Blastomyces, Coccidioides, Histoplasma, Candida, Rhinosporidum, Contaminating fungi, Rhizopus, Mucor and Penicillium. Fungi causing mastitis, abortion and mycotoxicosis.

Unit 6: Immunology

Historical Perspectives. Host-parasite relationships. Antigens. Types of antigens. Properties and specificity of antigens. Factor determining antigenicity. Haptens and carriers. Heterophile antigens. Adjuvants. Mechanisms of actiom classification and their uses. Immunoglobulins - their classes and sub-classes, structure and function. Allotypes. Idiotypes. Genes coding for Igs. Generation of diversity. Monoclonal antibodies. Purification of antibodies. Theories of antibody formation. Lymphoid organs: primary, secondary and circulation of lymphocytes, ce Is involved in the immune response - B lymphocytes, T lymphocytes, subsets and nature of receptors. Macrophages, Dendritic reticular cells, Langerhan’s cells. Cellular interactions Cell- mediated immune responses. Mechanism of interaction of antigen and antibody. The complement system. Classical and alternate pathways. Serological reactions: agglutination, precipitation, neutralization, CFT, FAT, ELISA, DIE, RIA, etc, Immunological methods as an aid to diagnosis, blotting techniques like Northern, Western blotting, Major, Histocompatibility complex: organization.
Nature of antigens and MHC restriction. Hypersensitivity - immediate and delayed types, and mechanism of hypersensitivity. Mechanism of immunity, autoimmunity and immunological tolerance.

Unit 7: Molecular Cell Biology (Vaccine & Diagnostic Technology)

Role of biotechnology in diagnostics and vaccines, RNA electropherotyping. Probes - preparation of cDNA. Use of DNA probe in animal diseases diagnosis. Monoclonal antibodies. Application on monoclonal antibodies for diagnosis of animal diseases. Preparation of monoclonal antibodies. Nucleic acid hybridization Modern trends in vaccines. Recombinant DNA vaccines and their probable use in animal diseases. Bioinformatic tools in microbial research, Biosafety, Biosecurity, GMP and GLP.

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