eChapters

INTRODUCTION

Banana (Musa species) also known as ‘Apple of Paradise’ is an important and commercial fruit crop of tropical regions of the world. It is the only tropical fruit traded to the extent, that it is the 5th largest agricultural commodity after cereals, sugar, coffee and cocoa in world market.

The word ‘banana’ is now applied to all table varieties. Formerly, it was applied to ‘Nendran’ banana of Kerala which was however called ‘Plantain’ by Westerners. The English used the word ‘Plantain’ to represent cooking banana while in parts of South India, even sweet fruited clones were called ‘Plantains’. The variant with persistent bracts and male flowers with large fruits held in lax manner and eaten after cooking were grouped under plantains. All other types which had small fruits, held compact in a bunch with softening of the pulp when ripe, dropping of withered bracts and staminate flowers as the banana. In other words plantain are starchy, not sweet and cooked as vegetable. They usually resembles green bananas, are longer and have thick skin. Bananas are sweet, shorter and have thinner skin. They are eaten as a fruit and have yellow colour when ripe.

14.1. INTRODUCTION

Barley (Hordeum vulgare L. emend. Bowden) is one the most important ancient crops and played very important role in the development of agriculture and human civilization. It has also played crucial role in the progress of science of agriculture, physiology, genetics, plant breeding and malting and brewing science. It is the fourth most cereal crop after rice, wheat and maize in acreages and production in the world.  It is grown and used all around the world in many countries and has fed livestock, poultry and people since ancient time.  Barley was domesticated about 10,000 years ago in the Fertile Crescent of the Near East. Fertile Crescent is considered the first of at least seven primary centres of agriculture origin in the world (Smith, 1998; Baik and Ulrich, 2008). Barley is best known around the world as a feed grain and a premier grains for malting and brewing. When most people think of composition of beer, they only think of barley over other cereals. Although utilization of barley for food is relatively low as compared to other cereals today, throughout its history barley has remained an important and major food source poor people living in Western and Eastern Asia and Northern and Eastern Himalayan region nation like Tibet, Nepal and in Northern and Eastern Africa (Newman and Newman, 2006, 2008). Moreover, there has been resurgence of interest and use of barley as food primarily in the developed world due to an increasing emphasis on incorporating the diversity in consumption of whole grain in people’s diets due to its health benefits.

Natural Gene Flow in Plants

More than 70% of plant species, for example potato, oat, rape-seed, wheat, and kiwifruit have come from spontaneous interspecific or intergeneric hybridization although the extent of natural hybridization differs among the plant genera and families. It creates genetic variation and produce recombinant genotypes that may exhibit heterosis.The stabilization of these hybrids often through polyploidy (autopolyploidy or allopolyploidy) can result in the formation of new biological species (Masterson, 1994), thereby, having impact on both ecological and evolutionary processes (Martinsen et al., 2001). In addition, species genomes are often differentially permeable to introgression if they are under simple genetic control (unlike many genes contribute to hybrid fitness with linkages) and certain portion of the genome can readily incorporate alien alleles. Introgression is a selective process of gene flow that filters deleterious genes and takes only beneficial genes (incorporating the alleles from one taxon into other).

Abstract

Beekeeping is an agro-based activity which is being undertaken by farmers/landless labours in rural area as an integrated framing practice. It supplements income & employment generation and nutritional intake. Honeybees have been offering services to the society through ensured pollination in crops and providing honey and a variety of other bee hive products viz.; royal jelly, pollen, propolis, bee wax, bee venom, etc. Honey Bees have their vital role in sustaining plants bio-diversity resulting in environmental stability. Mainly five species of honeybees viz.; Apis cerana, A. mellifera, A. florea, A. dorsata, Stingless bees are in used in India.  Beekeeping industry has quadruple benefits: 1) providing self employment to rural and forest based population; 2) production of honey, pollen and beeswax, venom, royal jelly, etc., 3) providing employment to rural educated youths in collecting, processing and marketing of bee/beehive-products; and 4) cross-pollination of various agricultural and horticultural crops and improving their quality and increasing yields.

A gene microarray is a (mxn) matrix, containing n-experimental conditions, also called attributes for m-genes. Gene clustering refers to partitioning a microarray of genes based on the commonality of attributes. Genes belonging to a cluster often do not coexpress or coregulate. This calls for biclustering that segregates the (mxn) matrix into small windows. In other words biclustering refers to simultaneous clustering of rows and columns of the microarray. This chapter reviews some of the well known biclustering algorithms, and proposes an alternative solution to the problem using evolutionary multi-objective optimization technique.

Introduction

Clustering is process to automatically partition a set of p-dimensional data points (p³1) into classes, where the data in each class have similarity with respect to a given distance metric. Each class usually has an ideal member that possesses the necessary characteristics of the class.

Abstract

The bio-drainage system used to control the water logging hazards and restore the ground water table depth at a level favourable for other crops to grow. The water utilization efficiency per unit area of land improved through bio-drainage and associated cropping activities. The huge quantity of shallow and good quality groundwater that remains perpetually unutilized by the prevalent lowland cultivation system was effectively used by the biological drainage system. Thus the bio-drainage is expected to alleviate the land degradation problem due to high depth of water stagnant, to restore the crop cultivation programme on a sustainable way, to improve the water management practices, to improve the economic conditions of the lowland farmers and finally, to conserve the ecological values of this fragile agro-ecosystem.The objectives of efficient bio-drainage system are to evaluate ET and depended on growth characteristics of different trees and vegetation species having bio-drainage potential in the waterlogged land thereby to study the water table behaviour in the land under plantation and in the surrounding region and use the result in developing suitable plantation geometry to ensure desired water table draw-down, to assess interaction between bio-drainage plantation and co-existing crops and/or companion crops, to assess the value of the tree species through fodder, fuel wood and timber use, and to development of a new technology for land reclamation and evaluating its impact on soil, water and plant bodies in the basin.

INTRODUCTION

Insects are the major group of arthropods and the most diverse group of animals across the Earth. Though some of them are beneficial to environment and to humans, many insects are considered as pests as they transmit diseases and damage the crops and forests. Chemical, biological, cultural and mechanical methods are being used to eradicate, control or manage these pests. The main strategy used for controlling pests has been the use of chemical pesticides because of their long residual action and wide spectrum toxicity (Casida and Quistad, 1998). However, the indiscriminate usage of chemical pesticides are toxic to mammals, birds and fish, also pollute the environment, besides the development of pest resistance and the emergence of secondary recalcitrant insect pest populations (Li et al., 2007). These negative impacts prompted the change of strategies in managing the pests by resorting to ecofriendly and cost effective pest control strategies (Ferre et al., 1991). 

Abstract
 
Adoption of agricultural practices is cost effective bioremediation techniques to mitigate arsenic problems and can contribute substantially to the cause of removal of arsenic and other heavy metal contamination of soils and water thereby quality enrichment in marketable agricultural produce leading to increased crop productivity in the area of intensive cropping under irrigated eco system. Though the farmers are already practicing some traditional techniques to mitigate the arsenic problems in soils and water, like cultivation of crops which are less affected to arsenic and heavy metals pollution, growing hyper accumulating crops and so many practices wherein the modern methods are not prevalently used by the farmers due to the lack of scientific validity of these techniques, particularly in the context of prevailing socio-economic conditions. Moreover, acceptance of these improved practices by the small farmers of this region is an evolutionary process and should be intensively proved. Hence, extensive studies need for bio or phyto-remediation of heavy metals including arsenic contamination for quality marketable agricultural produce, which ultimately can realize better crop productivity.

1. Introduction

The growing prevalence of obesity and obesity associated disorders as well as its impact on public health has received increased attention in recent years. As a result, obesity is now considered one of the world’s leading public health problems. A study published in the noted medical journal Lancet says India is just behind US and China in this global hazard list of top 10 countries with the highest number of obese people. The study - titled ‘Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: A systematic analysis for the Global Burden of Disease Study 2013’ - used data collected by international bodies and organizations in various countries like India over three decades. China and India together accounted for 15 per cent of the world’s obese population, with 46 million and 30 million obese people, respectively. According to the study, the number of overweight and obese people globally increased from 857 million in 1980 to 2. 1 billion in 2013. This is one-third of the world’s population.

With lifestyle disorders forcing more and more people to reel under excess body weight, even relatively younger people are developing joint disorders and knee pain. Excessive weight is associated with a series of health problems, including blood pressure, diabetes, and cardiovascular ailments. Therefore there in an urgent need to envision effective anti-obesity strategies through effective interventions.

Introduction

The organic matter added to soil externally as FYM, crop residues, leaf fall, root deposition etc. has been a part and parcel of Indian agriculture. The total carbon added to soil is subject to microbial decay and slowly the major portion of it finds way back to the atmosphere. There is every possibility that atmospheric CO₂ concentration will increase in near future; that will further lead to increased attention of scientific community to make soil a possible sink for atmospheric CO2. when the carbon sequestration is thought with the perspective of reducing carbon dioxide emission for healthy atmosphere at one hand and improving soil health on the other, there is utmost need to opt for technologies that can fulfill both these objectives. There is growing interest in the use of charcoal or ‘biochar’ to sequester carbon in soil and improve soil fertility (Lehmann and Joseph, 2009). There are many types of soil additives and fertilizers used to improve the structure and functions of soil. Biochar has re-emerged as an issue in the last ten years as everyone is looking for sustainable ways to improve soils and decrease use of chemical fertilizers. 

1.     Determination of lmax
    
2.     Verification of Beer-Lambert Law
    
3.     Estimation of Carbohydrate by Anthrone Method
    
4.     Estimation of Reducing Sugar by Di Nitro Salicylic (DNS) Acid
    
5.     Estimation of Protein by Biuret Method
    
6.     Estimation of Protein by Lowry’s Method
    
7.     Estimation of Protein by Bradford Method
    
8.     Estimation of Cholesterol
    
9.     Estimation of DNA
    
10.     Estimation of RNA
    
11.     Determination of iso Electric Point of Amino Acid

1.     Common Solvents
    
2.     Common Buffer Compositions

1.     Chromatography
    
2.     Identification of Sugar in Fruit Juice by Thin Layer Chromatography
    
3.     SDS-PAGE
    
4.     Composition for Native PAGE
    
5.     Silver Staining of Protein Gels

The different biochemical values serve as an indicator of the correct health status, viz important organ functions,  the alteration in values if any, helps in identifying the specific organ damage and exact diagnosis of the  abnormal health condition or disease. Some of the important biochemical values of the animals, which may assist in diagnosing the specific disease  conditions are listed in this chapter.

ABSTRACT
Methanogens are primarily anaerobic Archaebacteria representing the most primitive earthly creatures, alive since about 3.5 billion years. While oxygen inhibit their activity, they adapt well in a variety of ecological niche, viz., the intestinal tracts of insects and animals including ruminants, sewage digesters, groundwater and deep soil/water, etc. They are involved in biomethanation that converts organic polymers, including the complex recalcitrant lignocelluloses, to CH₄ and CO₂. Enhancing the process by optimising the physical, chemical and molecular parameters is an interesting biotechnology. Molecular research is rapidly progressing on the genomics, gene and its regulation, and methanogenesis as it expresses. Identifying novel species expressing methanogenesis and manipulating the genetic make-up including through cloning in recent years will set goals and leads for future investigations. The biomethanation enzyme Methyl-CoM-reductase (Mcr) constitutes about 10% of the methanogen protein. Due to its abundance and significance, elucidating its structure and its synthesis and regulation mechanism has received much attention recently. Mcr-coding genes from methanogens have been cloned and sequenced. An approach at the molecular level to enhance biomethanation seems to lie in metagenomics. The microbiology, biochemistry, the roles of the participating microbes, their molecular biology and applications in methanogenesis are discussed in this chapter.

ABSTRACT
Biomethanation comprises of four interdependent stages in series, hydrolysis, acidogenesis, acetogenesis and methanogenesis. Methanogenesis, the final stage of biomethanation, is accompanied with the reduction of substrates that include organic acids such as CH₃COOH, and others such as CO₂ and CH₃OH to CH₄ by methanogenic and methanotrophic archaea, usually under anaerobic conditions. Strictly anaerobic methanogenic archaea are found in the ruminant intestine, sewage digester, groundwater and deep soil/water etc. In methanogenesis, H₂ acts as the electron donor while substrates CO₂, CH₃OH and probably CoM-S-S-CoB (for CH₃COOH) act as terminal electron acceptors and reduce to methane, a process known as anaerobic respiration. This conversion is associated with the electrogenic proton motive force along with sodium motive force to drive the ATP synthesis by A0A1 ATP synthase, known as respiratory phosphorylation. The mechanism of conversion of methane from CO₂ by methanogens with cytochromes is same as those without cytochromes except that the reduction of heterodisulphide CoM-S-S-CoB is performed by the membrane-bound VhoACG-HdrDE complex for former while by cytoplasmic MvhADG–HdrABC complex for the later.

Introduction

The current population of our country is about 1.27 billion and the world population is predicted to grow from 6.9 billion in 2010 to 8.3 billion in 2030 and to 9.1 billion in 2050. By 2030, food demand is predicted to increase by 50% (70% by 2050). The main challenge facing the agricultural sector is not so much growing 70% more food in 40 years, but making 70% more food available on the plate (UNDESA, 2014). This rising food demand automatically put burden on the shoulder of formers especially for ASEAN countries such as India where population growth rate is high while resources are limited. India has about 141 million hectares area under cultivation out of its geographical area which 329 million hectares (Raghuwanshi, 2012; Parikh and James, 2012). Consequently, agriculture is, thus, one of the most important factors contributing to the economic growth of India (Kniivila, 2007). The production of the agricultural produce should be up to the mark to maintain this contribution. In order to enhance the crop production, fertilizers are needed because they supply the nutrients to the soil. By adding fertilizers, crop yields can often be doubled or even tripled.

ABSTRACT
Recent challenges for fossil fuels, associated environmental issues with disposal of organic garbage, and the rising costs for energy has encouraged researchers to search for alternate energy sources. Massive uncontrolled biogas from landfills, sludge and organic material degradation under anoxic conditions is an environmental management issue. Methane emission to atmosphere has a tremendous green house effect, but this can also be a valuable renewable energy resource. Biogas from kitchen wastes through anaerobic digestion by methanogens is such an alternate technology. Kitchen wastes are considered one of the best raw materials for biogasification. Biogas is mainly composed of 50-74% methane (CH₄), 25-28% carbon-di-oxide (CO₂), other volatile compounds including hydrogen sulphide (H₂S), water vapour and other trace gas compounds. The impurities in it, like Hydrogen sulphide (H₂S) which is corrosive, downgrade its quality. Purified biogas of higher calorific value attracts attention as a substitute fuel for additional applications. Its purification using various suitable scalable and economically-viable scrubbing technologies to enhance its quality and performance as an alternate fuel is an important step in this direction. Thus, a technology to generate and purify biogas from kitchen waste has significant prospects in the energy sector, and also as a promising enterprise to employ the youth.

ABSTRACT
There is a quantum global growth in both number and size of dairy industries in recent times. These industries discharge thousands of liters of wastewater characterised by a heavy chemical oxygen demand (COD), biological oxygen demand (BOD), nutrients, inorganic salts, besides detergents and sanitisers each day (Kushwaha et al., 2011). High carbohydrates, fats and proteins contents in such waste streams originating from milk pose challenge to conventional municipal sewage treatment systems (Demirel et al., 2005). The easily biodegradable dairy effluent leads to precipitation of casein which is toxic and has a strong odour. Without adequate treatment, these effluents could pose serious threats to the receiving water bodies and disturb the ecosystem (Satpathy et al., 2017). For such heavily loaded organic effluents, biogasification through anaerobic digestion seems a promising strategy. This cost-effective energy generation process is also effective in environmental management compared to its other eco-friendly renewable source counterparts, such as, hydro, solar and wind (Satpathy, 2017). The Upflow Anaerobic Sludge Blanket (UASB) reactors are the most popular anaerobic systems to treat the fluctuating organic loads in dairy effluents. Anaerobic filter (AF), Upflow Anaerobic Filter (UPAF), Fixed-bed anaerobic reactor, Anaerobic Hybrid Reactor (AHR) etc. are few other systems that are demonstrably competent removing COD upto 90% and generating biogas. Successful anaerobic systems reported yielded (with upto about 85% CH₄ content) 0.32-0.34m3 CH₄/kg COD removed. Biomethanation process and reactors designs are constantly being optimised for effective treatment of the effluents while maximising the biogas production. The energy-rich gas thus obtained could further be utilised for heat and electricity at the domestic level, and also as a vehicular fuel. Residual digestate can also be used as organic manures (Angelidaki et al., 2007).

Introduction

Saffron is a naturally derived plant product from the dried stigma of the Crocus sativus flower  (Gresta et al., 2009).The saffron (Crocus sativus L.) is an Iridaceae family member, herbal perennial, sterile (2n=3x=24) geophyte, traditionally propagated by vegetative corms. Its cultivation dates back to late Bronze Age in the Mediterranean region (Negbi, 1999) in Anatolia since the ancient times (Arslan et al., 2007) and for at least 3500 years in Egypt and Middle East. Saffron contains more than 150 volatile and aroma yielding compounds. The chemical components mainly include carotenoids, crocin, picrocrocin, safranal etc (Abdullaev, 1993, Tarantalis et al., 1995, Karaoglu 
et al., 2007). Saffron is mostly used as spice, food colorant, dye or perfume. The dried stigmas introduce unique color, taste and fragrance and thus making it the world’s most expensive spice (Baghlian et al., 2010). Saffron has been used in folklore herbal medicines for centuries (Basker and Negbi, 1983).

Bioinformatics is a recently emerged frontier discipline, which helps in analyzing biological data with the help of information technology and statistical software packages. In a broader sense, bioinformatics is the application of computer technology in management of biological information. This interdisciplinary scientific tool having no barriers between different branches of science like biology, biotechnology, mathematics, computer science and information technology, has great influence on advanced bio-science research, particularly dealing with genes, proteins and cell biology.

Bioinformatics is the branch of study that deals with the use of information technology (IT) for managing data of biological science. Bioinformatics drew public attention with the announcement of the draft sequence of human genome by the then US President Bill Clinton along with Craig Venter and Francis Collins in 2000 (Uma and Sathyanathan, 2004).

Since the dawn of the Industrial Revolution, mankind has been introducing numerous hazardous compounds into the environment at an exponential rate.  Environmental pollutants have become a major global concern. The modern growth of industrialization, urbanization, modern agricultural development, energy generation, have resulted in indiscriminate exploitation of natural resources for fulfilling the human desires and need, which have contributed in disturbing the ecological balance on which the quality of our environment depends.
 
Technological revolution has brought new changes in products and processes in industry. The waste generated from the development of products and processes are of concern to the environmental list. A variety of pollutants such as heavy metals, xenobiotics, polycyclic aromatic hydrocarbons (PAHs), chlorinated and nitro-aromatic compounds are found to be highly toxic, mutagenic and carcinogenic for living organisms (Zhang and Bennett. 2005; Samanta et al., 2002).

Bioinformatics is a multidisciplinary approach that combines computational and biological expertise to analyze biological data to advance research and development and it drew public attention with the announcement of the draft sequence of human genome by the then US President, Bill Clinton along with Craig Venter and Francis Collins in 2000. Bioinformatics and life sciences research are gaining attention in the government, industries and academic sectors. India was the first country in the world to establish a biotechnology information system (BTIS) network in 1987 through the Dept. of Biotechnology and created an infrastructure that enables it to harness biotechnology through the application of bioinformatics. Through this branch of science, biological data are acquired, stored and then analyzed. Bioinformatics can be used to store huge bioscience data for their future uses. By exploring computational tools, databases and new strategies for pests and diseases, resistant genes can be identified and plants with desired traits can be engineered to enhance agricultural productivity. Bioinformatics can be utilized in two ways for agriculture, viz., (i) at molecular level for application in breeding strategies and (ii) agri-informatics for land use planning, farmers window, early warning system, impact assessment and environmental applications.

ANTIBIOTICS

Antibiotic is a drug used to treat infections caused by bacteria and other microorganisms. Technically an antibiotic is a substance produced by a microorganism that selectively inhibits the growth of another. Penicillin was the first antibiotic discovered by Alexander Fleming in 1928, a significant breakthrough for medical science. Antibiotics had saved thousands of wounded soldiers in the I and II world wars. Also, use of it made surgery safer and increased the average life expectancy of human beings. Antibiotics are among the most frequently prescribed medications in modern medicine. Some antibiotics are ‘bactericidal’, meaning that they work by killing bacteria. Each type of antibiotic affects different bacteria in a different way. Some antibiotics can be used to treat a wide range of infections and are known as ‘broad-spectrum’ antibiotics. Others are only effective against a few types of bacteria and are called ‘narrow-spectrum’ antibiotics.

In recent years, we have witnessed an explosion of biological information and the bioinformatics data arising from the rapid research and unprecedented progress in molecular biology. The novel genomic technologies have revolutionized the drug designing, discovery and development processes in government as well as private pharmaceutical industries through the use of these technologies. Various databases are doubling in size every year and fortunately we have the complete genome sequences of more than 100 organisms. At present, the pharmaceutical industries have clasped genomics as a source of drug targets. They also distinguish that the field of bioinformatics is crucial for validating these potential drug targets and for determining the ones that are most suitable for entering the drug development pipeline. Due to our increased understanding of molecular biology, there has been a change in the way that medicines are being developed. Once upon a time, new synthetic organic molecules were tested in animals or in whole organ preparations. Now this approach has been replaced with a molecular target in which in-vitro screening of compounds against purified, recombinant proteins or genetically modified cell lines is carried out with a high throughput as a consequence of better and ever improving knowledge of the molecular basis of disease.

Bioinformatics can be defined as the application of computer technology for the management and analysis of biological data using computer to gather, store, analyze and merge the biological data. It consists of the organization of many kinds of large-scale biological data, particularly that based on DNA and protein sequence, into web-accessible databases and the methods required analyzing these data. Bioinformatics is a fast growing field within the biological sciences that was developed because of the need to handle large amounts of genetic and biochemical data. The goal of bioinformatics is to uncover the wealth of biological information hidden in the mass of data and to obtain a clearer insight into the fundamental biology of organism. This knowledge can have profound impact on various fields such as human health, agriculture, environment, energy and biotechnology. Bioinformatics involves use of various biological databases, which are archives of consistent data that are stored in a uniform and efficient manner. Biological databases are large collections of data that are relatively different to maintain outside the centers and institutions that produce them. These data are traditionally accessed and analysed using browser-based World Wide Web (www) interfaces. There are two types of databases i.e. Primary database and Secondary database. Primary databases contain information and annotations of DNA and Protein sequences, DNA and Protein structure and DNA and Protein expression profiles catalogued for the end users.

1.    Biological Control of Insect Pests
    
2.    Biocontrol Agents in Vegetables
    
3.    Mass Multiplication of Predators and Parasites
    
4.    Microbial Control
    
5.    Transgenic Plants
    
6.    Biological Control of Diseases
    
7.    Conclusion
        
References

Introduction
 
Natural control strategies that employ biological agents for pest suppression are generally classified as biological control tactics. In conventional usage, this term usually refers to the practice of rearing and releasing natural enemies:   parasites, predators, or pathogens. A slightly broader definition of “bio-control” includes any related management activity that is designed to protect or conserve natural enemies. Biological control is the use of living organisms to maintain pest populations below damaging levels. Natural enemies of arthropods fall into three major categories: predators, parasitoids, and pathogens (Altieri et al., 2005; Mahr et al., 2008).

Abstract

Biological drainage relies on vegetation, rather than engineering mechanisms to remove excess soil water through evapo-transpiration. It is often considered attractive because it requires only an initial investment in site development (planting of a “bio-drainage crop”) and (potentially) returns a benefit when the bio-crop is harvested for fodder, wood or fibre. Presently about one-third of the world’s irrigated area faces the threat of waterlogging. Considering long-term viability of bio-drainage, the systems would be subjected to the same scrutiny as other plant-based biological systems with regards to nutrient and salt-balance and soil considerations. Moreover, large-scale adoption of bio-drainage crops could include considerable tracts of land and socio-economic considerations.

1    Biological Hazards
    1.1    Bacterial hazards
    
2.     Fungal Hazards
    
3.    Viral Hazards
    3.1     Hepatitis A virus
    3.2       Norwalk virus
    
4.    Parasitic Hazards
    4.1     Parasitic worms
    4.2    Protozoan parasites
    
5.    Aquatic Biotoxins
    5.1    Paralytic shellfish poisoning (PSP)
    5.2    Diarrheic shellfish poisoning (DSP)
    5.3    Amnesic shellfish poisoning (ASP)
    5.4    Ciguatera fish poisoning (CFP)
    5.5     Puffer fish (Tetrodotoxic) poisoning (PFP)
    
6.    Pathogen Survival Through Cooking
    
References

ABSTRACT
Rice straw is one of the most abundant lignocellulosic agricultural wastes in India and can be used as a major resource for biogas production. The structure of rice straw is complex and is made up mainly of cellulose, hemicelluloses and lignin. The lignin physically shields the cellulose and hemicelluloses, the main components which can be converted to energy, and hinders the access of microbial enzymes to these carbohydrate moieties. To overcome this making the straw a potential resource of biogas production, effective pretreatment is necessary that can efficiently hydrolyse the straw and make it accessible to cellulolytic enzymes and methanogens. Various pretreatment methods which are employed currently in the market are discussed here. For a process to be economically feasible it is necessary that the pretreatment be efficient as well as cost effective. To gain some insights into this aspect, the techno-commercial feasibility of Biomethanation of rice straw has been discussed.

ABSTRACT
Nisargruna biogas technology has been developed in BARC for processing different types of biodegradable waste. This technology is based on biphasic separation of aerobic and anaerobic stages. Introduction of aerobic predigester has increased the efficiency of the process in terms of enhancing the quality and quantity of methane and also reducing the retention time of the process. Process parameters of the plant were studied and found that the modified design has proved very effective in faster digestion of complex waste. The technology has been commercialised and has spread across different parts of country for processing variety of wastes including kitchen waste, municipal waste, agriculture waste, textile waste and poultry waste. Microbial population from different stages of the plant was also studied and found their ability to convert waste to biogas, containing mainly methane.

Keywords: biogas, biphasic digestion, green energy, methane, microbial fermentation, Nisargruna, waste management

The successful AI program depends on the health of the semen donors followed by hygiene in semen collection, processing, storage and AI methods as well as reproductive health of the females. However, the microbial contamination may not be able to avoid completely but it can be reduced to the minimum threshold level by adopting hygienic procedures.

The hygienic condition of the neat / frozen semen samples can be assessed periodically by enumerating the bacterial load apart from the periodical screening of bucks for the presence of various causative agents in animals and its transfer to the females via semen samples.

Though both viable and total (viable and nonviable) bacterial counts can be done, the viable techniques are more commonly used in diagnostic and food hygiene procedures. The viable bacteria are capable of multiplication with the production of visible colonies on or in agar media. In viable techniques, the assumption is made that one well-spaced bacterial cell gives rise to one colony. The bacterial colonies, rather than bacterial cells, are counted in these techniques. Among the viable counting techniques like Spread plate, Pour plate, Miles, Misra, Filtration are available and each has its own inherent errors, the pour plate counting method is explained as follows:

Introduction

Agriculture is one of the promising sectors which offers marvelous potential for sustainable growth and play a key role in the economy of the country. Many of the foods that are already common in our diet are obtained from plant varieties that were developed using conventional genetic techniques of breeding and selection. Increase in the world population and limited food supply strained researchers to introduce and advance agriculture management and modern technologies along with conventional practices to attain highest possible crop productivity. Agricultural technology is based on the domestication of wild plants to create the crops that we have come to depend upon. Humans invented agriculture approximately 10,000 years ago when they began to harvest and cultivate specific plants to produce food. The improved plant traits selected by early agriculturalists were transmitted genetically to succeeding generations of plants. Both public institutions and private companies are now involved in crop genetic improvement.

Crop yields are affected by a combination of abiotic stresses, biotic stresses, and nutritional factors but abiotic stresses (drought, heat, cold or salinity) are the major factors that prevent crops from realizing their full yield potential (Edmeades, 2009). In traditional approach, breeders grow and cross varieties and then evaluate how the progenies vary in their ability to deal with stresses. The best-adapted plants are then selected for growing in fields exposed to stresses. Biotechnologists have taken advantage of recent advances in biotechnology and functional genomics to genetically engineer crops which can give better yield in adverse conditions than the unmodified ones (Manavalan et al., 2009; Umezawa et al., 2006). Drought, extreme temperatures (high or low), high salinity and cold are the major abiotic stresses that affect plant growth and result in significant yield losses. Although plants have evolved a wide spectrum of programs for adaptation to changing environment, the current understanding of the mechanisms associated with the ability of crops to maintain yield under abiotic stress are poorly understood (Witcombe et al., 2008; Munns et al., 2008; Bartels et al., 2005). New advances in ‘omic’ technologies are providing opportunities for identification of transcriptional, translational and post-translational mechanisms and signaling pathways that regulate the plant response. This chapter describers several examples of how modern crop technologies may be applied to broaden crop tolerance of various abiotic stresses and to increase total biomass.

Environmental constraints that include abiotic stress factors such as salt, drought, cold, extreme temperatures and heavy metalsalready a major limitingfactor for crop productivity and will soon become even more severe due to climate change conditions.Together, these stresses constitute the primary causes of crop losses worldwide, reducing average yields of most major crop plants by more than 50% (Boyer, 1982; Bray et al., 2000; Wang et al., 2003). Current climate change scenarios predict an increase in mean surface temperatures and drought that will drastically affect global agriculture in the near future (Le Treut et al., 2007).
 
Abiotic stresses trigger many biochemical, molecular and physiological changes and responses that influence various cellular and wholeplant processes (Wang et al., 2001, 2003). For example, drought, salinityand low temperature stress lead to reduced availability of water (alsoknown as dehydration/osmotic stress) characterized by a decreasedturgor pressure and water loss (Dhariwal et al., 1998; Boudsocq and Lauriere, 2005). Osmotic stress promotes the synthesis of the phytohormoneabscisic acid (ABA)which then triggers a major change in gene expressionand adaptive physiological responses (Seki et al., 2002; Yamaguchi and Shinozaki, 2006; Shinozaki andYamaguchi-Shinozaki, 2007).

Introduction

Stress in physics is any force applied to an object. Stress in biology is any change in environmental conditions that might reduce or adversely change a plant’s growth or development. ‘Stress’ or ‘pressure’ was introduced into the theory of elasticity as an amount of force for a given unit area. When sufficient force is applied to material, the material bends and the change in length is termed ‘strain’. With increasing stress, the initially linear relationship between stress and strain becomes nonlinear until the proportionality limit, after which the material deforms elastically (it can bend back), then plastically (it cannot bend back) until it ruptures. Since the 1930s, biologists have attempted to apply this terminology to biological systems, albeit the nature of the stresses will vary between nonliving materials and organisms.

Reproductive Biotechnology
    
Artificial Insemination (AI)
    
History and Future Perspectives of Artificial Insemination
    
Semen Cryopreservation
    
Buffalo
    
Goat
    
Cryoprotection and Anti Oxidants
    
Cryopreservation and Genetic Stability of Livestock Spermatozoa
    
Insemination Technique
    
Cattle and Buffalo
    
Sheep and Goat
    
Conception Rate with Artificial Insemination
    
Multiple Ovulation and Embryo Transfer (MOET)
    
Advantages of Embryo Transfer
    
MOET Includes the Following Steps
    
Superovulatory Treatments
    
Insemination
    
Recovery of Embryos
    
Synchronization of Recipients
    
Cattle
    
Buffaloes
    
Goats
    
Horses
    
In vitro Maturation, fertilization and culture of Oocytes
    
Historical Development
    
In Vitro Maturation of Oocytes
    
In vitro Sperm Capacitation
    
Scope
    
Limitations
    
Embryo Cryopreservation
    
Ultrasonography in Reproductive Management
    
Ultrasound-guided Transvaginal Oocyte Pick-up (OPU)
    
Embryo Cloning or Nuclear Transfer
    
Blastomere Separation
    
Embryo Splitting
    
Nuclear Transfer Cloning (NT)
    
Embryonic Stem Cell Complementations
    
Limitations and Future Prospects of Cloning
    
Sexing of Embryos and Separation of X and Y Chromosomes Bearing Sperms
    
PCR  in Sex Determination
    
Transgenic Animal Production
    
Gene Transfer Through Direct DNA Microinjection
    
Gene Transfer with Retroviral-Vector Assistance
    
Gene Transfer through Production of Germ-line Chimeras
    
Parthenogenetic Embryos: An Emerging Tool in Reproductive Biotechnology for Multiplication of Superior Germ Plasm
    
Applications of Parthenogenesis
    
Livestock Genomics and Marker Assisted Selection (Use of DNA Level Markers)
    
Stem Cells
    
Vaccine for Fertility Control and Increase Prolificacy
    
Auto-immunization to Increase Prolificacy
    
Biotechnology of Conservation of Livestock Breed Biodiversity
    
Gene for Prolificity: Booroola Merino
    
Role of Biotechnology in Enhancing Production
    
Milk
    
Biotechnology of Rumen Manipulation
    
Genetically Modified Ruminal Microorganisms

Biotechnology of Animal Feeding
    
Probiotics
    
Biotechnology Crops as Animal Feed
    
Biotechnology of Animal Products
    
Some New Products made Possible by Biotechnology Include

Biotechnology provides significant opportunities and greater effects in horticulture, where even minor changes such as quality, colour, aroma, flavour, structure, disease-pest resistance and postharvest behaviour bring tremendous achievements commercially. Tissue culture, genetic engineering, molecular breeding, biofertilizers, biopesticides and postharvest biotechnology are important aspects of biotechnology in horticulture such as fruit, vegetable, ornamental, spice, medicinal, aromatic and plantation crops.

Tissue Culture

Tissue culture is the true reality of the plant biotechnology. The annual demand of tissue cultured production constitutes nearly 10 per cent of the total world biotech business, amounting to 15 billion US dollars. The global production of tissue culture industry is expanding @ 15% per annum (Chadha, 2000). In India, more than 130 tissue culture units are presently producing > 300 million plantlets per annum (Choudhury and Tejaswini, 2000).

Animal Science Biotechnology Research Centres
    
Current Status and Some of the Achievements in Animal Heatlh
    
National Bioresource Development Board
    
Animal Biotechnology
    
Biotechnology Parks and Incubators
    
National Institute of Immunology, New Delhi
    
Bose Institute, Kolkata
    
Jawahar Lal Nehru University, New Delhi
    
National Centre For Cell Science, Pune
    
Centre for Dna Fingerprinting and Diagnostics (Cdfd) Hyderabad
    
National Institute for Plant Genome Research (Nipgr) New Delhi
    
Institute of Bioresources and Sustainable Development,Imphal
    
Institute of Life Sciences, Bhubaneshwar
    
Bharat Immunologicals and Biologicals Corporation Limited
    
International Centre for Genetic Engineering and Biotechnolgoy (Icgeb), New Delhi
    
Animal Biotechnology Research Abroad

1. Introduction

Stress-Any deviation from this optimal condition of any factor essential for its growth will lead to aberrant change in physiological processes and due to this plant body will experience tension and this state can be referred as plant under stress.

In physics, stress is defined as mechanical force per unit area applied to an object. In response to the applied stress, an object undergoes a change in the dimension, which is also as strain. However, the biological stress is defined as an advance force or a condition, which inhibits the normal functioning and well-being of a biological system such as plants. There are several abiotic and biotic factors, which under a given condition, may be perceived as stress by plants.

4.1. Substrate quality
    Litter quality
    Soil organic matter (SOM) quality

4.2. Decomposer community
    Soil animals
    Soil microorganisms
    Terrestrial fungi
    Actinomycetes    
    Bacteria

India is the largest producer and consumer of pulses in the world, accounting for 33 per cent area and 25 percent production. Pulses are mostly grown under rainfed conditions in marginal to sub-marginal lands. Once a net exporter it is presently one of the largest importer of pulses as our demand outstrips domestic production. While the traditional cropping pattern almost always included a pulse crop either as a mixed crop or in rotation, the commercialization of agriculture has encouraged the practice of sole-cropping. As a result, the annual import has increased from 0.50 million tonnes to 1.80 million tonnes during the last five years and contribution of pulses in the national food basket reduced from 17% to 7%. Pulses are generally grown on marginal and sub-marginal lands under rainfed conditions with low inputs and suffer heavily due to biotic and abiotic stresses, resulting into low productivity. Challenges for pulses in India are decline in area of pulses, low genetic yield potential, low realized yield, instability in production, climate change, biotic and abiotic stresses, poor seed replacement rate, post harvest losses, wide fluctuation in price, no regular MSP/procurement policy, poor availability of critical inputs in productivity zone and poor transfer of technology.

Vegetable is an important constituent of Indian diet. India is the second largest producer of vegetables which contributes 16.7% to global vegetable area with a production of 156.33 million tons (15.4%) (Table 1). The level of productivity of vegetable17.4 tons/ha is quite low (Rai et al., 2014). Majority of Indians are vegetarian, with a per capita consumption 135 g per day as against the recommended 300 g per day. It is still very less than recommended diet level. In near future, there is a need of around 5-6 million tons of food to feed our 1.3 billion Indian population expected by the year 2020 (Paroda 1999). The major biotic stress in vegetable production is due to increased insect pest. In many cases, there is 100 per cent yield loss due to viral diseases vectored by insects. Vegetables are more prone to insect pests and diseases mainly due to their tenderness and softness as compared to other crops and virtual absence of resistance characters because of intensive hybrid cultivation. The insect pests inflict crop losses to the tune of 10-30 per cent in vegetable production (Table 2). To overcome these losses farmers used indiscriminate doses of toxic chemical on vegetable. It accounts for 13-14 per cent of total pesticides consumption, as against 2.6 per cent of cropped area (Sardana, 2005). For the benefit of the farmer and other, application of BIPM is must. Bio intensive pest management involves every part of management that keeps pest below economic injury level so that pest does not cause any economic loss to the farmers or growers.

Abstract

Abiotic stresses have been a serious challenge for sustainable agriculture particularly vegetable cultivation characterized by decreased endurance against stresses like drought, temperature fluctuation and salinity. In India, drought and salinity being major abiotic stresses affect an area of 16% and 4.28%, respectively. Plant Breeders have to face many impediments while developing stress tolerant varieties. Developing tolerant varieties is quite difficult as tolerance varies with the developmental stages and has complex inheritance. This can be understood well by studying ontogeny of plants. Drought varies in time of occurrence, severity and duration. Field screening for tolerance is very difficult because of various interactions like drought × high temperature, high temperature × salinity interactions etc. Artificial screening in different stresses could be an alternative approach. The varieties developed with higher inputs at research stations fail to perform at farmers’ fields due to high genotype × environment interactions. Cultivated species have less genetic variability for abiotic stress tolerance but tolerance can be enhanced by using diverse and heterogeneous population. Though wild species possess genes for tolerance but these genes are closely linked with the undesirable gene(s) e.g. in tomato where S. cheesmanii, S. peruvianum, S. pimpinellifolium and S. sitiens have undesirable traits which affect yield and quality adversely.

Abstract

Modern agriculture has disturbed the age-old balance of host-pathogen system resulting diseases occur at regular interval leading to enormous loss to the agricultural production, which is estimated to be 10-25% or less during normal years but may extend up to 100 % during epidemic. Broadly, there are two breeding approaches adopted for disease resistance which includes conventional and molecular one. Conventional approach includes pureline, pedigree, backcross, recurrent selection and interspecific hybrids through which various resistant varieties has been developed. However, after sometime there is sudden outbreak of diseases due to continuous sowing of these varieties which results in the advancement in molecular biology and with the discovery of trait linked markers it paved a new pathway to look a new corner to develop varieties which are disease resistant. Molecular approach consists of gene pyramiding through which two or more genes providing resistance to different types of diseases is introgressed in a particular variety, gene deployment and multiline varieties which consists of heterogeneous population formed by mixing the cultivars to differ in the ability of disease resistance and reduces the rate of epidemic development. With the help of makers in marker assisted breeding using foreground, recombinant and background selection several linked markers are identified which provides resistance against disease like in wheat stem rust, leaf rust etc.

Ian Donald in the year 1956 used the one-dimensional A-mode (amplitude mode) to measure the parietal diameter of the fetal head and thus introduced the ultrasound in diagnostic and medicine. Ultrasonography does not use radiation or powerful magnetic field as source of energy and the equipment cost is usually affordable. This modality involves the use of sound waves in viewing the longitudinal, sagittal and transverse planes for both testes . Sound is measured in cycles per second or hertz (Hz); the audible range of sound is from 20-20,000 Hz. Diagnostic ultrasound is usually in the range of 1–20 megahertz (MHz).

The speed of sound depends on the tissues through which it propagates because density and compressibility change the speed, the sound wave travels. On an average the assumed speed of sound in soft tissue is 1540 m/s.

India has a mega animal bio-diversity, which is manifested in the form of availability of a large number of species, and a variety of breeds even within species. India is bestowed with rich and varied domestic animal biodiversity having a total of 32 breeds of cattle and 10 buffalo breeds. The diversity of livestock is not only accidental, nor purely natural; it is more the outcome of thousands of years of continuous selection. The trends in livestock population over the last three decades have shown that, there is significant increase in the population of buffaloes i.e. from 60.5 crore in 1973 to 96.9 crore in 2003, while cattle population has increased from 180.1 crore to 226.1 crore in the same period. The share of buffaloes, in the bovine population increased from 22 to 32 per cent while that of cattle has shown declining trend. Within the cattle, there has been marked shift from work animals towards milch animals which has resulted in loss of important draught breeds of cattle. Further indiscriminate crossbreeding has resulted in significant decline in number of important milch breeds like Sahiwal.

Biological and chemical reactions in living cells are dramatically reduced at low temperature, a phenomenon that can lead to the possible long-term preservation of cells and tissues. However, freezing is fatal to most living organisms, since both intra and extracellular ice crystals are formed and results in changes to the chemical setting of cells that lead to cellular mechanical constraints and injury. The major hurdle for cells to overcome at low temperatures is the water-to-ice phase transition. Cell injury at fast cooling rates is attributed to intracellular ice formation, whereas slow cooling causes osmotic changes due to the effects of exposure to highly concentrated intra and extracellular solutions or to mechanical interactions between cells and the extracellular ice. Cryopreservation is a process that maintains biological samples in a state of suspended animation at cryogenic temperature for any considerable period and is used to preserve the fine structure of cells (Jang et al., 2017).