eBook Chapters

Definition

Garret (1970) defined biological control as any condition under which or practice whereby survival or activity of a pathogen is reduced through the agency of any other living organism (except man) with the result that there is a reduction in the incidence of the disease caused by that pathogen.

Baker and Cook (1974) stated that the biological control is the reduction of inoculum density or disease producing activities of a pathogen or parasite in its active or dormant stage, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonists, or by mass introduction of one or more antagonists.

Biocontrol can be simply defined as the application of living organism to control diseases.

Managing plant diseases is necessary to preserve the quantity and quality of food, feed, and fiber that producers worldwide produce. To avoid, lessen, or manage plant diseases, several strategies may be employed. Aside from using effective horticultural and agronomic techniques, growers frequently largely depend on chemical pesticides and fertilizers. The remarkable increases in crop productivity and quality over the past 100 years have been largely attributed to such agricultural inputs. However, fear-mongering by certain opponents of pesticides and environmental degradation from excessive and improper use of agrochemicals have prompted significant shifts in public perceptions toward pesticide usage in agriculture. Political pressure is present to remove the most dangerous chemicals from the market, and there are stringent limits on the use of chemical pesticides today. Furthermore, because of the potential scale at which such applications could need to be made, the proliferation of plant diseases in natural ecosystems may make the successful application of pesticides impossible. As a result, several researchers studying pest management have concentrated on creating substitutes for artificial chemicals in the management of diseases and pests. Biological controls are one type of alternative among them.

COMMON FEATURES OF BIOLOGICAL MEMBRANES

Membranes are sheetlike, just a few molecules thick and form closed boundaries between cell compartments.

Membranes contain lipids and proteins, with small amounts of carbohydrayes linked to the lipids and proteins.

Lipids in membranes are small with hydrophobic and hydrophilic portions. Lipid bilayers provide a barrier to the diffusion of polar molecules.

Characteristic functions of membranes are mediated by specific proteins, serving as pumps, channels, receptors, energy transducers and enzymes.

Membrane components associate through noncovalent interactions.

Membranes are asymmetrical, with two sides of the membrane differing from each other.

Lipid and protein molecules often diffuse rapidly in the plane of the membrane.

Central transport of ions and molecules into and out of the cell.

Generate proton gradients for ATP production by oxidative phosphorylation.

Receptors bind extracellular signals and transduce the signal to cell interior.

Biological control in plant pathology refers to the use of living organisms to suppress or control plant pathogens, pests, and diseases, thereby reducing the need for chemical pesticides. These living organisms, known as biological control agents (BCAs), can be naturally occurring or introduced, and they act by various mechanisms to inhibit or eliminate the pathogen. These methods harness natural enemies of pathogens such as antagonistic fungi, bacteria, or other beneficial organisms to reduce the population of harmful pathogens in the environment. Biological control is an important component of sustainable agriculture, offering an eco-friendly alternative to chemical pesticides and contributing to integrated disease management (IDM).
Key Mechanisms of Biological Control
1. Predation: Certain organisms, like Trichoderma species or predatory mites, directly attack and consume plant pathogens, reducing their population.
2. Parasitism: Some fungi, like Ampelomyces quisqualis, parasitize plant pathogens, such as powdery mildew, thereby suppressing their growth and reproduction.
3. Antibiosis: Microorganisms like Bacillus subtilis and Pseudomonas fluorescens produce antibiotics that inhibit the growth or kill plant pathogens. For example, Bacillus subtilis produces iturin, which suppresses fungal growth.

Nanoparticles synthesis is done by two major routes, which include classical synthesis and green synthesis. The green synthesis techniques generally utilize relatively non-toxic chemicals non-toxic solvents, biological extracts and systems. Biological methods are considered safe and ecologically sound forthe nanomaterial fabrication asan alternative to conventional physical and chemical methods

Nanoparticles

Gold, silver, and copper have been used mostly for the synthesis of stable dispersions of nanoparticles, which are useful in areas of photography, catalysis, biological labeling, photonics, optoelectronics and surface-enhanced Raman scattering (SERS) detection

The need for biosynthesis of nanoparticles rose as the physical and chemical processes were costly. So in the search of cheaper pathways for nanoparticle synthesis, scientists used microorganisms and then plant extracts for synthesis. Nature has devised various processes for the synthesis of nano- and micro- length scaled inorganic materials which have contributed tothedevelopment of relatively newandlargely unexplored area of research based on the biosynthesis of nanomaterials.

17.1 Introduction

Bioenergy is an array of energy produced by biomass (organic matter) including liquid, solid, and gaseous fuels, electricity, heat, steam, biochemicals and materials. Biofuel is any plant derived organic matter available on a renewable basis, including dedicated energy crops and trees, agricultural food and feed crops, agricultural crop wastes and residues, wood wastes and residues, aquatic plants, animal wastes, municipal wastes, and other waste materials. An organic matter such as wood, plants, residues from agriculture or forestry, and the organic component of municipal and industrial wastes that can now be used as an energy source. Today, many bioenergy resources are replenished through the cultivation of energy crops, such as fast-growing trees and grasses, called bioenergy feedstocks.

6.1 Introduction

Nanotechnology is the study of manipulating matter on an atomic or a molecular scale that deals with particle sizes between 1 and 100 nano meters at least in one dimension. Particles reduced to the nanoscale show some unusual properties which are different from what they exhibit on a macro scale, enabling unique systematic applications Tarafdar et al. (2012). The unexpected properties of nanoparticles are broadly due to the large surface area of the material, which dominates the contributions made by the very small quantities of the material. Nanoparticles, thus, take advantage of their dramatically increased surface area to volume ratio.

Nanotechnology has the potential to revolutionize the agricultural and food industry with new tools for the molecular treatment of diseases, rapid disease detection, nanofertilizer, enhancing the ability of plants to absorb nutrients etc. Nanotechnology opened doors to new ways of identifying and quantifying biomolecules through use of nanosensors and nanoprobes.

Introduction

After water, nitrogen is the most critical nutritive element for crop productivity and human efforts to produce food and energy have greatly changed the nitrogen cycle of the earth. Sustainable cropping systems throughout history and across the world have relied on the combination of a cereal with an N-fixing legume. In recent history, cereals have dominated global agriculture, while legume areas and productivity have stagnated or even declined. An atmosphere around us contains nearly 78% nitrogen, which is in free form. Hence it is not available to plants. At present, India produces around 206 million metric tones of foodgrains for its growing population, increased foodgrain cannot be produced unless we carefully make use of biological nitrogen. Biological nitrogen fixation is the key to sustain agricultural productivity through the application of biofertilizers in the field. However, there is an urgent need to transfer this technology on the field to farmers and industry by producing these fertilizers on large scale.

Abstract

Nitrogen (N₂) is an essential nutrient for every organisms. It is the building block of many biological molecules. Atmosphere contains 79% of N₂ gas which cannot be used by plants directly as it is chemically inert. Many diazotrophs like bacteria and cyanobacteria have the capability to convert atmospheric dinitrogen into a usable form by means of Biological nitrogen fixation (BNF). N₂ fixation is generally three types i.e. Asymbiotic, Associative and Symbiotic. Asymbiotic N₂-fixation also known as free living N₂-fixation is under taken by some bacteria and cyanobacteria. In associative N₂-fixation, some bacteria fix nitrogen in association with root of some grasses and cereal plants. In symbiotic nitrogen fixation, Rhizobium form root nodule in legumes and fix atmospheric N₂ and convert it into the ammonia (NH₃) by the help of Nitrogenase enzyme. This oxygen sensitive enzyme consist of two components i.e. di-nitrogenase (MoFe protein) and di-nitrogenase reductase (Fe protein) which are highly conserve in sequence and structure. The N₂ fixing genes e.g. nod gene (nodulation gene) and the nif gene (nitrogen-fixing gene) are located in the plasmid which is also called mega plasmid. Symbiotic association between legume and rhizobium have agronomic importantance and reduce the use of N-fertilizers or even avoided. The long term use of the nitrogenous biofertilizer in the agricultural field is a promising approach to develop and fulfil N₂ demand of the growing crop without causing any environmental hazard.

Abstract
Biological nitrogen fixation (BNF) is a critical ecological process carried out by grain legumes, which form symbiotic associations with nitrogen-fixing bacteria, primarily Rhizobium species. This natural mechanism converts atmospheric nitrogen (N2) into ammonia, a form that plants can readily utilize, significantly enhancing soil fertility and reducing the need for synthetic nitrogen fertilizers. The process begins with the recognition and infection of the legume roots by rhizobia, leading to the formation of specialized structures called nodules, where the bacteria convert atmospheric nitrogen into ammonia through the enzymatic action of nitrogenase. The symbiosis between legumes and rhizobia is tightly regulated by various genetic and environmental factors, including plant signalling molecules, soil conditions, and nutrient availability. Grain legumes, such as soybeans, chickpeas, and lentils, play a vital role in sustainable agriculture by improving soil health, promoting biodiversity, and providing a protein-rich food source. This overview explores the molecular mechanisms underlying BNF, the factors influencing the efficiency of this process, and its potential to enhance sustainable agricultural practices. Understanding the intricacies of BNF in grain legumes can inform breeding programs and management strategies aimed at maximizing the benefits of this natural process for agricultural productivity and environmental sustainability.
Keywords: Nitrogenase, Biological Nitrogen Fixation, Environmental sustainability, Soil fertility

Fixation of elemental nitrogen in the atmosphere by the microorganisms through a reductive process into ammonia is called biological nitrogen fixation (BNF). Molecular nitrogen (N₂) is the major component (approximately 80%) of the earth’s atmosphere. The element nitrogen is an essential part of many of the chemical compounds, such as proteins and nucleic acids, which are the basis of all life forms. However, N₂ cannot be used directly by biological systems to build the chemicals required for growth and reproduction. Before its incorporation into a living system, N₂ must first be combined with the element hydrogen. This process of reduction of N₂ commonly referred to as nitrogen fixation (N-fixation).

N₂is transformed into reactive N via lightning discharge (5 teragrams (Tg) N / year; 1 Tg equals 1012 g) and by biological N fixation (100 -140 Tg N /year ). Conversion of dinitrogen to ammonia in the industrial process requires temperatures of 400 - 500^o C and high pressures (Haber-Bosch process). Biological nitrogen fixation is much more important as it contributes to more than 70 % of the input in to the world’s soil and water nitrogen. It has been estimated that 100 to 175 million tones of nitrogen are fixed annually by the process of biological nitrogen fixation.

The process of N₂ fixation is mediated by the enzyme, called nitrogenase (which reduces N₂ to ammonia). The overall reaction is as follows.

The biochemical oxygen demand is a chemical procedure for determining the amount of dissolved oxygen needed by aerobic organisms in a water body to break the organic materials present in the given water sample at certain temperature over a specific period of time. BOD of water or polluted water is the amount of oxygen required for the biological decomposition of dissolved organic matter to occur under standard condition at a standardized time and temperature. Usually, the time is taken as 5 days and the temperature is 20°C. microbes
C6H12O6 + 6O2 ¾¾¾¾®CO2 + H2O
Principle
The test measures the molecular oxygen utilized during a specified incubation period for the biochemical degradation of organic material (carbonaceous demand) and the oxygen used to oxidize inorganic material such as sulfides and ferrous ion. It also may measure the amount of oxygen used to oxidize reduced forms of nitrogen (nitrogenous demand).
Procedure
Fill two BOD bottles with sample (or diluted sample); the bottles should be completely filled.

Phytoplankton populations are important in shrimp ponds because they aid in shrimp nutrition and the removal of excess nutrients from the pond environment. They can be beneficial or have potentially negative consequences, depending on how they are managed.

    Introduction
Over 60% of the population in India is occupied in agriculture and associated fields, making it one of the world’s most agriculturally dense nations. In India, the agricultural sector accounts for 18.6% of the country’s GDP. Almost all crops, including cereals, vegetables, and cash crops, are grown in India (Vanitha et al., 2013; APEDA, 2020). From the beginning of time, several pests, including weeds, insects, plant diseases, and nematodes, have had a detrimental effect on agriculture. As a result, crop losses are estimated to be 45%, or over 290 billion, annually (Aneja et al., 2016). Regardless of tactics, illnesses and pests significantly contribute to agricultural losses. Diseases, weeds, and insects hinder plant growth and lower plant density, eventually lowering the food supply. The Entomological Society of America (ESA) has released a policy statement stating that invasive insects cost more than $2.5 billion to control and cost $18 billion per year in economic losses to pastures, crops, lawns, and forests (The Not-So-Hidden Dangers of Invasive Species, 2018). Invasive crop pests continue to threaten food security because of the intricate relationships between human migration, international trade, climate change, and evolving agricultural techniques. Several of these variables also contribute to the sharp rise of urban pests and disease-carrying insects. The primary method of insect pest management has been chemical pesticides. Nonetheless, an increasing call for less agricultural chemical use due to worries about the environment, human health, and pesticide resistance ignites interest in novel ways.
Conventional chemical pesticides have raised food production and harmed non-target animals and the ecosystem. To prevent and mitigate pest-induced crop losses in storage (post-harvest losses) and in the field (pre-harvest losses). This has been accomplished through the development of numerous crop protection techniques. In the past few decades, chemical pesticides have been utilized to reduce food losses induced by insect pests; however, this has decreased agricultural production. Moreover, volatile pesticide residues have periodically raised questions about food safety among domestic customers and presented challenges to export crops’ ability to trade. Insecticides can cause physical or biological harm to pests. Certain insecticides are applied topically or indirectly to plants those insects may consume. Pesticides harm nature, soil, and human well-being, so regulations and rules governing their use are becoming more stringent. New technology and products to control and prevent pests are desperately needed. In modern agriculture, various methods are now available, including biological control, insect behavior, genetic modification, microbial pesticides and plant immunization of pest populations.

1. Introduction

Mounting pressure of teeming population and shrinking land resources have necessitated the production of increasingly more food per unit area. Insect pests, diseases and weeds are important biotic constraints inflicting 20-25% loss in agriculture production. Synthetic pesticides have of course, played a very significant role in restricting many pest problems. However, their indiscriminate has been its toll by creating several problems like pesticide resistance in insects, contamination of food with toxic residues, resurgence of pests and deleterious effects on non target organisms. The number of effective pesticides available is dwindling day by day further having less choice among which to choose. These ill effects have arised interest in alternate methods of plant protection.

The extent to which various plant species are utilized for pest control depends, not only on their effectiveness against target pests, but also on socio-economic, environmental and policy considerations. Keep these in mind the present study on the efficacy of botanicals against Spodoptera litura was carried out.

Abstract

Electronic waste or e-waste is one of the emerging problems in developed and developing countries worldwide. The constantly changing today’s world of technology has led to the serious problem of e-waste. The previous studies show that India has generated 0.6 million tons of e-waste in 2014 which may increase to 0.7 to 0.9 million tons by 2015–2016. E-waste constitutes multiple components some of which are toxic that can cause serious health and environmental issues if not handled properly. The challenge is to develop innovative and cost-effective solutions to decontaminate polluted environments, to make them safe for human habitation and consumption, and to protect the functioning of the ecosystems which support life. Biological approach is currently applied to recovery of metals from contaminated soil, groundwater, surface water, and sediments including air.

Tissue or cell samples from a living organism, different cell cultures grown in a laboratory incubator under controlled conditions, homogenates or extracts of cells and tissues, solutions of isolated and purified components (e.g. proteins, nucleic acids) can all be referred to as biological samples. As the medium of life is water, the majority of biological samples can be defined as aqueous solutions with one or more components, colloidal systems, or water-based suspensions (e.g. bacterial cells dispersed in a liquid medium). Consequently, most biochemical experiments also take place in aqueous environments. Different solids (e.g. chemical substances obtained from different companies, synthetic oligonucleotides or peptides) are also often necessary for biochemical research. In most cases, solids are dissolved in water (or sometimes in other solvents) prior to the experiments. Therefore, the methods of preparing solutions and measuring accurately the weight of the required solids will also be discussed below. Sometimes we use gases in the laboratory. These can be stored in gas cylinders (e.g. O₂), in Dewar flasks in liquid state (e.g. liquid nitrogen), or dissolved in water (e.g. HCl or NH₃). By working with gases it is very important to follow all safety instructions to avoid fire, explosions, frostbite or (in case of inhalation) asphyxia or poisoning.

Abstract

Mine spoils pose adverse conditions for soil microbes and plant growth, due to its low organic content and other essential nutrients, unfavourable soil chemistry and poor soil texture. Poor microbial population inhibits nutrient transformation, consequently the establishment of the plants and ultimately affects the process of ecological succession. A synergy among different groups of beneficial microbes accompanied by plantation of suitable tree species on mined over area has proved to be effective for speedy and efficient revitalization of the mined affected lands. Inoculation of plantation with efficient strains of native microbes accelerates the soil redevelopment and helps to establish nutrients cycle readily which attracts native flora thereby the pace of natural regeneration is increased. It is therefore imperative to consider the application of effective microflora in restoration of mined lands along with the plantation.

Aim

To synthesize gold (Au) nanoparticle using fungal mycelium.

Introduction

Biosynthesis of nanoparticles is a kind of bottom up approach where the main reaction occurring is reduction/oxidation. The fungal enzymes are usually responsible for reduction of metal compounds into their respective nanoparticles.

The history of nanotechnology in the modern world dates back to 1959 when American physicist Richard Feynman inspired the scientific community in his lecture “There is a plenty of rooms at the bottom”. Research on nanosized particles dramatically developed into an important field of modern research with potential effects in electronics to medicine and recently in agriculture and food industry.

Nanoparticles exhibit unique properties in terms of chemical, physical, photoelectrochemical and electronic properties when compared to their respective bulk materials. The applications of these nanosized particles are defined in a bigger ways in the field of Biotechnology. All these applications require custom based synthesis methods for specific applications.

Aim

To synthesize phosphorus (P) nanoparticles from tricalcium phosphate using fungal mycelium.

Introduction

Green nanotechnology includes a range of processes that reduce or eliminate toxic substances to restore environment. Microorganisms like fungi and bacteria having naturally bestowed property of reducing and oxidizing nanoparticles, are used for synthesis.

Introduction to the Salmonid Family (Trout’s and Salmon)
The Salmonidae constitute the only extant family of ray-finned fish in the order Salmoniformes, with 11 genera and more than 200 species collectively referred to as "salmonids" or "salmonioids". This family includes all coldwater mid-level predatory fish found in subarctic and cool temperate waters in the Northern Hemisphere, such as char, graylings, freshwater whitefish, taimens, lenoks, and salmon (both Atlantic and Pacific species). In addition to being the Exclusively Distributed by NIPA GENX Electronic Resources and Solution Pvt. Ltd. New Delhi eponym of its genus, the Atlantic salmon (Salmo salar), has also been given the names of its family and order as well. Among teleost fish, salmonids have a relatively primitive appearance, with pelvic fins in the rear and an adipose fin at the rear. There is a single row of sharp teeth in their mouth, along with rounded scales and forked tail fins (Mc Dowell, 1998). Salmonids vary greatly in size, and the smallest reach only 13 cm (5.1 in) when adults, while the largest reaches 2 m (6 ft 7 in) (Pauly et al., 2008). Salmonids are migratory fish that spawn in the shallow gravel beds of freshwater headstreams, develop into juveniles in rivers, creeks, small lakes, and wetlands, and then migrate downstream to spend the majority of their adult lives in larger bodies of water upon reaching maturity. Often euryhaline, salmonid species migrate to the sea or brackish estuaries as soon as they reach adulthood. Just for breeding purposes, they migrate back to the upper streams. Some freshwater salmonids that migrate only between lakes and rivers are referred to as potamodromous, whereas the life cycle of these sea-run salmonids is known as anadromous. In the middle food chain, salmonids are carnivorous predators that eat smaller fish, crustaceans, aquatic insects and larvae, tadpoles, and occasionally fish eggs (even those belonging to their own species), which are then eaten by larger predators. Salmonids are regarded as keystone organisms that contribute biomass to freshwater and terrestrial ecosystems as a result of their mass migration from oceanic to inland waterbodies. Three lineages of salmonids are currently recognized, each classified as a subfamily: Coregoninae (freshwater whitefish), Thymallinae (graylings), and Salmoninae (trout, salmon, char, taimens, and lenoks). Each of the three lineages is generally accepted to belong to a monophyletic group based on derived traits (McPhail and Strouder, 1997).
Salmons and trouts are well-known for their economic importance as both sport fish and commercial products. Trout and salmon stocks are stocked worldwide each year for sport and commerce. Millions of salmon and trout are hatched, raised, and stocked every year as part of government investment programs. To meet market and restaurant demands for trout, a large private industry has developed—especially in Denmark, Japan, and the U.S. Scientists are confronted with the challenge of perpetuating and increasing the abundance of salmon and trout because of the increasing human population and industrial and agricultural demands on rivers. Among heavily populated regions, the demand for trout outstrips the supply. As a result, state and federal agencies in the United States have initiated massive programs to raise and stock trout in heavily fished waters. The artificial abundance of trout, however, is not a good substitute for the natural abundance of the fish.

Introduction
Schizothoracinae, commonly known as snow trouts, are a specialized group of fish thriving in the fast-flowing, snow-fed streams and lakes of regions such as Afghanistan, Pakistan, the Himalayas in India and Nepal, China, and parts of the former USSR. Adapted to these challenging environments, Schizothoracinae have not been extensively studied due to the difficult terrain they inhabit. In India, they are primarily found along the Himalayan range, with an exception in the Periyar, Kerela (river and lake). The Indian Schizothoracinae are classified into seven genera, forming a significant part of Exclusively Distributed by NIPA GENX Electronic Resources and Solution Pvt. Ltd. New Delhi the Himalayan fishery. Resembling Salmonidae, they are commonly referred to as "Indian Trout." Early mentions of Schizothorax species by Day (1877, 1889) were brief. Subsequent taxonomic studies by researchers such as Hora (1921, 1934, 1937, 1953), Misra (1949), and Mukerji (1936) have provided a more comprehensive understanding of their taxonomy.
The Schizothoracinae, part of the Cyprinidae family, represent a diverse group uniquely adapted to high-altitude environments and have been categorized into three groups based on their morphological characteristics: "primitive group," "specialized group," and "highly specialized group" (Cao et al., 1981). The primitive group includes Schizothorax and Aspiorhynchus, which share more traits with the outgroup Barbodes hexagonolepis. They are distinguished by the presence of uroneuralia, three or four rows of pharyngeal teeth, minimal scale degeneration, and a lack of pronounced sexual dimorphism. The specialized group includes Ptychobarbus, Gymnodiptychus, and Diptychus, and is marked by the absence of uroneuralia, one or two rows of pharyngeal teeth, and moderate scale degeneration. The highly specialized group, which comprises Gymnocypris, Oxygymnocypris, Schizophygopsis, Chuanchia, and Platypharodon, is characterized by the complete absence of barbels and scales and a well-developed canalis preoperculomandibularis (Qi et al., 2012, Yonezawa et al., 2014). The genus Schizothorax comprises 63 species in total. Among these, 34 species are mainly found in Central Asia, while 28 species inhabit the Indo-Himalayan region. The species of Schizothorax in the Himalayan region include S. curvifrons, S. nasus, S. richardsonii, O. sinuatus, S. planifrons, S. esocinus, Schizothoraichthys progastus, S. longipinnis, S. kumanonensis, O. molesworthii, S. hugelli, S. labiatus, and S. micropogon (Menon, 1999; Mishra, 2003; Zhang et al., 2018; Ma et al., 2020).

The larval phase is among the most delicate and significant periods in the development of fish and shellfish. It represents the shift from the embryonic stage to early juvenile life and greatly influences survival, growth, and the overall productivity of aquaculture ventures. During this stage, the larvae exhibit distinct morphological, physiological, and behavioral traits, which differ considerably from those of adult organisms.
Newly hatched larvae are usually microscopic and underdeveloped, depending initially on internal energy reserves like the yolk sac for nourishment. After this endogenous feeding phase, they transition to feeding on external sources such as plankton, rotifers, and Artemia. Timely access to suitable food is crucial, as any delay can result in starvation and large-scale mortality.
Fish larvae undergo rapid structural changes as they develop, including the formation of fins, a functional mouth, and a digestive system. Likewise, shrimp and crab larvae progress through multiple stages—nauplius, zoea, mysis, and post-larva—each with specific needs for food and habitat.
Physiologically, larvae have high energy demands and poorly developed respiratory systems, making them extremely sensitive to environmental factors such as oxygen, ammonia, and temperature. Their weak immune systems and fragile exteriors make them prone to disease and stress from handling.
Larval feeding is influenced by visual and environmental cues, and due to their small mouth size, they require micro-sized live feeds early on. Hatcheries must, therefore, maintain live feed cultures and gradually transition larvae to formulated diets as they grow.
Effective hatchery practices depend heavily on an understanding of larval biology. Parameters like light exposure, stocking density, water conditions, and sanitation directly impact larval development. Even minor changes in temperature

1. Giant tube worms - Riftia sp.

2. Novel mussels Bathymodiolus sp.

3. The stock inside the marine protected areas is called as Bank account.

4. Cartilaginous fishes don't have swim bladder.

5. Non- endemic species - Grass carp.

6. Banned species - Tilapia.

7. Non-parasite fish - Echinoderms.

8. Non- Penacid fishery - Maharashtra.

9. Maximum shellfish and finfish exploiting state in India is Kerala.

10. Small reservoir more in Tamil Nadu.

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

I. INTRODUCTION
The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:
1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.
The job of postharvest technologist is to understand life in fruits, vegetables and flowers and prolong their life as much as possible. The post harvest life of produce depends on the rate at which they use up their stored food reserves and their rate of water loss. The sugar and starches (carbohydrate derivatives) are stored in various parts of plants through a process known as photosynthesis occurring in growing plants and are used later to provide energy for further growth, development and to prolong post-harvest life. The process of photosynthesis in growing plants and respiration in harvested produce can be seen in Fig. 2.1. The released energy through process known as respiration occurs in plants before and after harvest. Thus, principal normal physiological processes leading to aging are respiration and transpiration.

Except lions, the tigers like other felids are highly solitary and however, during the breeding period, the males will approach the female for mating activity. Tigers are highly territorial in nature. Like other felids, retractile claws are present in felid which is an anatomically peculiar feature. They are crepuscular in nature and however, they are quite active in the morning hours.

Life cycle of nematode has six stages. The egg stage, first stage juvenile (J₁), second stage juvenile (J₂), third stage juvenile (J₃), fourth stage juvenile (J₄) and the adult stage.

The first four stages are the immature stages and are known as juvenile stages. The female lays eggs in soil or in plant tissues, singly or in groups as egg mass that hatch out into juveniles which are almost similar to adults appearance. The first moult occurs within the egg shell and the second stage juvenile comes out by rupturing the egg shells as J₂. In case of Xiphinema index, the juveniles are reported to emerge from the egg before the first moult. The juvenile cuticle is shed after each moult.

The egg

The nematode eggs are oval in shape. The eggs are covered by three membranes, the external protein layer which is the secretion of uterus wall, the middle chitinous layer or the true shell secreted by egg itself and the inner lipid layer (vitelline layer). The chitin content in the egg shell vary in different species of nematodes.

Life cycle of nematode has six stages. The egg stage, first stage juvenile (J1), second stage juvenile (J2), third stage juvenile (J3), fourth stage juvenile (J4) and the adult stage.

Introduction

The biology is the study of life forms, subdivided into a variety of specialist categories that include their morphology, physiology, anatomy, behavior, origin, and distribution.The conservation of endangered species is often equated with the preservation of biodiversity by people all over the world. Although endangered species are among the most obvious and understandable markers of the continual loss of biodiversity, ecologists are aware that biodiversity involves much more than just endangered species. An increasingly popular and crucial component of initiatives to preserve the ecological diversity of the planet is the protection of such species.

Endangered species

Endangered (EN): A taxon that is not critically endangered but facing a very high risk of extinction in the natural environment in the near future, it is considered to be endangered.

The important criteria are

1. A decline of at least 50% over at least 10 years or three generations that has been observed, estimated, inferred, or suspected. 2. Population thought to consist of no more than 2500 mature individuals.

28.1 Introduction

The use of biological light in active transport, nerve transmission, and muscle contraction is a feature shared by all living organisms. Surprisingly, a large number of living organisms have evolved the ability to convert chemical energy into light, which is manifested as bioluminescence.

Bioluminescence is a fascinating biological phenomenon found in both terrestrial and aquatic living organisms. The light emitted by living organisms as a result of chemical reactions in their bodies is known as bioluminescence. Cold light is produced by this type of chemiluminescence.

Biomass briquetting is the process of compressing loose and low-density biomass materials such as sawdust, agricultural residues, and forest waste into high-density, solid fuel blocks called briquettes. The process involves mechanical compression under high pressure using machines like piston presses, screw extruders, or hydraulic presses, depending on the technology used.
The main purpose of briquetting is to increase the density of biomass to improve its combustion properties, handling, transportation, and storage efficiency. In most modern systems, briquetting is done without the use of binders, as natural lignin present in the biomass acts as a binding agent under pressure and heat.
Importance of Biomass Briquetting
Biomass briquetting holds immense environmental, economic, and social importance.
1. Environmental Protection
• Briquetting reduces deforestation by providing an alternative to firewood and charcoal.
• It minimizes air pollution by producing cleaner-burning fuel with lower particulate emissions and sulfur content.
• The process helps in carbon sequestration by ensuring the
sustainable use of biomass.
2. Waste Management
• Agricultural residues like rice husk, sugarcane bagasse, cotton stalks, and sawdust, which otherwise cause disposal problems, are effectively utilized.

Biomass is a renewable and valuable resource that has many uses, including energy production, biofuels, and bioproducts. It’s made up of a complex mixture of organic compounds like cellulose, hemicellulose, lignin, proteins, fats, and minerals, which can vary depending on the source and type of biomass. Understanding the composition and properties of biomass is essential for efficient use. In this chapter, we’ll provide an overview of how to analyze and evaluate biomass as a renewable energy source. We’ll cover various techniques and methodologies for analyzing biomass, including physical, chemical, and thermal properties. We’ll discuss the importance of biomass composition, moisture content, ash content, elemental analysis, calorific value, and other relevant parameters when assessing its suitability for energy conversion. Additionally, we’ll highlight the significance of biomass characterization in understanding biomass feedstock variability and its impact on energy production efficiency, environmental performance, and sustainability.

1.1 General

Biomass is a renewable and plentiful energy source with enormous potential to meet the rising demand for clean and sustainable energy solutions. This chapter offers an overview of biomass and its conversion technology, which includes various techniques to transform biomass into useful forms of energy like heat, electricity, and biofuels.

Biomass is an organic substance obtained from plants, animals, and microorganisms. It can be utilized as a source of energy or as a basic material in various applications. The energy in biomass is derived from photosynthesis, during which plants convert sunlight, carbon dioxide, and water into organic matter. Since it comes from living or recently deceased organisms, it is a sustainable and renewable resource. In terms of carbon dioxide emissions, biomass is considered a carbon-neutral energy source because the carbon dioxide produced during combustion is offset by the carbon dioxide absorbed by plants during their growth.

5.1 Introduction

The densification of biomass involves transforming loose and low-density organic materials, such as wood residues, agricultural waste, and plant-based materials, into compact and energy-dense forms. This process is necessary because natural biomass can be challenging to handle, transport, and store. Densification uses mechanical, thermal, or chemical processes to increase the material’s density and energy characteristics. The resulting products, such as pellets, briquettes, or cubes, offer several advantages, including higher energy content per unit volume, efficient storage and transportation, reduced moisture content, and improved combustion efficiency. These products are utilized in various industries, including energy production, residential heating, waste management, and industrial applications. Biomass densification promotes sustainable practices by effectively utilizing biomass resources, reducing waste, and promoting the use of renewable energy sources while mitigating environmental impacts.

2.1 Introduction

Biomass store solar energy in the chemical form and it is most precious and versatile resources on earth. Biomass, unlike fossil fuels, is a renewable energy resource that is available where the climatic conditions are favorable for plant growth and production¹. The term biomass is used for all organic materials which are combustible in nature, mainly plant and animal origin present in land and aquatic environments. Biomass is considered carbon neutral, because the amount of carbon it can release is equivalent to the amount it absorbed during its life time. There is no net increase of carbon to the environment in the long term when combusting the lignocellulosic materials. Therefore, we can say that biomass is a renewable source of energy and can play vital role in responding to concerns over the protection of the environment and the security of energy supply^2,3.

Biomass energy accounts for about 15% of the world’s primary energy consumption and about 38% of the primary energy consumption in developing countries⁴. Furthermore, biomass often accounts for more than 90% of the total rural energy supplies in developing countries⁵. It is the main source to meet out domestic energy in developing countries⁶. The efficient use of fuel wood is much more eco-friendly than the use of efficient and conventional fuels like kerosene and liquefy petroleum gas (LPG). LPG emits 15 times more CO₂ per kg than wood, and kerosene emits nearly 10 times as much. CO₂ is the prominent candidate for global. It is commonly assumed that biomass fuel cycles based on renewable in nature and greenhouse-gas (GHG) neutral because the combusted carbon in the form of CO₂ is soon taken up by re-growing vegetation⁷.

12.1 General

The term “biomass” generally refers to renewable organic matter generated by plants through photosynthesis. Materials having organic combustible matter is also referred under biomass. Biomass is an important fuel source in our overall energy scenario. Biomass is produced through chemical storage of solar energy in plants and other organic matter as a result of photosynthesis. During this process conversion of solar energy in sugar and starch, which are energy rich compounds takes place. The chemical reaction of photosynthesis can be written as:

6CO₂ + 6H₂ O + sun light → C₆ H₁₂ O₆ + 6O₂ + 636_kcal

It indicates that the storage of 636 kcal is associated with the transfer of 72 gm carbon into organic matter. Biomass can be directly utilized as fuel or can be converted through different routes into useful forms of fuel. In fact, biomass is a source of five useful agents, which start with `F’ like Food, Fodder, Fuel, Fiber and Fertilizer. Further, biomass has many advantages like.

5.1 Introduction

Biomass energy is the energy obtained from biomass or living things. Biomass is renewable organic material that comes from plants and animals. Plants generally produced through the process of photosynthesis as shown in Figure 5.1. Biomass is organic, meaning it is made of material that comes from living things, such as plants and animals. Wood, plants, and waste are the most common biomass energy sources. These are called biomass feedstocks. A plant absorbs the sun’s energy through photosynthesis and converts carbon dioxide and water into nutrients (carbohydrates). The energy from these materials can be transformed into usable energy through direct and indirect means. Biomass can be burned to create heat (direct), converted into electricity (direct), or processed into biofuel (indirect).

7.1 Introduction

Biomass gasification is age old technology originated in 1800 century for lighting and cooking purposes (Sansaniwal et al., 2017). Biomass typically contents biopolymers such as cellulose, hemicellulose and lignin in addition to the presence of average composition of C₆H₁₀O₅ which depends upon physical characteristics of biomass¹. Oxygen is required for combustion of every fuel. For complete combustion of biomass stoichiometric air to fuel ratio required varies from 6:1 to 6.5:1 with CO₂ and H₂O as end product.

Biomass gasification is an efficient and environmentally friendly way to produce energy². Gasification process is nothing but it is a conversion of solid fuel into gaseous fuel for wide applications. This whole process completed at elevated temperature range of 800-1300 ^oC with series of chemical reaction that is why it come under thermo chemical conversion³. Thermo chemical processes are most commonly employed for converting biomass into higher heating value fuels⁴. Major thermal conversion route is include direct combustion to provide heat, liquid fuel and other elements to generate process heat for thermal and electricity generation is summaries in Fig. 7.1.

A biomass gasifier is an advanced device designed to convert solid biomass materials-such as wood chips, crop residues, saw-dust, coconut shells, and other organic matter-into a combustible gas mixture known as producer gas. The conversion process, known as gasification, is a thermo-chemical process that takes place in a controlled environment with limited oxygen. Unlike complete combustion, gasification allows only partial oxidation of biomass, producing a gas rich in carbon monoxide (CO), hydrogen (H2), methane (CH4), and nitrogen (N2). This gas mixture can be used as a fuel for internal combustion engines, power generation, cooking, or industrial heating applications.
Principle of Gasification
Gasification involves a sequence of four key thermo-chemical reactions within the reactor, occurring at temperatures ranging from 800–1000°C:
1. Drying Zone: Moisture present in the biomass is evaporated due to the rising heat.
H2O (liquid) ? H2O (vapor)
2. Pyrolysis Zone: Biomass decomposes in the absence of oxygen to form volatile gases, tar, and solid char.
Biomass ? Char + Volatile gases + Tar
3. Oxidation Zone: A small amount of air or oxygen is introduced to partially combust the biomass, generating heat to sustain the process.

Biomass, a diverse and renewable resource, holds immense potential as a source of energy, feedstock, and environmental solutions. However, effectively harnessing its benefits necessitates a systematic approach. This chapter introduces the concept of Biomass Management Techniques as a pivotal framework for optimizing the utilization of biomass resources. With a focus on sustainable practices, this chapter delves into various techniques to handle, process, and utilize biomass efficiently. From collection and transportation to storage, conversion, and beyond, Biomass Management Techniques encompass a wide array of methods that ensure the seamless integration of biomass into energy generation, industrial processes, and environmental conservation.

6.1 Introduction

Biomass pellets are one of the most common types of biomass fuel. It is generally composed of wood waste, agricultural biomass, grasses, and forest residues, and is one of the most popular types of biomass fuel available today. Pelletization of biomass results in significant savings in terms of transportation, and storage, as well as the ease of handling biomass as a whole. In terms of flowability, dense cube pellets are similar to cereal grains in terms of their characteristics of being dense and cube-shaped. Due to their regular geometry and small size, biomass pellets are easily fed through an automatic feed system with fine calibration thanks to their regular shape. Because pellets are so dense, they can be stored in a compact way and transported over a long distance in a rational and efficient manner, because of their density. Furthermore, pellets can also be produced with a low moisture content, even though they are extremely dense. This allows them to burn with a high degree of combustion efficiency due to their low moisture content.

1.1 General

The term “biomass” generally refers to renewable organic matter generated by plants through photosynthesis. Materials having organic combustible matter is also referred under biomass.Biomass is an important fuel source in our overall energy scenario. Biomass is produced through chemical storage of solar energy in plants and other organic matter as a result of photosynthesis. During this process conversion of solar energy in sugar and starch, which are energy rich compounds takes place. The chemical reaction of photosynthesis can be written as:

6CO₂ + 6H₂O + sunlight → C₆H₁₂O₆ + 6O₂ + 636kcal

It indicates that the storage of 636 kcal is associated with the transfer of 72 gm carbon into organic matter. Biomass can be directly utilized as fuel or can be converted through different routes into useful forms of fuel. In fact, biomass is a source of five useful agents, which start with ‘F’ like food, fodder, fuel, fiber and fertilizer. Further, biomass has many advantages like.

(1) It is widely available

(2) Its technology for production and conversion is well understood.

(3) It is suitable for small or large applications

(4) Its production and utilisation requires only low light intensity and low temperature (5 to 35°C)

(5) It incorporates advantage of storage and transportation

(6) Comparatively, it is associated with low or negligible pollution.

1.    Introduction    232
2.    Photosynthesis and Biomass Yields    232
3.    Biomass Properties    233
    3.1.     Polymeric and chemical composition, 233
    3.2.     Fuels analysis, 235
    3.3.     Physical properties, 236

The weight of living plant material contained above and below a unit of ground surface area at a given point of time, is known as plant “Biomass”. Biomass is of utter importance because it provides energy source (Roberts et al. 1987).

Biomass, which has dimensions of mass per unit, is expressed as g m^-2, kg ha^-1 or t ha^-1.

Measurement of Biomass

The area (in the crop field) under study is divided into a number of equal squares or rectangular plots measuring 1.0 m² or 0.5 m². Each square is designated with a particular number. Sample can be harvested from a small area or quadrate at the centre of each square; the remaining peripheral area serving as buffer zone (Wiegert, 1962).

Depending upon the growth pattern of plant species two different designs have been adopted for the sampling:

8.1 Introduction

Nanotechnology is the manoeuvring or self-assembly of individual atoms, molecules, or molecular clusters into structures to craft materials with new properties. The definition of nanotechnology is based on the prefix “nano” which is from the Greek word meaning “dwarf”. In more technical terms, the word “nano” means 10^-9, or one billionth of something. The word nanotechnology is generally used when referring to materials with the size of 1 to 100 nanometres, which is true for metallic nano-material. In case of polymeric nano-material, size may reach 600 nm, as polymeric materials are larger in size compression to metallic material. Nano-materials have different properties from bulk materials as a result of their size. These differences include physical strength, chemical reactivity, electrical conductance, magnetism, and optical effects (Schmid, 2006).

Animals experience the effects of force in everyday life. For example, the animal body exerts a force on the body and to the objects during various agricultural operations. Furthermore, the gravitational force exerts continuously, for instance while sitting, walking, etc. All parts of the body in one way or the other are loaded by forces. The bones provide rigidity to the body and can sustain high loads. The skin is resistant to force. The cardiovascular system is continuously loaded dynamically owing to the pulsating blood pressure. The bladder is loaded and stretched when it fills up. The intervertebral discs serve as flexible force- transmitting media that give the spine its flexibility. Beside force, animals are using levers all the time in their daily life to increase the ‘force’ that is applied to some object.
Animal soft tissues are complex materials that can exhibit nonlinear, time dependent, inhomogeneous, and anisotropic behaviors. The mechanical responses are reflected in all levels of organism (molecules, organelle and body levels). Several biological activities are under the action of forces, which even govern the gene expression. The study dealing with the forces and their effects on living systems is the Biomechanics.
Biomechanics applies the laws and the techniques of mechanics in the study of biological systems and phenomena. Indeed, mechanics provides the tools for:

Introduction

The term "nano" is used to define one that has a size in the nanometer range. Nanotechnology is an interdisciplinary field of medicine, chemistry, physics, and biology that has emerged during the past few decades as a potential area of study. The goal of this interdisciplinary technology is to transform molecules into entities, then do the opposite. Nanobiotechnology is the term for the fusion of nanotechnology with biotechnology. In nanobiotechnology nanoscale principles and techniques are used to explore biosystems and generate novel biomaterials. Biosensing, cell labelling, and molecular imaging can all gain benefit from nanostructure materials in life science, health and biomedicine.