eBook Chapters

1.     Common Solvents
    
2.     Common Buffer Compositions

1.     Chromatography
    
2.     Identification of Sugar in Fruit Juice by Thin Layer Chromatography
    
3.     SDS-PAGE
    
4.     Composition for Native PAGE
    
5.     Silver Staining of Protein Gels

The different biochemical values serve as an indicator of the correct health status, viz important organ functions,  the alteration in values if any, helps in identifying the specific organ damage and exact diagnosis of the  abnormal health condition or disease. Some of the important biochemical values of the animals, which may assist in diagnosing the specific disease  conditions are listed in this chapter.

ABSTRACT
Methanogens are primarily anaerobic Archaebacteria representing the most primitive earthly creatures, alive since about 3.5 billion years. While oxygen inhibit their activity, they adapt well in a variety of ecological niche, viz., the intestinal tracts of insects and animals including ruminants, sewage digesters, groundwater and deep soil/water, etc. They are involved in biomethanation that converts organic polymers, including the complex recalcitrant lignocelluloses, to CH₄ and CO₂. Enhancing the process by optimising the physical, chemical and molecular parameters is an interesting biotechnology. Molecular research is rapidly progressing on the genomics, gene and its regulation, and methanogenesis as it expresses. Identifying novel species expressing methanogenesis and manipulating the genetic make-up including through cloning in recent years will set goals and leads for future investigations. The biomethanation enzyme Methyl-CoM-reductase (Mcr) constitutes about 10% of the methanogen protein. Due to its abundance and significance, elucidating its structure and its synthesis and regulation mechanism has received much attention recently. Mcr-coding genes from methanogens have been cloned and sequenced. An approach at the molecular level to enhance biomethanation seems to lie in metagenomics. The microbiology, biochemistry, the roles of the participating microbes, their molecular biology and applications in methanogenesis are discussed in this chapter.

ABSTRACT
Biomethanation comprises of four interdependent stages in series, hydrolysis, acidogenesis, acetogenesis and methanogenesis. Methanogenesis, the final stage of biomethanation, is accompanied with the reduction of substrates that include organic acids such as CH₃COOH, and others such as CO₂ and CH₃OH to CH₄ by methanogenic and methanotrophic archaea, usually under anaerobic conditions. Strictly anaerobic methanogenic archaea are found in the ruminant intestine, sewage digester, groundwater and deep soil/water etc. In methanogenesis, H₂ acts as the electron donor while substrates CO₂, CH₃OH and probably CoM-S-S-CoB (for CH₃COOH) act as terminal electron acceptors and reduce to methane, a process known as anaerobic respiration. This conversion is associated with the electrogenic proton motive force along with sodium motive force to drive the ATP synthesis by A0A1 ATP synthase, known as respiratory phosphorylation. The mechanism of conversion of methane from CO₂ by methanogens with cytochromes is same as those without cytochromes except that the reduction of heterodisulphide CoM-S-S-CoB is performed by the membrane-bound VhoACG-HdrDE complex for former while by cytoplasmic MvhADG–HdrABC complex for the later.

Introduction

The current population of our country is about 1.27 billion and the world population is predicted to grow from 6.9 billion in 2010 to 8.3 billion in 2030 and to 9.1 billion in 2050. By 2030, food demand is predicted to increase by 50% (70% by 2050). The main challenge facing the agricultural sector is not so much growing 70% more food in 40 years, but making 70% more food available on the plate (UNDESA, 2014). This rising food demand automatically put burden on the shoulder of formers especially for ASEAN countries such as India where population growth rate is high while resources are limited. India has about 141 million hectares area under cultivation out of its geographical area which 329 million hectares (Raghuwanshi, 2012; Parikh and James, 2012). Consequently, agriculture is, thus, one of the most important factors contributing to the economic growth of India (Kniivila, 2007). The production of the agricultural produce should be up to the mark to maintain this contribution. In order to enhance the crop production, fertilizers are needed because they supply the nutrients to the soil. By adding fertilizers, crop yields can often be doubled or even tripled.

ABSTRACT
Recent challenges for fossil fuels, associated environmental issues with disposal of organic garbage, and the rising costs for energy has encouraged researchers to search for alternate energy sources. Massive uncontrolled biogas from landfills, sludge and organic material degradation under anoxic conditions is an environmental management issue. Methane emission to atmosphere has a tremendous green house effect, but this can also be a valuable renewable energy resource. Biogas from kitchen wastes through anaerobic digestion by methanogens is such an alternate technology. Kitchen wastes are considered one of the best raw materials for biogasification. Biogas is mainly composed of 50-74% methane (CH₄), 25-28% carbon-di-oxide (CO₂), other volatile compounds including hydrogen sulphide (H₂S), water vapour and other trace gas compounds. The impurities in it, like Hydrogen sulphide (H₂S) which is corrosive, downgrade its quality. Purified biogas of higher calorific value attracts attention as a substitute fuel for additional applications. Its purification using various suitable scalable and economically-viable scrubbing technologies to enhance its quality and performance as an alternate fuel is an important step in this direction. Thus, a technology to generate and purify biogas from kitchen waste has significant prospects in the energy sector, and also as a promising enterprise to employ the youth.

ABSTRACT
There is a quantum global growth in both number and size of dairy industries in recent times. These industries discharge thousands of liters of wastewater characterised by a heavy chemical oxygen demand (COD), biological oxygen demand (BOD), nutrients, inorganic salts, besides detergents and sanitisers each day (Kushwaha et al., 2011). High carbohydrates, fats and proteins contents in such waste streams originating from milk pose challenge to conventional municipal sewage treatment systems (Demirel et al., 2005). The easily biodegradable dairy effluent leads to precipitation of casein which is toxic and has a strong odour. Without adequate treatment, these effluents could pose serious threats to the receiving water bodies and disturb the ecosystem (Satpathy et al., 2017). For such heavily loaded organic effluents, biogasification through anaerobic digestion seems a promising strategy. This cost-effective energy generation process is also effective in environmental management compared to its other eco-friendly renewable source counterparts, such as, hydro, solar and wind (Satpathy, 2017). The Upflow Anaerobic Sludge Blanket (UASB) reactors are the most popular anaerobic systems to treat the fluctuating organic loads in dairy effluents. Anaerobic filter (AF), Upflow Anaerobic Filter (UPAF), Fixed-bed anaerobic reactor, Anaerobic Hybrid Reactor (AHR) etc. are few other systems that are demonstrably competent removing COD upto 90% and generating biogas. Successful anaerobic systems reported yielded (with upto about 85% CH₄ content) 0.32-0.34m3 CH₄/kg COD removed. Biomethanation process and reactors designs are constantly being optimised for effective treatment of the effluents while maximising the biogas production. The energy-rich gas thus obtained could further be utilised for heat and electricity at the domestic level, and also as a vehicular fuel. Residual digestate can also be used as organic manures (Angelidaki et al., 2007).

Introduction

Saffron is a naturally derived plant product from the dried stigma of the Crocus sativus flower  (Gresta et al., 2009).The saffron (Crocus sativus L.) is an Iridaceae family member, herbal perennial, sterile (2n=3x=24) geophyte, traditionally propagated by vegetative corms. Its cultivation dates back to late Bronze Age in the Mediterranean region (Negbi, 1999) in Anatolia since the ancient times (Arslan et al., 2007) and for at least 3500 years in Egypt and Middle East. Saffron contains more than 150 volatile and aroma yielding compounds. The chemical components mainly include carotenoids, crocin, picrocrocin, safranal etc (Abdullaev, 1993, Tarantalis et al., 1995, Karaoglu 
et al., 2007). Saffron is mostly used as spice, food colorant, dye or perfume. The dried stigmas introduce unique color, taste and fragrance and thus making it the world’s most expensive spice (Baghlian et al., 2010). Saffron has been used in folklore herbal medicines for centuries (Basker and Negbi, 1983).

Bioinformatics is a recently emerged frontier discipline, which helps in analyzing biological data with the help of information technology and statistical software packages. In a broader sense, bioinformatics is the application of computer technology in management of biological information. This interdisciplinary scientific tool having no barriers between different branches of science like biology, biotechnology, mathematics, computer science and information technology, has great influence on advanced bio-science research, particularly dealing with genes, proteins and cell biology.

Bioinformatics is the branch of study that deals with the use of information technology (IT) for managing data of biological science. Bioinformatics drew public attention with the announcement of the draft sequence of human genome by the then US President Bill Clinton along with Craig Venter and Francis Collins in 2000 (Uma and Sathyanathan, 2004).

Since the dawn of the Industrial Revolution, mankind has been introducing numerous hazardous compounds into the environment at an exponential rate.  Environmental pollutants have become a major global concern. The modern growth of industrialization, urbanization, modern agricultural development, energy generation, have resulted in indiscriminate exploitation of natural resources for fulfilling the human desires and need, which have contributed in disturbing the ecological balance on which the quality of our environment depends.
 
Technological revolution has brought new changes in products and processes in industry. The waste generated from the development of products and processes are of concern to the environmental list. A variety of pollutants such as heavy metals, xenobiotics, polycyclic aromatic hydrocarbons (PAHs), chlorinated and nitro-aromatic compounds are found to be highly toxic, mutagenic and carcinogenic for living organisms (Zhang and Bennett. 2005; Samanta et al., 2002).

Bioinformatics is a multidisciplinary approach that combines computational and biological expertise to analyze biological data to advance research and development and it drew public attention with the announcement of the draft sequence of human genome by the then US President, Bill Clinton along with Craig Venter and Francis Collins in 2000. Bioinformatics and life sciences research are gaining attention in the government, industries and academic sectors. India was the first country in the world to establish a biotechnology information system (BTIS) network in 1987 through the Dept. of Biotechnology and created an infrastructure that enables it to harness biotechnology through the application of bioinformatics. Through this branch of science, biological data are acquired, stored and then analyzed. Bioinformatics can be used to store huge bioscience data for their future uses. By exploring computational tools, databases and new strategies for pests and diseases, resistant genes can be identified and plants with desired traits can be engineered to enhance agricultural productivity. Bioinformatics can be utilized in two ways for agriculture, viz., (i) at molecular level for application in breeding strategies and (ii) agri-informatics for land use planning, farmers window, early warning system, impact assessment and environmental applications.

ANTIBIOTICS

Antibiotic is a drug used to treat infections caused by bacteria and other microorganisms. Technically an antibiotic is a substance produced by a microorganism that selectively inhibits the growth of another. Penicillin was the first antibiotic discovered by Alexander Fleming in 1928, a significant breakthrough for medical science. Antibiotics had saved thousands of wounded soldiers in the I and II world wars. Also, use of it made surgery safer and increased the average life expectancy of human beings. Antibiotics are among the most frequently prescribed medications in modern medicine. Some antibiotics are ‘bactericidal’, meaning that they work by killing bacteria. Each type of antibiotic affects different bacteria in a different way. Some antibiotics can be used to treat a wide range of infections and are known as ‘broad-spectrum’ antibiotics. Others are only effective against a few types of bacteria and are called ‘narrow-spectrum’ antibiotics.

In recent years, we have witnessed an explosion of biological information and the bioinformatics data arising from the rapid research and unprecedented progress in molecular biology. The novel genomic technologies have revolutionized the drug designing, discovery and development processes in government as well as private pharmaceutical industries through the use of these technologies. Various databases are doubling in size every year and fortunately we have the complete genome sequences of more than 100 organisms. At present, the pharmaceutical industries have clasped genomics as a source of drug targets. They also distinguish that the field of bioinformatics is crucial for validating these potential drug targets and for determining the ones that are most suitable for entering the drug development pipeline. Due to our increased understanding of molecular biology, there has been a change in the way that medicines are being developed. Once upon a time, new synthetic organic molecules were tested in animals or in whole organ preparations. Now this approach has been replaced with a molecular target in which in-vitro screening of compounds against purified, recombinant proteins or genetically modified cell lines is carried out with a high throughput as a consequence of better and ever improving knowledge of the molecular basis of disease.

Bioinformatics can be defined as the application of computer technology for the management and analysis of biological data using computer to gather, store, analyze and merge the biological data. It consists of the organization of many kinds of large-scale biological data, particularly that based on DNA and protein sequence, into web-accessible databases and the methods required analyzing these data. Bioinformatics is a fast growing field within the biological sciences that was developed because of the need to handle large amounts of genetic and biochemical data. The goal of bioinformatics is to uncover the wealth of biological information hidden in the mass of data and to obtain a clearer insight into the fundamental biology of organism. This knowledge can have profound impact on various fields such as human health, agriculture, environment, energy and biotechnology. Bioinformatics involves use of various biological databases, which are archives of consistent data that are stored in a uniform and efficient manner. Biological databases are large collections of data that are relatively different to maintain outside the centers and institutions that produce them. These data are traditionally accessed and analysed using browser-based World Wide Web (www) interfaces. There are two types of databases i.e. Primary database and Secondary database. Primary databases contain information and annotations of DNA and Protein sequences, DNA and Protein structure and DNA and Protein expression profiles catalogued for the end users.

1.    Biological Control of Insect Pests
    
2.    Biocontrol Agents in Vegetables
    
3.    Mass Multiplication of Predators and Parasites
    
4.    Microbial Control
    
5.    Transgenic Plants
    
6.    Biological Control of Diseases
    
7.    Conclusion
        
References

Introduction
 
Natural control strategies that employ biological agents for pest suppression are generally classified as biological control tactics. In conventional usage, this term usually refers to the practice of rearing and releasing natural enemies:   parasites, predators, or pathogens. A slightly broader definition of “bio-control” includes any related management activity that is designed to protect or conserve natural enemies. Biological control is the use of living organisms to maintain pest populations below damaging levels. Natural enemies of arthropods fall into three major categories: predators, parasitoids, and pathogens (Altieri et al., 2005; Mahr et al., 2008).

Abstract

Biological drainage relies on vegetation, rather than engineering mechanisms to remove excess soil water through evapo-transpiration. It is often considered attractive because it requires only an initial investment in site development (planting of a “bio-drainage crop”) and (potentially) returns a benefit when the bio-crop is harvested for fodder, wood or fibre. Presently about one-third of the world’s irrigated area faces the threat of waterlogging. Considering long-term viability of bio-drainage, the systems would be subjected to the same scrutiny as other plant-based biological systems with regards to nutrient and salt-balance and soil considerations. Moreover, large-scale adoption of bio-drainage crops could include considerable tracts of land and socio-economic considerations.

1    Biological Hazards
    1.1    Bacterial hazards
    
2.     Fungal Hazards
    
3.    Viral Hazards
    3.1     Hepatitis A virus
    3.2       Norwalk virus
    
4.    Parasitic Hazards
    4.1     Parasitic worms
    4.2    Protozoan parasites
    
5.    Aquatic Biotoxins
    5.1    Paralytic shellfish poisoning (PSP)
    5.2    Diarrheic shellfish poisoning (DSP)
    5.3    Amnesic shellfish poisoning (ASP)
    5.4    Ciguatera fish poisoning (CFP)
    5.5     Puffer fish (Tetrodotoxic) poisoning (PFP)
    
6.    Pathogen Survival Through Cooking
    
References

ABSTRACT
Rice straw is one of the most abundant lignocellulosic agricultural wastes in India and can be used as a major resource for biogas production. The structure of rice straw is complex and is made up mainly of cellulose, hemicelluloses and lignin. The lignin physically shields the cellulose and hemicelluloses, the main components which can be converted to energy, and hinders the access of microbial enzymes to these carbohydrate moieties. To overcome this making the straw a potential resource of biogas production, effective pretreatment is necessary that can efficiently hydrolyse the straw and make it accessible to cellulolytic enzymes and methanogens. Various pretreatment methods which are employed currently in the market are discussed here. For a process to be economically feasible it is necessary that the pretreatment be efficient as well as cost effective. To gain some insights into this aspect, the techno-commercial feasibility of Biomethanation of rice straw has been discussed.

ABSTRACT
Nisargruna biogas technology has been developed in BARC for processing different types of biodegradable waste. This technology is based on biphasic separation of aerobic and anaerobic stages. Introduction of aerobic predigester has increased the efficiency of the process in terms of enhancing the quality and quantity of methane and also reducing the retention time of the process. Process parameters of the plant were studied and found that the modified design has proved very effective in faster digestion of complex waste. The technology has been commercialised and has spread across different parts of country for processing variety of wastes including kitchen waste, municipal waste, agriculture waste, textile waste and poultry waste. Microbial population from different stages of the plant was also studied and found their ability to convert waste to biogas, containing mainly methane.

Keywords: biogas, biphasic digestion, green energy, methane, microbial fermentation, Nisargruna, waste management

The successful AI program depends on the health of the semen donors followed by hygiene in semen collection, processing, storage and AI methods as well as reproductive health of the females. However, the microbial contamination may not be able to avoid completely but it can be reduced to the minimum threshold level by adopting hygienic procedures.

The hygienic condition of the neat / frozen semen samples can be assessed periodically by enumerating the bacterial load apart from the periodical screening of bucks for the presence of various causative agents in animals and its transfer to the females via semen samples.

Though both viable and total (viable and nonviable) bacterial counts can be done, the viable techniques are more commonly used in diagnostic and food hygiene procedures. The viable bacteria are capable of multiplication with the production of visible colonies on or in agar media. In viable techniques, the assumption is made that one well-spaced bacterial cell gives rise to one colony. The bacterial colonies, rather than bacterial cells, are counted in these techniques. Among the viable counting techniques like Spread plate, Pour plate, Miles, Misra, Filtration are available and each has its own inherent errors, the pour plate counting method is explained as follows:

Introduction

Agriculture is one of the promising sectors which offers marvelous potential for sustainable growth and play a key role in the economy of the country. Many of the foods that are already common in our diet are obtained from plant varieties that were developed using conventional genetic techniques of breeding and selection. Increase in the world population and limited food supply strained researchers to introduce and advance agriculture management and modern technologies along with conventional practices to attain highest possible crop productivity. Agricultural technology is based on the domestication of wild plants to create the crops that we have come to depend upon. Humans invented agriculture approximately 10,000 years ago when they began to harvest and cultivate specific plants to produce food. The improved plant traits selected by early agriculturalists were transmitted genetically to succeeding generations of plants. Both public institutions and private companies are now involved in crop genetic improvement.

Crop yields are affected by a combination of abiotic stresses, biotic stresses, and nutritional factors but abiotic stresses (drought, heat, cold or salinity) are the major factors that prevent crops from realizing their full yield potential (Edmeades, 2009). In traditional approach, breeders grow and cross varieties and then evaluate how the progenies vary in their ability to deal with stresses. The best-adapted plants are then selected for growing in fields exposed to stresses. Biotechnologists have taken advantage of recent advances in biotechnology and functional genomics to genetically engineer crops which can give better yield in adverse conditions than the unmodified ones (Manavalan et al., 2009; Umezawa et al., 2006). Drought, extreme temperatures (high or low), high salinity and cold are the major abiotic stresses that affect plant growth and result in significant yield losses. Although plants have evolved a wide spectrum of programs for adaptation to changing environment, the current understanding of the mechanisms associated with the ability of crops to maintain yield under abiotic stress are poorly understood (Witcombe et al., 2008; Munns et al., 2008; Bartels et al., 2005). New advances in ‘omic’ technologies are providing opportunities for identification of transcriptional, translational and post-translational mechanisms and signaling pathways that regulate the plant response. This chapter describers several examples of how modern crop technologies may be applied to broaden crop tolerance of various abiotic stresses and to increase total biomass.

Environmental constraints that include abiotic stress factors such as salt, drought, cold, extreme temperatures and heavy metalsalready a major limitingfactor for crop productivity and will soon become even more severe due to climate change conditions.Together, these stresses constitute the primary causes of crop losses worldwide, reducing average yields of most major crop plants by more than 50% (Boyer, 1982; Bray et al., 2000; Wang et al., 2003). Current climate change scenarios predict an increase in mean surface temperatures and drought that will drastically affect global agriculture in the near future (Le Treut et al., 2007).
 
Abiotic stresses trigger many biochemical, molecular and physiological changes and responses that influence various cellular and wholeplant processes (Wang et al., 2001, 2003). For example, drought, salinityand low temperature stress lead to reduced availability of water (alsoknown as dehydration/osmotic stress) characterized by a decreasedturgor pressure and water loss (Dhariwal et al., 1998; Boudsocq and Lauriere, 2005). Osmotic stress promotes the synthesis of the phytohormoneabscisic acid (ABA)which then triggers a major change in gene expressionand adaptive physiological responses (Seki et al., 2002; Yamaguchi and Shinozaki, 2006; Shinozaki andYamaguchi-Shinozaki, 2007).

Introduction

Stress in physics is any force applied to an object. Stress in biology is any change in environmental conditions that might reduce or adversely change a plant’s growth or development. ‘Stress’ or ‘pressure’ was introduced into the theory of elasticity as an amount of force for a given unit area. When sufficient force is applied to material, the material bends and the change in length is termed ‘strain’. With increasing stress, the initially linear relationship between stress and strain becomes nonlinear until the proportionality limit, after which the material deforms elastically (it can bend back), then plastically (it cannot bend back) until it ruptures. Since the 1930s, biologists have attempted to apply this terminology to biological systems, albeit the nature of the stresses will vary between nonliving materials and organisms.

Reproductive Biotechnology
    
Artificial Insemination (AI)
    
History and Future Perspectives of Artificial Insemination
    
Semen Cryopreservation
    
Buffalo
    
Goat
    
Cryoprotection and Anti Oxidants
    
Cryopreservation and Genetic Stability of Livestock Spermatozoa
    
Insemination Technique
    
Cattle and Buffalo
    
Sheep and Goat
    
Conception Rate with Artificial Insemination
    
Multiple Ovulation and Embryo Transfer (MOET)
    
Advantages of Embryo Transfer
    
MOET Includes the Following Steps
    
Superovulatory Treatments
    
Insemination
    
Recovery of Embryos
    
Synchronization of Recipients
    
Cattle
    
Buffaloes
    
Goats
    
Horses
    
In vitro Maturation, fertilization and culture of Oocytes
    
Historical Development
    
In Vitro Maturation of Oocytes
    
In vitro Sperm Capacitation
    
Scope
    
Limitations
    
Embryo Cryopreservation
    
Ultrasonography in Reproductive Management
    
Ultrasound-guided Transvaginal Oocyte Pick-up (OPU)
    
Embryo Cloning or Nuclear Transfer
    
Blastomere Separation
    
Embryo Splitting
    
Nuclear Transfer Cloning (NT)
    
Embryonic Stem Cell Complementations
    
Limitations and Future Prospects of Cloning
    
Sexing of Embryos and Separation of X and Y Chromosomes Bearing Sperms
    
PCR  in Sex Determination
    
Transgenic Animal Production
    
Gene Transfer Through Direct DNA Microinjection
    
Gene Transfer with Retroviral-Vector Assistance
    
Gene Transfer through Production of Germ-line Chimeras
    
Parthenogenetic Embryos: An Emerging Tool in Reproductive Biotechnology for Multiplication of Superior Germ Plasm
    
Applications of Parthenogenesis
    
Livestock Genomics and Marker Assisted Selection (Use of DNA Level Markers)
    
Stem Cells
    
Vaccine for Fertility Control and Increase Prolificacy
    
Auto-immunization to Increase Prolificacy
    
Biotechnology of Conservation of Livestock Breed Biodiversity
    
Gene for Prolificity: Booroola Merino
    
Role of Biotechnology in Enhancing Production
    
Milk
    
Biotechnology of Rumen Manipulation
    
Genetically Modified Ruminal Microorganisms

Biotechnology of Animal Feeding
    
Probiotics
    
Biotechnology Crops as Animal Feed
    
Biotechnology of Animal Products
    
Some New Products made Possible by Biotechnology Include

Biotechnology provides significant opportunities and greater effects in horticulture, where even minor changes such as quality, colour, aroma, flavour, structure, disease-pest resistance and postharvest behaviour bring tremendous achievements commercially. Tissue culture, genetic engineering, molecular breeding, biofertilizers, biopesticides and postharvest biotechnology are important aspects of biotechnology in horticulture such as fruit, vegetable, ornamental, spice, medicinal, aromatic and plantation crops.

Tissue Culture

Tissue culture is the true reality of the plant biotechnology. The annual demand of tissue cultured production constitutes nearly 10 per cent of the total world biotech business, amounting to 15 billion US dollars. The global production of tissue culture industry is expanding @ 15% per annum (Chadha, 2000). In India, more than 130 tissue culture units are presently producing > 300 million plantlets per annum (Choudhury and Tejaswini, 2000).

Animal Science Biotechnology Research Centres
    
Current Status and Some of the Achievements in Animal Heatlh
    
National Bioresource Development Board
    
Animal Biotechnology
    
Biotechnology Parks and Incubators
    
National Institute of Immunology, New Delhi
    
Bose Institute, Kolkata
    
Jawahar Lal Nehru University, New Delhi
    
National Centre For Cell Science, Pune
    
Centre for Dna Fingerprinting and Diagnostics (Cdfd) Hyderabad
    
National Institute for Plant Genome Research (Nipgr) New Delhi
    
Institute of Bioresources and Sustainable Development,Imphal
    
Institute of Life Sciences, Bhubaneshwar
    
Bharat Immunologicals and Biologicals Corporation Limited
    
International Centre for Genetic Engineering and Biotechnolgoy (Icgeb), New Delhi
    
Animal Biotechnology Research Abroad

1. Introduction

Stress-Any deviation from this optimal condition of any factor essential for its growth will lead to aberrant change in physiological processes and due to this plant body will experience tension and this state can be referred as plant under stress.

In physics, stress is defined as mechanical force per unit area applied to an object. In response to the applied stress, an object undergoes a change in the dimension, which is also as strain. However, the biological stress is defined as an advance force or a condition, which inhibits the normal functioning and well-being of a biological system such as plants. There are several abiotic and biotic factors, which under a given condition, may be perceived as stress by plants.

4.1. Substrate quality
    Litter quality
    Soil organic matter (SOM) quality

4.2. Decomposer community
    Soil animals
    Soil microorganisms
    Terrestrial fungi
    Actinomycetes    
    Bacteria

India is the largest producer and consumer of pulses in the world, accounting for 33 per cent area and 25 percent production. Pulses are mostly grown under rainfed conditions in marginal to sub-marginal lands. Once a net exporter it is presently one of the largest importer of pulses as our demand outstrips domestic production. While the traditional cropping pattern almost always included a pulse crop either as a mixed crop or in rotation, the commercialization of agriculture has encouraged the practice of sole-cropping. As a result, the annual import has increased from 0.50 million tonnes to 1.80 million tonnes during the last five years and contribution of pulses in the national food basket reduced from 17% to 7%. Pulses are generally grown on marginal and sub-marginal lands under rainfed conditions with low inputs and suffer heavily due to biotic and abiotic stresses, resulting into low productivity. Challenges for pulses in India are decline in area of pulses, low genetic yield potential, low realized yield, instability in production, climate change, biotic and abiotic stresses, poor seed replacement rate, post harvest losses, wide fluctuation in price, no regular MSP/procurement policy, poor availability of critical inputs in productivity zone and poor transfer of technology.

Vegetable is an important constituent of Indian diet. India is the second largest producer of vegetables which contributes 16.7% to global vegetable area with a production of 156.33 million tons (15.4%) (Table 1). The level of productivity of vegetable17.4 tons/ha is quite low (Rai et al., 2014). Majority of Indians are vegetarian, with a per capita consumption 135 g per day as against the recommended 300 g per day. It is still very less than recommended diet level. In near future, there is a need of around 5-6 million tons of food to feed our 1.3 billion Indian population expected by the year 2020 (Paroda 1999). The major biotic stress in vegetable production is due to increased insect pest. In many cases, there is 100 per cent yield loss due to viral diseases vectored by insects. Vegetables are more prone to insect pests and diseases mainly due to their tenderness and softness as compared to other crops and virtual absence of resistance characters because of intensive hybrid cultivation. The insect pests inflict crop losses to the tune of 10-30 per cent in vegetable production (Table 2). To overcome these losses farmers used indiscriminate doses of toxic chemical on vegetable. It accounts for 13-14 per cent of total pesticides consumption, as against 2.6 per cent of cropped area (Sardana, 2005). For the benefit of the farmer and other, application of BIPM is must. Bio intensive pest management involves every part of management that keeps pest below economic injury level so that pest does not cause any economic loss to the farmers or growers.

Abstract

Abiotic stresses have been a serious challenge for sustainable agriculture particularly vegetable cultivation characterized by decreased endurance against stresses like drought, temperature fluctuation and salinity. In India, drought and salinity being major abiotic stresses affect an area of 16% and 4.28%, respectively. Plant Breeders have to face many impediments while developing stress tolerant varieties. Developing tolerant varieties is quite difficult as tolerance varies with the developmental stages and has complex inheritance. This can be understood well by studying ontogeny of plants. Drought varies in time of occurrence, severity and duration. Field screening for tolerance is very difficult because of various interactions like drought × high temperature, high temperature × salinity interactions etc. Artificial screening in different stresses could be an alternative approach. The varieties developed with higher inputs at research stations fail to perform at farmers’ fields due to high genotype × environment interactions. Cultivated species have less genetic variability for abiotic stress tolerance but tolerance can be enhanced by using diverse and heterogeneous population. Though wild species possess genes for tolerance but these genes are closely linked with the undesirable gene(s) e.g. in tomato where S. cheesmanii, S. peruvianum, S. pimpinellifolium and S. sitiens have undesirable traits which affect yield and quality adversely.

Abstract

Modern agriculture has disturbed the age-old balance of host-pathogen system resulting diseases occur at regular interval leading to enormous loss to the agricultural production, which is estimated to be 10-25% or less during normal years but may extend up to 100 % during epidemic. Broadly, there are two breeding approaches adopted for disease resistance which includes conventional and molecular one. Conventional approach includes pureline, pedigree, backcross, recurrent selection and interspecific hybrids through which various resistant varieties has been developed. However, after sometime there is sudden outbreak of diseases due to continuous sowing of these varieties which results in the advancement in molecular biology and with the discovery of trait linked markers it paved a new pathway to look a new corner to develop varieties which are disease resistant. Molecular approach consists of gene pyramiding through which two or more genes providing resistance to different types of diseases is introgressed in a particular variety, gene deployment and multiline varieties which consists of heterogeneous population formed by mixing the cultivars to differ in the ability of disease resistance and reduces the rate of epidemic development. With the help of makers in marker assisted breeding using foreground, recombinant and background selection several linked markers are identified which provides resistance against disease like in wheat stem rust, leaf rust etc.

Ian Donald in the year 1956 used the one-dimensional A-mode (amplitude mode) to measure the parietal diameter of the fetal head and thus introduced the ultrasound in diagnostic and medicine. Ultrasonography does not use radiation or powerful magnetic field as source of energy and the equipment cost is usually affordable. This modality involves the use of sound waves in viewing the longitudinal, sagittal and transverse planes for both testes . Sound is measured in cycles per second or hertz (Hz); the audible range of sound is from 20-20,000 Hz. Diagnostic ultrasound is usually in the range of 1–20 megahertz (MHz).

The speed of sound depends on the tissues through which it propagates because density and compressibility change the speed, the sound wave travels. On an average the assumed speed of sound in soft tissue is 1540 m/s.

India has a mega animal bio-diversity, which is manifested in the form of availability of a large number of species, and a variety of breeds even within species. India is bestowed with rich and varied domestic animal biodiversity having a total of 32 breeds of cattle and 10 buffalo breeds. The diversity of livestock is not only accidental, nor purely natural; it is more the outcome of thousands of years of continuous selection. The trends in livestock population over the last three decades have shown that, there is significant increase in the population of buffaloes i.e. from 60.5 crore in 1973 to 96.9 crore in 2003, while cattle population has increased from 180.1 crore to 226.1 crore in the same period. The share of buffaloes, in the bovine population increased from 22 to 32 per cent while that of cattle has shown declining trend. Within the cattle, there has been marked shift from work animals towards milch animals which has resulted in loss of important draught breeds of cattle. Further indiscriminate crossbreeding has resulted in significant decline in number of important milch breeds like Sahiwal.

Biological and chemical reactions in living cells are dramatically reduced at low temperature, a phenomenon that can lead to the possible long-term preservation of cells and tissues. However, freezing is fatal to most living organisms, since both intra and extracellular ice crystals are formed and results in changes to the chemical setting of cells that lead to cellular mechanical constraints and injury. The major hurdle for cells to overcome at low temperatures is the water-to-ice phase transition. Cell injury at fast cooling rates is attributed to intracellular ice formation, whereas slow cooling causes osmotic changes due to the effects of exposure to highly concentrated intra and extracellular solutions or to mechanical interactions between cells and the extracellular ice. Cryopreservation is a process that maintains biological samples in a state of suspended animation at cryogenic temperature for any considerable period and is used to preserve the fine structure of cells (Jang et al., 2017).

Principal Scientist and I/c Head, Division of Social Sciences and Training, IIHR, Hessaraghatta Lake Post, Bangalore – 560 089, Karnataka

Entrepreneurs have many forms of retail business ownership available to them. Each business model has its own list of pros and cons. Choosing a type of retail business to start will depend on why you want to own a business, as well as your lifestyle, family, personality, basic skills and much more. Here are a few of the main types of retail ownership and the advantages, disadvantages, and support system of each.

Principal Scientist and I/c Head, Division of Social Sciences and Training, IIHR, Hessaraghatta Lake Post, Bangalore – 560 089, Karnataka

The business plan answers the key questions about the business: who, what, where, when, why, and how. For a developing business, it begins the process of making an idea materialize into reality and determining whether or not an idea should become reality. It also establishes goals and benchmarks for the business. A comprehensive business plan makes it easier for a business to receive external support, especially in terms of financing from potential investors, lenders, and grant sources. Finally, and possible most importantly, going through the process of planning is very valuable because it forces the business owners and/or managers to think through every detail of the business.

Introduction

High density planting in fruit trees was first introduced in case of apple in Europe during the nineteen sixties, with the development of Malling and Malling - Merton rootstocks. Subsequently, this was experimented in other temperate fruit crops like peach, plum, pear and sweet cherry. Today, majority of new orchards of apple and to some extent that of other fruit crops in Europe, New Zealand, USA and Australia use this intensive system of fruit production profitably Iyer and Kurian (2006). The successful management of trees in any high-density planting system depends on maintaining a balance between vegetative growth and fruiting.

Canopy management is the manipulation of tree canopies to optimize the production of quality and quantity fruits. The canopy management, particularly its components like tree training and pruning, affects the quantity of sunlight intercepted by trees, as tree shape determines incoming radiation. Light is critical for growth and development of trees and their fruits. The green leaves harvest the sunlight to produce carbohydrates and sugars which are transported to the sites where they are needed – buds, flowers and fruits. Better light penetration into the tree canopy improves tree growth, productivity, yield and fruit quality. The density and orientation of planting also impact light penetration in an orchard (Proietti et al., 2000). Generally, in close planting, quicker shading becomes a problem. An east-west row orientation results in more shading as compared to the western and southern orientation of trees. The problem of a fruit grower is initially to build up a strong and balanced framework of the trees, then equips them with appropriate fruiting.

Introduction

Agroforestry is the science of designing and developing integrated self-sustainable land management system, which involves introduction or retention of woody components such as trees, shrubs along with agricultural crops including pasture/animals simultaneously or sequentially, on the same unit of land and at the same time, to meet the ecological as well as socio-economic needs of local people. A typical agroforestry system will allow economic and ecological interactions between woody and non-woody components and also increase, sustain and diversify the total land output. Agroforestry systems are very important for the area currently under agriculture, the number of people who depend on land for their livelihoods, and the need for integrating food production with environmental services (Garrity, 2004; Makundi and Sathaye 2004). Agroforestry provides a unique opportunity to combine the twin objectives of climate change adaptation and mitigation. Although agroforestry systems are not primarily designed for carbon sequestration, there are many recent studies that substantiate the evidence that agroforestry systems can play a majorrole in storing carbon in above ground biomass (Sathaye et al. 2001;

1    Ruminants
2    Canines

INTRODUCTION

The initial care of young seedlings and propagated plants is very necessary and important. Efforts should be made in such a way that the young plants can be propagated and grown to a saleable size with no insect-pests and diseases attack and nutrient deficiency. The supply of healthy plant material to the growers helps in good reputation of a nursery as well as to make fruit crops more profitable.

Sometimes young nursery plants suffers from various problems like attack of insect-pests and diseases, nutrient deficiency, scanty or excessive moisture and stagnation of excess or rain water in the field. The young seedlings/plants require light and frequent irrigation and need special care to make them grow well and to save them from adverse weather conditions. They must be protected from intense heat and cold. The young nursery plants growing on light or sandy soils can exhibit deficiency symptoms of macro and micro elements. Such nutrient deficiencies can easily be corrected by foliar or soil application of desired nutrient.

1.    Classification of Trees
    
2.    Flowering and Foliage Trees of Indian Gardens
    
3.    Palms and Cycads
    
4.    Coniferous Trees and Deciduous Trees for Temperate Climatic Situations
    
5.    Propagation and Planting of Trees
    
6.    After Care of Trees
        
    Further Reading

1. Introduction

The term nanotechnology was first coined by Norio Taniguchi in 1974 to describe controlled thin film deposition (Taniguchi, 1974). Nanotechnology is now generally recognized to include materials with a size in at least one dimension in the 1-100 nm range, although this terminology is not strictly enforced (Rao, 2008). In food science, there are many types of naturally occurring particles that fall within this size range and the use of a strict definition is problematic due to the large distribution of sizes (Kruif, 1998). Many natural foods contain nanoscale components and their properties are determined by their structure. These have been eaten safely for generations. In fact, some of food’s most important raw materials (proteins, starches, and fats) undergo structural changes at the nanometre and micrometre scales during normal food processing(Morris and Parker 2008). Food proteins (for example, native beta-lactoglobulin, which is about 3. 6 nm in length) can undergo denaturation (via pressure, heat, pH, etc. ) 

INTRODUCTION

Cashew nut (Anacardium occidentale L.), in the current global scenario, has attained the status of economically important crop with considerable export potential. Ancient sea route traders have been responsible for the spread of cashew from Brazil, its native, to other parts of tropical and sub tropical regions. In the rainforest areas with hot and humid regions of North east Brazil that the people from the long past knew to roast and use the cashew kernels. The owning of cashew trees meant the wealth, prosperity and social status of the family even in those days around 1500 AD in Tropical American region. The commercial importance of cashew nuts was not aggressively known at the time of its initial introduction into Asian and African countries.

Plants are often exposed to various abiotic and biotic stresses and have developed specific mechanisms to adapt, survive and reproduce under these stresses (Pieterse et al., 2009). Abiotic stresses include drought, water logging, high temperature, cold(low temperature), salinity, chemical pollution (xenobiotics), uv radiation, heavy metal and oxidative and plants are also challenged by biotic stresses through microbial pathogens such as myco-plasma, nematodes, fungi bacteria (Tippmann et al., 2006). The biology of plant cell is more complicated with any foreign stimulus from the environment; multiple pathways of cellular signaling and their interactions are activated. These interactions mainly evolved as mechanism to enable the plant systems to respond to stress with minimum and appropriate physio- biochemical processes. Abiotic and biotic stress induces signals and theses signals are recognized by receptors, followed by generation of secondary messengers e.g. activation of ion channels, production of reactive oxygen species (ROS) Xiong et al., 2002, accumulation of hormones (Bari and Jones, 2009; Peleg and Blumwald, 2011) such as salicylic acid (SA), ethylene (ET), jasmonic acid (JA) and abscisic acid (ABA). Secondary messengers are responsible for modulating intracellular level of calcium, often initiating protein phosphorylation cascade, which may leads to the activation of various proteins directly involved in cellular protection (Tippmann et al., 2006) Fig.1.