eChapters

Abstract: Trees are known to reduce growth and yield of crops under their canopy in agroforestry systems through competitive interaction. This chapter describes the mechanism of competitive interaction between Linseed (Linum usitatissimum L.) and Leucaena leucocephala (Lam) De Wit in an alley cropping system. To know the mechanism of competitive interaction in agroforestry, three competition situations are created: crop + Leucaena shrub neighbour, crop + L. hedge neighbour and sole crop. In this study competitive interaction is studied in an alley cropping system having two alley width sizes i.e. 4m and 8m. Sunlight at ground, soil organic C and total N are measured in all the competition treatments at different distances from shrub and hedge neighbors. Sunlight is not reduced in crop + hedge treatment like that in sole crop treatment. Sunlight is reduced in crop + shrub treatment (36 to 82%), being the highest in 4m alley and the lowest in 8m alley size. Soil organic C is high but total N is low in crop + hedge and crop + shrub neighbour than in sole crop treatment. Comparing the two competitive treatments (crop + hedge and crop + shrub neighbour) it is observed that growth rate (42 to 55%) and crop yield (71 to 72%) are reduced greater due to aboveground than belowground competition. Belowground competition is 3.6 times greater in 4m than in 8m alley, suggesting that spacing is an important factor, which regulates tree/crop competition so does the crop yield reduction in an agroforestry system.

Introduction

In the present, India is food secure due to our self-sufficiency in foodgrains, but still,we have to achieve the goal of nutritional security. The horticulture sector is the one which alsoescapes the weather shocks and has the potential to help in poverty alleviation, nutritional security. Again, it has ample scope for farmers to increase their income and helpful in sustaining a large number of agro-based industries which generate a large number of employment opportunities besides helping to achieve the national goal of 4.0 per cent agricultural growth. India with more than 28.2 million tonnes of fruits and 66 million tonnes of vegetables, is the second largest producer of fruits and vegetables in the world, next only to Brazil and China.Onshares, the world fruit production is 11% and vegetables 7%.

1.     Preparation of Liquid and Solid Media
    
2.     Isolation of Pure Culture
    
3.     Analysis Bacterial Growth Curve
    
4.     Study of Effect of Carbon Source on Bacterial Growth
    
5.     Gram Staining
    
6.     Aseptic Cell Transfers
    
7.     Prokaryote Cell Number by Dilution Plating
    
8.     Cell Mass by Measurement of Turbidity
    
9.     Isolation of Bactriophage from Sewage Sample
    
10.     Standard Plate Count
    
11.     Imvic Test
    
12.     Antibiotic Sensitivity Test on Microbes
    
13.     Study of Transformation In Bacteria
    
14.     Study of Bacterial Conjugation
    
15.     Effect of pH and Temperature on Bacterial Growth
    
16.     Immobilization of Yeast Cells and Alcohol Production
    
17.     Citric Acid Production using Aspergillus Niger
    
18.     Alcohol Production from Yeast and Estimation of Alcohol

ABSTRACT
Methanogenesis is an important biogeochemical process takes place in anaerobic ecological sites. It is the last step of terminal electron accepting process of anaerobic respiration. Methanogens synthesise CH₄ using acetate and CO₂ as precursor molecules. Former types of methanogens are referred as acetoclastic methanogens and the later ones as hydrogenotrophic methanogens. After biogenesis of CH₄, it is emitted to atmosphere and causes global warming. In aerobic ecosystem CH₄ is oxidised by methanotrophs to CO₂ a less global warming molecule. However, in anoxic environment CH4 acts as an electron donor and interacts with various electron acceptors. Methane modulates denitrification, Fe reduction, SO₄ reduction and organic matter decomposition. Manuscript elucidates methanogenesis and highlights the pathways of interaction between methanogenesis and cycling of electron acceptors in various anaerobic ecosystems in relation to global C and N balance.

ABSTRACT
Methanogenesis is a ubiquitous process primarily encountered in anaerobic environments. Methanogens are a group of diverse obligate anaerobic bacteria that produce methane from complex organic matter. Despite their differences in morphological characters and habitat, they show coherence in terms of methane-producing metabolic activity. Being distinctive from all other classical prokaryotes, these are grouped under another kingdom of Archea. Other distinctions of the members are based on their unique cell wall structures and membrane components, as well as differences in their highly conserved gene sequences. Methanogens likely owe their cosmopolitan status to their unique mode of energy metabolism of methane generation. They are active in various ecological niches, from rumens of animals to submarine volcanic vents, sewage digestors and landfills. Methanogenic bacteria are abundant in habitats where high affinity electron acceptors are negligible. Methane fermentation involves bacteria that obtain energy by catalysing anaerobically degradable organic matter to CH₄ and CO₂ by consuming H₂, CO₂, and low-carbon organic acids as their substrates. The gaseous products of methanogenesis are greenhouse gases, thus are major contributors to global warming. Methanogens could be used to produce renewable carbon-neutral energy substitute, biogas. Methane cycling archaea play a major role in global methane production as energy for the future.

Method of Maximum Likelihood Estimation (M.L.E.)
    
Method of Moments
    
Method of Minimum X²
    
Interval Estimation

An early and accurate diagnosis of pregnancy is a crucial factor for successful management and maintaining cost-effective production in scientific goat production for better economic returns. In addition, accurate information on the stage of gestation would also be useful to dry off lactating females at an adequate period. The techniques for pregnancy diagnosis in goats are mostly depend upon visualization of the conceptus or determination of its secretory products in the maternal blood or the milk are the most defined and specific methods for pregnancy. The choice of pregnancy diagnosis method in goats depends on the stage of gestation. Some methods have a higher degree of accuracy at the early gestation while others do at the late stage of gestation. Assessment of pregnancy in terms of hormonal assay of blood plasma, serum, or milk, and estimation of pregnancy-specific antigens or proteins give a higher level of accuracy within 30 days of pregnancy. There are many methods that are currently being used for pregnancy diagnosis in goats. Among those, the method applied should be safe for both offspring and mother and need to be low-cost and easy to apply. Various techniques have different kinds of limitations to their wide-scale use. These techniques are classified into 3 classes, which are as follows:

Introduction

Hi Tech horticulture is synonymous to cultivation of commercial horticultural crops using advanced scientific technology to make more profit. India is acclaimed globally for production of high value horticultural crops but there productivity is less as compared to many countries. One of the main reasons for low productivity is poor water and nutrient management. The Task Force on Agricultural Development constituted by the National Institution for Transforming India (NITI) Aayog, Government of India in March 2015 has suggested to increase productivity, progress is required along three dimensions: (i) Quality and judicious use of inputs such as water, seeds, fertilizer and pesticides; (ii) judicious and safe exploitation of modern technology; and (iii) shift into high value commodities such as fruits, vegetables, flowers, etc. (Anonymous, 2015).  Hi tech horticulture meeting the entire requirement as suggested by the task force.

A microarray, as the name suggests, is a two dimensional array of microscopic probes which captures certain molecules in an environment by a process known as hybridization, and are used to detect quantities of these molecules in the environment. The probes can vary according to requirement of experiment, and so can the environment, which in technical terms is an extract of tissue under study.

1     Introduction

2     Bacterial Flora on Fish

3     Products of Bacterial Activity

4      Bacterial Flora of Fish Caught from Different Waters

5      Fish Spoilage and Bacterial Flora

6      Bacterial Contaminations
    
7      Bacterial Contamination of Harvested Fish
    
8      Growth of Spoilage Bacteria
    
9      Scombroid Poisoning
    
10      Microorganisms on Iced Fish
    10.1     Crustaceans
    10.2     Molluscs
    
References

Abstract
 

Ensure enough food and water to the growing world population and preserve the biodiversity of our planet are among the major challenges of our immediate future. These challenges are essentially addressed to the agricultural world, which is constantly evolving to satisfy the most basic needs of the humankind. The approaches and the possible answers to these big challenges are many and, even if partial, each sustainable solution or innovation can contribute to improve the quality of our life. To overcome the barriers for food and nutritional security microgreens is one of the possible innovative approach in this 21st century. A microgreen is a young vegetable green that is used both as a visual and flavor component or ingredient primarily in fine dining restaurants. Fine dining chefs use microgreens to enhance the attractiveness and taste of their dishes with their delicate textures and distinctive flavors. Smaller than “baby greens,” and harvested later than sprouts, microgreens can provide a variety of leaf flavors, such as sweet and spicy. They are also known for their various colors and textures. Among upscale markets, they are now considered a specialty genre of greens that are good for garnishing salads, soups, plates, and sandwiches.

Abstract

In the recent past we have seen a great upsurge in the production and productivity of food grains as well as horticultural produce which is sufficient to feed our burgeoning population. This way we are self-sufficient to feed our population, but now the major challenge before us is to ensure the nutritional security of each and every individual of the country. Moreover, meeting nutritional security through conventional or molecular breeding demands long time. However, this could be achieved in much shorter period of time i.e., within 2-3 weeks by growing microgreens.Microgreens can be easily characterised by having a central stem with two fully developed cotyledon leaves and mostly one pair of small true leaves.In comparison to their mature counterparts, microgreens of a variety of vegetables and herbs have been reported to be more nutritious. They are rich in ascorbic acids, carotenoids, phylloquinone and tocopherols, which is responsible for its antioxidant properties and also plays a vital role in weight gain modulation, cholesterol metabolism. It may also protects from cardiovascular diseases by preventing hypercholesterolemia. Main problem associated with microgreens are their high price and high perishability and lack of awareness among people about its cultivation practices and health benefits. It demands great attention from the scientific community, to address these problems and make it available in each and every household to ensure the nutritional security of the nation.

Abstract

Plant research often involves growing new plants in a controlled environment. These may be plants that we have genetically altered in some way or may be plants of which we need many copies all exactly alike. These things can be accomplished through tissue culture of small tissue pieces from the plant of interest. The latest list of ISO recognizes 109 spices, but only a few are important commercially such as black pepper, cardamom, ginger, turmeric, large cardamom, cumin, coriander, fennel, fenugreek, garlic, saffron and celery. They are extensively used in flavouring food and confectionery, perfumery and soaps and also as colouring materials. Many of these groups of crops have medicinal properties and are used in many Ayurvedic and Unani medicines. Inspite of the rich diversity and importance, the productivity of spices is low in India. Spices are an important group of economic plants with large genetic diversity in the Indian subcontinent and need to be conserved through in- vitro methods. Conservation of the germplasm using traditional method of field maintenance is problematic, normally labour intensive and also face risk of germplasm loss due to pest/pathogen attack or natural disasters. To minimize the problems of their conservation and increasing interest has been shown to use plant tissue culture. In vitro conservation of germplasm has been reported in various crop species including the spices black pepper, cardamom, ginger, turmeric, large cardamom.

Abstract

A perennial plant or simply perennial is a plant that lives for more than two years. The term is often used to differentiate a plant from shorter-lived annuals and biennials. The term is also widely used to distinguish plants with little or no woody growth from trees and shrubs, which are also technically perennials. in vitro technologies are continuously expanding in the field of biology. Plant tissue culture has become a general title for a very broad subject. Commercial use of micropropagation is still limited, because of its relatively high production cost resulting mainly from high labor costs, low growth rate in vitro, and poor survival rate of the plantlets during acclimatization. Micropropagation of woody perennials is essential due their importance in production of secondary metabolites, which are playing a vital role now a days. Stages of micropropagation i.e mother plant selection, establishment of the aseptic culture, multiplication, root regeneration and transfer to the natural environment, which are systematically reproduced in many protocols which will aids in micropropagation studies of many woody perennials. Thus the review of present study attempts to provide a comprehensive account on micropropagation of woody perennials complementing conventional programmes for improvement.

Introduction

The world demand for wood is continuously increasing due to increase in population. The ever increasing population has led to depletion of existing timber reserves and the forestland base has been reduced by increased allocation of forest areas to urban, agricultural, recreational and other uses. The growing awareness towards development of plantations has generated a voluminous increase in the demand for quality planting material. Conventionally, propagation is achieved through sexual (seeds) or vegetative methods (rooting of cutting, grafting, layering, etc). Propagation through seeds is routinely used for production of planting material but it generates immense genetic variability which is not desirable while propagating perennial tree species. In order to propagate selected trees with desirable features vegetative propagation methods like rooting of cuttings, grafting, layering etc have been developed. These methods can produce genetically identical off springs thereby preserving the advantageous traits;

By definition, minimal processing would encompass any procedure, short of traditional complete preservation procedures (heat sterilization etc.) that adds value to the fruit or vegetables (Floros, 1993). Other terms used to refer minimally processed products are lightly processed, partially processed, fresh processed, fresh cut and prepared. Minimally processed fruits and vegetables are products those have the attributes of ‘fresh-like’ quality. A number of minimally processed products of fruits and vegetables are now being produced like peeled and sliced potatoes, shredded lettuce and cabbage, washed and trimmed spinach, chilled peach, mango, banana, melon and other fruit slices, vegetable snacks such as carrot and celery stick  and cauliflower and broccoli florets, packaged mixed salads, cleaned and diced onion, peeled and cored pineapple, fresh sauces, peeled citrus fruits and microwaveable fresh vegetable trays. Minimal processing of fruits and vegetables has two purposes. First, it is important to keep the product fresh but convenient without losing its nutritional quality. Second, the product should have a shelf life sufficient enough to facilitate distribution within the region of consumption. The microbiological, sensory and nutritional shelf life of minimally processed fruits and vegetables should be at least 4-7 days but preferably even longer (Ahvenainen, 2000).

Introduction

Agroforestry, the word coined in the early seventies, has made its place in all the developed and the developing countries of the world and is now recognized as an important approach to ensuring food security and rebuilding resilient rural environments (Luedeling et al. 2014).  Agroforestry applies to private agricultural and forest lands and communities that also include highly erodible, flood-prone, economically marginal and environmentally sensitive lands (Continents 1986). The typical situation is agricultural, where trees are added to create desired benefits. Agroforestry allows for the diversification of farm activities and makes better use of environmental resources (Taylor et al. 2007). Agroforestry is defined as a land use system which integrates trees and shrubs on farmlands and rural landscapes to enhance productivity, profitability, diversity and ecosystem sustainability. It is a dynamic, ecologically based, natural resource management system that, through the integration of woody perennials on farms and in the agricultural landscape, diversifies and sustains production and builds social institutions. India has been an all-time leader in agroforestry (Chavan et al. 2015).

Stress, is usually defined as an external factor that exerts disadvantageous influence on the plant. In most cases, stress is measured in relation to plant survival, crop yield, growth (biomass accumulation), or the primary assimilation processes (CO2 and mineral uptake), which are related to overall growth. Some environmental factors (such as air temperature) can become stressful in just a few minutes; other may take days to become stressful or even months (some mineral nutrients) to become stressful. Different types of abiotic stresses that affect fruit production are; water stress, heat stress, chilling and freezing stress, salinity stress, flooding stress. Although, it is convenient to examine each of these factors separately, many are interrelated. For example, water deficit is often associated with salinity in the root zone/ or with heat stress in the leaves. Plants often display cross resistance, or resistance to one stress induced by acclimatization to another. This behaviour implies that mechanism of resistance to various stresses share many common features.

Plants encounter adverse environmental stresses during their life cycle, which have negative impacts on growth and greatly affect crop productivity. As perennial crops, fruit trees are exposed to an array of stresses for a long time once they are planted. If the trees are severely injured by environmental stresses, it would be hard for them to recuperate, leading to retarded growth and reduced fruit production. Furthermore, the negative effects of the stresses are not limited to the fruit production in the current year but can also extend to the next year(s). Therefore, it is of critical importance to develop techniques for reducing stress injury and/or improving stress tolerance in fruit trees for sustainable cropping, which can be fulfilled by the cultivation method or genetic engineering (Holmberg and Bulow, 1998). 

1. Introduction

Cherry peppers (Capsicum frutensis var. cerasiforme) which is locally known as dallae khorsani, is one of the important cash crops of Sikkim and Darjeeling hills. They are small, round shaped, fleshy and heavily seeded. They are green in color in early stage of fruiting and turn red when fully matured. It is an excellent source of vitamin A and C, which are important antioxidants (Howard et al., 1994). Cherry peppers are an ideal size for pickling or brining. Its hotness ranges from moderately mild to very high.

Due to its high moisture content (about 85% w. b. ), shelf life of cherry pepper is limited to 7 - 10 days. This makes it rare and costly during the off season. Hence, preservation of cherry peppers is of considerable importance both to the farmers as well as to the consumers. Drying is one of the oldest methods for preservation of cherry peppers to make it available throughout the year. To increase shelf life of chilies, drying up to 10 % moisture content (dry basis) is required which is accepted in export market.

For drying chillies, sun and hot air drying are the most common drying method used. However, due to the prolonged drying time of 16-48 h, the quality of the dried chillies is reduced (Kim et al., 2002). Many researchers paid more attention to innovative techniques such as short and medium-wave infrared radiation drying (SMIR) which not only maintain product quality, but also increase the drying rate. Moreover, infrared drying could save up 50% energy compared to convective drying (Nowak & Lewicki, 2004). The ability of infrared radiation to penetrate and heat the inner layers results in higher rate of heat and mass transfer (Ginzburg, 1969).